Bacteriological quality of drinking water at point of use and hand hygiene of primary food preparers: implications for household food safety

2016 ◽  
Vol 6 (2) ◽  
pp. 224-230
Author(s):  
S. G. D. N. Lakshmi Reddi ◽  
R. Naveen Kumar ◽  
G. M. SubbaRao ◽  
M. Vishnu Vardhana Rao ◽  
R. V. Sudershan
Keyword(s):  
Drinking Water ◽  
Food Safety ◽  
Hand Hygiene ◽  
Low Income ◽  
Water Samples ◽  
Faecal Coliforms ◽  
E Coli ◽  
Point Of Use ◽  
Drinking Water Samples

Quality of water for consumption and food processing activities is universally accepted as an essential component to ensure food safety at household (HH) level. Along with safe water, hand hygiene is also an important factor for reducing diarrheal illnesses. This was a cross-sectional study conducted in rural and urban HHs to detect hygiene indicators in drinking water samples at point of use (PoU) (n = 150) and their association with the hand hygiene of primary food preparers (n = 150). Overall, 24.7% and 9.3% of drinking water samples (PoU), 48% and 20% of hand rinse samples were contaminated by faecal coliforms and E. coli, respectively. Both drinking water (PoU) and hand rinse samples collected from rural HHs showed higher contamination, followed by those from urban slums and low income HHs. Significant association (p < 0.05) and probable risk with faecal coliforms (OR. 2.5; 95% CI: 1.1–5.4) and E. coli (OR. 14.5; 95% CI: 4.1–50.7) was found between hand rinses and drinking water samples that had bacteriological contamination. These results suggest that there was an extensive cross contamination at HH level. So, targeted education is essential on safe food/water handling practices in HHs to prevent food safety risks.

scholarly journals Occurrence of Escherichia coli and faecal coliforms in drinking water at source and household point-of-use in Rohingya camps, Bangladesh

Gut Pathogens ◽  
2019 ◽  
Vol 11 (1) ◽  
Author(s):  
Zahid Hayat Mahmud ◽  
Md Shafiqul Islam ◽  
Khan Mohammad Imran ◽  
Syed Adnan Ibna Hakim ◽  
Martin Worth ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Human Consumption ◽  
Risk Category ◽  
Human Beings ◽  
Faecal Coliforms ◽  
E Coli ◽  
Point Of Use ◽  
Quality Of Water

Abstract Background Safe water is essential for life but unsafe for human consumption if it is contaminated with pathogenic microorganisms. An acceptable quality of water supply (adequate, safe and accessible) must be ensured to all human beings for a healthy life. Methods We collected and analyzed a total of 12,650 drinking water samples, for the presence of Escherichia coli and faecal coliforms, from a large habitation of the displaced Rohingya population comprising of about 1.16 million people living within 4 km2. Results We found that 28% (n = 893) water samples derived from tubewells were contaminated with faecal coliforms and 10.5% (n = 333) were contaminated with E. coli; also, 73.96% (n = 4644) samples from stored household sources (at point of use—POU) were found contaminated with faecal coliforms while 34.7% (n = 2179) were contaminated with E. coli. It was observed that a higher percentage of POU samples fall in the highest risk category than that of their corresponding sources. Conclusions From our findings, it appears that secondary contamination could be a function of very high population density and could possibly occur during collection, transportation, and storage of water due to lack of knowledge of personal and domestic hygiene. Hence, awareness campaign is necessary, and the contaminated sources should be replaced. Further, the POU water should be treated by a suitable method.

Contamination of drinking water between source and point-of-use in rural households of South Africa and Zimbabwe: implications for monitoring the Millennium Development Goal for water

2006 ◽  
Vol 1 (2) ◽  
Author(s):  
Stephen W. Gundry ◽  
James A. Wright ◽  
Ronan Conroy ◽  
Martella Du Preez ◽  
Bettina Genthe ◽  
...  
Keyword(s):  
South Africa ◽  
Drinking Water ◽  
Millennium Development Goal ◽  
Significant Deterioration ◽  
Development Goal ◽  
E Coli ◽  
Safe Water ◽  
Point Of Use ◽  
Quality Of Water

Aims: To assess contamination of drinking water in rural Zimbabwe and South Africa Methods: We conducted a cohort study of 254 children aged 12-24 months in rural South Africa and Zimbabwe. In dry and wet seasons, we measured water quality, using the indicator organism E. coli, at improved and unimproved sources, in household storage and drinking cups. We also recorded hygiene and socio-economic factors for each household. Results: For improved sources, samples with E. coli counts less than 10 cfu/100ml were as follows: at source: 165 (88%); in household storage 137 (59%); in drinking cups 91 (49%). The corresponding values for unimproved sources were: source 47 (29%); household storage 32 (19%); drinking cups 21 (18%). This significant deterioration in microbial quality of water from improved sources was seen in both countries and both survey rounds. Conclusion: Although improved sources generally delivered ‘safe’ water at the point-of-supply, 12% of source samples were contaminated and as such were ‘unsafe’. Furthermore, in household storage, more than 40% of samples were ‘unsafe’. For monitoring the Millennium Development Goal for water, UNICEF-WHO are assuming an equivalence between ‘improved’ sources and ‘safe’ water. Our findings suggest that this equivalence may be unsound.

scholarly journals Point-of-Use Water Treatment Methods and the Microbiological Quality of Drinking Water in a Peri-Urban Community of Bonendale, Douala IV, Cameroon

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Ngouakam H ◽  
◽  
Fru Cho J ◽  
Tientche B ◽  
◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Microbiological Quality ◽  
Coliform Bacteria ◽  
Cross Sectional ◽  
Potential Health ◽  
Coliform Count ◽  
Point Of Use ◽  
Dug Wells

Background: The study aimed at assessing the knowledge of Bonendale populations related to safe drinking water; determine the microbiological quality and the household treatment practices of drinking water at the point of use (PoU). Methods: The study was descriptive and cross-sectional, conducted between November 2017 and November 2018 in Bonendale, Douala IV. A systematic random sampling method was used to select 237 households. Results: The main source of drinking water in the study population was dug wells (64.9%). The major activity around the boreholes was agriculture 50% (6/12) while agriculture and livestock rearing were practiced around 34.3% (12/35) and 42.8% (15/35) of dug wells respectively. Nearly half 110 (46.4%) of respondents had a good knowledge of physical properties of drinking water. Fifty-nine water samples out of a total of 60 tested (98.3%) were contaminated by coliform bacteria. Analysis of water samples indicated that 47.6% (10/21) of dug wells, 37.0% (10/27) of Camwater, and 41.2% (5/12) of boreholes had a total coliform count varying between 101-1000 CFU/100mL. And only a single Camwater water sample 1 (137%) had a coliform count of 0 CFU/100mL. Conclusion: These findings indicated the poor microbiological quality of point water used by Bonendale populations representing an important potential health risk of water-borne diseases. The results also reveal that a very high proportion of households treat their PoU before drinking.

scholarly journals Analysis of heavy metals and coliform in samples of drinking water collected from municipal ward offices of western suburbs of Mumbai, India

2014 ◽  
Vol 15 (1&2) ◽  
pp. 99-107
Author(s):  
G.V Zodape
Keyword(s):  
Heavy Metals ◽  
Drinking Water ◽  
Water Samples ◽  
Fecal Contamination ◽  
E Coli ◽  
Metallic Sheen ◽  
Differential Medium ◽  
Drinking Water Samples

14 drinking water samples were collected from Vile Parle to Dadar of Suburban’s of (P-South ward offices of B.M.C (Bombay Municipal Corporation) Mumbai in the month from June to December 2012. The samples of drinking-water were analyzed for Cu, Zn, Mn, Fe, As, Cr, Ni, Pb, Cd and Hg. From the results so obtained, the contamination due to heavy metals – Zn (3.115 ppm and 7.816 ppm), Mn (3.115 ppm and 7.426 ppm), Fe (1.124 ppm and 2.872 ppm), As (0.011 ppm to 0.091 ppm), Cr (0.188 ppm and 0.998 ppm ), Pb (1.587 ppm and 4.56 ppm) and Cd (0.011 ppm and 0.051 ppm ) was found to be high whereas the contamination due to Cu (0.012 ppm and 0.313 ppm), Ni (0.126 ppm and 0.774 ppm), were found below the acceptable limits and no Hg was detected in the samples of drinking-water. In the present work, MacConkey Broth was used as a differential medium for detection and enumeration of coliforms from a wide variety water samples. The presence of positive doubtful presumptive test immediately suggests that the water is non potable (i.e., both acid and gas develops in a tube after 48 hours incubation). Confirmation suggests that there is fecal contamination in the water under investigation and hence it is non potable. All the fourteen samples (streaked from positive Ma cConkey broth tubes) were found to be contaminated with E. coli, which was further confirmed by the presence of colonies with green metallic sheen observed under a microscope confirmed that the said samples were contaminated with E. coli - the major indicator of fecal contamination.

scholarly journals Bacteriological Examination of Water supply and Drinking Water at Household level in Makalbari Area by the Most Probable Number Method

2021 ◽  
Vol 23 (2) ◽  
pp. 146-152
Author(s):  
Bharati Shakya ◽  
Supri Raj Shrestha ◽  
Vinutha Silvanus
Keyword(s):  
Drinking Water ◽  
Water Supply ◽  
Water Samples ◽  
Most Probable Number ◽  
Drinking Water Source ◽  
Probable Number ◽  
Household Level ◽  
Two Samples ◽  
Drinking Water Samples

Water safety and quality are fundamental to human development and well-being. Yet, at least 2 billion people are using a drinking water source contaminated with feces worldwide. This study was conducted to assess the types of water supply, storage and methods of purification of drinking water and to examine the bacteriological quality of supplied and drinking water at household level by most probable number (MPN) test in Makalbari area in Gokarneshwor Municipality, Kathmandu. A total of 80 households were selected using simple random sampling technique and two samples (one from main water supply and another from drinking water, n=160 water samples) were collected from each household. Ethical clearance was taken from the Nepal Medical College Institutional Review Committee. SPSS version 16.0 was used for statistical analysis. Majority of water supply samples 65 (81.2%) were from tap, 11 (13.8%) were from well and 4 (5.0%) samples were from bore well. Bacteriologically, of the total water supply samples, 34 (42.5%) samples were excellent, 16 (20.0%) samples were suspicious and 30 (37.5%) samples were unsatisfactory by MPN index quality. Most of the households stored drinking water in the tanks 34 (42.5%). Most of the water samples 29 (36.3%) were directly consumed from the water supply without purification. Among the directly consumed drinking water samples, 13 (44.8%) samples showed unsatisfactory MPN index. Most commonly used methods of water purification was filtration 21 (26.2%). Of the total drinking water samples, 33 (41.25%) samples were positive for total coliform. Thirty-two (40.0%) of the households reported diarrhea in past one year. A highly significant association was found between MPN index of drinking water and occurrence of diarrhea in the households. The quality of the most of the water samples from water supply and drinking water were unsatisfactory. Inspite of this, drinking water was not purified in some of the households. Moreover, few of the drinking water samples were not potable even after purification. Therefore, community should be made aware to improve quality of drinking water in households.

Situation of microbiological contamination in bottled drinking water products and some influencing factors in Hau Giang province in 2020

2021 ◽  
Vol 05 (04) ◽  
pp. 47-60
Author(s):  
Thi Thuy Loan Vo ◽  
Thi Tuyet Hanh Tran
Keyword(s):  
Drinking Water ◽  
Food Safety ◽  
Influencing Factors ◽  
Water Production ◽  
Microbiological Contamination ◽  
Factors Affecting ◽  
E Coli ◽  
Bottled Drinking Water ◽  
Drinking Water Production

Background: Bottled drinking water has become popular for consumption by customers. Bottled drinking water with pathogenic microbiological contamination is a public health concern. This study aimed to describe the current situation of microbiological contamination in bottled drinking water products, food safety conditions and some influencing factors in production facilities in Hau Giang province in 2020. Methods: A cross-sectional study has been conducted in 2020 using quantitative and qualitative methods. The evaluation was carried out at 54 bottled drinking water production facilities, 108 workers/owners and 54 samples collected from facilities for microbiological analysis in the province. In-depth interviews were conducted with local government officials on food safety, two owners of facilities and two workers. Results: It showed that the proportion of microbiological contamination in bottled drinking water accounted for 18.5%, of which P. aeruginosa contamination was 18.5%; and Coliform contamination was 3.7%; No drinking water samples were contaminated with E. coli. 79.6% of facilities met general food safety conditions. There were several factors affecting the proportion of microbiological contamination of bottled drinking water as follows: Bad compliance with food safety in production for the sake of profit by workers, affecting the quality of products such as trash cans without lids; No sterilization treatment before filling; Non-sterilization in filling room. Non-monitoring of UV lights for replacement; No regular cleaning of water pipes, tanks, and discharge valves; Difficulty in state management of food safety for facilities of bottled drinking water production, such as insufficient manpower, lack of equipment and funding for activities. Conclusion: The quality of bottled drinking water in Hau Giang province was not good. The important factors affecting the contaminated bottled drinking water products were identified. We recommend that: management agencies should frequently conduct sudden inspections and supervisions of facilities with no ensured food safety and strictly handle according to regulations. Owners of bottled water facilities should voluntarily comply with food safety. This study is particularly concerned with ensuring food safety conditions in the production of bottled drinking water to prevent products from microbiological contamination. Keywords: Microbiological contamination; E. coli, Coliform, P. aeruginosa; bottled drinking water; food safety conditions; Hau Giang province.

Assessment of microbiological quality of drinking water from household tanks in Bermuda

2008 ◽  
Vol 54 (6) ◽  
pp. 495-500 ◽  
Author(s):  
B. Lévesque ◽  
D. Pereg ◽  
E. Watkinson ◽  
J.S. Maguire ◽  
L. Bissonnette ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Membrane Filtration ◽  
Microbiological Quality ◽  
General Information ◽  
Total Coliforms ◽  
E Coli ◽  
Electoral Register ◽  
Highly Correlated

Bermuda residents collect rainwater from rooftops to fulfil their freshwater needs. The objective of this study was to assess the microbiological quality of drinking water in household tanks throughout Bermuda. The tanks surveyed were selected randomly from the electoral register. Governmental officers visited the selected household (n = 102) to collect water samples and administer a short questionnaire about the tank characteristics, the residents’ habits in terms of water use, and general information on the water collecting system and its maintenance. At the same time, water samples were collected for analysis and total coliforms and Escherichia coli were determined by 2 methods (membrane filtration and culture on chromogenic media, Colilert kit). Results from the 2 methods were highly correlated and showed that approximately 90% of the samples analysed were contaminated with total coliforms in concentrations exceeding 10 CFU/100 mL, and approximately 66% of samples showed contamination with E. coli. Tank cleaning in the year prior to sampling seems to protect against water contamination. If rainwater collection from roofs is the most efficient mean for providing freshwater to Bermudians, it must not be considered a source of high quality drinking water because of the high levels of microbial contamination.

scholarly journals Ascertaining the Bacteriological Quality of Water Drawn from Cast and Non-cast Wells in Zaria, Nigeria

2020 ◽  
pp. 34-43
Author(s):  
Danjuma, Florence Yachim ◽  
Onaji, Ajegba Innocent ◽  
Ocheme Julius Okojokwu ◽  
Danladi Yakubu Papi
Keyword(s):  
Public Health ◽  
Drinking Water ◽  
Water Samples ◽  
Dry Season ◽  
Safe Drinking Water ◽  
Internal Wall ◽  
Bacterial Counts ◽  
E Coli ◽  
Bacteriological Quality

Lack of access to safe drinking water is a threat to public health and wellbeing of the people as it exposes them to risk of waterborne diseases. This study assessed the bacteriological quality of well water samples obtained from Zaria communities and compared the level of contamination between wells with internal wall casting (cast wells) and wells without internal wall casting (non-cast wells). Water samples from 142 wells were collected for a period of six months encompassing both rainy and dry seasons (July to September for rainy season and November to January for dry season). The water samples were collected and analysed for faecal contamination using membrane filtration technique. The water samples contained high density of bacterial counts ranging from 1.73x103±2.73x102 – 2.02x102±1.85x102cfu/100ml during the raining season and 4.17x102±3.07x101 – 1.39x103±3.27x102cfu/100ml during the dry season. Coliforms and E. coli were present in all the samples with  total coliform counts ranging from 1.06 x 103 – 2.12 x 104 cfu/100 ml and E. coli counts ranging from 5.10 X 102 – 5.0 X 104 cfu/100 ml Counts were higher during the raining season in all the locations compared to the dry season. Faecal coliform counts were higher in wells without casting (91.9%) compare to wells with casting (91.2%) while E. coli counts were higher in wells with casting (77.9%) compared to wells without casting (66.2%). There was no significant difference in the bacterial counts between cast and non-cast wells (p-values were all greater than 0.05).A total of 168 organisms were isolated from this study and they include; E. coli(48.3%), Klebsiella pnuemonaie(11.8%), Klebsiella ozanae(13.2%), Enterobacter agglomerans(11.8%), Enterobacter gergoviae(14.7%), Enterobacter aerogenes (8.8%), Citrobacter freundii(8.8%), Proteus mirabilis (5.9%), Serratia liquefaciens(10.3%) and Acinetobacter lwoffii (2.9%). E. coliwas isolated more frequently (48.3%) compared to other species and was more frequent in non-cast wells (50.0%) compared to cast wells (47.1%). Other species were more frequent in cast wells. The bacteria isolated from this study are mostly of enteric origin; these organisms acquire public health significance under certain conditions, therefore, their removal in drinking water should be given moderate priority. The wells in this study fail to meet the minimum standard for safe drinking water and are therefore not safe for domestic purposes especially for drinking purpose without adequate treatment.

scholarly journals Appraisal of Public Pipe-borne Water Quality in Jimeta/Yola Adamawa State (Nigeria): From the Treatment-plants to End-user Points

2020 ◽  
pp. 53-65
Author(s):  
I. B. Bwatanglang ◽  
E. Yonnana ◽  
Lynna D. Ibrahim ◽  
Abdulrahman I. Kubo ◽  
B. K. Elijah ◽  
...  
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Water Samples ◽  
Water Distribution ◽  
Treatment Plant ◽  
Poor Quality ◽  
End User ◽  
Point Of Use ◽  
Piped Water

This research aimed at assessing the drinking water quality of piped water distribution in Jimeta-Yola Adamawa State, Nigeria. The strategy was based on establishing the possibilities of contaminants underlying the distribution channels compromising the quality from the treatment source to the consumer point of use. Selected heavy metals and physiological parameters were determined toward establishing the water quality indices (WQI). Though, most of the parameter determined fell below or within the permissible limits (PL) set by WHO for drinking water, the results indicated significant (p<0.05) differences in the concentrations determined in the treatment plants (Yola treatment plant (YTP) and Jimeta treatment plant (JMTP)) with those at the consumer endpoints (YTPC and JMTPC). The results showed the WQI at the treatment plants being compromised due to the induction of pollutants across the distribution pipes. The water samples at the treatment point (JMTP) were excellent, having WQI <25 and good quality at YTP (25< WQI<50). However, the water quality on leaving the treatment source was observed to slightly change to poor quality at JMTPC (WQI= 57.00), and further observed to be in moderate-good quality at YTPC (WQI=49.27). Further analysis showed an increase in bacterial counts in the water samples at the consumer points.  Escherichia coli concentrations of 565 and 718 cfu /100 mL were detected in samples from YTPC and JMTPC, despite the fact the water was observed to be free from bacteria at the treatment plants.

scholarly journals DETEKSI BAKTERI Escherichia coli DALAM AIR MINUM ISI ULANG YANG DISTERILISASI ULTRAVIOLET DI WILAYAH KECAMATAN JAGAKARSA

Jurnal BIOMA ◽  
2017 ◽  
Vol 11 (1) ◽  
pp. 73
Author(s):  
Rezki Rachmawati ◽  
Muzajjanah Muzajjanah ◽  
Yoswita Rustam
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Coliform Bacterium ◽  
Probable Number ◽  
E Coli ◽  
Biological Contamination ◽  
Drinking Water Samples

Refill Water Depot is currently more widely circulated and used as an alternative drinking water supply by the public. However the still unclear about the quality of the drinking water refill generated primarily of biological content. Parameters of biological contamination in drinking water caused by the Escherichia coli and coliform bacterium. This study aims to identify E. coli and coliforms in drinking water refill. Refill drinking water samples obtained from 16 drinking water refill from Jagakarsa subdsitrict. The method used is descriptive. Refill drinking water samples was taken and tested in the MPN (Most Probable Number) method and then to be tested in identification of E. coli. The results of testing the drinking water refill obtained 15 samples positive for coliform bacteria. Samples were positive for E. coli bacteria that sample B.1 and F.2.

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