scholarly journals Atomic Resolution Structure of the Oncolytic Parvovirus LuIII by Electron Microscopy and 3D Image Reconstruction

Viruses ◽  
2017 ◽  
Vol 9 (11) ◽  
pp. 321 ◽  
Author(s):  
Nikéa Pittman ◽  
Adam Misseldine ◽  
Lorena Geilen ◽  
Sujata Halder ◽  
J. Smith ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Image Reconstruction ◽  
Atomic Resolution ◽  
3D Image ◽  
3D Image Reconstruction ◽  
Resolution Structure

scholarly journals A new solution to the curved Ewald sphere problem for 3D image reconstruction in electron microscopy

2021 ◽  
Vol 224 ◽  
pp. 113234
Author(s):  
J.P.J. Chen ◽  
K.E. Schmidt ◽  
J.C.H. Spence ◽  
R.A. Kirian
Keyword(s):  
Electron Microscopy ◽  
Image Reconstruction ◽  
3D Image ◽  
3D Image Reconstruction ◽  
Ewald Sphere

scholarly journals CryoEM structure of the antibacterial target PBP1b at 3.3 Å resolution

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Nathanael A. Caveney ◽  
Sean D. Workman ◽  
Rui Yan ◽  
Claire E. Atkinson ◽  
Zhiheng Yu ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Acid Anhydride ◽  
Atomic Resolution ◽  
Inherent Difficulty ◽  
Penicillin Binding Proteins ◽  
Antibiotic Development ◽  
Class A ◽  
Membrane Mimetic ◽  
Antibacterial Target ◽  
Resolution Structure

AbstractThe pathway for the biosynthesis of the bacterial cell wall is one of the most prolific antibiotic targets, exemplified by the widespread use of β-lactam antibiotics. Despite this, our structural understanding of class A penicillin binding proteins, which perform the last two steps in this pathway, is incomplete due to the inherent difficulty in their crystallization and the complexity of their substrates. Here, we determine the near atomic resolution structure of the 83 kDa class A PBP from Escherichia coli, PBP1b, using cryogenic electron microscopy and a styrene maleic acid anhydride membrane mimetic. PBP1b, in its apo form, is seen to exhibit a distinct conformation in comparison to Moenomycin-bound crystal structures. The work herein paves the way for the use of cryoEM in structure-guided antibiotic development for this notoriously difficult to crystalize class of proteins and their complex substrates.

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