3D image reconstruction algorithms for cryo-electron-microscopy images of virus particles

2000 ◽  
Author(s):  
Peter C. Doerschuk ◽  
John E. Johnson
Keyword(s):  
Electron Microscopy ◽  
Image Reconstruction ◽  
3D Image ◽  
Reconstruction Algorithms ◽  
Virus Particles ◽  
3D Image Reconstruction ◽  
Cryo Electron Microscopy ◽  
Image Reconstruction Algorithms ◽  
Microscopy Images

Three dimensional deblurring of transmitted-light brightfield micrographs

1993 ◽  
Vol 51 ◽  
pp. 564-565
Author(s):  
Santosh Bhattacharyya
Keyword(s):  
Image Reconstruction ◽  
Three Dimensional ◽  
Transmitted Light ◽  
Reconstruction Algorithms ◽  
3D Image Reconstruction ◽  
Structural Details ◽  
Spread Function ◽  
Early Testing ◽  
Linearized Model ◽  
Image Reconstruction Algorithms

Three dimensional microscopic structures play an important role in the understanding of various biological and physiological phenomena. Structural details of neurons, such as the density, caliber and volumes of dendrites, are important in understanding physiological and pathological functioning of nervous systems. Even so, many of the widely used stains in biology and neurophysiology are absorbing stains, such as horseradish peroxidase (HRP), and yet most of the iterative, constrained 3D optical image reconstruction research has concentrated on fluorescence microscopy. It is clear that iterative, constrained 3D image reconstruction methodologies are needed for transmitted light brightfield (TLB) imaging as well. One of the difficulties in doing so, in the past, has been in determining the point spread function of the system.We have been developing several variations of iterative, constrained image reconstruction algorithms for TLB imaging. Some of our early testing with one of them was reported previously. These algorithms are based on a linearized model of TLB imaging.

scholarly journals A new solution to the curved Ewald sphere problem for 3D image reconstruction in electron microscopy

2021 ◽  
Vol 224 ◽  
pp. 113234
Author(s):  
J.P.J. Chen ◽  
K.E. Schmidt ◽  
J.C.H. Spence ◽  
R.A. Kirian
Keyword(s):  
Electron Microscopy ◽  
Image Reconstruction ◽  
3D Image ◽  
3D Image Reconstruction ◽  
Ewald Sphere

Cryo-Electron Microscopy and Image Reconstruction of PBCV-1, an ALGAL Virus with T=169, Icosahedral Lattice Symmetry

1998 ◽  
Vol 4 (S2) ◽  
pp. 948-949
Author(s):  
X. Yan ◽  
N. H. Olson ◽  
J. L. Van Etten ◽  
T. S. Baker
Keyword(s):  
Electron Microscopy ◽  
Image Reconstruction ◽  
Virus Infectivity ◽  
Virion Protein ◽  
Virus Surface ◽  
Large Plaque ◽  
Algal Virus ◽  
3D Image Reconstruction ◽  
Cryo Electron Microscopy ◽  
Reconstruction Methods

Paramecium bursaria Chlorella virus l(PBCV-l), the type species of the Pycodnaviridae family, is a large, plaque-forming virus that replicates in certain unicellular, exsymbiotic, Chlorella-like green algae. The virion (∼lxl09 daltons) has a 330,740 base pair dsDNA genome encapsulated within an icosahedral shell of ∼190nm diameter. Purified virions contain more than 50 different proteins, which account for 65% of the total virion mass and range in size from 10 to more than 200kDa. Vp54, the major capsid protein (40% of total virion protein) is a myristilated glycoprotein but it is not phosphorylated. Three other proteins are located on the virus surface. Virions contain 5-10% lipid, located inside the glycoprotein shell, which is required for virus infectivity. Little is known about the detailed ultrastructure of PBCV-1 virions, though some information has been obtained with sectioned, negatively-stained, and metal-shadowed specimens. We have used cryo-electron microscopy and three-dimensional (3D) image reconstruction methods to examine the native morphology of PBCV-1.

scholarly journals Atomic Resolution Structure of the Oncolytic Parvovirus LuIII by Electron Microscopy and 3D Image Reconstruction

Viruses ◽  
2017 ◽  
Vol 9 (11) ◽  
pp. 321 ◽  
Author(s):  
Nikéa Pittman ◽  
Adam Misseldine ◽  
Lorena Geilen ◽  
Sujata Halder ◽  
J. Smith ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Image Reconstruction ◽  
Atomic Resolution ◽  
3D Image ◽  
3D Image Reconstruction ◽  
Resolution Structure
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