scholarly journals Quantum Dot Nanobeads Based Fluorescence Immunoassay for the Quantitative Detection of Sulfamethazine in Chicken and Milk

Sensors ◽  
2021 ◽  
Vol 21 (19) ◽  
pp. 6604
Author(s):  
Daixian Wei ◽  
Jintao Liu ◽  
Zexiang Wang ◽  
Shu Zhou ◽  
Suhua Wang ◽  
...  
Keyword(s):  
Quantum Dot ◽  
High Reliability ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Quantitative Detection ◽  
Fluorescence Immunoassay ◽  
Limits Of Detection ◽  
Milk Samples ◽  
Livestock Feed

Sulfamethazine (SMZ) as a broad antibiotic is widely used in livestock and poultry. However, the abuse of SMZ in livestock feed can lead to SMZ residues in food and the resistance of bacteria to drugs. Thus, a method for the detection of SMZ in food is urgently needed. In this study, quantum dot (QD) nanobeads (QBs) were synthesized by encapsulating CdSe/ZnS QDs using a microemulsion technique. The prepared QBs as signal probes were applied in lateral flow immunoassay (LFIA) for the detection of SMZ in chicken and milk. Our proposed method had limits of detection of 0.1138–0.0955 ng/mL and corresponding linear ranges of 0.2–12.5, 0.1–15 ng/mL in chicken and milk samples, respectively. The recovery of LFIA for the detection of SMZ was 80.9–109.4% and 84–101.6% in chicken and milk samples, respectively. Overall, the developed QBs-LFIA had high reliability and excellent potential for rapid and sensitive screening of SMZ in food.

scholarly journals Comparison of Nanosized Markers in Lateral Flow Immunoassay of Antibiotic Lincomycin

Proceedings ◽  
2020 ◽  
Vol 60 (1) ◽  
pp. 41
Author(s):  
Olga D. Hendrickson ◽  
Kseniya V. Serebrennikova ◽  
Elena A. Zvereva ◽  
Demid S. Popravko ◽  
Anatoly V. Zherdev ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Gold Nanoparticle ◽  
Limit Of Detection ◽  
Cost Effective ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Quantitative Detection ◽  
Site Testing ◽  
Limits Of Detection ◽  
Chemical Contaminants

Improving the sensitivity of the competitive lateral flow immunoassay (LFIA) is important, given the increasing demands for the monitoring of chemical contaminants in food. The choice of nanosized marker is an essential task for improving the LFIA sensitivity. In this study, a CdSe/ZnS quantum dot (QD)-based LFIA combined with a portable reader was developed for rapid and quantitative detection of an antibiotic lincomycin (LIN). The performance of the proposed fluorescence LFIA was compared to the conventional gold nanoparticle (AuNP)-based LFIA realized with the same immunoreagents. The visual cutoff values were 10 ng/mL for AuNP-based LFIA and 20 ng/mL for QD-based LFIA. Furthermore, the instrumental limits of detection have been shown to be comparable for both nanosized markers and amounted to 0.4 ng/mL for AuNPs and 0.2 ng/mL for QDs, respectively. According to the results obtained, both LFIAs may be used for rapid, cost-effective, on-site testing of antibiotics, in particular LIN. However, the QD-based LFIA exhibits lowest limit of detection with the least immunoreagent consumption, which makes it economically beneficial.

A quantum dot-based lateral flow immunoassay for the sensitive detection of human heart fatty acid binding protein (hFABP) in human serum

Talanta ◽  
2018 ◽  
Vol 178 ◽  
pp. 910-915 ◽  
Author(s):  
Mihaela Savin ◽  
Carmen-Marinela Mihailescu ◽  
Iulia Matei ◽  
Dana Stan ◽  
Carmen Aura Moldovan ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Quantum Dot ◽  
Human Serum ◽  
Human Heart ◽  
Fatty Acid Binding Protein ◽  
Lateral Flow ◽  
Sensitive Detection ◽  
Lateral Flow Immunoassay ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

SERS-Based Lateral Flow Immunoassay Strip for Ultrasensitive and Quantitative Detection of Acrosomal Protein SP10

2021 ◽  
Author(s):  
Bing Liu ◽  
Shiya Zheng ◽  
Qian Liu ◽  
Bingbing Gao ◽  
Xiangwei Zhao ◽  
...  
Keyword(s):  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Quantitative Detection

scholarly journals A multiplex chemiluminescent biosensor for type B-fumonisins and aflatoxin B1 quantitative detection in maize flour

The Analyst ◽  
2015 ◽  
Vol 140 (1) ◽  
pp. 358-365 ◽  
Author(s):  
Martina Zangheri ◽  
Fabio Di Nardo ◽  
Laura Anfossi ◽  
Cristina Giovannoli ◽  
Claudio Baggiani ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Quantitative Detection ◽  
Type B ◽  
Maize Flour

A multiplex chemiluminescence biosensor based on a lateral flow immunoassay was developed for on-site quantitative detection of fumonisins and aflatoxin B1 in maize.

scholarly journals Sensitive and Simultaneous Detection of SARS-CoV-2-Specific IgM/IgG Using Lateral Flow Immunoassay Based on Dual-Mode Quantum Dot Nanobeads

2020 ◽  
Vol 92 (23) ◽  
pp. 15542-15549
Author(s):  
Chongwen Wang ◽  
Xingsheng Yang ◽  
Bing Gu ◽  
Haifeng Liu ◽  
Zihui Zhou ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Dual Mode

Rapid lateral-flow immunoassay for the quantum dot-based detection of puerarin

2016 ◽  
Vol 81 ◽  
pp. 358-362 ◽  
Author(s):  
Huihua Qu ◽  
Yue Zhang ◽  
Baoping Qu ◽  
Hui Kong ◽  
Gaofeng Qin ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay
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