scholarly journals A multiplex chemiluminescent biosensor for type B-fumonisins and aflatoxin B1 quantitative detection in maize flour

The Analyst ◽  
2015 ◽  
Vol 140 (1) ◽  
pp. 358-365 ◽  
Author(s):  
Martina Zangheri ◽  
Fabio Di Nardo ◽  
Laura Anfossi ◽  
Cristina Giovannoli ◽  
Claudio Baggiani ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Quantitative Detection ◽  
Type B ◽  
Maize Flour

A multiplex chemiluminescence biosensor based on a lateral flow immunoassay was developed for on-site quantitative detection of fumonisins and aflatoxin B1 in maize.

scholarly journals A Rapid and Sensitive Fluorescent Microsphere-Based Lateral Flow Immunoassay for Determination of Aflatoxin B1 in Distillers’ Grains

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2109
Author(s):  
Zifei Wang ◽  
Pengjie Luo ◽  
Baodong Zheng
Keyword(s):  
Aflatoxin B1 ◽  
High Performance ◽  
Animal Feed ◽  
Limit Of Detection ◽  
Lateral Flow ◽  
Distillers Grains ◽  
Detection Methods ◽  
Lateral Flow Immunoassay ◽  
Quantitative Detection ◽  
Fluorescent Microsphere

Aflatoxin B1 (AFB1) is a toxic compound naturally produced by the genera Aspergillus. Distillers’ grains can be used as animal feed since they have high content of crude protein and other nutrients. However, they are easily contaminated by mycotoxins, and currently there are no rapid detection methods for AFB1 in distillers’ grains. In this study, a lateral flow immunoassay (LFIA) based on red fluorescent microsphere (FM), is developed for quantitative detection of AFB1 in distillers’ grains. The whole test can be completed within 15 min, with the cut-off value being 25.0 μg/kg, and the quantitative limit of detection (qLOD) being 3.4 μg/kg. This method represents satisfactory recoveries of 95.2–113.0%, and the coefficients of variation (CVs) are less than 7.0%. Furthermore, this technique is successfully used to analyze AFB1 in real samples, and the results indicates good consistency with that of high-performance liquid chromatography (HPLC). The correlation coefficient is found to be greater than 0.99. The proposed test strip facilitates on-site, cost-effective, and sensitive monitoring of AFB1 in distillers’ grains.

Colour-encoded lateral flow immunoassay for the simultaneous detection of aflatoxin B1 and type-B fumonisins in a single Test line

Talanta ◽  
2019 ◽  
Vol 192 ◽  
pp. 288-294 ◽  
Author(s):  
Fabio Di Nardo ◽  
Eugenio Alladio ◽  
Claudio Baggiani ◽  
Simone Cavalera ◽  
Cristina Giovannoli ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Test Line ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Type B ◽  
Single Test

SERS-Based Lateral Flow Immunoassay Strip for Ultrasensitive and Quantitative Detection of Acrosomal Protein SP10

2021 ◽  
Author(s):  
Bing Liu ◽  
Shiya Zheng ◽  
Qian Liu ◽  
Bingbing Gao ◽  
Xiangwei Zhao ◽  
...  
Keyword(s):  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Quantitative Detection

Upconverting nanoparticle clustering based rapid quantitative detection of tetrahydrocannabinol (THC) on lateral-flow immunoassay

The Analyst ◽  
2020 ◽  
Author(s):  
Rohit Chand ◽  
Neha Mittal ◽  
Seshasai Srinivasan ◽  
Amin Reza Rajabzadeh
Keyword(s):  
Lateral Flow ◽  
Psychoactive Drug ◽  
Lateral Flow Immunoassay ◽  
Quantitative Detection

Cannabis, also known as marijuana, is the most abused psychoactive drug worldwide.

scholarly journals Immunochromatographic Tests for Mycotoxins Detection with the Use of Ultrasmall Magnetite Nanoparticles

2021 ◽  
Vol 2 (1) ◽  
pp. 100
Author(s):  
Alina V. Petrakova ◽  
Alexandr E. Urusov ◽  
Anatoly V. Zherdev ◽  
Boris B. Dzantiev
Keyword(s):  
Aflatoxin B1 ◽  
Magnetite Nanoparticles ◽  
Fe3o4 Nanoparticles ◽  
Raw Materials ◽  
Total Surface Area ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Control Food ◽  
Lateral Flow Tests ◽  
T2 Toxin

The use of small (with a diameter of 7–12 nm) superparamagnetic Fe3O4 nanoparticles as carriers for antibodies in lateral flow immunoassay is considered. Increased total surface area for such suspension provides a concentration of analytes with an increase in their concentration up to 50 times. When the concentrated complexes were redissolved, aggregates with diameters of 100–500 nm were obtained, serving as colored markers in the assay. Further, magnetite–antibodies complexes are more tolerant to methanol (up to 30%) than native antibodies, thus providing minimal dilution of tested extracts. Lateral flow tests for mycotoxins zearalenone, T2-toxin, and aflatoxin B1 were developed and demonstrated applicability to control food products and raw materials.

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