scholarly journals Sensitive and Simultaneous Detection of SARS-CoV-2-Specific IgM/IgG Using Lateral Flow Immunoassay Based on Dual-Mode Quantum Dot Nanobeads

2020 ◽  
Vol 92 (23) ◽  
pp. 15542-15549
Author(s):  
Chongwen Wang ◽  
Xingsheng Yang ◽  
Bing Gu ◽  
Haifeng Liu ◽  
Zihui Zhou ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Dual Mode

scholarly journals Ultrasensitive and Simultaneous Detection of Two Specific SARS-CoV-2 Antigens in Human Specimens Using Direct/Enrichment Dual-Mode Fluorescence Lateral Flow Immunoassay

2021 ◽  
Vol 13 (34) ◽  
pp. 40342-40353
Author(s):  
Chongwen Wang ◽  
Xiaodan Cheng ◽  
Liyan Liu ◽  
Xiaochang Zhang ◽  
Xingsheng Yang ◽  
...  
Keyword(s):  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Dual Mode

A quantum dot-based lateral flow immunoassay for the sensitive detection of human heart fatty acid binding protein (hFABP) in human serum

Talanta ◽  
2018 ◽  
Vol 178 ◽  
pp. 910-915 ◽  
Author(s):  
Mihaela Savin ◽  
Carmen-Marinela Mihailescu ◽  
Iulia Matei ◽  
Dana Stan ◽  
Carmen Aura Moldovan ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Quantum Dot ◽  
Human Serum ◽  
Human Heart ◽  
Fatty Acid Binding Protein ◽  
Lateral Flow ◽  
Sensitive Detection ◽  
Lateral Flow Immunoassay ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

scholarly journals The simultaneous detection of the squamous cell carcinoma antigen and cancer antigen 125 in the cervical cancer serum using nano-Ag polydopamine nanospheres in an SERS-based lateral flow immunoassay

RSC Advances ◽  
2020 ◽  
Vol 10 (49) ◽  
pp. 29156-29170
Author(s):  
Ji Xia ◽  
Yifan Liu ◽  
Menglin Ran ◽  
Wenbo Lu ◽  
Liyan Bi ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Squamous Cell Carcinoma Antigen ◽  
Cancer Antigen 125 ◽  
Cancer Antigen

Based on SERS-based lateral flow immunoassay, nano-Ag polydopamine nanospheres was used for detecting squamous cell carcinoma antigen and cancer antigen 125 simultaneously in cervical cancer serum.

Rapid lateral-flow immunoassay for the quantum dot-based detection of puerarin

2016 ◽  
Vol 81 ◽  
pp. 358-362 ◽  
Author(s):  
Huihua Qu ◽  
Yue Zhang ◽  
Baoping Qu ◽  
Hui Kong ◽  
Gaofeng Qin ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay

scholarly journals Noncompetitive Chromogenic Lateral-Flow Immunoassay for Simultaneous Detection of Microcystins and Nodularin

Biosensors ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 79 ◽  
Author(s):  
Sultana Akter ◽  
Teemu Kustila ◽  
Janne Leivo ◽  
Gangatharan Muralitharan ◽  
Markus Vehniäinen ◽  
...  
Keyword(s):  
Water Quality ◽  
Simultaneous Detection ◽  
Antibody Fragment ◽  
Water Quality Monitoring ◽  
Lateral Flow ◽  
Cyanobacterial Blooms ◽  
World Health ◽  
Lateral Flow Immunoassay ◽  
Guideline Value ◽  
Health Organization

Cyanobacterial blooms cause local and global health issues by contaminating surface waters. Microcystins and nodularins are cyclic cyanobacterial peptide toxins comprising numerous natural variants. Most of them are potent hepatotoxins, tumor promoters, and at least microcystin-LR is possibly carcinogenic. In drinking water, the World Health Organization (WHO) recommended the provisional guideline value of 1 µg/L for microcystin-LR. For water used for recreational activity, the guidance values for microcystin concentration varies mostly between 4–25 µg/L in different countries. Current immunoassays or lateral flow strips for microcystin/nodularin are based on indirect competitive method, which are generally more prone to sample interference and sometimes hard to interpret compared to two-site immunoassays. Simple, sensitive, and easy to interpret user-friendly methods for first line screening of microcystin/nodularin near water sources are needed for assessment of water quality and safety. We describe the development of a two-site sandwich format lateral-flow assay for the rapid detection of microcystins and nodularin-R. A unique antibody fragment capable of broadly recognizing immunocomplexes consisting of a capture antibody bound to microcystins/nodularin-R was used to develop the simple lateral flow immunoassay. The assay can visually detect the major hepatotoxins (microcystin-LR, -dmLR, -RR, -dmRR, -YR, -LY, -LF -LW, and nodularin-R) at and below the concentration of 4 µg/L. The signal is directly proportional to the concentration of the respective toxin, and the use of alkaline phosphatase activity offers a cost efficient alternative by eliminating the need of toxin conjugates or other labeling system. The easy to interpret assay has the potential to serve as a microcystins/nodularin screening tool for those involved in water quality monitoring such as municipal authorities, researchers, as well as general public concerned of bathing water quality.

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