scholarly journals Microcapsule-Based Signal Amplification Method for Biomolecules

Sensors ◽  
2019 ◽  
Vol 19 (12) ◽  
pp. 2711
Author(s):  
Masaki Yamaguchi
Keyword(s):  
Optical Density ◽  
Signal Amplification ◽  
Irradiation Time ◽  
Effective Means ◽  
Layer By Layer ◽  
Direct Signal ◽  
Amplification Method ◽  
Optical Density Change ◽  
Target Molecules ◽  
Detection Of Biomolecules

The direct signal amplification of target molecules could be an effective means of increasing the sensitivity and reducing the size of biosensors. The purpose of this study was to propose a novel signal amplification method suitable for the detection of biomolecules using microcapsules that can quickly respond to concentration variation. This microcapsule-based amplification method consists of two elements—microcapsules and a well-array. The microcapsules consist of (i) an inner shell fabricated through layer-by-layer assembly, (ii) a lipid bilayer, and (iii) loaded target molecules. In this method, the inner surface of the well-array was modified using TiO2 as a photocatalyst. The diameter and thickness of the fabricated micro-capsules for biomarker loading were shown to be 2.7 μm and 78 nm, respectively. An ultraviolet (UV) irradiation time of 5 min was needed when the change in optical density reached 90% saturation of the optical density change. Dye molecules were incorporated into the microcapsules and were subsequently released, and the concentration of the released solution changed in proportion with the encapsulated dye concentration. This demonstrates the proof of concept for this novel signal amplification method based on microcapsules.

Carbon nanoparticle-protected aptamers for highly sensitive and selective detection of biomolecules based on nuclease-assisted target recycling signal amplification

2014 ◽  
Vol 50 (57) ◽  
pp. 7646-7648 ◽  
Author(s):  
Xiaoyan Lin ◽  
Liang Cui ◽  
Yishun Huang ◽  
Ya Lin ◽  
Yi Xie ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Carbon Nanoparticles ◽  
Sensitive Detection ◽  
Selective Detection ◽  
Carbon Nanoparticle ◽  
Target Recycling ◽  
Amplification Method ◽  
Highly Sensitive ◽  
Detection Of Biomolecules ◽  
Highly Sensitive Detection

A nuclease-assisted target recycling signal amplification method based on carbon nanoparticles for highly sensitive detection of biomolecules was developed.

scholarly journals SARS-CoV-2 RNA Detection with Duplex-Specific Nuclease Signal Amplification

Micromachines ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 197
Author(s):  
Meiqing Liu ◽  
Haoran Li ◽  
Yanwei Jia ◽  
Pui-In Mak ◽  
Rui P. Martins
Keyword(s):  
Signal Amplification ◽  
Incubation Temperature ◽  
Dna Probes ◽  
Detection Sensitivity ◽  
N Gene ◽  
Rna Detection ◽  
Gold Standard Method ◽  
Complementary Method ◽  
Amplification Method ◽  
Virus Rna

The emergence of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a zoonotic pathogen, has led to the outbreak of coronavirus disease 2019 (COVID-19) pandemic and brought serious threats to public health worldwide. The gold standard method for SARS-CoV-2 detection requires both reverse transcription (RT) of the virus RNA to cDNA and then polymerase chain reaction (PCR) for the cDNA amplification, which involves multiple enzymes, multiple reactions and a complicated assay optimization process. Here, we developed a duplex-specific nuclease (DSN)-based signal amplification method for SARS-CoV-2 detection directly from the virus RNA utilizing two specific DNA probes. These specific DNA probes can hybridize to the target RNA at different locations in the nucleocapsid protein gene (N gene) of SARS-CoV-2 to form a DNA/RNA heteroduplex. DSN cleaves the DNA probe to release fluorescence, while leaving the RNA strand intact to be bound to another available probe molecule for further cleavage and fluorescent signal amplification. The optimized DSN amount, incubation temperature and incubation time were investigated in this work. Proof-of-principle SARS-CoV-2 detection was demonstrated with a detection sensitivity of 500 pM virus RNA. This simple, rapid, and direct RNA detection method is expected to provide a complementary method for the detection of viruses mutated at the PCR primer-binding regions for a more precise detection.

Layer by layer assembly of biotinylated protein networks for signal amplification

2013 ◽  
Vol 49 (24) ◽  
pp. 2397 ◽  
Author(s):  
Yu W. Chu ◽  
Bo Y. Wang ◽  
Huei-Shian Lin ◽  
Tai-Yen Lin ◽  
Yuan-Jen Hung ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Protein Networks ◽  
Layer By Layer ◽  
Layer By Layer Assembly ◽  
Layer Assembly

Mass Spectrometry Signal Amplification Method for Attomolar Detection of Antigens Using Small-Molecule-Tagged Gold Microparticles

2008 ◽  
Vol 120 (49) ◽  
pp. 9660-9663 ◽  
Author(s):  
Jung Rok Lee ◽  
Juhee Lee ◽  
Sang Kyung Kim ◽  
Kwang Pyo Kim ◽  
Hyung Soon Park ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Small Molecule ◽  
Signal Amplification ◽  
Amplification Method

scholarly journals Highly sensitive and multiplexed in situ protein profiling with cleavable fluorescent streptavidin

2019 ◽  
Author(s):  
Renjie Liao ◽  
Diego Mastroeni ◽  
Paul D. Coleman ◽  
Jia Guo
Keyword(s):  
Signal Amplification ◽  
Individual Cell ◽  
Protein Detection ◽  
Protein Profiling ◽  
Detection Sensitivity ◽  
Layer By Layer ◽  
Protein Targets ◽  
Highly Sensitive ◽  
Wide Range

AbstractThe ability to perform highly sensitive and multiplexed in situ protein analysis is crucial to advance our understanding of normal physiology and disease pathogenesis. To achieve this goal, here we develop an approach using cleavable biotin conjugated antibodies and cleavable fluorescent streptavidin (CFS). In this approach, protein targets are first recognized by the cleavable biotin labeled antibodies. Subsequently, CFS is applied to stain the protein targets. Though layer-by-layer signal amplification using cleavable biotin conjugated orthogonal antibodies and CSF, the protein detection sensitivity can be enhanced by at least 10 fold, compared with the existing methods. After imaging, the fluorophores and the biotins unbound to streptavidin are removed by chemical cleavage. The leftover streptavidin is blocked by biotin. Upon reiterative analysis cycles, a large number of different proteins with a wide range of expression levels can be unambiguously detected in individual cell in situ.

scholarly journals Signal amplification method for miR-205 assay through combining graphene oxide with duplex-specific nuclease

RSC Advances ◽  
2019 ◽  
Vol 9 (47) ◽  
pp. 27341-27346 ◽  
Author(s):  
Zhaoqi Yang ◽  
Lan Qin ◽  
Dutao Yang ◽  
Weixia Chen ◽  
Yue Qian ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Nasopharyngeal Carcinoma ◽  
Signal Amplification ◽  
Amplification Method ◽  
Radiation Resistant

A signal amplified method for detecting a biomarker of radiation-resistant nasopharyngeal carcinoma using graphene oxide and duplex-specific nuclease was constructed.

A dual signal amplification method for exosome detection based on DNA dendrimer self-assembly

The Analyst ◽  
2019 ◽  
Vol 144 (6) ◽  
pp. 1995-2002 ◽  
Author(s):  
Mei-Ling Gao ◽  
Fang He ◽  
Bin-Cheng Yin ◽  
Bang-Ce Ye
Keyword(s):  
Self Assembly ◽  
Signal Amplification ◽  
Vital Role ◽  
Amplification Method ◽  
Dual Signal Amplification

An increasing number of studies have found that circulating exosomes play a vital role in the occurrence and metastasis of cancer.

Sign in / Sign up

Export Citation Format

Share Document