scholarly journals Signal amplification method for miR-205 assay through combining graphene oxide with duplex-specific nuclease

RSC Advances ◽  
2019 ◽  
Vol 9 (47) ◽  
pp. 27341-27346 ◽  
Author(s):  
Zhaoqi Yang ◽  
Lan Qin ◽  
Dutao Yang ◽  
Weixia Chen ◽  
Yue Qian ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Nasopharyngeal Carcinoma ◽  
Signal Amplification ◽  
Amplification Method ◽  
Radiation Resistant

A signal amplified method for detecting a biomarker of radiation-resistant nasopharyngeal carcinoma using graphene oxide and duplex-specific nuclease was constructed.

scholarly journals A graphene oxide/gold nanoparticle-based amplification method for SERS immunoassay of cardiac troponin I

The Analyst ◽  
2019 ◽  
Vol 144 (5) ◽  
pp. 1582-1589 ◽  
Author(s):  
Xiuli Fu ◽  
Yunqing Wang ◽  
Yongming Liu ◽  
Huitao Liu ◽  
Longwen Fu ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Gold Nanoparticle ◽  
Cardiac Troponin ◽  
Signal Amplification ◽  
Troponin I ◽  
Cardiac Troponin I ◽  
Sensitive Detection ◽  
Multiple Signal ◽  
Amplification Method

A multiple signal amplification of a SERS biosensor was developed for sensitive detection of cTnI with the aid of GO/AuNP complexes.

A sensitive dual signal amplification method for western blotting based on antibody-functionalised graphene oxide and gold nanoparticles

The Analyst ◽  
2012 ◽  
Vol 137 (16) ◽  
pp. 3620 ◽  
Author(s):  
Hongjun Lin ◽  
Jingrui Huo ◽  
Aihong Zhang ◽  
Yingfu Liu ◽  
Qingming Wang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Graphene Oxide ◽  
Western Blotting ◽  
Signal Amplification ◽  
Amplification Method ◽  
Dual Signal Amplification

scholarly journals Cancer-associated fibroblasts promote the survival of irradiated nasopharyngeal carcinoma cells via the NF-κB pathway

2021 ◽  
Vol 40 (1) ◽  
Author(s):  
Weiqiang Huang ◽  
Longshan Zhang ◽  
Mi Yang ◽  
Xixi Wu ◽  
Xiaoqing Wang ◽  
...  
Keyword(s):  
Dna Damage ◽  
Nasopharyngeal Carcinoma ◽  
Enrichment Analysis ◽  
Underlying Mechanism ◽  
Gene Set Enrichment Analysis ◽  
Cancer Associated Fibroblasts ◽  
Western Blot Assay ◽  
Damage Level ◽  
Radiation Resistant

Abstract Background Irradiation has emerged as a valid tool for nasopharyngeal carcinoma (NPC) in situ treatment; however, NPC derived from tissues treated with irradiation is a main cause cancer-related death. The purpose of this study is to uncover the underlying mechanism regarding tumor growth after irradiation and provided potential therapeutic strategy. Methods Fibroblasts were extracted from fresh NPC tissue and normal nasopharyngeal mucosa. Immunohistochemistry was conducted to measure the expression of α-SMA and FAP. Cytokines were detected by protein array chip and identified by real-time PCR. CCK-8 assay was used to detect cell proliferation. Radiation-resistant (IRR) 5-8F cell line was established and colony assay was performed to evaluate tumor cell growth after irradiation. Signaling pathways were acquired via gene set enrichment analysis (GSEA). Comet assay and γ-H2AX foci assay were used to measure DNA damage level. Protein expression was detected by western blot assay. In vivo experiment was performed subcutaneously. Results We found that radiation-resistant NPC tissues were constantly infiltrated with a greater number of cancer-associated fibroblasts (CAFs) compared to radiosensitive NPC tissues. Further research revealed that CAFs induced the formation of radioresistance and promoted NPC cell survival following irradiation via the IL-8/NF-κB pathway to reduce irradiation-induced DNA damage. Treatment with Tranilast, a CAF inhibitor, restricted the survival of CAF-induced NPC cells and attenuated the of radioresistance properties. Conclusions Together, these data demonstrate that CAFs can promote the survival of irradiated NPC cells via the NF-κB pathway and induce radioresistance that can be interrupted by Tranilast, suggesting the potential value of Tranilast in sensitizing NPC cells to irradiation.

scholarly journals SARS-CoV-2 RNA Detection with Duplex-Specific Nuclease Signal Amplification

Micromachines ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 197
Author(s):  
Meiqing Liu ◽  
Haoran Li ◽  
Yanwei Jia ◽  
Pui-In Mak ◽  
Rui P. Martins
Keyword(s):  
Signal Amplification ◽  
Incubation Temperature ◽  
Dna Probes ◽  
Detection Sensitivity ◽  
N Gene ◽  
Rna Detection ◽  
Gold Standard Method ◽  
Complementary Method ◽  
Amplification Method ◽  
Virus Rna

The emergence of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a zoonotic pathogen, has led to the outbreak of coronavirus disease 2019 (COVID-19) pandemic and brought serious threats to public health worldwide. The gold standard method for SARS-CoV-2 detection requires both reverse transcription (RT) of the virus RNA to cDNA and then polymerase chain reaction (PCR) for the cDNA amplification, which involves multiple enzymes, multiple reactions and a complicated assay optimization process. Here, we developed a duplex-specific nuclease (DSN)-based signal amplification method for SARS-CoV-2 detection directly from the virus RNA utilizing two specific DNA probes. These specific DNA probes can hybridize to the target RNA at different locations in the nucleocapsid protein gene (N gene) of SARS-CoV-2 to form a DNA/RNA heteroduplex. DSN cleaves the DNA probe to release fluorescence, while leaving the RNA strand intact to be bound to another available probe molecule for further cleavage and fluorescent signal amplification. The optimized DSN amount, incubation temperature and incubation time were investigated in this work. Proof-of-principle SARS-CoV-2 detection was demonstrated with a detection sensitivity of 500 pM virus RNA. This simple, rapid, and direct RNA detection method is expected to provide a complementary method for the detection of viruses mutated at the PCR primer-binding regions for a more precise detection.

Mass Spectrometry Signal Amplification Method for Attomolar Detection of Antigens Using Small-Molecule-Tagged Gold Microparticles

2008 ◽  
Vol 120 (49) ◽  
pp. 9660-9663 ◽  
Author(s):  
Jung Rok Lee ◽  
Juhee Lee ◽  
Sang Kyung Kim ◽  
Kwang Pyo Kim ◽  
Hyung Soon Park ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Small Molecule ◽  
Signal Amplification ◽  
Amplification Method

Frontispiece: Photosensitization of Molecular Oxygen on Graphene Oxide for Ultrasensitive Signal Amplification

2018 ◽  
Vol 24 (11) ◽  
Author(s):  
Xinfeng Zhang ◽  
Li Deng ◽  
Chengpeng Huang ◽  
Jinyi Zhang ◽  
Xiandeng Hou ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Molecular Oxygen ◽  
Signal Amplification
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