scholarly journals Label Free Detection of CD4+ and CD8+ T Cells Using the Optofluidic Ring Resonator

Sensors ◽  
2010 ◽  
Vol 10 (6) ◽  
pp. 5798-5808 ◽  
Author(s):  
John T. Gohring ◽  
Xudong Fan
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Ring Resonator ◽  
Label Free ◽  
Label Free Detection

scholarly journals Label-free detection of morpholino-DNA hybridization using a silicon photonics suspended slab micro-ring resonator

2021 ◽  
pp. 1-1
Author(s):  
Soha Yousuf ◽  
Jongmin Kim ◽  
Ajymurat Orozaliev ◽  
Marcus Dahlem ◽  
Yong-Ak Song ◽  
...  
Keyword(s):  
Silicon Photonics ◽  
Dna Hybridization ◽  
Ring Resonator ◽  
Label Free ◽  
Label Free Detection

Two-Dimensional Label-Free Affinity Analysis of Tumor-Specific CD8 T Cells with a Biomimetic Plasmonic Sensor

ACS Sensors ◽  
2018 ◽  
Vol 3 (11) ◽  
pp. 2286-2295 ◽  
Author(s):  
Maria Soler ◽  
Xiaokang Li ◽  
Aurelian John-Herpin ◽  
Julien Schmidt ◽  
George Coukos ◽  
...  
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Two Dimensional ◽  
Label Free ◽  
Plasmonic Sensor

scholarly journals Photonic Biosensor for Label-Free Detection Based on Photonic Nanostructures on Si-Waveguide Ring Resonator

2021 ◽  
Vol 6 (1) ◽  
pp. 39
Author(s):  
Hoang Anh Truong ◽  
Yugang Shang ◽  
Shunsuke Abe ◽  
Nobuyuki Matsuda ◽  
Hirohito Yamada
Keyword(s):  
Quality Factor ◽  
Ring Resonator ◽  
Clinical Diagnostics ◽  
Label Free ◽  
Electromagnetic Simulations ◽  
Label Free Detection ◽  
Matter Interaction ◽  
Light Matter Interaction ◽  
Conventional Structure ◽  
Waveguide Ring

A new structure of a micro-ring resonator for label-free biosensing is proposed. The structure includes sidewall-grating Si waveguide and periodical side-blocks that can enhance the light–matter interaction. From the electromagnetic simulations, the proposed structure exhibits a four-fold improvement in terms of sensitivity compared with the conventional structure. Moreover, the quality factor of the proposed structure is not degraded from that of the conventional structure. The improved sensitivity is promising for the detection of nanoparticles that can be applied to the environmental field and clinical diagnostics.

A planar split-ring resonator-based microwave biosensor for label-free detection of biomolecules

2012 ◽  
Vol 169 ◽  
pp. 26-31 ◽  
Author(s):  
Hee-Jo Lee ◽  
Jung-Hyun Lee ◽  
Hui-Sung Moon ◽  
Ik-Soon Jang ◽  
Jong-Soon Choi ◽  
...  
Keyword(s):  
Ring Resonator ◽  
Split Ring Resonator ◽  
Label Free ◽  
Split Ring ◽  
Label Free Detection ◽  
Detection Of Biomolecules

522 Metabolic requisites for T cell protein translation in tumors

2020 ◽  
Vol 8 (Suppl 3) ◽  
pp. A558-A558
Author(s):  
Katie Hurst ◽  
Megan Tennant ◽  
Alex Andrews ◽  
Lee Leddy ◽  
David Neskey ◽  
...  
Keyword(s):  
T Cells ◽  
Protein Synthesis ◽  
Glucose Metabolism ◽  
Solid Tumors ◽  
Cd8 T Cells ◽  
Antitumor Immunity ◽  
Protein Translation ◽  
Cell Protein ◽  
Label Free

BackgroundT cells are a secretory immune subset with the capacity to control solid tumors. Protein translation is of paramount importance in CD8 T cells, controlling proliferation, stimulation and lineage fate.MethodsHerein, we used both the fluorescent analogue of methionine homopropargylglycine (HPG) incorporation assay and O-propargyl-puromycin (OPP) method which enters the A-site of the ribosome and effectively labels and terminates nascent polypeptide chains to monitor protein synthesis in mouse and human tumors. Moreover, we employed label free quantitative proteomics (LFQ), lipidomics, metabolic analysis, and in vivo animal modeling to elucidate mechanisms of protein translation in antitumor immunity.ResultsWe found that canonical protein synthesis is restricted in endogenous CD8 tumor infiltrating lymphocytes (TILs) by the tumor microenvironment (TME). Proteomic analysis revealed that gluconeogenesis and B-oxidation of fatty acids (FAO) were upregulated in CD8 T cells under tumor stress but these metabolic sources were unable to support translation in the TME. Further, we discovered that glucose metabolism and mammalian target of rapamycin complex 1 (mTORC1) preferentially hinder protein synthesis in CD8 TILs. These data enabled the discovery that proteasomal protein degradation is the optimal source to fuel protein translation in T cells in the stress of solid tumors. We demonstrate that Rapamycin-primed T cells are preferentially powered by proteasomal proteolysis and are able to sustain protein translation in tumors and control tumor growth.ConclusionsOur data establish that canonical protein translation governed by mTORC1 and glucose metabolism is subject to inhibition in the TME and promotion of protein catabolism is a new strategy to support antitumor immunity.Ethics ApprovalAll animal experiments were in accordance with the MUSC Institutional Animal Care and Use Committee (IACUC), protocol # IACUC-2018-00422 and # IACUC-2018-00347

Simulation on biomarker sensor miniaturization based on metamaterial

2019 ◽  
Vol 33 (11) ◽  
pp. 1950135
Author(s):  
Ruitao Liu ◽  
Tianhong Cui
Keyword(s):  
Computer Simulation ◽  
Resonance Frequency ◽  
Ring Resonator ◽  
Split Ring Resonator ◽  
Important Application ◽  
Q Factor ◽  
Label Free ◽  
Split Ring ◽  
Label Free Detection ◽  
Simulation Results

Cancer is always the greatest threat to human health. In this work, a split-ring resonator (SRR)-based sensor was simulated for label-free detection on biomarkers in microwave. The model of biosensor was simulated by Computer Simulation Technology 2017 (CST2017) and some influences of feature parameters on resonance frequency and Q-factor were also discussed. From the simulation results, the model of sensor in our work showed ultrahigh Q-factor and sensitivity under different permittivities and also exhibited a trend of better performance when it works in higher frequency. Therefore, the SRR-based biosensor will become an important application in cancer precaution and treatment in the future.

Author response for "4‐1BB costimulation promotes bystander activation of human CD8 T cells"

2020 ◽  
Author(s):  
Manuel Reithofer ◽  
Sandra Rosskopf ◽  
Judith Leitner ◽  
Claire Battin ◽  
Barbara Bohle ◽  
...  
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Author Response ◽  
Bystander Activation
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