Rapid and Label-Free Detection of Breast Cancer Biomarker CA15-3 in Clinical Human Serum Samples with Optofluidic Ring Resonator Sensors

2009 ◽  
Vol 81 (24) ◽  
pp. 9858-9865 ◽  
Author(s):  
Hongying Zhu ◽  
Paul S. Dale ◽  
Charles W. Caldwell ◽  
Xudong Fan
Keyword(s):  
Breast Cancer ◽  
Human Serum ◽  
Ring Resonator ◽  
Cancer Biomarker ◽  
Label Free ◽  
Serum Samples ◽  
Human Serum Samples ◽  
Label Free Detection

scholarly journals Immunogenicity Assessment of Pegfilgrastim in Patients with Breast Cancer

2020 ◽  
Vol 9 (2) ◽  
pp. 140-144
Author(s):  
Yu. V. Medvedev ◽  
M. A. Kolganova ◽  
O. A. Sas ◽  
T. N. Komarov ◽  
E. N. Fisher ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Human Serum ◽  
Fungal Infections ◽  
Randomized Study ◽  
Elisa Method ◽  
Serum Samples ◽  
Human Serum Samples ◽  
Immunogenicity Assessment ◽  
Confirmatory Assay ◽  
Colonystimulating Factor

Introduction. Neutropenia, which is an abnormally low concentration of neutrophils in the blood, is one of the common side effects in patients receiving radio- or chemotherapy. Neutropenia usually leads to higher risks of severe bacterial and fungal infections. Such medicines as colonystimulating factor filgrastim (and its conjugates) are used to prevent and treat neutropenia in oncology patients. Immunogenicity is a potential concern for any biological product, thus, its assessment is one of the most critical necessities during the development and registration of such products.Aim. The main aim of this study was to validate the ELISA method for anti-pegfilgrastim antibodies detection in human serum samples and to apply the validated method to pegfilgrastim drugs immunogenicity assessment.Materials and methods. To assess pegfilgrastim immunogenicity, the commercial ELISA kit «PEGylated Filgrastim (Neulasta®) ADA ELISA» was used for screening, confirmatory and titer assay. Moreover, to confirm the chosen commercial kit suits the study aims it was revalidated. The absorbance values were obtained using plate immunoassay analyzer Stat Fax 3200, plate washing was performed using an automatic twochannel plate washer.Results and discussion. The ELISA method for anti-pegfilgrastim antibodies determination in human serum samples was validated and applied to the analytical part of the comparative, multicenter, blind, randomized study of pegfilgrastim efficacy and safety in patients with breast cancer, receiving myelosuppressive chemotherapy. Human serum samples were first screened for anti-drug antibodies, then «screening positive» samples were analyzed in confirmatory assay with % inhibition calculation for each sample. The «confirmed positive» samples were further characterized in titer assay.Conclusions. The ELISA method for anti-pegfilgrastim antibodies determination in human serum samples was successfully validated and applied for pegfilgrastim drugs immunogenicity assessment.

A DNAzyme-based label-free fluorescent probe for guanosine-5′-triphosphate detection

The Analyst ◽  
2020 ◽  
Vol 145 (21) ◽  
pp. 6948-6954
Author(s):  
Chengzhen Hu ◽  
Kemei Jiang ◽  
Zihao Shao ◽  
Minqing Shi ◽  
Hong-Min Meng
Keyword(s):  
Human Serum ◽  
Fluorescent Probe ◽  
Label Free ◽  
Serum Samples ◽  
Human Serum Samples

A DNAzyme-based fluorescent probe with self-phosphorylation ability for label-free and sensitive GTP detection in buffer and human serum samples.

scholarly journals Single-step label-free nanowell immunoassay accurately quantifies serum stress hormones within minutes

2021 ◽  
Vol 7 (27) ◽  
pp. eabf4401
Author(s):  
S. Reza Mahmoodi ◽  
Pengfei Xie ◽  
Daniel P. Zachs ◽  
Erik J. Peterson ◽  
Rachel S. Graham ◽  
...  
Keyword(s):  
Human Serum ◽  
Real Time ◽  
Limit Of Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Label Free ◽  
Serum Samples ◽  
Electrode Gap ◽  
Buffer Solution ◽  
Human Serum Samples ◽  
Free Cortisol

A non-faradaic label-free cortisol sensing platform is presented using a nanowell array design, in which the two probe electrodes are integrated within the nanowell structure. Rapid and low volume (≤5 μl) sensing was realized through functionalizing nanoscale volume wells with antibodies and monitoring the real-time binding events. A 28-well plate biochip was built on a glass substrate by sequential deposition, patterning, and etching steps to create a stack nanowell array sensor with an electrode gap of 40 nm. Sensor response for cortisol concentrations between 1 and 15 μg/dl in buffer solution was recorded, and a limit of detection of 0.5 μg/dl was achieved. Last, 65 human serum samples were collected to compare the response from human serum samples with results from the standard enzyme-linked immunosorbent assay (ELISA). These results confirm that nanowell array sensors could be a promising platform for point-of-care testing, where real-time, laboratory-quality diagnostic results are essential.

Simultaneous Monitoring of Febuxostat and Uric Acid in Human Serum Samples Using the Direct Square-Wave Voltammetric Method

2019 ◽  
Vol 15 (6) ◽  
pp. 678-684
Author(s):  
Biljana Nigović ◽  
Jakov Vlak
Keyword(s):  
Uric Acid ◽  
Human Serum ◽  
Oxidase Inhibitor ◽  
Serum Samples ◽  
Boron Doped Diamond ◽  
Fast Analysis ◽  
Xanthine Oxidase Inhibitor ◽  
Voltammetric Method ◽  
Square Wave ◽  
Human Serum Samples

Background: High uric acid serum level, hyperuricemia, is now associated with many diseases such as gout, chronic kidney disease, hypertension, coronary artery disease and diabetes. Febuxostat is a novel selective xanthine oxidase inhibitor approved for the treatment of hyperuricemia. Objective: The aim of this study was to develop a first analytical method for the simultaneous determination of febuxostat and uric acid. Methods: An unmodified boron-doped diamond electrode provided concurrent quantitation of drug at low levels and uric acid, which has clinical significance in the diagnosis and therapy of hyperuricemia, at relatively high concentrations. The direct square-wave voltammetric method was applied to the analysis of both analytes in human serum samples. Results: Under the optimized conditions, the linear response of peak current on febuxostat concentration was achieved in the range from 7.5 × 10-7 to 3 × 10-5 M, while uric acid showed two linear ranges of 5 × 10-6 - 5 × 10-5 M and 5 × 10-5 - 2 × 10-4 M. The method was successfully utilised for quantification of both analytes in human serum samples. Good recoveries were obtained without interference from common inorganic cations and anions as well as glucose, dopamine, ascorbic and folic acids at concentrations expected in physiological conditions. Conclusion: The great benefits of developed method are fast analysis (only 7.5 s for run), low cost and simplicity of performance.

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