scholarly journals Sulfatide-Rich Liposome Uptake by a Human-Derived Neuroblastoma Cell Line

Processes ◽  
2020 ◽  
Vol 8 (12) ◽  
pp. 1615
Author(s):  
Daniel Arroyo-Ariza ◽  
Elizabeth Suesca ◽  
Chad Leidy ◽  
John M. Gonzalez
Keyword(s):  
Cell Membrane ◽  
Cell Line ◽  
Targeted Delivery ◽  
Neuroblastoma Cell ◽  
Density Lipoprotein ◽  
Chemotherapeutic Agents ◽  
Neuroblastoma Cell Line ◽  
Endocytic Pathway ◽  
Astrocytoma Cell Line ◽  
Astrocytoma Cell

Liposomes are bilayer membrane vesicles that can serve as vehicles for drug delivery. They are a good alternative to free drug administration that provides cell-targeted delivery into tumors, limiting the systemic toxicity of chemotherapeutic agents. Previous results from our group showed that an astrocytoma cell line exhibits selective uptake of sulfatide-rich (SCB) liposomes, mediated by the low-density lipoprotein receptor (LDL-R). The goal of this study was to assess the uptake of liposomes in a neuroblastoma cell line. For this purpose, we used two types of liposomes, one representing a regular cell membrane (DOPC) and another rich in myelin components (SCB). An astrocytoma cell line was used as a control. Characterization of liposome uptake and distribution was conducted by flow cytometry and fluorescence microscopy. Similar levels of LDL-R expression were found in both cell lines. The uptake of SCB liposomes was higher than that of DOPC liposomes. No alterations in cell viability were found. SCB liposomes were located near the cell membrane and did not colocalize within the acidic cellular compartments. Two endocytic pathway inhibitors did not affect the liposome uptake. Neuroblastoma cells exhibited a similar uptake of SCB liposomes as astrocytoma cells; however, the pathway involved appeared to be different than the hypothesized pathway of LDL-R clathrin-mediated endocytosis.

scholarly journals N-acetylaspartate (NAA) induces neuronal differentiation of SH-SY5Y neuroblastoma cell line and sensitizes it to chemotherapeutic agents

Oncotarget ◽  
2016 ◽  
Vol 7 (18) ◽  
pp. 26235-26246 ◽  
Author(s):  
Carmela Mazzoccoli ◽  
Vitalba Ruggieri ◽  
Tiziana Tataranni ◽  
Francesca Agriesti ◽  
Ilaria Laurenzana ◽  
...  
Keyword(s):  
Cell Line ◽  
Neuronal Differentiation ◽  
Neuroblastoma Cell ◽  
Chemotherapeutic Agents ◽  
Neuroblastoma Cell Line

Effects of Prolactin on Ionic Membrane Conductances in the Human Malignant Astrocytoma Cell Line U87-MG

2004 ◽  
Vol 91 (3) ◽  
pp. 1203-1216 ◽  
Author(s):  
Thomas Ducret ◽  
Anne-Marie Vacher ◽  
Pierre Vacher
Keyword(s):  
Cell Membrane ◽  
Cell Line ◽  
Malignant Astrocytoma ◽  
Patch Clamp Technique ◽  
Peripheral Tissues ◽  
Membrane Hyperpolarization ◽  
Membrane Conductances ◽  
Astrocytoma Cell Line ◽  
Astrocytoma Cell ◽  
Ion Conductances

Prolactin (PRL) is involved in numerous biological processes in peripheral tissues and the brain. Although numerous studies have been conducted to elucidate the signal transduction pathways associated with the PRL receptor, very few have examined the role of ion conductances in PRL actions. We used the patch-clamp technique in “whole cell” configuration and microspectrofluorimetry to investigate the effects of PRL on membrane ion conductances in the U87-MG human malignant astrocytoma cell line, which naturally expresses the PRL receptor. We found that a physiological concentration (4 nM) of PRL exerted a biphasic action on membrane conductances. First, PRL activated a Ca2+-dependent K+ current that was sensitive to CTX and TEA. This current depended on PRL-induced Ca2+ mobilization, through a JAK2-dependent pathway from a thapsigargin- and 2-APB-sensitive Ca2+ pool. Second, PRL also activated an inwardly directed current, mainly due to the stimulation of calcium influx via nickel- and 2-APB-sensitive calcium channels. Both phases resulted in membrane hyperpolarizations, mainly through the activation of Ca2+-dependent K+ channels. As shown by combined experiments (electrophysiology and microspectrofluorimetry), the PRL-induced Ca2+ influx increased with cell membrane hyperpolarization and conversely decreased with cell membrane depolarization. Thus PRL-induced membrane hyperpolarizations facilitated Ca2+ influx through voltage-independent Ca2+ channels. Finally, PRL also activated a DIDS-sensitive Cl- current, which may participate in the PRL-induced hyperpolarization. These PRL-induced conductance activations are probably related to the PRL proliferative effect we have already described in U87-MG cells.

scholarly journals Pyrido[2′,1′:2,3]imidazo[4,5-c]isoquinolin-5-amines as Potential Cytotoxic Agents against Human Neuroblastoma

2021 ◽  
Vol 14 (8) ◽  
pp. 750
Author(s):  
Zahira Tber ◽  
Mohammed Loubidi ◽  
Jabrane Jouha ◽  
Ismail Hdoufane ◽  
Mümin Alper Erdogan ◽  
...  
Keyword(s):  
Cell Line ◽  
Drug Exposure ◽  
Caspase 3 ◽  
Biological Activities ◽  
Neuroblastoma Cell ◽  
Neuroblastoma Cell Line ◽  
Cytotoxic Agents ◽  
Human Neuroblastoma Cell ◽  
Human Neuroblastoma ◽  
Parp 1

We report herein the evaluation of various pyrido[2′,1′:2,3]imidazo[4,5-c]isoquinolin-5-amines as potential cytotoxic agents. These molecules were obtained by developing the multicomponent Groebke–Blackburn–Bienaymé reaction to yield various pyrido[2′,1′:2,3]imidazo[4,5-c]quinolines which are isosteres of ellipticine whose biological activities are well established. To evaluate the anticancer potential of these pyrido[2′,1′:2,3]imidazo[4,5-c]isoquinolin-5-amine derivatives in the human neuroblastoma cell line, the cytotoxicity was examined using the WST-1 assay after 72 h drug exposure. A clonogenic assay was used to assess the ability of treated cells to proliferate and form colonies. Protein expressions (Bax, bcl-2, cleaved caspase-3, cleaved PARP-1) were analyzed using Western blotting. The colony number decrease in cells was 50.54%, 37.88% and 27.12% following exposure to compounds 2d, 2g and 4b respectively at 10 μM. We also show that treating the neuroblastoma cell line with these compounds resulted in a significant alteration in caspase-3 and PARP-1 cleavage.

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