scholarly journals Propagation and Molecular Characterization of Fowl Adenovirus Serotype 8b Isolates in Chicken Embryo Liver Cells Adapted on Cytodex™ 1 Microcarrier Using Stirred Tank Bioreactor

Processes ◽  
2020 ◽  
Vol 8 (9) ◽  
pp. 1065
Author(s):  
Chidozie C. Ugwu ◽  
Mohd Hair-Bejo ◽  
Mat I. Nurulfiza ◽  
Abdul R. Omar ◽  
Aini Ideris
Keyword(s):  
Large Volume ◽  
Chicken Embryo ◽  
Scale Up ◽  
Virus Titer ◽  
Stirred Tank ◽  
Molecular Characteristics ◽  
Stirred Tank Bioreactor ◽  
Fowl Adenovirus ◽  
Volume Production ◽  
Embryo Liver

Large volume production of vaccine virus is essential for prevention and control of viral diseases. The objectives of this study were to propagate Fowl adenovirus (FAdV) isolate (UPM08136) in chicken embryo liver (CEL) cells adapted to Cytodex™ 1 microcarriers using stirred tank bioreactor (STB) and molecularly characterize the virus. CEL cells were prepared and seeded onto prepared Cytodex™ 1 microcarriers and incubated first in stationary phase for 3 h and in STB at 37 °C, 5% CO2, and 20 rpm for 24 h. The CEL cells were infected with FAdV isolate (UPM08136) passage 5 (UPM08136CELP5) or passage 20 (UPM08136CELP20) and monitored until cell detachment. Immunofluorescence, TCID50, sequencing, alignment of hexon and fiber genes, and phylogenetic analysis were carried out. CEL cells were adapted well to Cytodex™ 1 microcarriers and successfully propagated the FAdV isolates in STB with virus titer of 107.5 (UPM08136CELP5B1) and 106.5 (UPM08136CELP20B1) TCID50/mL. These isolates clustered with the reference FAdV serotype 8b in the same evolutionary clade. The molecular characteristics remained unchanged, except for a point substitution at position 4 of the hexon gene of UPM08136CELP20B1, suggesting that propagation of the FAdV isolate in STB is stable and suitable for large volume production and could be a breakthrough in the scale-up process.

scholarly journals Production of Newcastle Disease Virus by Vero Cells Grown on Cytodex 1 Microcarriers in a 2-Litre Stirred Tank Bioreactor

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Mohd Azmir Arifin ◽  
Maizirwan Mel ◽  
Mohamed Ismail Abdul Karim ◽  
Aini Ideris
Keyword(s):  
Cell Culture ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
High Speed ◽  
Virus Titer ◽  
Vero Cells ◽  
Stirred Tank ◽  
Stirred Tank Bioreactor ◽  
Maximum Cell

The aim of this study is to prepare a model for the production of Newcastle disease virus (NDV) lentogenic F strain using cell culture in bioreactor for live attenuated vaccine preparation. In this study, firstly we investigated the growth of Vero cells in several culture media. The maximum cell number was yielded by culture of Vero cells in Dulbecco's Modified Eagle Medium (DMEM) which was1.93×106 cells/ml. Secondly Vero cells were grown in two-litre stirred tank bioreactor by using several commercial microcarriers. We achieved the maximum cell concentration about7.95×105 cells/ml when using Cytodex 1. Later we produced Newcastle Disease virus in stirred tank bioreactor based on the design developed using Taguchi L4 method. Results reveal that higher multiplicity of infection (MOI) and size of cell inoculums can yield higher virus titer. Finally, virus samples were purified using high-speed centrifugation based on3∗∗(3-1) Fractional Factorial Design. Statistical analysis showed that the maximum virus titer can be achieved at virus sample concentration of 58.45% (v/v), centrifugation speed of 13729 rpm, and centrifugation time of 4 hours. As a conclusion, high yield of virus titer could be achieved through optimization of cell culture in bioreactor and separation by high-speed centrifugation.

scholarly journals Ginsenoside content in suspension cultures of Panax quinquefolium L. cultivated in shake flasksand stirred-tank bioreactor

2018 ◽  
Vol 72 (1) ◽  
pp. 15 ◽  
Author(s):  
Ewa Kochan ◽  
Sylwia Caban ◽  
Grażyna Szymańska ◽  
Piotr Szymczyk ◽  
Anna Lipert ◽  
...  
Keyword(s):  
Large Scale ◽  
Raw Materials ◽  
Scale Up ◽  
Fold Increase ◽  
Suspension Cultures ◽  
Stirred Tank ◽  
Small Scale ◽  
Panax Quinquefolium ◽  
Stirred Tank Bioreactor ◽  
Shake Flasks

<p>Plant suspension cultures are described as a source for the acquisition of medicinal secondary metabolites which in the future may become an alternative to traditional raw materials. This study demonstrates that the cell cultures of one of the ginseng species – Panax quinquefolium L. synthesize ginsenosides, which are triterpene saponins having a multidirectional pharmacological effects. Tested suspension cultures were run on a small scale in the shake flasksand in scale up of the process in a 10-liter stirred tank. In the shake flasks,the highest biomass yield (2.28 gl-1 for dry and 33.99 gl-1 for fresh weight) was reached on day 30 of culture, and the highest content of saponins (2.66 mg g -1 dw) was determined on day 28 of culture. In the bioreactor, nearly 2.67 and 3-fold increase of respectively dry and fresh biomass was recorded in relation to the inoculum. Large-scale cultures synthesized protopanaxatriol derivatives such as Rg1 and Re ginsenosides, however, no saponins belonging to the protopanaxadiol derivatives were reported.</p>

scholarly journals Molecular characterization of fowl adenovirus isolate of Malaysia attenuated in chicken embryo liver cells and its pathogenicity and immunogenicity in chickens

PLoS ONE ◽  
2019 ◽  
Vol 14 (12) ◽  
pp. e0225863
Author(s):  
Norfitriah Mohamed Sohaimi ◽  
Mohd Hair Bejo ◽  
Abdul Rahman Omar ◽  
Aini Ideris ◽  
Nurulfiza Mat Isa
Keyword(s):  
Molecular Characterization ◽  
Chicken Embryo ◽  
Liver Cells ◽  
Fowl Adenovirus ◽  
Embryo Liver

scholarly journals Pathogenicity and Immunogenicity of Attenuated Fowl Adenovirus from Chicken Embryo Liver Cells in Commercial Broiler Chickens

2021 ◽  
Vol 9 (5) ◽  
Author(s):  
Norfitriah Mohamed Sohaimi ◽  
Mohd Hair Bejo ◽  
Abdul Rahman Omar ◽  
Aini Ideris ◽  
Nurulfiza Mat Isa
Keyword(s):  
Chicken Embryo ◽  
Broiler Chickens ◽  
Liver Cells ◽  
Fowl Adenovirus ◽  
Commercial Broiler ◽  
Embryo Liver

Scale‐up processing with different microparticle agent for β‐mannanase production in a large‐scale stirred tank bioreactor

2020 ◽  
Author(s):  
Hilal Nur Gürler ◽  
Selime Benemir Erkan ◽  
Ali Ozcan ◽  
Cansu Yılmazer ◽  
Ercan Karahalil ◽  
...  
Keyword(s):  
Large Scale ◽  
Scale Up ◽  
Stirred Tank ◽  
Stirred Tank Bioreactor
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