Scale‐up processing with different microparticle agent for β‐mannanase production in a large‐scale stirred tank bioreactor

2020 ◽  
Author(s):  
Hilal Nur Gürler ◽  
Selime Benemir Erkan ◽  
Ali Ozcan ◽  
Cansu Yılmazer ◽  
Ercan Karahalil ◽  
...  
Keyword(s):  
Large Scale ◽  
Scale Up ◽  
Stirred Tank ◽  
Stirred Tank Bioreactor

scholarly journals Ginsenoside content in suspension cultures of Panax quinquefolium L. cultivated in shake flasksand stirred-tank bioreactor

2018 ◽  
Vol 72 (1) ◽  
pp. 15 ◽  
Author(s):  
Ewa Kochan ◽  
Sylwia Caban ◽  
Grażyna Szymańska ◽  
Piotr Szymczyk ◽  
Anna Lipert ◽  
...  
Keyword(s):  
Large Scale ◽  
Raw Materials ◽  
Scale Up ◽  
Fold Increase ◽  
Suspension Cultures ◽  
Stirred Tank ◽  
Small Scale ◽  
Panax Quinquefolium ◽  
Stirred Tank Bioreactor ◽  
Shake Flasks

<p>Plant suspension cultures are described as a source for the acquisition of medicinal secondary metabolites which in the future may become an alternative to traditional raw materials. This study demonstrates that the cell cultures of one of the ginseng species – Panax quinquefolium L. synthesize ginsenosides, which are triterpene saponins having a multidirectional pharmacological effects. Tested suspension cultures were run on a small scale in the shake flasksand in scale up of the process in a 10-liter stirred tank. In the shake flasks,the highest biomass yield (2.28 gl-1 for dry and 33.99 gl-1 for fresh weight) was reached on day 30 of culture, and the highest content of saponins (2.66 mg g -1 dw) was determined on day 28 of culture. In the bioreactor, nearly 2.67 and 3-fold increase of respectively dry and fresh biomass was recorded in relation to the inoculum. Large-scale cultures synthesized protopanaxatriol derivatives such as Rg1 and Re ginsenosides, however, no saponins belonging to the protopanaxadiol derivatives were reported.</p>

Large-scale propagation of a replication-defective adenovirus vector in stirred-tank bioreactor PER.C6? cell culture under sparging conditions

2003 ◽  
Vol 83 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Liangzhi Xie ◽  
Christian Metallo ◽  
James Warren ◽  
Warren Pilbrough ◽  
Joseph Peltier ◽  
...  
Keyword(s):  
Cell Culture ◽  
Large Scale ◽  
Adenovirus Vector ◽  
Stirred Tank ◽  
Stirred Tank Bioreactor ◽  
Replication Defective Adenovirus

scholarly journals Propagation and Molecular Characterization of Fowl Adenovirus Serotype 8b Isolates in Chicken Embryo Liver Cells Adapted on Cytodex™ 1 Microcarrier Using Stirred Tank Bioreactor

Processes ◽  
2020 ◽  
Vol 8 (9) ◽  
pp. 1065
Author(s):  
Chidozie C. Ugwu ◽  
Mohd Hair-Bejo ◽  
Mat I. Nurulfiza ◽  
Abdul R. Omar ◽  
Aini Ideris
Keyword(s):  
Large Volume ◽  
Chicken Embryo ◽  
Scale Up ◽  
Virus Titer ◽  
Stirred Tank ◽  
Molecular Characteristics ◽  
Stirred Tank Bioreactor ◽  
Fowl Adenovirus ◽  
Volume Production ◽  
Embryo Liver

Large volume production of vaccine virus is essential for prevention and control of viral diseases. The objectives of this study were to propagate Fowl adenovirus (FAdV) isolate (UPM08136) in chicken embryo liver (CEL) cells adapted to Cytodex™ 1 microcarriers using stirred tank bioreactor (STB) and molecularly characterize the virus. CEL cells were prepared and seeded onto prepared Cytodex™ 1 microcarriers and incubated first in stationary phase for 3 h and in STB at 37 °C, 5% CO2, and 20 rpm for 24 h. The CEL cells were infected with FAdV isolate (UPM08136) passage 5 (UPM08136CELP5) or passage 20 (UPM08136CELP20) and monitored until cell detachment. Immunofluorescence, TCID50, sequencing, alignment of hexon and fiber genes, and phylogenetic analysis were carried out. CEL cells were adapted well to Cytodex™ 1 microcarriers and successfully propagated the FAdV isolates in STB with virus titer of 107.5 (UPM08136CELP5B1) and 106.5 (UPM08136CELP20B1) TCID50/mL. These isolates clustered with the reference FAdV serotype 8b in the same evolutionary clade. The molecular characteristics remained unchanged, except for a point substitution at position 4 of the hexon gene of UPM08136CELP20B1, suggesting that propagation of the FAdV isolate in STB is stable and suitable for large volume production and could be a breakthrough in the scale-up process.

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