Process optimization and scale-up production of fungal aryl alcohol oxidase from genetically modified Aspergillus nidulans in stirred-tank bioreactor

2020 ◽  
Vol 315 ◽  
pp. 123792 ◽  
Author(s):  
Enshi Liu ◽  
Mark R. Wilkins
Keyword(s):  
Aspergillus Nidulans ◽  
Process Optimization ◽  
Genetically Modified ◽  
Scale Up ◽  
Alcohol Oxidase ◽  
Stirred Tank ◽  
Stirred Tank Bioreactor

scholarly journals Ginsenoside content in suspension cultures of Panax quinquefolium L. cultivated in shake flasksand stirred-tank bioreactor

2018 ◽  
Vol 72 (1) ◽  
pp. 15 ◽  
Author(s):  
Ewa Kochan ◽  
Sylwia Caban ◽  
Grażyna Szymańska ◽  
Piotr Szymczyk ◽  
Anna Lipert ◽  
...  
Keyword(s):  
Large Scale ◽  
Raw Materials ◽  
Scale Up ◽  
Fold Increase ◽  
Suspension Cultures ◽  
Stirred Tank ◽  
Small Scale ◽  
Panax Quinquefolium ◽  
Stirred Tank Bioreactor ◽  
Shake Flasks

<p>Plant suspension cultures are described as a source for the acquisition of medicinal secondary metabolites which in the future may become an alternative to traditional raw materials. This study demonstrates that the cell cultures of one of the ginseng species – Panax quinquefolium L. synthesize ginsenosides, which are triterpene saponins having a multidirectional pharmacological effects. Tested suspension cultures were run on a small scale in the shake flasksand in scale up of the process in a 10-liter stirred tank. In the shake flasks,the highest biomass yield (2.28 gl-1 for dry and 33.99 gl-1 for fresh weight) was reached on day 30 of culture, and the highest content of saponins (2.66 mg g -1 dw) was determined on day 28 of culture. In the bioreactor, nearly 2.67 and 3-fold increase of respectively dry and fresh biomass was recorded in relation to the inoculum. Large-scale cultures synthesized protopanaxatriol derivatives such as Rg1 and Re ginsenosides, however, no saponins belonging to the protopanaxadiol derivatives were reported.</p>

Process optimization in a stirred tank bioreactor based on CFD-Taguchi method: A case study

2019 ◽  
Vol 230 ◽  
pp. 1074-1084 ◽  
Author(s):  
Lei Shu ◽  
Mingjin Yang ◽  
Hang Zhao ◽  
Tianfu Li ◽  
Ling Yang ◽  
...  
Keyword(s):  
Taguchi Method ◽  
Process Optimization ◽  
Stirred Tank ◽  
Stirred Tank Bioreactor

Scale‐up processing with different microparticle agent for β‐mannanase production in a large‐scale stirred tank bioreactor

2020 ◽  
Author(s):  
Hilal Nur Gürler ◽  
Selime Benemir Erkan ◽  
Ali Ozcan ◽  
Cansu Yılmazer ◽  
Ercan Karahalil ◽  
...  
Keyword(s):  
Large Scale ◽  
Scale Up ◽  
Stirred Tank ◽  
Stirred Tank Bioreactor
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