scholarly journals Estimating Limits of Detection and Quantification of Ibuprofen by TLC-Densitometry at Different Chromatographic Conditions

Processes ◽  
2020 ◽  
Vol 8 (8) ◽  
pp. 919 ◽  
Author(s):  
Josef Jampilek ◽  
Malgorzata Dolowy ◽  
Alina Pyka-Pajak
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Mobile Phase ◽  
Modified Silica Gel ◽  
Limits Of Detection ◽  
Wide Range ◽  
The Impact ◽  
Layer Chromatography ◽  
Detection And Quantification

Ibuprofen is one of the best-known nonsteroidal anti-inflammatory and analgesic drugs. Following the previous work, the current study is focused on estimating the effect of different chromatographic conditions on the sensitivity of thin-layer chromatography in combination with UV densitometry, i.e., the detection and quantification of ibuprofen in a wide range of its concentrations including the lowest limits of detection (LOD) and quantification (LOQ). For this purpose, a reliable and easy-to-use calculation procedure for LOD and LOQ determination is presented in this work. In addition, the impact of type plates and mobile phase composition on the LOD and LOQ, respectively, of this active substance is accurately described. The results of detection and the quantification level of ibuprofen obtained under applied chromatographic conditions confirmed the utility of silica gel plates as well as silica gel bonded phases (i.e., reversed-phase (RP) plates) in the thin-layer chromatography (TLC)-densitometric analysis of ibuprofen at a low level, i.e., from several nanograms (below one microgram) to a few micrograms/spot. Among all chromatographic systems tested, the best are those consisting of silica gel 60F254 plates with concentrating zone (1.05583) and the mixture of n-hexane:ethyl acetate:glacial acetic acid in ratio 36:12:2 (v/v/v) as well as RP plates, i.e., RP-18F254 aluminum plates developed with methanol:water in volume composition 40:10 (v/v). These chromatographic systems allowed quantifying ibuprofen in the amount of 0.229 µg/spot and 0.228 µg/spot, which is less than 1 µg/spot. It can be stated that different chromatographic systems in combination with UV densitometric scanning at 224 nm proposed in this work can be successfully applied for the cost-effective and sensitive determination of ibuprofen as a widely used drug component as well as a residual in domestic wastewater. It was found that the modification of silica gel as well as the layer thickness of unmodified or modified silica gel 60 can influence the quality of chromatograms and the detection/quantification of ibuprofen in both normal phase (NP) and RP systems. Therefore, to obtain the best possible LOD and LOQ values of ibuprofen with precoated layers, suitable mobile phase and chromatographic plates are required.

Thin-layer chromatography of sulphonamides on silica gel G and polyamide layers by means of a polar mobile phase

1975 ◽  
Vol 106 (2) ◽  
pp. 349-355 ◽  
Author(s):  
G.L. Biagi ◽  
A.M. Barbaro ◽  
M.C. Guerra ◽  
G. Cantelli-Forti ◽  
O. Gandolfi
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Mobile Phase ◽  
Layer Chromatography

scholarly journals ANALYSIS OF DISODIUM 5’-GUANYLATE AND DISODIUM 5’-INOSINATE AS FLAVOR ENHANCER IN FOOD SPICES BY THIN-LAYER CHROMATOGRAPHY–DENSITOMETRY

2018 ◽  
Vol 10 (1) ◽  
pp. 51
Author(s):  
Viona Prima Dyta ◽  
Harmita . ◽  
Baitha Palanggatan M
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Analytical Method ◽  
Mobile Phase ◽  
Correlation Coefficients ◽  
Optimum Conditions ◽  
Coefficient Variation ◽  
Layer Chromatography ◽  
Flavor Enhancer

Objective: The objective of this study was to obtain an optimum and valid method of analysis to determine the levels of disodium 5’-guanylate (DSG)and disodium 5’-inosinate (DSI) in six samples of spices.Methods: The optimum method was obtained using silica gel 60 F254 as the stationary phase and isopropanol:water:25% ammonia at a ratio of 6:3:1(v/v) as the mobile phase. The developed spots were scanned using a densitometer in absorbance mode at 260 nm. The methods were valid basedon the accuracy criteria (DSG, 99.11–99.96%, and DSI, 98.56–101.05%), precision (DSG, 1.09%, and DSI, 0.49%), and linearity (DSG, r=0.9909, andDSI, r=0.9976).Results: The results showed that the levels of DSG in samples A, B, C, D, E, and F were 0.70%, 0.79%, 0.78%, 0.99%, 1.08%, and 1.08% and those ofDSI were 0.66%, 0.74%, 0.71%, 0.66%, 0.54%, and 0.67%, respectively.Conclusion: The optimum conditions of DSG and DSI for thin-layer chromatography-densitometry were obtained with silica gel 60 F254 as thestationary phase, isopropanol:water:25% ammonia (6:3:1) as the mobile phase, and a maximum wavelength of about 260 nm. Validation resultsindicated that the accuracy of the analytical method for DSG was about 99.11–99.96% with a coefficient variation (precision) of 0.70–1.41%, whilethat for DSI was 98.56–101.05% with a coefficient variation of 0.23–0.75%. The correlation coefficients for the analytical method for DSG and DSIwere 0.9909 and 0.9976, respectively. The results determined that the levels of DSG and DSI in samples A, B, C, D, E, and F were 0.70%/0.60%,0.79%/0.74%, 0.78%/0.71%, 0.99%/0.66%; 1.08%/0.54%, and 1.08%/0.67%, respectively.

Chemically Modified Silica Gel with Mercaptopropyltrimethoxysilane as Stationary Phase in Thin-Layer Chromatography

1999 ◽  
Vol 82 (2) ◽  
pp. 399-401 ◽  
Author(s):  
Constantin Marutoiu ◽  
Rodica Constantinescu ◽  
Florenta Dogar ◽  
Rodica Grecu
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Surface Characteristics ◽  
Modified Silica ◽  
Modified Silica Gel ◽  
Chemically Modified ◽  
Chemically Modified Silica ◽  
Chemically Modified Silica Gel ◽  
Layer Chromatography

Abstract A polar silica gel R chemically modified with mercaptopropyltrimethoxysilane was prepared. Surface characteristics of the silica gel R before and after chemical modification were determinated by elemental analysis, specific surface area characterization, infrared spectroscopy, and thin-layer chromatography. Eight dyes were separated on the chemically modified silica gel R.

scholarly journals Determination of Oxycodone Hydrochloride in Oral Solutions by High-Performance Thin-Layer Chromatography/Densitometry

2000 ◽  
Vol 83 (6) ◽  
pp. 1497-1501
Author(s):  
Hannele E M Salomies ◽  
Piia K Salo
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Mobile Phase ◽  
High Performance ◽  
Acetic Acid Water ◽  
Oxycodone Hydrochloride ◽  
Layer Chromatography

Abstract A thin-layer chromatography/densitometry method was developed and validated for the determination of oxycodone hydrochloride in oral solutions by using silica gel plates. A horizontal technique was used for development of the plates. The optimum composition for the mobile phase, which provided a suitable Rf value of 0.6 for oxycodone, was propanol–acetic acid–water–25% ammonia–methanol (20 + 1 + 1 + 3 + 10). Detection was at 234 nm. Oxycodone hydrochloride was stable on the sorbent and was precisely and accurately measured in the range of 0.3–1.5 μg/band.

Radial multi-stationary phase thin-layer chromatography for the field-ready fingerprinting of herbal materials

2019 ◽  
Vol 11 (43) ◽  
pp. 5511-5520 ◽  
Author(s):  
Sarah May Sibug-Torres ◽  
Isagani D. Padolina ◽  
Erwin P. Enriquez
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Stationary Phases ◽  
Modified Silica ◽  
Modified Silica Gel ◽  
Field Screening ◽  
Plate Design ◽  
Layer Chromatography

A novel TLC plate design that features multiple screen printed phenyl- and octyl-modified silica gel stationary phases configured as radial sectors was developed for the in-field screening of herbal materials.

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