Demixing effects in thin-layer chromatography with NH2-modified silica gel precoated plates

1986 ◽  
Vol 21 (6) ◽  
pp. 331-334 ◽  
Author(s):  
L. Zlatanov ◽  
C. Gonnet ◽  
M. Marichy
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Modified Silica ◽  
Modified Silica Gel ◽  
Layer Chromatography

Chemically Modified Silica Gel with Mercaptopropyltrimethoxysilane as Stationary Phase in Thin-Layer Chromatography

1999 ◽  
Vol 82 (2) ◽  
pp. 399-401 ◽  
Author(s):  
Constantin Marutoiu ◽  
Rodica Constantinescu ◽  
Florenta Dogar ◽  
Rodica Grecu
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Surface Characteristics ◽  
Modified Silica ◽  
Modified Silica Gel ◽  
Chemically Modified ◽  
Chemically Modified Silica ◽  
Chemically Modified Silica Gel ◽  
Layer Chromatography

Abstract A polar silica gel R chemically modified with mercaptopropyltrimethoxysilane was prepared. Surface characteristics of the silica gel R before and after chemical modification were determinated by elemental analysis, specific surface area characterization, infrared spectroscopy, and thin-layer chromatography. Eight dyes were separated on the chemically modified silica gel R.

Radial multi-stationary phase thin-layer chromatography for the field-ready fingerprinting of herbal materials

2019 ◽  
Vol 11 (43) ◽  
pp. 5511-5520 ◽  
Author(s):  
Sarah May Sibug-Torres ◽  
Isagani D. Padolina ◽  
Erwin P. Enriquez
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Stationary Phases ◽  
Modified Silica ◽  
Modified Silica Gel ◽  
Field Screening ◽  
Plate Design ◽  
Layer Chromatography

A novel TLC plate design that features multiple screen printed phenyl- and octyl-modified silica gel stationary phases configured as radial sectors was developed for the in-field screening of herbal materials.

scholarly journals Estimating Limits of Detection and Quantification of Ibuprofen by TLC-Densitometry at Different Chromatographic Conditions

Processes ◽  
2020 ◽  
Vol 8 (8) ◽  
pp. 919 ◽  
Author(s):  
Josef Jampilek ◽  
Malgorzata Dolowy ◽  
Alina Pyka-Pajak
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Mobile Phase ◽  
Modified Silica Gel ◽  
Limits Of Detection ◽  
Wide Range ◽  
The Impact ◽  
Layer Chromatography ◽  
Detection And Quantification

Ibuprofen is one of the best-known nonsteroidal anti-inflammatory and analgesic drugs. Following the previous work, the current study is focused on estimating the effect of different chromatographic conditions on the sensitivity of thin-layer chromatography in combination with UV densitometry, i.e., the detection and quantification of ibuprofen in a wide range of its concentrations including the lowest limits of detection (LOD) and quantification (LOQ). For this purpose, a reliable and easy-to-use calculation procedure for LOD and LOQ determination is presented in this work. In addition, the impact of type plates and mobile phase composition on the LOD and LOQ, respectively, of this active substance is accurately described. The results of detection and the quantification level of ibuprofen obtained under applied chromatographic conditions confirmed the utility of silica gel plates as well as silica gel bonded phases (i.e., reversed-phase (RP) plates) in the thin-layer chromatography (TLC)-densitometric analysis of ibuprofen at a low level, i.e., from several nanograms (below one microgram) to a few micrograms/spot. Among all chromatographic systems tested, the best are those consisting of silica gel 60F254 plates with concentrating zone (1.05583) and the mixture of n-hexane:ethyl acetate:glacial acetic acid in ratio 36:12:2 (v/v/v) as well as RP plates, i.e., RP-18F254 aluminum plates developed with methanol:water in volume composition 40:10 (v/v). These chromatographic systems allowed quantifying ibuprofen in the amount of 0.229 µg/spot and 0.228 µg/spot, which is less than 1 µg/spot. It can be stated that different chromatographic systems in combination with UV densitometric scanning at 224 nm proposed in this work can be successfully applied for the cost-effective and sensitive determination of ibuprofen as a widely used drug component as well as a residual in domestic wastewater. It was found that the modification of silica gel as well as the layer thickness of unmodified or modified silica gel 60 can influence the quality of chromatograms and the detection/quantification of ibuprofen in both normal phase (NP) and RP systems. Therefore, to obtain the best possible LOD and LOQ values of ibuprofen with precoated layers, suitable mobile phase and chromatographic plates are required.

DEVELOPMENT OF HPTLC METHOD FOR THE ESTIMATION OF ONDANSETRON AND RANITIDINE IN COMBINED DOSAGE FORM

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (12) ◽  
pp. 42-48
Author(s):  
P. J. Patel ◽  
◽  
D. A Shah ◽  
F. A. Mehta ◽  
U. K. Chhalotiya
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
High Performance ◽  
Dosage Form ◽  
Solvent System ◽  
Rf Values ◽  
Layer Chromatography ◽  
Hptlc Method

A simple, sensitive and precise high performance thin layer chromatographic (HPTLC)method has been developed for the estimation of ondansetron (OND) and ranitidine (RAN) in combination. The method was employed on thin layer chromatography (TLC) and aluminium plates were precoated with silica gel 60 F254 as the stationary phase, while the solvent system was methanol. The Rf values were observed to be 0.5 ± 0.02, and 0.3 ± 0.02 for OND and RAN, respectively. The separated spots were densitometrically analyzed in absorbance mode at 299 nm. This method was linear in the range of 25-300 ng/band for OND and 50-600 ng/band for RAN. The limits of detection for OND and RAN were found to be 3.47 and 1.83 ng/band, respectively. The limits of quantification for OND and RAN were found to be 10.53 and 5.55 ng/band, respectively. The proposed method was validated with respect to linearity, accuracy, precision and robustness. The method was successfully applied to the estimation of OND and RAN in combined dosage form.

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