Effect of inorganic salts as mobile-phase additives on lipophilicity values determined by reversed-phase thin-layer chromatography for new 1,2,4-triazole derivatives

2006 ◽  
Vol 19 (111) ◽  
pp. 386-392 ◽  
Author(s):  
Jolanta Flieger ◽  
Małgorzata Tatarczak
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Reversed Phase ◽  
Inorganic Salts ◽  
Triazole Derivatives ◽  
Mobile Phase Additives ◽  
Layer Chromatography

Thin-layer chromatography of aflatoxins and zearalenones with β-cyclodextrins as mobile phase additives

2011 ◽  
Vol 4 (2) ◽  
pp. 113-117 ◽  
Author(s):  
D. Larionova ◽  
I. Goryacheva ◽  
C. Van Peteghem ◽  
S. De Saeger
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Reversed Phase ◽  
Limit Of Quantification ◽  
Mobile Phase Additives ◽  
Phase Plates ◽  
Aflatoxin B ◽  
Layer Chromatography ◽  
Quantitative Fluorescence

The conditions of thin-layer chromatography separation of related aflatoxins and zearalenones in the presence of β-cyclodextrin and 2-hydroxypropyl-β-cyclodextrin were studied. Effects of the stationary phase and mobile phase composition were investigated. Analytical conditions for the separation and simultaneous semi-quantitative fluorescence detection of aflatoxins B1, B2, G1 and G2, zearalenone and α-zearalenol on normal-phase plates (silica gel, polyamide) and reversed-phase plates (C18) with cyclodextrin modified mobile phase were optimised. The limit of quantification was found 2 ng per spot for aflatoxin G1 and aflatoxin B2, 3.5 ng for aflatoxin B1 and aflatoxin G2 and 100 ng per spot for zearalenone and α-zearalenol. Addition of cyclodextrins to the mobile phase allowed a decrease in the amount of toxic solvents, and improved separation characteristics, but did not improve the limit of quantification.

scholarly journals Separation of 9-Fluorenylmethyloxycarbonyl Amino Acid Derivatives in Micellar Systems of High-Performance Thin-Layer Chromatography and Pressurized Planar Electrochromatography

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Beata Polak ◽  
Adam Traczuk ◽  
Sylwia Misztal
Keyword(s):  
Amino Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Amino Acid Derivatives ◽  
Solute Retention ◽  
Micellar Systems ◽  
Layer Chromatography

AbstractThe problems with separation of amino acid mixtures in reversed-phase mode are the result of their hydrophilic nature. The derivatisation of the amino group of mentioned above solutes leads to their solution. For this purpose, 9-fluorenylmethoxycarbonyl chloroformate (f-moc-Cl) as the derivatisation reagent is often used. In our study, the separation of some f-moc- amino acid derivatives (alanine, phenylalanine, leucine, methionine, proline and tryptophan) with the use of micellar systems of reversed-phase high-performance thin-layer chromatography (HPTLC) and pressurized planar electrochromatography (PPEC) is investigated. The effect of surfactant concentration, its type (anionic, cationic and non-ionic) and mobile phase buffer pH on the discussed above solute migration distances are presented. Our work reveals that the increase of sodium dodecylsulphate concentration in the mobile phase has a different effect on solute retention in HPTLC and PPEC. Moreover, it also affects the order of solutes in both techniques. In PPEC, in contrast to the HPTLC technique, the mobile phase pH affects solute retention. The type of surfactant in the mobile phase also impacts solute retention and migration distances. A mobile phase containing SDS improves system efficiency in both techniques. Herein, such an effect is presented for the first time.

scholarly journals Separation of some vitamins in reversed-phase thin-layer chromatography and pressurized planar electrochromatography with eluent containing surfactant

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Beata Polak ◽  
Emilia Pajurek
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Theoretical Plate ◽  
Reversed Phase ◽  
Water Soluble ◽  
System Capacity ◽  
Micellar Systems ◽  
Layer Chromatography

AbstractThe separation of some water- and fat-soluble vitamins via micellar systems of reversed-phase high-performance thin-layer chromatography (HPTLC) and pressurized planar electrochromatography (PPEC) was subjected to research. Hence, the influence of the mobile phase composition (surfactant and acetonitrile concentration, eluent buffer pH) on the migration distances and zone separation of some vitamins (thiamine, riboflavin, niacin, pyridoxine, cyanocobalamin, folic acid, ergocalciferol and α-tocopherol) was investigated. Our results indicated that the applied technique has an impact on the solute order. Comparing the system capacity of HPLC and PPEC (measured as height of the theoretical plate) for the mobile phase systems with and without surfactant shows differences, especially for fat-soluble vitamin. The variances and reproducibilities (% RDS) values of the vitamin are less in PPEC than in TLC. Moreover, the migration distances of water-soluble vitamins are longer than fat-soluble ones. Overall, eluent consisting of 50% acetonitrile, 18.75 mM SDS, the buffer of pH 6.99 via the PPEC technique was most appropriate for determining the investigated vitamins in the artificial mixture and the two commercially available vitamin combinations.

scholarly journals Assay of Tinidazole in Human Serum by High-Performance Thin-Layer Chromatography—Comparison with High-Performance Liquid Chromatography

1999 ◽  
Vol 82 (2) ◽  
pp. 244-247 ◽  
Author(s):  
M H Guermouche ◽  
D Habel ◽  
S Guermouche
Keyword(s):  
High Performance Liquid Chromatography ◽  
Thin Layer ◽  
Human Serum ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Layer Chromatography

Abstract Determination of tinidazole in human serum by high-performance thin-layer chromatography (HPTLC) is presented. It includes use of 10 × 10 cm plates coated with silica gel 60 and chloroform-acetonitrile-acetic acid (60 + 40 + 2) as mobile phase. Quantitation was performed by densitometry at 320 nm. The linearity (1-10 ng), precision (6%), reproducibility (5%), recovery (96%), and detection limit (1 mg/L) of tinidazole determination by HPTLC were comparable with corresponding method parameters by reversed-phase HPLC. A satisfactory correlation was found between the 2 analytical methods. The procedure was used to quantitate tinidazole in patient sera.

scholarly journals Determination of the Lipophilicity of Ibuprofen, Naproxen, Ketoprofen, and Flurbiprofen with Thin-Layer Chromatography

2019 ◽  
Vol 2019 ◽  
pp. 1-6 ◽  
Author(s):  
Andrzej Czyrski
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Human Body ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Regression Curve ◽  
Layer Chromatography

The lipophilicity is an important parameter that influences the activity of the drugs in the human body. The reversed phase high performance thin layer chromatography was applied to determine the Log P values of ibuprofen, ketoprofen, naproxen, and flurbiprofen. The stationary phase used in the study was silica-gel coated plates. The mobile phase was the mixture of acetonitrile and water in different proportions. The content of acetonitrile varied in 5% increments from 50% to 80%. The Rm0 values were determined for the compounds with a known Log P and for the analyzed substances (ibuprofen, naproxen, ketoprofen, and flurbiprofen). The Log P values were calculated for the analyzed compounds using the regression curve Rm0 = f(Log P) parameters for the compounds with the known lipophilicity. Flurbiprofen is characterized by the highest Log P value: 3.82. The lowest one is noted for ketoprofen: 2.66. The determined Log P values of tested compounds were similar to the values calculated by the software.

scholarly journals CENTRAL COMPOSITE DESIGN TO DEVELOP A ROBUST REVERSED-PHASE THIN-LAYER CHROMATOGRAPHY/DENSITOMETRY METHOD FOR QUANTIFICATION OF PARABENS IN COSMETIC PREPARATIONS

2017 ◽  
Vol 10 (10) ◽  
pp. 230 ◽  
Author(s):  
Kavitha Jayaseelan ◽  
Karunanidhi Santhana Lakshmi
Keyword(s):  
Thin Layer ◽  
Central Composite Design ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Reversed Phase ◽  
Local Market ◽  
Cosmetic Products ◽  
Validation Parameters ◽  
Layer Chromatography ◽  
Central Composite

  Objective: The current study was framed with a view to develop a simple, sensitive and a rapid reversed-phase thin-layer chromatography (RP-TLC)/ densitometry method for quantification of parabens (methyl, ethyl, propyl, and butyl) in various cosmetic preparations available in local market. Moreover, robustness testing was planned to be performed applying a central composite design (CCD).Methods: The desired chromatographic separation was achieved on RP-TLC/densitometry plates precoated with Silica gel 60 GF254 on aluminum sheet, employing methanol:toluene:acetonitrile:glacial acetic acid as mobile phase in the ratio of 3:5:1.8:0.2 v/v/v/v and detection was carried out at 256 nm.Results: The mobile phase used gave an excellent resolution of all the four separated parabens. The method was found linear over a wide range of 6.25- 400 ng/spot. The optimized method was validated by measuring various validation parameters. CCD was employed to check the robustness of the method at three-factor levels. The developed and optimized method was used to quantify parabens in 30 different cosmetic products procured from local market.Conclusion: The proposed and developed RP-TLC/densitometry method can be applied for routine analysis of all the parabens in cosmetic products and can also be extended to the analysis in pharmaceuticals and food products.

Reversed-phase stepwise gradient thin-layer chromatography of 19 test dye mixtures with a single void volume of the mobile phase

2017 ◽  
Vol 30 (2) ◽  
pp. 113-120 ◽  
Author(s):  
Aneta Hałka-Grysińska ◽  
Radosław Ł. Gwarda ◽  
Krzysztof Pawełek ◽  
Tomasz Baj ◽  
Tadeusz H. Dzido
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
Reversed Phase ◽  
Void Volume ◽  
Stepwise Gradient ◽  
Layer Chromatography
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