scholarly journals Simple Preparation of Diverse Neoagaro-Oligosaccharides

Processes ◽  
2019 ◽  
Vol 7 (5) ◽  
pp. 267 ◽  
Author(s):  
Fudi Lin ◽  
Jing Ye ◽  
Yayan Huang ◽  
Yucheng Yang ◽  
Meitian Xiao
Keyword(s):  
Activated Carbon ◽  
Column Chromatography ◽  
Substrate Concentration ◽  
Enzyme Hydrolysis ◽  
Simple Method ◽  
Nmr Structures ◽  
Simple Preparation ◽  
Authentic Standard

A simple method for obtaining pure and well-defined oligosaccharides was established by hydrolyzing agar with β-agarase from Vibrio natriegens. The conditions for enzymolysis were optimized as follows: a temperature of 45 °C, a pH of 8.5, a substrate concentration of 0.3%, an enzyme amount of 100 U/g and an enzymolysis time of 20 h. Neoagaro-oligosaccharides with different degrees of polymerization were obtained by hydrolyzing agar with β-agarase for different lengths of time. After removing pigments using activated carbon and salts by dialyzing, the enzyme hydrolysis solution was separated with Bio-Gel P2 column chromatography. Neoagaro-oligosaccharides with different degrees of polymerization were acquired. By comparing with authentic standard substances, along with further confirmation by FTIR, MS and NMR, structures of the purified neoagaro-oligosaccharides were identified as neoagarobiose (NA2), neoagaroteraose (NA4), neoagarohexaose (NA6), neoagarooctaose (NA8), neoagaro-decaose (NA10) and neoagarododecaose (NA12) with purities of more than 97.0%. The present study established a method for the preparation of various neoagaro-oligosaccharides that may be of great significance for further study of their bioactivities.

scholarly journals Simple and rapid separation of diverse neoagaro-oligosaccharides

2018 ◽  
Author(s):  
Fudi Lin ◽  
Yayan Huang ◽  
Na Zhang ◽  
Jing Ye ◽  
Meitian Xiao
Keyword(s):  
Activated Carbon ◽  
Column Chromatography ◽  
Substrate Concentration ◽  
Enzyme Hydrolysis ◽  
Rapid Separation ◽  
Rapid Preparation ◽  
Simple Method ◽  
Nmr Structures

AbstractA rapid and simple method for obtaining pure and well-defined oligosaccharides was established by hydrolyzing agar with β-agarase fromVibrio natriegens. The conditions for enzymolysis were optimized as follows: temperature of 45 °C, pH of 8.5, substrate concentration of 0.3%, enzyme amount of 100 U/g and enzymolysis time of 20 h. Neoagaro-oligosaccharides with different degree of polymerizations were gained by hydrolyzing agar with β-agarase at different enzymolysis time. After removing pigment by activated carbon and salts by dialyzing, the enzyme hydrolysis solution was separated with Bio-Gel P2 column chromatography. Neoagaro-oligosaccharides with different degree of polymerizations were acquired. By comparing with standard substances, along with further confirmation by FTIR, MS and NMR, structures of the purified neoagaro-oligosaccharides were identified as neoagarobiose, neoagaroteraose, neoagarohexaose, neoagarooctaose, neoagarodecaose and neoagarododecaose with purities more than 97.0%, respectively. The present study established a method for rapid preparation of various monomers of neoagaro-oligosaccharides that may be of great significance for further study of bioactivities.

Determination of Benzo (a) pyrene in Foods

1978 ◽  
Vol 61 (1) ◽  
pp. 129-135
Author(s):  
Yukio Saito ◽  
Hiroshi Sekita ◽  
Mitsuharu Takeda ◽  
Mitsuru Uchiyama
Keyword(s):  
Sulfuric Acid ◽  
Silica Gel ◽  
Column Chromatography ◽  
Analytical Method ◽  
Selective Extraction ◽  
Acid Extraction ◽  
Simple Method ◽  
Extraction Step ◽  
Concentrated Sulfuric Acid

Abstract An analytical method was developed for determining benzo (a) pyrene in foods, suitable for routine use. The method consists of 4 cleanup steps: (1) alkali cleavage of sample, (2) preliminary silica gel column chromatography, (3) selective extraction with concentrated sulfuric acid, and (4) further silica gel column chromatography. Recoveries of benzo- (a) pyrene added to 50 g (or 10 g) food at levels of 0.4 ppb (or 2 ppb) ranged from 70% for short-necked clam and mackerel to 85% for chicken meat. The sulfuric acid extraction step affords a simple method for isolating benzo (a)- pyrene from various kinds of interfering substances which could not be separated by existing methods.

scholarly journals Rapid and simple preparation of thiol–ene emulsion-templated monoliths and their application as enzymatic microreactors

Lab on a Chip ◽  
2015 ◽  
Vol 15 (10) ◽  
pp. 2162-2172 ◽  
Author(s):  
Josiane P. Lafleur ◽  
Silja Senkbeil ◽  
Jakub Novotny ◽  
Gwenaël Nys ◽  
Nanna Bøgelund ◽  
...  
Keyword(s):  
Microfluidic Channels ◽  
Simple Method ◽  
Simple Preparation ◽  
Enzymatic Microreactors

A novel, rapid and simple method for the preparation of emulsion-templated monoliths in microfluidic channels based on thiol–ene chemistry is presented.

Purification of the thermostable direct hemolysin of Vibrio parahaemolyticus by immunoaffinity column chromatography

1986 ◽  
Vol 32 (1) ◽  
pp. 71-73 ◽  
Author(s):  
Takeshi Honda ◽  
Myonsun Yoh ◽  
Ikuyo Narita ◽  
Toshio Miwatani ◽  
Huilan Sima ◽  
...  
Keyword(s):  
Column Chromatography ◽  
Vibrio Parahaemolyticus ◽  
Culture Supernatant ◽  
Immunoaffinity Column ◽  
Simple Method ◽  
Thermostable Direct Hemolysin

A simple method involving immunoaffinity column chromatography to purify the thermostable direct hemolysin of Vibrio parahaemolyticus was developed. The thermostable direct hemolysin purified from the culture supernatant of a strain isolated from the first reported case of V. parahaemolyticus infection in China in 1985 was indistinguishable from the hemolysins purified from strains isolated in Japan.

A simple method for purifying silica gel for column chromatography

1988 ◽  
Vol 65 (10) ◽  
pp. 891 ◽  
Author(s):  
Antonio E. Guarconi ◽  
Victor F. Ferreira
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
Simple Method

A simple method for developing mesoporosity in activated carbon

2003 ◽  
Vol 31 (1) ◽  
pp. 47-52 ◽  
Author(s):  
Zhonghua Hu ◽  
Huimin Guo ◽  
M.P. Srinivasan ◽  
Ni Yaming
Keyword(s):  
Activated Carbon ◽  
Simple Method

ROUTINE DETERMINATIONS OF 17-KETOSTEROIDS AND PORTER-SILBER CHROMOGENS COMPARED WITH THE CHROMATOGRAPHIC MEASUREMENT OF GROUPED AND INDIVIDUAL STEROIDS

1964 ◽  
Vol 46 (4) ◽  
pp. 552-562 ◽  
Author(s):  
Ingrid Ernest ◽  
Britt Håkansson ◽  
Jörgen Lehmann ◽  
Björn Sjögren
Keyword(s):  
Urinary Excretion ◽  
Quantitative Determination ◽  
Column Chromatography ◽  
Chromatographic Separation ◽  
Enzyme Hydrolysis ◽  
Routine Method ◽  
Urinary Steroids ◽  
Urinary Content ◽  
Chromatographic Measurement

ABSTRACT The accuracy of two routine methods for the determination of urinary steroids – 17-ketosteroids (17-KS) and Porter-Silber chromogens – has been investigated by chromatographic separation and quantitative determination of individual 17-KS and Porter-Silber reacting steroids in 141 urine samples. For this purpose urine was submitted to enzyme hydrolysis and subsequent solvolysis. The pertinent steroids were separated by column chromatography into three groups, 11-deoxy-17-KS, 11-oxy-17-KS and Porter-Silber reacting steroids. The final separation was accomplished by paper chromatography. As a mean, the urinary excretion of true 17-KS corresponded to about 40–50 per cent of the routine figures. Due to non specific chromogens, individual routine figures were completely unreliable and probably had no more significance than showing a difference between a high and a low urinary content of 17-KS A true figure, however, was never higher than that indicated by the routine method. The routine method for determination of Porter-Silber chromogens also overestimated the urinary content of steroids, the true excretion of Porter-Silber steroids being, on an average, about 25 % lower. Again, the significance of individual determinations was low. The determination of 17-KS and Porter-Silber steroids by column chromatography was found to be rather simple and reliable as only minor amounts of unspecific chromogens were included in the results. Moreover with this method, the 17-KS were separated into 11-deoxy-17-KS and 11-oxy-17-KS.

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