scholarly journals Simple and rapid separation of diverse neoagaro-oligosaccharides

2018 ◽  
Author(s):  
Fudi Lin ◽  
Yayan Huang ◽  
Na Zhang ◽  
Jing Ye ◽  
Meitian Xiao
Keyword(s):  
Activated Carbon ◽  
Column Chromatography ◽  
Substrate Concentration ◽  
Enzyme Hydrolysis ◽  
Rapid Separation ◽  
Rapid Preparation ◽  
Simple Method ◽  
Nmr Structures

AbstractA rapid and simple method for obtaining pure and well-defined oligosaccharides was established by hydrolyzing agar with β-agarase fromVibrio natriegens. The conditions for enzymolysis were optimized as follows: temperature of 45 °C, pH of 8.5, substrate concentration of 0.3%, enzyme amount of 100 U/g and enzymolysis time of 20 h. Neoagaro-oligosaccharides with different degree of polymerizations were gained by hydrolyzing agar with β-agarase at different enzymolysis time. After removing pigment by activated carbon and salts by dialyzing, the enzyme hydrolysis solution was separated with Bio-Gel P2 column chromatography. Neoagaro-oligosaccharides with different degree of polymerizations were acquired. By comparing with standard substances, along with further confirmation by FTIR, MS and NMR, structures of the purified neoagaro-oligosaccharides were identified as neoagarobiose, neoagaroteraose, neoagarohexaose, neoagarooctaose, neoagarodecaose and neoagarododecaose with purities more than 97.0%, respectively. The present study established a method for rapid preparation of various monomers of neoagaro-oligosaccharides that may be of great significance for further study of bioactivities.

scholarly journals Simple Preparation of Diverse Neoagaro-Oligosaccharides

Processes ◽  
2019 ◽  
Vol 7 (5) ◽  
pp. 267 ◽  
Author(s):  
Fudi Lin ◽  
Jing Ye ◽  
Yayan Huang ◽  
Yucheng Yang ◽  
Meitian Xiao
Keyword(s):  
Activated Carbon ◽  
Column Chromatography ◽  
Substrate Concentration ◽  
Enzyme Hydrolysis ◽  
Simple Method ◽  
Nmr Structures ◽  
Simple Preparation ◽  
Authentic Standard

A simple method for obtaining pure and well-defined oligosaccharides was established by hydrolyzing agar with β-agarase from Vibrio natriegens. The conditions for enzymolysis were optimized as follows: a temperature of 45 °C, a pH of 8.5, a substrate concentration of 0.3%, an enzyme amount of 100 U/g and an enzymolysis time of 20 h. Neoagaro-oligosaccharides with different degrees of polymerization were obtained by hydrolyzing agar with β-agarase for different lengths of time. After removing pigments using activated carbon and salts by dialyzing, the enzyme hydrolysis solution was separated with Bio-Gel P2 column chromatography. Neoagaro-oligosaccharides with different degrees of polymerization were acquired. By comparing with authentic standard substances, along with further confirmation by FTIR, MS and NMR, structures of the purified neoagaro-oligosaccharides were identified as neoagarobiose (NA2), neoagaroteraose (NA4), neoagarohexaose (NA6), neoagarooctaose (NA8), neoagaro-decaose (NA10) and neoagarododecaose (NA12) with purities of more than 97.0%. The present study established a method for the preparation of various neoagaro-oligosaccharides that may be of great significance for further study of their bioactivities.

scholarly journals Rapid Separation of LDL Subclasses by Iodixanol Gradient Ultracentrifugation

2003 ◽  
Vol 49 (11) ◽  
pp. 1865-1872 ◽  
Author(s):  
Ian G Davies ◽  
John M Graham ◽  
Bruce A Griffin
Keyword(s):  
Density Gradient ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Digital Photography ◽  
Rapid Separation ◽  
Simple Method ◽  
Peak Density ◽  
Plasma Triglycerides ◽  
Threefold Increase ◽  
Chd Risk

Abstract Background: A predominance of small, dense LDL (sdLDL) confers in excess of a threefold increase in coronary heart disease (CHD) risk. The conventional method for the detection of sdLDL, salt density gradient ultracentrifugation (DGUC) has been superseded by more rapid techniques. This report presents novel methodology for the separation of sdLDL by a combination of iodixanol density gradient centrifugation and digital photography. Methods: LDL subclasses were separated in 3 h from prestained plasma on a self-forming density gradient of iodixanol. LDL subclass profiles were generated by digital photography and gel-scan software. Plasma samples from 106 normo- and dyslipidemic individuals were used to optimize the gradient for the resolution of LDL heterogeneity. A subgroup of 47 LDL profiles were then compared with LDL subclasses separated by salt DGUC. Results: The peak density of the predominant LDL band correlated significantly with the relative abundance (as a percentage) of sdLDL as resolved by salt DGUC (P <0.001). As shown previously, LDL isolated at a lighter density in iodixanol compared with salt gradients. A predominance of sdLDL corresponded to a peak density on iodixanol of 1.028 kg/L. This density and the area under the LDL profile lying above this density were sensitive and specific markers for the prediction of a predominance of sdLDL (P <0.001) and showed predictable associations with plasma triglycerides (r = 0.59; P <0.001) and HDL (r = −0.4; P <0.001). Conclusions: This simple method for the detection of sdLDL can differentiate a predominance of sdLDL, is highly reproducible, and can be used preparatively to isolate sdLDL.

Determination of Benzo (a) pyrene in Foods

1978 ◽  
Vol 61 (1) ◽  
pp. 129-135
Author(s):  
Yukio Saito ◽  
Hiroshi Sekita ◽  
Mitsuharu Takeda ◽  
Mitsuru Uchiyama
Keyword(s):  
Sulfuric Acid ◽  
Silica Gel ◽  
Column Chromatography ◽  
Analytical Method ◽  
Selective Extraction ◽  
Acid Extraction ◽  
Simple Method ◽  
Extraction Step ◽  
Concentrated Sulfuric Acid

Abstract An analytical method was developed for determining benzo (a) pyrene in foods, suitable for routine use. The method consists of 4 cleanup steps: (1) alkali cleavage of sample, (2) preliminary silica gel column chromatography, (3) selective extraction with concentrated sulfuric acid, and (4) further silica gel column chromatography. Recoveries of benzo- (a) pyrene added to 50 g (or 10 g) food at levels of 0.4 ppb (or 2 ppb) ranged from 70% for short-necked clam and mackerel to 85% for chicken meat. The sulfuric acid extraction step affords a simple method for isolating benzo (a)- pyrene from various kinds of interfering substances which could not be separated by existing methods.

Purification of the thermostable direct hemolysin of Vibrio parahaemolyticus by immunoaffinity column chromatography

1986 ◽  
Vol 32 (1) ◽  
pp. 71-73 ◽  
Author(s):  
Takeshi Honda ◽  
Myonsun Yoh ◽  
Ikuyo Narita ◽  
Toshio Miwatani ◽  
Huilan Sima ◽  
...  
Keyword(s):  
Column Chromatography ◽  
Vibrio Parahaemolyticus ◽  
Culture Supernatant ◽  
Immunoaffinity Column ◽  
Simple Method ◽  
Thermostable Direct Hemolysin

A simple method involving immunoaffinity column chromatography to purify the thermostable direct hemolysin of Vibrio parahaemolyticus was developed. The thermostable direct hemolysin purified from the culture supernatant of a strain isolated from the first reported case of V. parahaemolyticus infection in China in 1985 was indistinguishable from the hemolysins purified from strains isolated in Japan.

scholarly journals A filtration method for the separation of cell sap from the large-particle fraction of rat liver suspensions, enabling the rapid measurement of nucleotide distributions

1970 ◽  
Vol 116 (2) ◽  
pp. 299-302 ◽  
Author(s):  
V. B. Delaney ◽  
T. F. Slater
Keyword(s):  
Endoplasmic Reticulum ◽  
Rat Liver ◽  
Large Particle ◽  
Particle Fraction ◽  
Rapid Separation ◽  
Simple Method ◽  
Filtration Method ◽  
Rapid Measurement ◽  
A Cell ◽  
Millipore Filters

A simple method is described that allows a rapid separation of a cell-sap fraction from the large-particle fraction of rat liver suspensions. The method is based on the filtration under suction of liver suspensions through Millipore filters that retain nuclei, mitochondria and some of the endoplasmic-reticulum fraction, but allow quantitative passage of cell sap into a collecting tube. The cell sap may be separated in this manner within 2min of the death of the rat. The method was applied to study the intracellular distribution of ATP and of the nicotinamide–adenine dinucleotides and the results obtained were compared with those obtained after separating the cell sap by a rapid centrifuging procedure. The percentage of total liver ATP in the cell sap was found to be 46% by the filtration method and more than 70% by the centrifuging procedure. Corresponding figures found for the distribution of NADP++NADPH were 40 and 49% respectively.

A simple method for purifying silica gel for column chromatography

1988 ◽  
Vol 65 (10) ◽  
pp. 891 ◽  
Author(s):  
Antonio E. Guarconi ◽  
Victor F. Ferreira
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
Simple Method

A simple method for developing mesoporosity in activated carbon

2003 ◽  
Vol 31 (1) ◽  
pp. 47-52 ◽  
Author(s):  
Zhonghua Hu ◽  
Huimin Guo ◽  
M.P. Srinivasan ◽  
Ni Yaming
Keyword(s):  
Activated Carbon ◽  
Simple Method
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