scholarly journals Rapid and simple preparation of thiol–ene emulsion-templated monoliths and their application as enzymatic microreactors

Lab on a Chip ◽  
2015 ◽  
Vol 15 (10) ◽  
pp. 2162-2172 ◽  
Author(s):  
Josiane P. Lafleur ◽  
Silja Senkbeil ◽  
Jakub Novotny ◽  
Gwenaël Nys ◽  
Nanna Bøgelund ◽  
...  
Keyword(s):  
Microfluidic Channels ◽  
Simple Method ◽  
Simple Preparation ◽  
Enzymatic Microreactors

A novel, rapid and simple method for the preparation of emulsion-templated monoliths in microfluidic channels based on thiol–ene chemistry is presented.

Microfluidic channel-integrated hanging drop array chip operated by pushbuttons for spheroid culture and analysis

The Analyst ◽  
2020 ◽  
Vol 145 (21) ◽  
pp. 6974-6980
Author(s):  
Juhwan Park ◽  
Hwisoo Kim ◽  
Je-Kyun Park
Keyword(s):  
Microfluidic Channel ◽  
Microfluidic Channels ◽  
Hanging Drop ◽  
Simple Method ◽  
Spheroid Culture ◽  
Liquid Handling

We developed a simple method for liquid handling in a hanging drop array chip for spheroid culture and analysis by integrating microfluidic channels operated by pushbuttons.

A simple method to determine the surface charge in microfluidic channels

2010 ◽  
Vol 31 (3) ◽  
pp. 563-569 ◽  
Author(s):  
Dileep Mampallil ◽  
Dirk van den Ende ◽  
Frieder Mugele
Keyword(s):  
Surface Charge ◽  
Microfluidic Channels ◽  
Simple Method

Simple preparation method for Styrofoam–TiO2 composites and their photocatalytic application for dye oxidation and Cr(vi) reduction in industrial wastewater

2021 ◽  
Vol 7 (1) ◽  
pp. 222-230
Author(s):  
Youn-Jun Lee ◽  
Chang-Gu Lee ◽  
Jin-Kyu Kang ◽  
Seong-Jik Park ◽  
Pedro J. J. Alvarez
Keyword(s):  
Industrial Wastewater ◽  
Preparation Method ◽  
Expanded Polystyrene ◽  
Simple Method ◽  
Simple Preparation ◽  
Photocatalytic Application ◽  
Dye Oxidation

This study investigates a simple method for the preparation of floating photocatalysts in which the surface of expanded polystyrene (EPS) is partially dissolved using a diluted solvent that contains TiO2 particles.

scholarly journals Fabrication of Silicon Microfluidic Chips for Acoustic Particle Focusing Using Direct Laser Writing

Micromachines ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 113 ◽  
Author(s):  
Anna Fornell ◽  
Per Söderbäck ◽  
Zhenhua Liu ◽  
Milena De Albuquerque Moreira ◽  
Maria Tenje
Keyword(s):  
Acoustic Waves ◽  
Microfluidic Channel ◽  
Microfluidic Channels ◽  
Glass Wafer ◽  
Microfluidic Chips ◽  
Direct Laser Writing ◽  
Laser Writing ◽  
Simple Method ◽  
Particle Focusing ◽  
Direct Laser

We have developed a fast and simple method for fabricating microfluidic channels in silicon using direct laser writing. The laser microfabrication process was optimised to generate microfluidic channels with vertical walls suitable for acoustic particle focusing by bulk acoustic waves. The width of the acoustic resonance channel was designed to be 380 µm, branching into a trifurcation with 127 µm wide side outlet channels. The optimised settings used to make the microfluidic channels were 50% laser radiation power, 10 kHz pulse frequency and 35 passes. With these settings, six chips could be ablated in 5 h. The microfluidic channels were sealed with a glass wafer using adhesive bonding, diced into individual chips, and a piezoelectric transducer was glued to each chip. With acoustic actuation at 2.03 MHz a half wavelength resonance mode was generated in the microfluidic channel, and polystyrene microparticles (10 µm diameter) were focused along the centre-line of the channel. The presented fabrication process is especially interesting for research purposes as it opens up for rapid prototyping of silicon-glass microfluidic chips for acoustofluidic applications.

scholarly journals Simple Preparation of Diverse Neoagaro-Oligosaccharides

Processes ◽  
2019 ◽  
Vol 7 (5) ◽  
pp. 267 ◽  
Author(s):  
Fudi Lin ◽  
Jing Ye ◽  
Yayan Huang ◽  
Yucheng Yang ◽  
Meitian Xiao
Keyword(s):  
Activated Carbon ◽  
Column Chromatography ◽  
Substrate Concentration ◽  
Enzyme Hydrolysis ◽  
Simple Method ◽  
Nmr Structures ◽  
Simple Preparation ◽  
Authentic Standard

A simple method for obtaining pure and well-defined oligosaccharides was established by hydrolyzing agar with β-agarase from Vibrio natriegens. The conditions for enzymolysis were optimized as follows: a temperature of 45 °C, a pH of 8.5, a substrate concentration of 0.3%, an enzyme amount of 100 U/g and an enzymolysis time of 20 h. Neoagaro-oligosaccharides with different degrees of polymerization were obtained by hydrolyzing agar with β-agarase for different lengths of time. After removing pigments using activated carbon and salts by dialyzing, the enzyme hydrolysis solution was separated with Bio-Gel P2 column chromatography. Neoagaro-oligosaccharides with different degrees of polymerization were acquired. By comparing with authentic standard substances, along with further confirmation by FTIR, MS and NMR, structures of the purified neoagaro-oligosaccharides were identified as neoagarobiose (NA2), neoagaroteraose (NA4), neoagarohexaose (NA6), neoagarooctaose (NA8), neoagaro-decaose (NA10) and neoagarododecaose (NA12) with purities of more than 97.0%. The present study established a method for the preparation of various neoagaro-oligosaccharides that may be of great significance for further study of their bioactivities.

Simple preparation of magnetic field-responsive structural colored Janus particles

2018 ◽  
Vol 54 (21) ◽  
pp. 2607-2610 ◽  
Author(s):  
Midori Teshima ◽  
Takahiro Seki ◽  
Yukikazu Takeoka
Keyword(s):  
Magnetic Field ◽  
Magnetic Nanoparticles ◽  
Silica Particles ◽  
Janus Particles ◽  
Simple Method ◽  
Structural Colors ◽  
Simple Preparation

We established a simple method for preparing Janus particles displaying different structural colors using submicron-sized fine silica particles and magnetic nanoparticles composed of Fe3O4.

3D printing of liquid metals as fugitive inks for fabrication of 3D microfluidic channels

Lab on a Chip ◽  
2016 ◽  
Vol 16 (10) ◽  
pp. 1812-1820 ◽  
Author(s):  
Dishit P. Parekh ◽  
Collin Ladd ◽  
Lazar Panich ◽  
Khalil Moussa ◽  
Michael D. Dickey
Keyword(s):  
3D Printing ◽  
Liquid Metal ◽  
Room Temperature ◽  
Liquid Metals ◽  
Microfluidic Channels ◽  
Simple Method ◽  
Sacrificial Template

This paper demonstrates a simple method to fabricate 3D microchannels at room temperature by printing liquid metal as a sacrificial template.

A simple way for producing holographic filters suitable for image improvement

1978 ◽  
Vol 36 (1) ◽  
pp. 226-227
Author(s):  
K.-H. Herrmann ◽  
E. Reuber ◽  
P. Schiske
Keyword(s):  
Transfer Functions ◽  
Diffraction Order ◽  
Lateral Position ◽  
Simple Method ◽  
Spatial Frequencies ◽  
Resolution Electron ◽  
Wiener Filters ◽  
Image Improvement ◽  
Optical Reconstruction ◽  
Set Up

Aposteriori deblurring of high resolution electron micrographs of weak phase objects can be performed by holographic filters [1,2] which are arranged in the Fourier domain of a light-optical reconstruction set-up. According to the diffraction efficiency and the lateral position of the grating structure, the filters permit adjustment of the amplitudes and phases of the spatial frequencies in the image which is obtained in the first diffraction order.In the case of bright field imaging with axial illumination, the Contrast Transfer Functions (CTF) are oscillating, but real. For different imageforming conditions and several signal-to-noise ratios an extensive set of Wiener-filters should be available. A simple method of producing such filters by only photographic and mechanical means will be described here.A transparent master grating with 6.25 lines/mm and 160 mm diameter was produced by a high precision computer plotter. It is photographed through a rotating mask, plotted by a standard plotter.

Rotary Beveling for In Situ Thin-Section Microscopy of Cell Cultures

1981 ◽  
Vol 39 ◽  
pp. 550-551
Author(s):  
Dean A. Handley ◽  
Jack T. Alexander ◽  
Shu Chien
Keyword(s):  
Cell Growth ◽  
Cell Cultures ◽  
Molecular Interactions ◽  
Convenient Method ◽  
Petri Dish ◽  
Simple Method ◽  
Thin Section Microscopy ◽  
Thin Sectioning ◽  
Organic Fluids

In situ preparation of cell cultures for ultrastructural investigations is a convenient method by which fixation, dehydration and embedment are carried out in the culture petri dish. The in situ method offers the advantage of preserving the native orientation of cell-cell interactions, junctional regions and overlapping configurations. In order to section after embedment, the petri dish is usually separated from the polymerized resin by either differential cryo-contraction or solvation in organic fluids. The remaining resin block must be re-embedded before sectioning. Although removal of the petri dish may not disrupt the native cellular geometry, it does sacrifice what is now recognized as an important characteristic of cell growth: cell-substratum molecular interactions. To preserve the topographic cell-substratum relationship, we developed a simple method of tapered rotary beveling to reduce the petri dish thickness to a dimension suitable for direct thin sectioning.

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