A specific groove design for individualized healing in a canine partial sternal defect model by a polycaprolactone/hydroxyapatite scaffold coated with bone marrow stromal cells

2013 ◽  
Vol 102 (10) ◽  
pp. 3401-3408 ◽  
Author(s):  
Yiwen Xuan ◽  
Hua Tang ◽  
Bin Wu ◽  
Xinyu Ding ◽  
Zhongyuan Lu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Defect Model ◽  
Groove Design ◽  
Hydroxyapatite Scaffold

scholarly journals The Bone Regeneration Using Bone Marrow Stromal Cells with Moderate Concentration Platelet-Rich Plasma in Femoral Segmental Defect of Rats

2017 ◽  
Vol 11 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Junichi Yamakawa ◽  
Junichi Hashimoto ◽  
Mitsuo Takano ◽  
Michiaki Takagi
Keyword(s):  
Bone Marrow ◽  
Cell Proliferation ◽  
Bone Formation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Platelet Rich Plasma ◽  
Marrow Stromal Cells ◽  
Segmental Defect ◽  
Defect Model

Background: Platelet-rich plasma (PRP) can provide an assortment of growth factors, but how PRP effects bone regeneration is still unknown. The aim of the study was to explore an optimal method of using PRP and bone marrow stromal cells (BMSCs). Methods: An in vitro experiment was first conducted to determine an appropriate quantity of PRP. BMSCs were cultured with PRP of different concentrations to assess cell proliferation and osteogenic differentiation. Following the in vitro study, a rat femoral segmental defect model was used. Five collagen mixtures consisting of different concentrations of PRP and BMSCs were prepared as follows, i) BMSCs and PRP (platelet 20 x 104/µl), ii) BMSCs and PRP (platelet 100 x 104/µl), iii) BMSCs and PRP (platelet 500 x 104/µl), iv) BMSCs, and v) PRP group (platelet 100 x 104/µl), were used to fill defect. New bone formation was evaluated by soft X-ray and histologic analyses were performed at 2, 4, 6 and 8 weeks postoperatively. Results: The cell proliferation increased PRP concentration-dependently. Cellular alkaline phosphatase activity was higher in moderate concentration than high or low concentration group’s in vitro study. In vivo study, the bone fill percentage of newly formed bone in BMSCs and PRP (platelet 100 x 104/µl) was 46.9% at 8 weeks and increased significantly compared with other groups. Conclusion: BMSCs with moderate level of PRP significantly enhanced bone formation in comparison with BMSCs or PRP transplant in a rat femoral defect model.

Glucocorticoid exposure reduces expression of sclerostin in bone marrow stromal cells

2013 ◽  
Author(s):  
Sylvia Thiele ◽  
Alexander Rauch ◽  
Jan P Tuckermann ◽  
Lorenz C Hofbauer ◽  
Martina Rauner
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells
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