scholarly journals Preparation and Drug Release Properties of a Thermo Sensitive GA Hydrogel

Polymers ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 119
Author(s):  
Jiufang Duan ◽  
Yirong Huang ◽  
Shiyu Zong ◽  
Jianxin Jiang
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Release Rate ◽  
Bovine Serum ◽  
Early Stage ◽  
Drug Carrier ◽  
Order Equation ◽  
Solution Temperature ◽  
Swelling Kinetics ◽  
Static System

A high-strength galactomannan (GA)-based hydrogel with thermal response and pH response is introduced in this paper. GA, N-isopropylacrylamide (NIPAM), N-[3-dimethylamino)propyl]methylacrylamide (DMAPMA), and montmorillonite were used to form hydrogels through a simple mixed static system. Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), and scanning electron microscopy (SEM) were used to characterize the structure and properties of the hydrogels. The compressive strength of the the hydrogel increased from 23.9 to 105.61 kPa with the increase of GA dosage from 0 to 1.5 wt%. When the NIPAM content in the monomer increased from 75% to 95%, the lower critical solution temperature (LCST) of the hydrogel changed from 36.5 to 45.8 °C. When the monomer content was higher than 10wt%, the swelling kinetics of the sample changed from the second-order equation to the first-order equation. With the increase of the proportion of NIPAM monomer, the release rate of bovine serum album in the early stage was faster, and the cumulative release rate was close to 100%.The release rate of bovine serum albumin at 37 °C was higher than that at 25 °C. The release rate of the hydrogel containing bovine serum albumin was the fastest under the condition of pH 7.4, followed by those at pH 6.6 and pH 5.0. The results showed that this thermal-responsive hydrogel has potential applications as a drug carrier for colon delivery.

scholarly journals Studies on Thermal Aggregation of Bovine Serum Albumin as a Drug Carrier.

2000 ◽  
Vol 48 (4) ◽  
pp. 464-466 ◽  
Author(s):  
Chikako HONDA ◽  
Hiroko KAMIZONO ◽  
Tomomi SAMEJIMA ◽  
Kazutoyo ENDO
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Drug Carrier ◽  
Thermal Aggregation

PREPARATION OF FOLATE-CONJUGATED BOVINE SERUM ALBUMIN NANOPARTICLES ADSORBING EPIRUBICIN HYDROCHLORIDE

NANO ◽  
2013 ◽  
Vol 08 (06) ◽  
pp. 1350066 ◽  
Author(s):  
XIUHUA ZHAO ◽  
JIAMING TANG ◽  
YUANGANG ZU ◽  
WEIGUO WANG ◽  
NA LI ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Drug Carrier ◽  
Drug Loading ◽  
Carrier System ◽  
Adsorption Time ◽  
Inhibitory Rate ◽  
Albumin Nanoparticles ◽  
Drug Carrier System

This work investigated the preparation process of folate-conjugated bovine serum albumin nanoparticles (FA–BSANPs) adsorbing epirubicin hydrochloride (EPI) nanoparticles (FA–EPI–BSANPs), a specific-targeting drug delivery system in cancer chemotherapy. The BSANPs were prepared by desolvation as a drug carrier system and conjugated with folate to produce FA–EPI–BSANPs that specifically target tumors by cross-linking. EPI, an anticancer drug, was adsorbed by this drug carrier system. The influences of six experimental parameters, namely, the adsorption time, FA–BSANPs solution-adsorbed EPI concentration, stirring speed, FA–BSANPs solution pH, the ratio of glutaraldehyde and BSA, and mass ratio of FA–BSANPs to EPI, on the drug loading efficiency (DLR) and drug entrapment efficiency (DER) of FA–EPI–BSANPs were investigated via the single factor method. The results indicated that the optimum operation conditions were obtained with 145.4 nm±0.5 nm MPS, 23.41% DLR and 98.93% DER. The N -hydroxysuccinimide-folate content associated with BSANPs was up to 0.9757% (wt). The DLR and DER of EPI increased with increasing adsorption time, FA–BSANPs solution concentration, and pH value, peaking at 1750 rpm with increasing stirring speed, but decreasing thereafter. The FA–EPI–BSANPs obtained were characterized by laser light scattering, scanning electron microscopy, Fourier transform infrared spectroscopy, differential scanning calorimetry, X-ray diffraction and thermogravimetric analysis. Drug release in vitro was investigated, as well. The characterization results showed that EPI in FA–EPI–BSANPs existed in an amorphous, instead of crystalline state. Most of the EPI was enclosed by FA–BSANPs, and a small amount was adsorbed onto the surface of the FA–BSANPs. The FA–EPI–BSANPs particles are nearly ellipsoidal and significantly affect sustained release. The inhibitory rate of FA–EPI–BSANP was mensurated by MTT method. The inhibitory rate of FA–EPI–BSANPs for SMMC 7721 cell developed with raise of concentration and was higher than other samples. The IC50 values of FA–EPI–BSANPs and EPI were 11.5 μg/mL and 18.8 μg/mL, respectively. The target ability of FA–EPI–BSANP for SMMC 7721 cell was mensurated by fluorescence (FITC) modified albumin techniques. The uptake rate of FA–EPI–BSANPs was higher than samples without folate conjugated, and increased with increased concentration.

Bovine Serum Albumin as a Versatile Platform for Cancer Imaging and Therapy

2018 ◽  
Vol 25 (25) ◽  
pp. 2938-2953 ◽  
Author(s):  
Jun Wang ◽  
Bingbo Zhang
Keyword(s):  
Surface Modification ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Cancer Imaging ◽  
Drug Carrier ◽  
Drug Loading ◽  
Biomimetic Synthesis ◽  
Life Extension

Background: Due to the good biocompatibility, biodegradability, facile surface functionalization and high water solubility, Bovine serum albumin has gain increasing attention in the nanomedicine. Objective: Despite there are many reviews on albumin based nanoparticles, most of them focus on one aspect of the albumin functionality, e.g., drug delivery, cancer theranostics or half-life extension in vivo. This review aims to comprehensively summary bovine serum albumin as a versatile platform in the applications of cancer imaging and therapy. Methods: We review the extensive applications of bovine serum albumin in drug carrier, surface engineering and biomimetic synthesis for cancer imaging and therapy. Conclusion: Based on the studies reviewed, variety of in vitro and in vivo studies show good performance of bovine serum albumin as the drug carrier, surface modification agent and biomimetic template in cancer imaging and therapy. Nevertheless, there are still some issues to be solved, e.g., the technological parameters for enhancing the drug loading efficiency and controlling drug release, optimizing surface modification process to provide more stable nanoagents, investigation of the biomimetic mechanism, in-depth study of their toxicity, further exploring their bioapplications, etc.

Biomimetically Co-Deposition of Bovine Serum Albumin and Calcium Phosphate on 3D Printed Hydroxyapatite: Influence of Time, Temperature and Concentration

2018 ◽  
Vol 766 ◽  
pp. 83-87
Author(s):  
Faungchat Thammarakcharoen ◽  
Jintamai Suwanprateeb
Keyword(s):  
Phase Composition ◽  
Calcium Phosphate ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Weight Change ◽  
Bovine Serum ◽  
Deposition Process ◽  
Solution Temperature ◽  
Soaking Time ◽  
3D Printed

Influence of bovine serum albumin (BSA) concentration, soaking times and soaking temperatures during biomimetic co-deposition process of three dimensionally printed hydroxyapatite (3D printed HA) on weight change, phase composition and microstructure were studied. At 23°C, weight change of all samples monotonically increased with increasing soaking time regardless of BSA concentration used while the weight change initially increased, but leveled off later with increasing soaking time when using solution temperature of 37°C. At 50°C, weight change initially increased for up to 4 h and remained constant for up to 16 h where the weight decreased afterward. This decrease could be related to the competition between the dissolution of 3D printed HA, the deposition of new calcium phosphate crystals and the incorporation of BSA during biomimetic process at high temperatures. From X-ray diffraction, no influence in BSA concentration, time and temperature on the phase composition was observed. Scanning electron microscopy revealed the deposition of new plate-like calcium phosphate crystals on all 3D printed HA. Hydroxyapatite and octacalcium phosphate were found to be the main phases of all biomimetically BSA.

Bovine Serum Albumin Modified Mg-Al Layered Double Oxides Nanohybrids: Preparation and Potential Application as Drug Carrier

2011 ◽  
Vol 396-398 ◽  
pp. 330-335
Author(s):  
Ting Fan ◽  
Hui Zhang ◽  
Shao Huan Guo
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Physicochemical Properties ◽  
Bovine Serum ◽  
Drug Carrier ◽  
Double Oxide ◽  
Adsorption Behaviors ◽  
Structure Morphology ◽  
Model Protein ◽  
Ft Ir

Biocompatible MgAl-CO3-LDH-derived layered double oxide (LDO) as the host and bovine serum albumin (BSA) as a model protein have been chosen to fabricate a novel bioinorganic nanohybrid (BSA-LDO). The structure, morphology and physicochemical properties of the obtained nanohybrids are investigated by XRD, FT-IR, SEM and UV-vis methods and the interaction between LDO and BSA molecules has been discussed. The application of these nanohybrids as drug carrier is primarily studied by examining the adsorption behaviors of three drugs.

scholarly journals FTIR Characterization and Release of Bovine Serum Albumin from Bioactive Glasses

2017 ◽  
Vol 15 (4) ◽  
pp. e347-e355 ◽  
Author(s):  
Wai-Ching Liu ◽  
Blake Ballenger ◽  
Amnah Algarni ◽  
Mariano Velez ◽  
Tien-Min G. Chu
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bioactive Glass ◽  
Bovine Serum ◽  
Drug Carrier ◽  
Helical Structure ◽  
Burst Release ◽  
Chitosan Coating ◽  
Degradation Rates ◽  
Percent Protein

Background Bioactive glass has attracted substantial interest in orthopedics, but it has been less explored as a drug carrier. This study investigated the bovine serum albumin (BSA) release from bioactive 13–93B0 and 13–93B3 glasses. Methods Glass disks (13–93B0 and 13–93B3; n = 5) were loaded with 4 mg of BSA and coated under different chitosan-coating conditions. The amount of BSA released in phosphate-buffered saline (PBS) was evaluated, and a degradation study was performed to find out the weight loss and pH of PBS. Secondary structures of BSA on 13–93B0 were characterized by Fourier transform infrared (FTIR) spectroscopy. Results One hundred percent protein release occurred by 24 hours for all 13–93B3 groups. However, chitosan coating delayed 100% release up to 72 hours in 13–93B0 groups. The 13–93B3 glass showed higher degradation rates than 13–93B0 regardless of chitosan-coating status. Multilayer and sandwich chitosan coatings further delayed BSA release from 13–93B0. FTIR analysis revealed that α-helical structure was the highest among all groups and significantly higher in the 2% sandwich chitosan coating group (32.0% ± 2.1%), compared with uncoated and 4% chitosan groups. Conclusions Chitosan coating can delay the burst release of BSA from 13–93B0 glass and be a potential coating on bioactive glass for drug delivery purposes.

TPGS modified reduced bovine serum albumin nanoparticles as a lipophilic anticancer drug carrier for overcoming multidrug resistance

2016 ◽  
Vol 4 (22) ◽  
pp. 3959-3968 ◽  
Author(s):  
Fangcheng Chen ◽  
Jun Wu ◽  
Chunli Zheng ◽  
Jiabi Zhu ◽  
Yajie Zhang ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Anticancer Drug ◽  
Bovine Serum ◽  
Drug Carrier ◽  
Glycol Succinate ◽  
Anticancer Drug Delivery ◽  
Protein Polymer ◽  
Polymer Conjugate ◽  
Novel Protein

In this study, a novel protein–polymer conjugate, d-α-tocopheryl polyethylene glycol succinate modified reduced bovine serum albumin (TPGS–Re-BSA, TRB), was synthesized for lipophilic anticancer drug delivery, and its unique ability to overcome drug resistance was explored.

Improvement of an artificial test substrate technique for evaluation of em immunocytochemical labeling

1987 ◽  
Vol 45 ◽  
pp. 762-763
Author(s):  
G. D. Gagne ◽  
M. F. Miller
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Artificial Substrate ◽  
Chemical Fixation ◽  
As If

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.

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