scholarly journals Studies on Thermal Aggregation of Bovine Serum Albumin as a Drug Carrier.

2000 ◽  
Vol 48 (4) ◽  
pp. 464-466 ◽  
Author(s):  
Chikako HONDA ◽  
Hiroko KAMIZONO ◽  
Tomomi SAMEJIMA ◽  
Kazutoyo ENDO
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Drug Carrier ◽  
Thermal Aggregation

Influence of metal ions on thermal aggregation of bovine serum albumin: Aggregation kinetics and structural changes

2009 ◽  
Vol 103 (12) ◽  
pp. 1729-1738 ◽  
Author(s):  
Giovanna Navarra ◽  
Anna Tinti ◽  
Maurizio Leone ◽  
Valeria Militello ◽  
Armida Torreggiani
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Metal Ions ◽  
Bovine Serum ◽  
Structural Changes ◽  
Aggregation Kinetics ◽  
Thermal Aggregation

Thermal Aggregation of Bovine Serum Albumin in Trehalose and Sucrose Aqueous Solutions

2012 ◽  
Vol 116 (39) ◽  
pp. 11829-11836 ◽  
Author(s):  
Massimo Panzica ◽  
Antonio Emanuele ◽  
Lorenzo Cordone
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Aqueous Solutions ◽  
Bovine Serum ◽  
Thermal Aggregation

PREPARATION OF FOLATE-CONJUGATED BOVINE SERUM ALBUMIN NANOPARTICLES ADSORBING EPIRUBICIN HYDROCHLORIDE

NANO ◽  
2013 ◽  
Vol 08 (06) ◽  
pp. 1350066 ◽  
Author(s):  
XIUHUA ZHAO ◽  
JIAMING TANG ◽  
YUANGANG ZU ◽  
WEIGUO WANG ◽  
NA LI ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Drug Carrier ◽  
Drug Loading ◽  
Carrier System ◽  
Adsorption Time ◽  
Inhibitory Rate ◽  
Albumin Nanoparticles ◽  
Drug Carrier System

This work investigated the preparation process of folate-conjugated bovine serum albumin nanoparticles (FA–BSANPs) adsorbing epirubicin hydrochloride (EPI) nanoparticles (FA–EPI–BSANPs), a specific-targeting drug delivery system in cancer chemotherapy. The BSANPs were prepared by desolvation as a drug carrier system and conjugated with folate to produce FA–EPI–BSANPs that specifically target tumors by cross-linking. EPI, an anticancer drug, was adsorbed by this drug carrier system. The influences of six experimental parameters, namely, the adsorption time, FA–BSANPs solution-adsorbed EPI concentration, stirring speed, FA–BSANPs solution pH, the ratio of glutaraldehyde and BSA, and mass ratio of FA–BSANPs to EPI, on the drug loading efficiency (DLR) and drug entrapment efficiency (DER) of FA–EPI–BSANPs were investigated via the single factor method. The results indicated that the optimum operation conditions were obtained with 145.4 nm±0.5 nm MPS, 23.41% DLR and 98.93% DER. The N -hydroxysuccinimide-folate content associated with BSANPs was up to 0.9757% (wt). The DLR and DER of EPI increased with increasing adsorption time, FA–BSANPs solution concentration, and pH value, peaking at 1750 rpm with increasing stirring speed, but decreasing thereafter. The FA–EPI–BSANPs obtained were characterized by laser light scattering, scanning electron microscopy, Fourier transform infrared spectroscopy, differential scanning calorimetry, X-ray diffraction and thermogravimetric analysis. Drug release in vitro was investigated, as well. The characterization results showed that EPI in FA–EPI–BSANPs existed in an amorphous, instead of crystalline state. Most of the EPI was enclosed by FA–BSANPs, and a small amount was adsorbed onto the surface of the FA–BSANPs. The FA–EPI–BSANPs particles are nearly ellipsoidal and significantly affect sustained release. The inhibitory rate of FA–EPI–BSANP was mensurated by MTT method. The inhibitory rate of FA–EPI–BSANPs for SMMC 7721 cell developed with raise of concentration and was higher than other samples. The IC50 values of FA–EPI–BSANPs and EPI were 11.5 μg/mL and 18.8 μg/mL, respectively. The target ability of FA–EPI–BSANP for SMMC 7721 cell was mensurated by fluorescence (FITC) modified albumin techniques. The uptake rate of FA–EPI–BSANPs was higher than samples without folate conjugated, and increased with increased concentration.

Thermal aggregation of glycated bovine serum albumin

2010 ◽  
Vol 1804 (4) ◽  
pp. 789-798 ◽  
Author(s):  
Philippe Rondeau ◽  
Giovanna Navarra ◽  
Francesco Cacciabaudo ◽  
Maurizio Leone ◽  
Emmanuel Bourdon ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Thermal Aggregation

scholarly journals Preparation and Drug Release Properties of a Thermo Sensitive GA Hydrogel

Polymers ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 119
Author(s):  
Jiufang Duan ◽  
Yirong Huang ◽  
Shiyu Zong ◽  
Jianxin Jiang
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Release Rate ◽  
Bovine Serum ◽  
Early Stage ◽  
Drug Carrier ◽  
Order Equation ◽  
Solution Temperature ◽  
Swelling Kinetics ◽  
Static System

A high-strength galactomannan (GA)-based hydrogel with thermal response and pH response is introduced in this paper. GA, N-isopropylacrylamide (NIPAM), N-[3-dimethylamino)propyl]methylacrylamide (DMAPMA), and montmorillonite were used to form hydrogels through a simple mixed static system. Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), and scanning electron microscopy (SEM) were used to characterize the structure and properties of the hydrogels. The compressive strength of the the hydrogel increased from 23.9 to 105.61 kPa with the increase of GA dosage from 0 to 1.5 wt%. When the NIPAM content in the monomer increased from 75% to 95%, the lower critical solution temperature (LCST) of the hydrogel changed from 36.5 to 45.8 °C. When the monomer content was higher than 10wt%, the swelling kinetics of the sample changed from the second-order equation to the first-order equation. With the increase of the proportion of NIPAM monomer, the release rate of bovine serum album in the early stage was faster, and the cumulative release rate was close to 100%.The release rate of bovine serum albumin at 37 °C was higher than that at 25 °C. The release rate of the hydrogel containing bovine serum albumin was the fastest under the condition of pH 7.4, followed by those at pH 6.6 and pH 5.0. The results showed that this thermal-responsive hydrogel has potential applications as a drug carrier for colon delivery.

Thermal aggregation and ion-induced cold-gelation of bovine serum albumin

2009 ◽  
Vol 38 (4) ◽  
pp. 437-446 ◽  
Author(s):  
Giovanna Navarra ◽  
Daniela Giacomazza ◽  
Maurizio Leone ◽  
Fabio Librizzi ◽  
Valeria Militello ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Thermal Aggregation

Thermal Aggregation of Bovine Serum Albumin in Conventional Buffers: An Insight into Molecular Level Interactions

2017 ◽  
Vol 46 (4) ◽  
pp. 831-848 ◽  
Author(s):  
Lakkoji Satish ◽  
Sabera Millan ◽  
Satyabrata Das ◽  
Sarita Jena ◽  
Harekrushna Sahoo
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Molecular Level ◽  
Thermal Aggregation ◽  
Insight Into

Bovine Serum Albumin as a Versatile Platform for Cancer Imaging and Therapy

2018 ◽  
Vol 25 (25) ◽  
pp. 2938-2953 ◽  
Author(s):  
Jun Wang ◽  
Bingbo Zhang
Keyword(s):  
Surface Modification ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Cancer Imaging ◽  
Drug Carrier ◽  
Drug Loading ◽  
Biomimetic Synthesis ◽  
Life Extension

Background: Due to the good biocompatibility, biodegradability, facile surface functionalization and high water solubility, Bovine serum albumin has gain increasing attention in the nanomedicine. Objective: Despite there are many reviews on albumin based nanoparticles, most of them focus on one aspect of the albumin functionality, e.g., drug delivery, cancer theranostics or half-life extension in vivo. This review aims to comprehensively summary bovine serum albumin as a versatile platform in the applications of cancer imaging and therapy. Methods: We review the extensive applications of bovine serum albumin in drug carrier, surface engineering and biomimetic synthesis for cancer imaging and therapy. Conclusion: Based on the studies reviewed, variety of in vitro and in vivo studies show good performance of bovine serum albumin as the drug carrier, surface modification agent and biomimetic template in cancer imaging and therapy. Nevertheless, there are still some issues to be solved, e.g., the technological parameters for enhancing the drug loading efficiency and controlling drug release, optimizing surface modification process to provide more stable nanoagents, investigation of the biomimetic mechanism, in-depth study of their toxicity, further exploring their bioapplications, etc.

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