scholarly journals Aligned Nanofiber-Guided Bone Regeneration Barrier Incorporated with Equine Bone-Derived Hydroxyapatite for Alveolar Bone Regeneration

Polymers ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 60
Author(s):  
Jae Woon Lim ◽  
Kyoung-Je Jang ◽  
Hyunmok Son ◽  
Sangbae Park ◽  
Jae Eun Kim ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Tissue ◽  
High Speed ◽  
Alveolar Bone ◽  
Guided Bone Regeneration ◽  
Bone Tissue Regeneration ◽  
Calcium Deposition ◽  
Graft Material ◽  
Post Surgery ◽  
Equine Bone

Post-surgery failure of dental implants due to alveolar bone loss is currently critical, disturbing the quality of life of senior dental patients. To overcome this problem, bioceramic or bone graft material is loaded into the defect. However, connective tissue invasion instead of osteogenic tissue limits bone tissue regeneration. The guided bone regeneration concept was adapted to solve this problem and still has room for improvements, such as biochemical similarity or oriented structure. In this article, an aligned electrospun-guided bone regeneration barrier with xenograft equine bone-derived nano hydroxyapatite (EBNH-RB) was fabricated by electrospinning EBNH/PCL solution on high-speed rotating drum collector and fiber characterization, viability and differentiation enhancing properties of mesenchymal dental pulp stem cell on the barrier was determined. EBNH-RB showed biochemical and structural similarity to natural bone tissue electron microscopy image analysis and x-ray diffractometer analysis, and had a significantly better effect in promoting osteogenesis based on the increased bioceramic content by promoting cell viability, calcium deposition and osteogenic marker expression, suggesting that they can be successfully applied to regenerate alveolar bone as a guided bone regeneration barrier.

scholarly journals Guided Bone Regeneration- A Comprehensive Review

2021 ◽  
Author(s):  
Vineetha Venugopalan ◽  
Anegundi Raghavendra Vamsi ◽  
Santhosh Shenoy ◽  
Karishma Ashok ◽  
Biju Thomas
Keyword(s):  
Bone Regeneration ◽  
Alveolar Bone ◽  
Primary Stability ◽  
Guided Bone Regeneration ◽  
Collagen Membrane ◽  
Bony Defect ◽  
Graft Material ◽  
Bone Augmentation ◽  
Wide Range ◽  
Space Maintenance

Successful implant treatment requires prosthetically driven placement of an implant, primary stability at placement, and careful living bone management. The resorptive changes of alveolar bone are an inevitable process following tooth loss, periodontal disease or trauma which causes bone defects. This results in various aesthetic and functional complications such as soft tissue recession, infection and inflammation. Various methods have been tried and advocated for augmenting these bone deficiencies. Guided Bone Regeneration (GBR) is a successful modality for bone augmentation with a wide range of indications and helps restore the alveolar ridge dimensions. It utilises the principle of Guided Tissue Regeneration (GTR) for space maintenance within a bony defect. Different types of barrier membranes are being utilised along with various bone grafts in GBR. Thorough knowledge regarding the biology of bone is required before the initiation of any bone augmentation procedure. A combination of Collagen Membrane (CM) and graft material was found successful for GBR. Hence, this review focuses on presentation of best available evidence for various aspects of GBR.

scholarly journals Chitosan-Human Bone Composite Granulates for Guided Bone Regeneration

2021 ◽  
Vol 22 (5) ◽  
pp. 2324
Author(s):  
Piotr Kowalczyk ◽  
Rafał Podgórski ◽  
Michał Wojasiński ◽  
Grzegorz Gut ◽  
Witold Bojar ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Tissue ◽  
Guided Bone Regeneration ◽  
Tissue Growth ◽  
Human Bone ◽  
Additional Stress ◽  
Tissue Banks ◽  
Graft Material ◽  
Bone Harvesting

The search for the perfect bone graft material is an important topic in material science and medicine. Despite human bone being the ideal material, due to its composition, morphology, and familiarity with cells, autografts are widely considered demanding and cause additional stress to the patient because of bone harvesting. However, human bone from tissue banks can be used to prepare materials in eligible form for transplantation. Without proteins and fats, the bone becomes a non-immunogenic matrix for human cells to repopulate in the place of implantation. To repair bone losses, the granulate form of the material is easy to apply and forms an interconnected porous structure. A granulate composed of β-tricalcium phosphate, pulverized human bone, and chitosan—a potent biopolymer applied in tissue engineering, regenerative medicine, and biotechnology—has been developed. A commercial encapsulator was used to obtain granulate, using chitosan gelation upon pH increase. The granulate has been proven in vitro to be non-cytotoxic, suitable for MG63 cell growth on its surface, and increasing alkaline phosphatase activity, an important biological marker of bone tissue growth. Moreover, the granulate is suitable for thermal sterilization without losing its form—increasing its convenience for application in surgery for guided bone regeneration in case of minor or non-load bearing voids in bone tissue.

scholarly journals Human Teeth-Derived Bioceramics for Improved Bone Regeneration

Nanomaterials ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2396
Author(s):  
Ki-Taek Lim ◽  
Dinesh K. Patel ◽  
Sayan Deb Dutta ◽  
Han-Wool Choung ◽  
Hexiu Jin ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Tissue ◽  
Inductively Coupled Plasma ◽  
Alveolar Bone ◽  
Bone Tissue Regeneration ◽  
Molar Ratio ◽  
Human Teeth ◽  
Inductively Coupled ◽  
Plasma Mass Spectrometry ◽  
Xrd Patterns

Hydroxyapatite (HAp, Ca10(PO4)6(OH)2) is one of the most promising candidates of the calcium phosphate family, suitable for bone tissue regeneration due to its structural similarities with human hard tissues. However, the requirements of high purity and the non-availability of adequate synthetic techniques limit the application of synthetic HAp in bone tissue engineering. Herein, we developed and evaluated the bone regeneration potential of human teeth-derived bioceramics in mice′s defective skulls. The developed bioceramics were analyzed by X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, and scanning electron microscopy (FE-SEM). The developed bioceramics exhibited the characteristic peaks of HAp in FTIR and XRD patterns. The inductively coupled plasma mass spectrometry (ICP-MS) technique was applied to determine the Ca/P molar ratio in the developed bioceramics, and it was 1.67. Cytotoxicity of the simulated body fluid (SBF)-soaked bioceramics was evaluated by WST-1 assay in the presence of human alveolar bone marrow stem cells (hABMSCs). No adverse effects were observed in the presence of the developed bioceramics, indicating their biocompatibility. The cells adequately adhered to the bioceramics-treated media. Enhanced bone regeneration occurred in the presence of the developed bioceramics in the defected skulls of mice, and this potential was profoundly affected by the size of the developed bioceramics. The bioceramics-treated mice groups exhibited greater vascularization compared to control. Therefore, the developed bioceramics have the potential to be used as biomaterials for bone regeneration application.

scholarly journals Hyaluronic acid as a bioactive component for bone tissue regeneration: Fabrication, modification, properties, and biological functions

2020 ◽  
Vol 9 (1) ◽  
pp. 1059-1079
Author(s):  
Fei Xing ◽  
Changchun Zhou ◽  
Didi Hui ◽  
Colin Du ◽  
Lina Wu ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Wound Repair ◽  
Bone Tissue Regeneration ◽  
Biological Functions ◽  
Bioactive Polymers ◽  
Bioactive Component ◽  
Good Potential ◽  
Processing Properties

AbstractHyaluronic acid (HA) is widely distributed in the human body, and it is heavily involved in many physiological functions such as tissue hydration, wound repair, and cell migration. In recent years, HA and its derivatives have been widely used as advanced bioactive polymers for bone regeneration. Many medical products containing HA have been developed because this natural polymer has been proven to be nontoxic, noninflammatory, biodegradable, and biocompatible. Moreover, HA-based composite scaffolds have shown good potential for promoting osteogenesis and mineralization. Recently, many HA-based biomaterials have been fabricated for bone regeneration by combining with electrospinning and 3D printing technology. In this review, the polymer structures, processing, properties, and applications in bone tissue engineering are summarized. The challenges and prospects of HA polymers are also discussed.

scholarly journals Horizontal-guided Bone Regeneration using a Titanium Mesh and an Equine Bone Graft

2015 ◽  
Vol 16 (2) ◽  
pp. 154-162 ◽  
Author(s):  
Danilo Alessio Di Stefano ◽  
Gian Battista Greco ◽  
Lorenzo Cinci ◽  
Laura Pieri
Keyword(s):  
Bone Graft ◽  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
Bone Sample ◽  
Successful Outcome ◽  
Autogenous Bone ◽  
Titanium Mesh ◽  
Ridge Augmentation ◽  
Stereolithographic Model ◽  
Equine Bone

ABSTRACT Aim The present work describes a horizontal ridge augmentation in which a titanium mesh was preshaped by adapting it to a stereolithographic model of the patient's jaw that was fabricated from CT scans. Background Guided bone regeneration (GBR) involves covering the augmentation site with a long-lasting barrier to protect it from the invasion of surrounding soft tissues. Among barriers, titanium meshes may provide a successful outcome, but the intraoperatory time needed to shape them is a disadvantage. Case description The 54-year-old patient, missing the right mandibular second bicuspid, first molar, and second molar, had her atrophic ridge augmented with a 30:70 mixture of autogenous bone and equine, enzyme-deantigenic collagenpreserved bone substitute. Two conical implants were inserted concomitantly in the second bicuspid and first molar positions, and the site was protected with the preshaped mesh. Four months later, the titanium mesh was retrieved, a bone sample was collected, and histological and histomorphometric analyses were performed. Provisional and definitive prostheses were then delivered, and follow-up controls were performed for up to 24 months. Conclusion Preshaping the mesh on a model of the patient's mandible shortened the surgical time and enabled faster mesh placement. Two years after surgery, the implants were perfectly functional, and the bone width was stable over time as shown by radiographic controls. Histological analysis of the bone sample showed the heterologous biomaterial to be biocompatible and undergoing advanced remodeling and replacement with newly formed bone. Clinical significance Preshaping a titanium mesh over a stereolithographic model of the patient's jaw allowed for a significant reduction of the intraoperative time and may be therefore, advisable in routine practice. How to cite this article Di Stefano DA, Greco GB, Cinci L, Pieri L. Horizontal-guided Bone Regeneration using a Titanium Mesh and an Equine Bone Graft. J Contemp Dent Pract 2015;16(2):154-162.

scholarly journals Bone Regeneration Using a Three-Dimensional Hexahedron Channeled BCP Block Combined with Bone Morphogenic Protein-2 in Rat Calvarial Defects

Materials ◽  
2019 ◽  
Vol 12 (15) ◽  
pp. 2435 ◽  
Author(s):  
So-Yeun Kim ◽  
Eun-Bin Bae ◽  
Jae-Woong Huh ◽  
Jong-Ju Ahn ◽  
Hyun-Young Bae ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Alveolar Bone ◽  
Three Dimensional ◽  
Bone Morphogenetic Protein 2 ◽  
Graft Material ◽  
Implant Placement ◽  
Morphogenetic Protein ◽  
Human Bone Morphogenetic Protein ◽  
Calvarial Defects ◽  
Rat Calvarial Defect

It is important to obtain sufficient bone mass before implant placement on alveolar bone, and synthetic bone such as biphasic calcium phosphate (BCP) has been studied to secure this. This study used a BCP block bone with a specific structure of the three-dimensional (3D) hexahedron channel and coating with recombinant human bone morphogenetic protein-2 (rhBMP-2) impregnated carboxymethyl cellulose (CMC) was used to examine the enhancement of bone regeneration of this biomaterial in rat calvarial defect. After the preparation of critical-size calvarial defects in fifteen rats, defects were divided into three groups and were implanted with the assigned specimen (n = 5): Boneplant (untreated 3D hexahedron channeled BCP block), Boneplant/CMC (3D hexahedron channeled BCP block coated with CMC), and Boneplant/CMC/BMP (3D hexahedron channeled BCP block coated with CMC containing rhBMP-2). After 4 weeks, the volumetric, histologic, and histometric analyses were conducted to measure the newly formed bone. Histologically, defects in the Boneplant/CMC/BMP group were almost completely filled with new bone compared to the Boneplant and Boneplant/CMC groups. The new bone volume (P < 0.05) and area (P < 0.001) in the Boneplant/CMC/BMP group (20.12% ± 2.17, 33.79% ± 3.66) were much greater than those in the Boneplant (10.77% ± 4.8, 16.48% ± 9.11) and Boneplant/CMC (10.72% ± 3.29, 16.57% ± 8.94) groups, respectively. In conclusion, the 3D hexahedron channeled BCP block adapted rhBMP-2 with carrier CMC showed high possibility as an effective bone graft material.

scholarly journals Current Stage of Marine Ceramic Grafts for 3D Bone Tissue Regeneration

Marine Drugs ◽  
2019 ◽  
Vol 17 (8) ◽  
pp. 471 ◽  
Author(s):  
Patricia Diaz-Rodriguez ◽  
Miriam López-Álvarez ◽  
Julia Serra ◽  
Pío González ◽  
Mariana Landín
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Alveolar Bone ◽  
3D Cell Culture ◽  
Spinal Fractures ◽  
Bone Grafts ◽  
Bone Tissue Regeneration ◽  
Marine Origin ◽  
Age Related ◽  
Current Stage

Bioceramic scaffolds are crucial in tissue engineering for bone regeneration. They usually provide hierarchical porosity, bioactivity, and mechanical support supplying osteoconductive properties and allowing for 3D cell culture. In the case of age-related diseases such as osteoarthritis and osteoporosis, or other bone alterations as alveolar bone resorption or spinal fractures, functional tissue recovery usually requires the use of grafts. These bone grafts or bone void fillers are usually based on porous calcium phosphate grains which, once disposed into the bone defect, act as scaffolds by incorporating, to their own porosity, the intergranular one. Despite their routine use in traumatology and dental applications, specific graft requirements such as osteoinductivity or balanced dissolution rate are still not completely fulfilled. Marine origin bioceramics research opens the possibility to find new sources of bone grafts given the wide diversity of marine materials still largely unexplored. The interest in this field has also been urged by the limitations of synthetic or mammalian-derived grafts already in use and broadly investigated. The present review covers the current stage of major marine origin bioceramic grafts for bone tissue regeneration and their promising properties. Both products already available on the market and those in preclinical phases are included. To understand their clear contribution to the field, the main clinical requirements and the current available biological-derived ceramic grafts with their advantages and limitations have been collected.

scholarly journals Biomimetic and osteogenic 3D silk fibroin composite scaffolds with nano MgO and mineralized hydroxyapatite for bone regeneration

2020 ◽  
Vol 11 ◽  
pp. 204173142096779
Author(s):  
Ziquan Wu ◽  
Zhulong Meng ◽  
Qianjin Wu ◽  
Delu Zeng ◽  
Zhengdong Guo ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Tissue ◽  
Silk Fibroin ◽  
Bone Repair ◽  
Composite Scaffold ◽  
Bone Tissue Regeneration ◽  
Bone Mesenchymal Stem Cells ◽  
Differentiation Potential ◽  
Composite Effect ◽  
Sd Rat

Artificial bioactive materials have received increasing attention worldwide in clinical orthopedics to repair bone defects that are caused by trauma, infections or tumors, especially dedicated to the multifunctional composite effect of materials. In this study, a weakly alkaline, biomimetic and osteogenic, three-dimensional composite scaffold (3DS) with hydroxyapatite (HAp) and nano magnesium oxide (MgO) embedded in fiber (F) of silkworm cocoon and silk fibroin (SF) is evaluated comprehensively for its bone repair potential in vivo and in vitro experiments, particularly focusing on the combined effect between HAp and MgO. Magnesium ions (Mg2+) has long been proven to promote bone tissue regeneration, and HAp is provided with osteoconductive properties. Interestingly, the weak alkaline microenvironment from MgO may also be crucial to promote Sprague-Dawley (SD) rat bone mesenchymal stem cells (BMSCs) proliferation, osteogenic differentiation and alkaline phosphatase (ALP) activities. This SF/F/HAp/nano MgO (SFFHM) 3DS with superior biocompatibility and biodegradability has better mechanical properties, BMSCs proliferation ability, osteogenic activity and differentiation potential compared with the scaffolds adding HAp or MgO alone or neither. Similarly, corresponding meaningful results are also demonstrated in a model of distal lateral femoral defect in SD rat. Therefore, we provide a promising 3D composite scaffold for promoting bone regeneration applications in bone tissue engineering.

Guided Bone Regeneration of Femoral Segmental Defects using Equine Bone Graft: An In-Vivo Micro-Computed Tomographic Study in Rats

2018 ◽  
Vol 32 (5) ◽  
pp. 456-466 ◽  
Author(s):  
Mohammed Awadh Binsalah ◽  
Sundar Ramalingam ◽  
Mohammed Alkindi ◽  
Nasser Nooh ◽  
Khalid Al-Hezaimi
Keyword(s):  
Bone Graft ◽  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
Computed Tomographic ◽  
Equine Bone

scholarly journals Bone Regeneration in Critical-Sized Bone Defects Treated with Additively Manufactured Porous Metallic Biomaterials: The Effects of Inelastic Mechanical Properties

Materials ◽  
2020 ◽  
Vol 13 (8) ◽  
pp. 1992
Author(s):  
Marianne Koolen ◽  
Saber Amin Yavari ◽  
Karel Lietaert ◽  
Ruben Wauthle ◽  
Amir A. Zadpoor ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bulk Material ◽  
Bone Tissue Regeneration ◽  
Bony Tissue ◽  
Metallic Biomaterials ◽  
Cp Ti ◽  
Porous Biomaterials

Additively manufactured (AM) porous metallic biomaterials, in general, and AM porous titanium, in particular, have recently emerged as promising candidates for bone substitution. The porous design of such materials allows for mimicking the elastic mechanical properties of native bone tissue and showed to be effective in improving bone regeneration. It is, however, not clear what role the other mechanical properties of the bulk material such as ductility play in the performance of such biomaterials. In this study, we compared the bone tissue regeneration performance of AM porous biomaterials made from the commonly used titanium alloy Ti6Al4V-ELI with that of commercially pure titanium (CP-Ti). CP-Ti was selected because of its high ductility as compared to Ti6Al4V-ELI. Critical-sized (6 mm diameter) femoral defects in rats were treated with implants made from both Ti6Al4V-ELI and CP-Ti. Bone regeneration was assessed up to 11 weeks using micro-CT scanning. The regenerated bone volume was assessed ex vivo followed by histology and biomechanical testing to assess osseointegration of the implants. The bony defects treated with AM CP-Ti implants generally showed higher volumes of regenerated bone as compared to those treated with AM Ti6Al4V-ELI. The torsional strength of the two titanium groups were similar however, and both considerably lower than those measured for intact bony tissue. These findings show the importance of material type and ductility of the bulk material in the ability for bone tissue regeneration of AM porous biomaterials.

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