scholarly journals Human Teeth-Derived Bioceramics for Improved Bone Regeneration

Nanomaterials ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2396
Author(s):  
Ki-Taek Lim ◽  
Dinesh K. Patel ◽  
Sayan Deb Dutta ◽  
Han-Wool Choung ◽  
Hexiu Jin ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Tissue ◽  
Inductively Coupled Plasma ◽  
Alveolar Bone ◽  
Bone Tissue Regeneration ◽  
Molar Ratio ◽  
Human Teeth ◽  
Inductively Coupled ◽  
Plasma Mass Spectrometry ◽  
Xrd Patterns

Hydroxyapatite (HAp, Ca10(PO4)6(OH)2) is one of the most promising candidates of the calcium phosphate family, suitable for bone tissue regeneration due to its structural similarities with human hard tissues. However, the requirements of high purity and the non-availability of adequate synthetic techniques limit the application of synthetic HAp in bone tissue engineering. Herein, we developed and evaluated the bone regeneration potential of human teeth-derived bioceramics in mice′s defective skulls. The developed bioceramics were analyzed by X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, and scanning electron microscopy (FE-SEM). The developed bioceramics exhibited the characteristic peaks of HAp in FTIR and XRD patterns. The inductively coupled plasma mass spectrometry (ICP-MS) technique was applied to determine the Ca/P molar ratio in the developed bioceramics, and it was 1.67. Cytotoxicity of the simulated body fluid (SBF)-soaked bioceramics was evaluated by WST-1 assay in the presence of human alveolar bone marrow stem cells (hABMSCs). No adverse effects were observed in the presence of the developed bioceramics, indicating their biocompatibility. The cells adequately adhered to the bioceramics-treated media. Enhanced bone regeneration occurred in the presence of the developed bioceramics in the defected skulls of mice, and this potential was profoundly affected by the size of the developed bioceramics. The bioceramics-treated mice groups exhibited greater vascularization compared to control. Therefore, the developed bioceramics have the potential to be used as biomaterials for bone regeneration application.

Improved Deposition Process of CVD-(Ba,Sr)TiO3 on Ru

2000 ◽  
Vol 655 ◽  
Author(s):  
M. Tarutani ◽  
T. Sato ◽  
M. Yamamuka ◽  
T. Kawahara ◽  
T. Horikawa ◽  
...  
Keyword(s):  
Electrical Properties ◽  
Inductively Coupled Plasma ◽  
Deposition Process ◽  
Molar Ratio ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry ◽  
Bst Films ◽  
20 Nm ◽  
Uniform Composition

Abstract(Ba,Sr)TiO3 [BST] films were deposited by the flash vaporization CVD method with a unique liquid delivery system. An inductively coupled plasma mass spectrometry [ICP-MS] analysis revealed the decline of (Ba+Sr)/Ti molar ratio of the initial BST-layer on Ru. By readjusting the flow ratio of liquid sources and using a two-step deposition method, we obtained 30-nm-thick BST films with uniform composition profile, exhibiting good electrical properties. The leakage property, however, was severely deteriorated in BST films less than 24 nm thick. A SEM observation showed the presence of micro-roughness or micro-hillocks in these films, which were confirmed to be caused by Ru oxidation. Therefore, an annealing process of the Ru electrode was added for its planarization, and the CVD process was also improved. As a result, we obtained smooth and finely crystallized ∼ 20-nm-thick BST films with good electrical properties of equivalent SiO2 thickness (teq) ∼ 0.45 nm and leakage current < 1 × 10−7 A/cm2. We also measured properties of BST films deposited on the 3-D Ru electrode. The results are briefly discussed.

scholarly journals Aligned Nanofiber-Guided Bone Regeneration Barrier Incorporated with Equine Bone-Derived Hydroxyapatite for Alveolar Bone Regeneration

Polymers ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 60
Author(s):  
Jae Woon Lim ◽  
Kyoung-Je Jang ◽  
Hyunmok Son ◽  
Sangbae Park ◽  
Jae Eun Kim ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Tissue ◽  
High Speed ◽  
Alveolar Bone ◽  
Guided Bone Regeneration ◽  
Bone Tissue Regeneration ◽  
Calcium Deposition ◽  
Graft Material ◽  
Post Surgery ◽  
Equine Bone

Post-surgery failure of dental implants due to alveolar bone loss is currently critical, disturbing the quality of life of senior dental patients. To overcome this problem, bioceramic or bone graft material is loaded into the defect. However, connective tissue invasion instead of osteogenic tissue limits bone tissue regeneration. The guided bone regeneration concept was adapted to solve this problem and still has room for improvements, such as biochemical similarity or oriented structure. In this article, an aligned electrospun-guided bone regeneration barrier with xenograft equine bone-derived nano hydroxyapatite (EBNH-RB) was fabricated by electrospinning EBNH/PCL solution on high-speed rotating drum collector and fiber characterization, viability and differentiation enhancing properties of mesenchymal dental pulp stem cell on the barrier was determined. EBNH-RB showed biochemical and structural similarity to natural bone tissue electron microscopy image analysis and x-ray diffractometer analysis, and had a significantly better effect in promoting osteogenesis based on the increased bioceramic content by promoting cell viability, calcium deposition and osteogenic marker expression, suggesting that they can be successfully applied to regenerate alveolar bone as a guided bone regeneration barrier.

scholarly journals Strontium Isotope Investigation of Human Mobility Based on Teeth from Cave T1

2021 ◽  
Author(s):  
Ian Moffat ◽  
Renaud Joannes-Boyau ◽  
Les Kinsley ◽  
Malte Willmes ◽  
Rainer Grün
Keyword(s):  
Mass Spectrometry ◽  
Laser Ablation ◽  
Inductively Coupled Plasma ◽  
Human Mobility ◽  
Strontium Isotope ◽  
Human Teeth ◽  
Inductively Coupled ◽  
The People ◽  
Plasma Mass Spectrometry ◽  
Geological Units

Seven adult human teeth from Cave T1 were analysed for trace element concentrations using Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICPMS) and strontium isotope compositions using Laser Ablation Multi-Collector Inductively Coupled Plasma Mass Spectrometry (LA-MC-ICPMS). The aim of this study was to examine whether the people buried in Cave T1 were of local or foreign origin, given the known connection of Philistine Tell es-Safi/ Gath with the Aegean, Cyprus and Anatolia (e.g., Maeir 2012). The results for the burial cave specimens were compared to bioavailable 87Sr/86Sr values obtained from soil and rock samples from key geological units throughout Israel. This comparison shows that at least four of the individuals have 87Sr/86Sr values that correspond to the area immediately surrounding the site. The remaining samples analysed in this study have 87Sr/86Sr values that do not correspond to any mapped samples from Israel. This result is explained not by mobility, but as an artifact of the very low strontium concentration in these teeth, which precludes an effective correction for isobaric interferences during laser ablation analysis.

scholarly journals Cerium-modified cryptomelane: an antibacterial activity against Pseudomonas aeruginosa

2021 ◽  
Vol 947 (1) ◽  
pp. 012027
Author(s):  
Tuyet-Mai Tran-Thuy ◽  
Ngoc-Thuy Nguyen-Thi ◽  
Trong-Phu Tran ◽  
Ngoc-Hung Tran-Le ◽  
Minh-An Dang ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Inductively Coupled Plasma ◽  
Inhibition Zone ◽  
Molar Ratio ◽  
Diffusion Method ◽  
X Ray Diffraction ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

Abstract K-OMS-2 (non-modified sample) and Ce-modified OMS-2 with different Ce-loading amounts were prepared by the refluxed method. The cryptomelane structure and elemental composition of synthesized samples were characterized with X-ray diffraction (XRD) and inductively coupled plasma mass spectrometry (ICP-MS), respectively. ICP-MS analysis revealed ~0.06 – 0.11 of K/Mn molar ratio and the Ce-loading amounts increased from 0 to —11.27 wt.% under increasing Ce-precursor concentration. Antibacterial activity against Pseudomonas aeruginosa was evaluated by the agar well diffusion method. The antibacterial ability against Pseudomonas aeruginosa was not recorded over K-OMS-2 (Ce0; 4.5 wt.% of K; 0 wt.% of Ce) and Ce3 (0.89 wt.% of K; 11.27 wt.% of Ce) samples while Ce2 sample (1.89 wt.% of K; 8.7 wt.% of Ce) showed a comparable antibacterial activity against Pseudomonas aeruginosa with ~12 mm of inhibition-zone diameter. This suggested the potentiality of using metal-modified cryptomelane in acceleration of antimicrobial ability.

Sign in / Sign up

Export Citation Format

Share Document