scholarly journals Formulation and Evaluation of Microwave-Modified Chitosan-Curcumin Nanoparticles—A Promising Nanomaterials Platform for Skin Tissue Regeneration Applications Following Burn Wounds

Polymers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 2608
Author(s):  
Hafiz Muhammad Basit ◽  
Mohd Cairul Iqbal Mohd Amin ◽  
Shiow-Fern Ng ◽  
Haliza Katas ◽  
Shefaat Ullah Shah ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Cell Migration ◽  
Drug Release ◽  
Physicochemical Properties ◽  
Diffusion Mechanism ◽  
Dermal Fibroblasts ◽  
Low Molecular Weight ◽  
Curcumin Nanoparticles ◽  
Modified Chitosan ◽  
Low Molecular Weight Chitosan

Improved physicochemical properties of chitosan-curcumin nanoparticulate carriers using microwave technology for skin burn wound application are reported. The microwave modified low molecular weight chitosan variant was used for nanoparticle formulation by ionic gelation method nanoparticles analyzed for their physicochemical properties. The antimicrobial activity against Staphylococcus aureus and Pseudomonas aeruginosa cultures, cytotoxicity and cell migration using human dermal fibroblasts—an adult cell line—were studied. The microwave modified chitosan variant had significantly reduced molecular weight, increased degree of deacetylation and decreased specific viscosity. The nanoparticles were nano-sized with high positive charge and good dispersibility with entrapment efficiency and drug content in between 99% and 100%, demonstrating almost no drug loss. Drug release was found to be sustained following Fickian the diffusion mechanism for drug release with higher cumulative drug release observed for formulation (F)2. The microwave treatment does not render a destructive effect on the chitosan molecule with the drug embedded in the core of nanoparticles. The optimized formulation precluded selected bacterial strain colonization, exerted no cytotoxic effect, and promoted cell migration within 24 h post application in comparison to blank and/or control application. Microwave modified low molecular weight chitosan-curcumin nanoparticles hold potential in delivery of curcumin into the skin to effectively treat skin manifestations.

scholarly journals Effect of Polysaccharide Sources on the Physicochemical Properties of Bromelain–Chitosan Nanoparticles

Polymers ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1681 ◽  
Author(s):  
Janaína Artem Ataide ◽  
Eloah Favero Gérios ◽  
Letícia Caramori Cefali ◽  
Ana Rita Fernandes ◽  
Maria do Céu Teixeira ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Physicochemical Properties ◽  
Enzyme Stability ◽  
Proteolytic Enzymes ◽  
Chitosan Nanoparticles ◽  
Low Molecular Weight ◽  
Chitosan Oligosaccharide ◽  
Nanoparticle Suspension ◽  
Accelerated Stability ◽  
Low Molecular Weight Chitosan

Bromelain, a set of proteolytic enzymes potential pharmaceutical applications, was encapsulated in chitosan nanoparticles to enhance enzyme stability, and the effect of different chitosan sources was evaluated. Chitosan types (i.e., low molecular weight chitosan, chitosan oligosaccharide lactate, and chitosan from shrimp shells) produced nanoparticles with different physicochemical properties, however in all cases, particle size and zeta potential decreased, and polydispersity index increased after bromelain addition. Bromelain encapsulation was higher than 84% and 79% for protein content and enzymatic activity, respectively, with low molecular weight chitosan presenting the highest encapsulation efficiency. Nanoparticle suspension was also tested for accelerated stability and rheological behavior. For the chitosan–bromelain nanoparticles, an instability index below 0.3 was recorded and, in general, the loading of bromelain in chitosan nanoparticles decreased the cohesiveness of the final suspension.

Production of low molecular weight chitosan by acid and oxidative pathways: Effect on physicochemical properties

2019 ◽  
Vol 123 ◽  
pp. 88-94 ◽  
Author(s):  
Bruna Silva de Farias ◽  
Dara Djenifer Rodrigues Grundmann ◽  
Francisca Zuchoski Rizzi ◽  
Natália Soares Santos Martins ◽  
Tito Roberto Sant'Anna Cadaval Junior ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Physicochemical Properties ◽  
Low Molecular Weight ◽  
Low Molecular Weight Chitosan

Fabrication of cefixime nanoparticles loaded films and their ex vivo antimicrobial effect on periodontitis patient’s saliva

2021 ◽  
Vol 10 ◽  
Author(s):  
Rakeshkumar Parmar ◽  
Mohammad Salman M ◽  
Payal Chauhan
Keyword(s):  
Molecular Weight ◽  
Drug Release ◽  
Ex Vivo ◽  
Chitosan Film ◽  
Inhibition Zone ◽  
Release Profile ◽  
Low Molecular Weight ◽  
Drug Release Profile ◽  
Low Molecular Weight Chitosan

Aim: This study was designed to prepare and evaluate cefixime-loaded nanoparticles containing low molecular weight chitosan films for the enhanced topical treatment of periodontitis. Methods: To fabricate the enhanced antimicrobial films, a nanoprecipitation method for cefixime nanoparticles followed by a solvent evaporation method for these nanoparticles loaded films were adopted in this study. Nine batches of nanoparticles (NPs) with different concentrations of ethyl cellulose and polyvinyl alcohol were prepared and evaluated. Furthermore, nine batches of optimized NPs loaded films with different concentrations of low molecular weight chitosan and glycerol were fabricated and evaluated. Optimized NPs loaded films were assessed for their antimicrobial activity against the periodontitis patient’s saliva samples. Results: The FT-IR spectroscopy and XRD study revealed that there was no interaction between the drug and all other excipients and the drug remained amorphous form in chitosan film. The SEM study revealed that the prepared NPs were spherical in shape and uniformly distributed in chitosan film. In vitro drug release study revealed the NPs have a sustained release profile up to 8 days and NPs loaded films have up to 11 days. The conventional marketed mouth wash shows a low inhibition zone of 5.70 ± 0.043 mm, whereas NPs loaded film shows a higher inhibition zone of 6.72 ± 0.063 mm against periodontal microorganisms present in the patient’s saliva. The stability study revealed that the optimized NPs loaded film shows no dramatic change in drug release profile and folding endurance after six months. Conclusion: This present study highlights the possible usage of cefixime NPs loaded films in enhanced periodontal treatment.

scholarly journals Formulation and evaluation of antifungal agent in a hydrogel containing nanoparticle of low molecular weight chitosan

2020 ◽  
Vol 11 (1) ◽  
pp. 247-259
Author(s):  
Deekshitha H M ◽  
Namratha S Saraf ◽  
Kulkarni P K ◽  
Akhila A R ◽  
Jayaprakash J S
Keyword(s):  
Molecular Weight ◽  
Antifungal Activity ◽  
Drug Release ◽  
Antifungal Agent ◽  
Fungal Infections ◽  
Chitosan Nanoparticles ◽  
Low Molecular Weight ◽  
Formulation Development ◽  
Efficient Treatment ◽  
Low Molecular Weight Chitosan

The research aimed to formulate and prepare hydrogel of low molecular weight chitosan nanoparticles with the help of antifungal agents such as clotrimazole and Nystatin for the topical application. Using the ionic gelation process, placebo and nanoparticles containing drugs were prepared. By cationic interaction is suitable for pH, and gel sodium tripolyphosphate is used as a crosslinker which cross-links the chitosan particles to form the gel, interaction between drug and polymer was observed during the formulation development. Formulated nanoparticle with chitosan and nystatin and clotrimazole showed the mean diameter 273.7nm, 983nm, and 501nm. Clotrimazole and Nystatin had encapsulation capacity was about 74.6% and 63.0%, respectively. In vitro drug release at pH 4.7 chitosan molecules are examined for the version of clotrimazole and nystatin.it has noticed that both the formulation shows sustained drug release about 12 hours. Using SEM, nanoparticles are directed for surface morphology character; it shows that nanoparticles are smooth and spherical in structure. Chitosan itself has the antimicrobial property; it was carried out for the antifungal activity against the fungus Candida Albicans along with the prepared formulations drugs for the comparative study. And the result showed that clotrimazole had maximum fungal growth inhibition next followed by the nystatin and chitosan is least. This inhibitory effect was due to the parameter such as zeta potential and particle size of chitosan nanoparticles. And it shows the least antifungal activity of nanoparticles, which are formulated from the TMC. Hence CTZ and Chitosan were formulated and applied as a natural antifungal agent into nanoparticle to increase the fungal activity. According to the ICH Q1A (R2) guidelines, stability studies are conducted to prove no degradation of the drug. The prepared formulation of chitosan nanoparticles based hydrogel incorporated with clotrimazole and nystatin are promising and significant properties for the efficient treatment of topical fungal infections

Preparation of low-molecular-weight chitosan by enzymatic hydro-lysis for use in peliculture

2021 ◽  
pp. 31-33
Author(s):  
Элана Игоревна Ковалева ◽  
Марина Алексеевна Фролова ◽  
Алексей Иванович Албулов ◽  
Валерий Петрович Варламов ◽  
Анатолий Константинович Елисеев
Keyword(s):  
Molecular Weight ◽  
Enzymatic Hydrolysis ◽  
Environmental Factors ◽  
Low Molecular Weight ◽  
Technological Parameters ◽  
Low Molecular Weight Chitosan

Хитозан и его производные обладают множеством свойств, которые позволяют применять его в пчеловодстве. Он повышает устойчивость организма пчел к неблагоприятным факторам внешней среды и к возбудителям различных заболеваний. В результате проведенных исследований разработаны технологические параметры получения низкомолекулярного хитозана методом ферментативного гидролиза. Chitosan and its derivatives have many properties that allow them to be used in beekeeping. It increases the resistance of the bee organism to adverse environmental factors and to pathogens of various diseases. As a result of the conducted research, the technological parameters for the production of low-molecular-weight chitosan by enzymatic hydrolysis were developed.

Liposome coated with low molecular weight chitosan and its potential use in ocular drug delivery

2009 ◽  
Vol 379 (1) ◽  
pp. 131-138 ◽  
Author(s):  
Ning Li ◽  
Chunyang Zhuang ◽  
Mi Wang ◽  
Xiyang Sun ◽  
Shufang Nie ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Molecular Weight ◽  
Ocular Drug Delivery ◽  
Low Molecular Weight ◽  
Low Molecular Weight Chitosan ◽  
Potential Use
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