Effect of Acyl Chain Length and Unsaturation on Physicochemical Properties and Transfection Efficiency of N-Acyl-Substituted Low-Molecular-Weight Chitosan

2012 ◽  
Vol 101 (1) ◽  
pp. 268-282 ◽  
Author(s):  
Rhishikesh Mandke ◽  
Jagdish Singh
Keyword(s):  
Molecular Weight ◽  
Physicochemical Properties ◽  
Chain Length ◽  
Transfection Efficiency ◽  
Acyl Chain ◽  
Low Molecular Weight ◽  
Acyl Chain Length ◽  
Low Molecular Weight Chitosan

Thermal Properties of Acylated Low Molecular Weight Chitosans

2019 ◽  
Vol 41 (2) ◽  
pp. 207-207
Author(s):  
Shu Xian Tiew and Misni Misran Shu Xian Tiew and Misni Misran
Keyword(s):  
Molecular Weight ◽  
Hydrogen Bonding ◽  
Chain Length ◽  
Acyl Chain ◽  
Low Molecular Weight ◽  
Scanning Electron Micrographs ◽  
Scanning Calorimetry ◽  
Acyl Chain Length ◽  
Medium Chain Length ◽  
Acyl Chains

Acylated low molecular weight chitosans (LChA) were prepared from nucleophilic acylation of chitosan using acid anhydrides of short and medium chain length (4 - 10) to study the response of applied heat as a function of acyl chain length. Thermogravimetric analysis (TGA) revealed the decomposition of LChA consisted of glucosamine and acyl-glucosamine units around 141 - 151and#176;C to 400 - 410and#176;C. Both TGA and differential scanning calorimetry (DSC) analyses indicated that the introduction of acyl groups disrupted the hydrogen bonding of chitosan, the effect was more prominent as the degree of substitution and chain length of LChA increased. Grafting of acyl chains lowered the kinematic viscosity of LChA as the disruption of hydrogen bonding led to decreased hydrodynamic volume. Field emission scanning electron micrographs showed that LChA with longer chains having larger particle size due to bigger occupancy volume of acyl chains during spray drying.

scholarly journals Effect of Polysaccharide Sources on the Physicochemical Properties of Bromelain–Chitosan Nanoparticles

Polymers ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1681 ◽  
Author(s):  
Janaína Artem Ataide ◽  
Eloah Favero Gérios ◽  
Letícia Caramori Cefali ◽  
Ana Rita Fernandes ◽  
Maria do Céu Teixeira ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Physicochemical Properties ◽  
Enzyme Stability ◽  
Proteolytic Enzymes ◽  
Chitosan Nanoparticles ◽  
Low Molecular Weight ◽  
Chitosan Oligosaccharide ◽  
Nanoparticle Suspension ◽  
Accelerated Stability ◽  
Low Molecular Weight Chitosan

Bromelain, a set of proteolytic enzymes potential pharmaceutical applications, was encapsulated in chitosan nanoparticles to enhance enzyme stability, and the effect of different chitosan sources was evaluated. Chitosan types (i.e., low molecular weight chitosan, chitosan oligosaccharide lactate, and chitosan from shrimp shells) produced nanoparticles with different physicochemical properties, however in all cases, particle size and zeta potential decreased, and polydispersity index increased after bromelain addition. Bromelain encapsulation was higher than 84% and 79% for protein content and enzymatic activity, respectively, with low molecular weight chitosan presenting the highest encapsulation efficiency. Nanoparticle suspension was also tested for accelerated stability and rheological behavior. For the chitosan–bromelain nanoparticles, an instability index below 0.3 was recorded and, in general, the loading of bromelain in chitosan nanoparticles decreased the cohesiveness of the final suspension.

Low molecular weight chitosan–protamine conjugate for siRNA delivery with enhanced stability and transfection efficiency

RSC Advances ◽  
2016 ◽  
Vol 6 (112) ◽  
pp. 110951-110963 ◽  
Author(s):  
Sushilkumar Patil ◽  
Priyanka Bhatt ◽  
Rohan Lalani ◽  
Jitendra Amrutiya ◽  
Imran Vhora ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Transfection Efficiency ◽  
Sirna Delivery ◽  
Low Molecular Weight ◽  
Low Molecular Weight Chitosan ◽  
Low Cytotoxicity ◽  
Enhanced Stability

Chitosan is among the few polymers with high biocompatibility and low cytotoxicity.

Effect of acyl chain length on transfection efficiency and toxicity of polyethylenimine

2009 ◽  
Vol 378 (1-2) ◽  
pp. 201-210 ◽  
Author(s):  
Latha Aravindan ◽  
Katrina A. Bicknell ◽  
Gavin Brooks ◽  
Vitaliy V. Khutoryanskiy ◽  
Adrian C. Williams
Keyword(s):  
Chain Length ◽  
Transfection Efficiency ◽  
Acyl Chain ◽  
Acyl Chain Length

scholarly journals Formulation and Evaluation of Microwave-Modified Chitosan-Curcumin Nanoparticles—A Promising Nanomaterials Platform for Skin Tissue Regeneration Applications Following Burn Wounds

Polymers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 2608
Author(s):  
Hafiz Muhammad Basit ◽  
Mohd Cairul Iqbal Mohd Amin ◽  
Shiow-Fern Ng ◽  
Haliza Katas ◽  
Shefaat Ullah Shah ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Cell Migration ◽  
Drug Release ◽  
Physicochemical Properties ◽  
Diffusion Mechanism ◽  
Dermal Fibroblasts ◽  
Low Molecular Weight ◽  
Curcumin Nanoparticles ◽  
Modified Chitosan ◽  
Low Molecular Weight Chitosan

Improved physicochemical properties of chitosan-curcumin nanoparticulate carriers using microwave technology for skin burn wound application are reported. The microwave modified low molecular weight chitosan variant was used for nanoparticle formulation by ionic gelation method nanoparticles analyzed for their physicochemical properties. The antimicrobial activity against Staphylococcus aureus and Pseudomonas aeruginosa cultures, cytotoxicity and cell migration using human dermal fibroblasts—an adult cell line—were studied. The microwave modified chitosan variant had significantly reduced molecular weight, increased degree of deacetylation and decreased specific viscosity. The nanoparticles were nano-sized with high positive charge and good dispersibility with entrapment efficiency and drug content in between 99% and 100%, demonstrating almost no drug loss. Drug release was found to be sustained following Fickian the diffusion mechanism for drug release with higher cumulative drug release observed for formulation (F)2. The microwave treatment does not render a destructive effect on the chitosan molecule with the drug embedded in the core of nanoparticles. The optimized formulation precluded selected bacterial strain colonization, exerted no cytotoxic effect, and promoted cell migration within 24 h post application in comparison to blank and/or control application. Microwave modified low molecular weight chitosan-curcumin nanoparticles hold potential in delivery of curcumin into the skin to effectively treat skin manifestations.

Production of low molecular weight chitosan by acid and oxidative pathways: Effect on physicochemical properties

2019 ◽  
Vol 123 ◽  
pp. 88-94 ◽  
Author(s):  
Bruna Silva de Farias ◽  
Dara Djenifer Rodrigues Grundmann ◽  
Francisca Zuchoski Rizzi ◽  
Natália Soares Santos Martins ◽  
Tito Roberto Sant'Anna Cadaval Junior ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Physicochemical Properties ◽  
Low Molecular Weight ◽  
Low Molecular Weight Chitosan
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