scholarly journals Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 736
Author(s):  
Juliana Helena da Silva Barros ◽  
André Luiz Rodrigues Roque ◽  
Samanta Cristina das Chagas Xavier ◽  
Kátia Cristina Silva Nascimento ◽  
Helena Keiko Toma ◽  
...  
Keyword(s):  
Dna Analysis ◽  
Feeding Habits ◽  
Random Amplified Polymorphic Dna ◽  
Enzyme Electrophoresis ◽  
Multilocus Enzyme Electrophoresis ◽  
Dehydrogenase Gene ◽  
Mitochondrial Cytochrome B ◽  
Mitochondrial Cytochrome B Gene ◽  
High Bootstrap ◽  
Trypanosoma Dionisii

This study describes the morphological, biochemical, and molecular differences among Trypanosoma dionisii isolates from hemocultures of hematophagous (Desmodus rotundus; n = 2) and insectivorous (Lonchorhina aurita; n = 1) bats from the Atlantic Rainforest of Rio de Janeiro, Brazil. Fusiform epimastigotes from the hematophagous isolates were elongated, whereas those of the insectivorous isolate were stumpy, reflected in statistically evident differences in the cell body and flagellum lengths. In the hemocultures, a higher percentage of trypomastigote forms (60%) was observed in the hematophagous bat isolates than that in the isolate from the insectivorous bat (4%), which demonstrated globular morphology. Three molecular DNA regions were analyzed: V7V8 (18S rDNA), glycosomal glyceraldehyde 3-phosphate dehydrogenase gene, and mitochondrial cytochrome b gene. The samples were also subjected to multilocus enzyme electrophoresis and random amplified polymorphic DNA analysis. All isolates were identified as T. dionisii by phylogenetic analysis. These sequences were clustered into two separate subgroups with high bootstrap values according to the feeding habits of the bats from which the parasites were isolated. However, other T. dionisii samples from bats with different feeding habits were found in the same branch. These results support the separation of the three isolates into two subgroups, demonstrating that different subpopulations of T. dionisii circulate among bats.

scholarly journals Differential Gene Expression in Benznidazole-Resistant Trypanosoma cruzi Parasites

2005 ◽  
Vol 49 (7) ◽  
pp. 2701-2709 ◽  
Author(s):  
Diana Villarreal ◽  
Philippe Nirdé ◽  
Mallorie Hide ◽  
Christian Barnabé ◽  
Michel Tibayrenc
Keyword(s):  
Gene Expression ◽  
Trypanosoma Cruzi ◽  
Differential Gene Expression ◽  
Drug Exposure ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Enzyme Electrophoresis ◽  
Wild Type ◽  
Resistance Level ◽  
Differential Gene

ABSTRACT We analyzed the differential gene expression among representative Trypanosoma cruzi stocks in relation to benznidazole exposures using a random differentially expressed sequences (RADES) technique. Studies were carried out with drug pressure both at the natural susceptibility level of the wild-type parasite (50% inhibitory concentration for the wild type) and at different resistance levels. The pattern of differential gene expression performed with resistant stocks was compared to the population structure of this parasite, established by random amplified polymorphic DNA analysis and multilocus enzyme electrophoresis. A RADES band polymorphism was observed, and over- or underexpression was linked to the resistance level of the stock. The analysis of RADES bands suggested that different products may be involved in benznidazole resistance mechanisms. No significant association was found between phylogenetic clustering and benznidazole susceptibility. Benznidazole resistance may involve several mechanisms, depending on the level of drug exposure.

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