Pulsed-Field Gel Electrophoresis Is More Discriminating Than Multilocus Enzyme Electrophoresis and Random Amplified Polymorphic DNA Analysis for Typing Pyogenic Streptococci

1997 ◽  
Vol 34 (4) ◽  
pp. 226-229 ◽  
Author(s):  
Frédéric Bert ◽  
Catherine Branger ◽  
Nicole Lambert-Zechovsky
Keyword(s):  
Gel Electrophoresis ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Pulsed Field Gel Electrophoresis ◽  
Enzyme Electrophoresis ◽  
Pulsed Field ◽  
Multilocus Enzyme Electrophoresis

scholarly journals Genetic characterization ofMycobacterium aviumisolates recovered from humans and animals in Australia

1996 ◽  
Vol 116 (1) ◽  
pp. 41-49 ◽  
Author(s):  
M. M. Feizabadi ◽  
I. D. Robertson ◽  
D. V. Cousins ◽  
D. Dawson ◽  
W. Chew ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mycobacterium Avium ◽  
Gel Electrophoresis ◽  
Genetic Characterization ◽  
Genetic Relationships ◽  
Pulsed Field Gel Electrophoresis ◽  
Pfge Type ◽  
Enzyme Electrophoresis ◽  
Pulsed Field ◽  
Multilocus Enzyme Electrophoresis

SummaryGenetic relationships amongst 115 mainly Australian isolates ofMycobacterium aviumwere assessed using multilocus enzyme electrophoresis (MEE). The isolates were divided into 58 electrophoretic types (ETs), with a mean genetic diversity of 0·29. Isolates from humans were closely related to but distinct from those cultured from birds, whilst some porcine isolates belonged to the same ETs as certain human isolates. Pulsed field gel electrophoresis (PFGE) was used to differentiate related isolates, and those from birds and some from other animals, including pigs, were distinguished from the human isolates. The results of MEE and PFGE suggested that certain strains ofM. aviummay be transmitted between birds and pigs, but there was no clear evidence of transmission to humans. The serovar of theM. aviumisolates was not obviously related to their ET assignment or their PFGE type.

Disparity between Multilocus Enzyme Electrophoresis, Microsatellite Markers and Pulsed-Field Gel Electrophoresis in epidemiological tracking of Candida albicans

2010 ◽  
Vol 82 (3) ◽  
pp. 265-281 ◽  
Author(s):  
Marcelo Fabiano Gomes Boriollo ◽  
Ricardo Antunes Dias ◽  
João Evangelista Fiorini ◽  
Nelma de Mello Silva Oliveira ◽  
Denise Madalena Palomari Spolidório ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Microsatellite Markers ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Enzyme Electrophoresis ◽  
Pulsed Field ◽  
Multilocus Enzyme Electrophoresis

scholarly journals Pulsed-Field Gel Electrophoresis Is More Efficient than Ribotyping and Random Amplified Polymorphic DNA Analysis in Discrimination of Pasteurella haemolytica Strains

1999 ◽  
Vol 37 (2) ◽  
pp. 380-385 ◽  
Author(s):  
Angeli Kodjo ◽  
Laurence Villard ◽  
Chantal Bizet ◽  
Jean-Louis Martel ◽  
Richard Sanchis ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Dna Analysis ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Geographic Origin ◽  
Pulsed Field Gel Electrophoresis ◽  
Pasteurella Haemolytica ◽  
Pulsed Field ◽  
Rapd Pattern ◽  
Almost All

One hundred thirty-three strains of Pasteurella haemolytica of both biotypes (90 and 43 strains of biotypes A and T, respectively) and almost all the serotypes were subjected to ribotyping, random amplified polymorphic DNA (RAPD) analysis, and pulsed-field gel electrophoresis (PFGE) analysis for epidemiological purposes. A total of 15 patterns recorded as ribotypes HA to HO were found for the P. haemolytica biotype A strains, with ribotypes HA, HC, and HD being encountered most often (66 strains [74%]); and 20 ribotypes, designated HA′ to HT′, that were clearly distinct from those observed for biotype A strains were observed for strains of biotype T. RAPD analysis generated a total of 44 (designated Rp1 to Rp44) and 15 (designated Rp1′ to Rp 15′) unique RAPD patterns for biogroup A and biogroup T, respectively. Analysis of the data indicated that a given combined ribotype-RAPD pattern could be observed for biotype A strains of different serotypes, whatever the zoological or geographic origin, whereas this was not the case for biotype T strains. PFGE appeared to be more efficient in strain discrimination since selected strains from various zoological or geographical origins harboring the same ribotype-RAPD group were further separated into unique entities.

scholarly journals Staphylococcus aureus strains of type 95. Spread of a single clone

1994 ◽  
Vol 113 (3) ◽  
pp. 463-470 ◽  
Author(s):  
V. T. Rosdahl ◽  
W. Witte ◽  
M. Musser ◽  
J. O. Jarløv
Keyword(s):  
Staphylococcus Aureus ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Enzyme Electrophoresis ◽  
Restriction Fragments ◽  
Pulsed Field ◽  
Multilocus Enzyme Electrophoresis ◽  
Single Clone ◽  
Clonal Origin ◽  
Reference Strains

SUMMARYStaphylococcus aureus strains of type 95 in Denmark have increased to a frequency of 20% of the total S. aureus population. A clonal origin and possible subdivision of these strains have been discussed. In the present investigation 35 epidemiologically unrelated S. aureus strains of type 95 as well as reference strains of other types have been analysed by other typing techniques including lectintyping, multilocus enzyme electrophoresis and pulsed-field gel electrophoresis of genomic restriction fragments. No subdivision could be achieved based on any of these methods and a clonal origin seems therefore possible.

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