scholarly journals An Ultra-Sensitive Technique: Using Pv-mtCOX1 qPCR to Detect Early Recurrences of Plasmodium vivax in Patients in the Brazilian Amazon

Pathogens ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 19
Author(s):  
Laila R. A. Barbosa ◽  
Emanuelle L. da Silva ◽  
Anne C. G. de Almeida ◽  
Yanka E. A. R. Salazar ◽  
André M. Siqueira ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Plasmodium Vivax ◽  
Blood Smear ◽  
Detection Method ◽  
Brazilian Amazon ◽  
Early Recurrence ◽  
True Incidence ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Smear Testing

Background: Early recurrence of Plasmodium vivax is a challenge for malaria control in the field, particularly because this species is associated with lower parasitemia, which hinders diagnosis and monitoring through blood smear testing. Early recurrences, defined as the persistence of parasites in the peripheral blood despite adequate drug dosages, may arise from resistance to chloroquine. The objective of the study was to estimate early recurrence of P. vivax in the Brazilian Amazon by using a highly-sensitive detection method, in this case, PCR. Methods: An ultra-sensitive qPCR that targeted mitochondrial DNA was used to compare a standard qPCR that targeted 18S rDNA to detect early recurrence of P. vivax in very low densities in samples from patients treated with chloroquine. Results: Out of a total of 312 cases, 29 samples (9.3%) were characterized as recurrences, from which 3.2% (10/312) were only detected through ultra-sensitive qPCR testing. Conclusions: Studies that report the detection of P. vivax early recurrences using light microscopy may severely underestimate their true incidence.

Highly sensitive detection method of retinoblastoma genetic predisposition and biomarkers

2021 ◽  
Author(s):  
Jessica Le Gall ◽  
Catherine Dehainault ◽  
Camille Benoist ◽  
Alexandre Matet ◽  
Livia Lumbroso-Le Rouic ◽  
...  
Keyword(s):  
Genetic Predisposition ◽  
Detection Method ◽  
Sensitive Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

SERS-based immunoassay of anti-cyclic citrullinated peptide for early diagnosis of rheumatoid arthritis

RSC Advances ◽  
2014 ◽  
Vol 4 (62) ◽  
pp. 32924-32927 ◽  
Author(s):  
Hyangah Chon ◽  
Sangyeop Lee ◽  
Rui Wang ◽  
So-Young Bang ◽  
Hye-Soon Lee ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Early Diagnosis ◽  
Detection Method ◽  
Sensitive Detection ◽  
Clinical Tool ◽  
Cyclic Citrullinated Peptide ◽  
Immunoassay Technique ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Citrullinated Peptide

We report a highly sensitive detection method for anti-CCP autoantibodies using a SERS-based magnetic immunosensor. The proposed immunoassay technique is expected to be a new clinical tool for the early diagnosis of rheumatoid arthritis (RA).

scholarly journals Highly Sensitive Detection of 4-Methylimidazole Using a Terahertz Metamaterial

Sensors ◽  
2018 ◽  
Vol 18 (12) ◽  
pp. 4304 ◽  
Author(s):  
Hee Shin ◽  
Hae Jang ◽  
Gyeongsik Ok
Keyword(s):  
Resonance Frequency ◽  
Detection Method ◽  
Sensitive Detection ◽  
Fdtd Simulation ◽  
Resonator Structure ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Difference Time ◽  
Insight Into ◽  
Good Agreement

In this study, we demonstrated a highly sensitive detection method of 4-methylimidazole (4-MeI), a carcinogenic material, by using a terahertz (THz) metamaterial at a THz region. The THz metamaterials were fabricated with a metal array, using an electric-field-coupled inductor-capacitor (ELC) resonator structure, and a finite-difference time-domain (FDTD) simulation showed good agreement with the experimental results. We measured the THz spectra of the metamaterials to detect the 4-MeI concentrations of 0, 1, 2, 5, 10, 15, and 20 mg/L. The resonance frequency of the metamaterial was shifted by, approximately, 8 GHz and transmittance at the resonance frequency increased to 2 × 10−3, as the concentration was increased, up to 20 mg/L. Our study provides new insight into the application of metamaterials in detecting carcinogens, using a THz technique.

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