SERS-based immunoassay of anti-cyclic citrullinated peptide for early diagnosis of rheumatoid arthritis

RSC Advances ◽  
2014 ◽  
Vol 4 (62) ◽  
pp. 32924-32927 ◽  
Author(s):  
Hyangah Chon ◽  
Sangyeop Lee ◽  
Rui Wang ◽  
So-Young Bang ◽  
Hye-Soon Lee ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Early Diagnosis ◽  
Detection Method ◽  
Sensitive Detection ◽  
Clinical Tool ◽  
Cyclic Citrullinated Peptide ◽  
Immunoassay Technique ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Citrullinated Peptide

We report a highly sensitive detection method for anti-CCP autoantibodies using a SERS-based magnetic immunosensor. The proposed immunoassay technique is expected to be a new clinical tool for the early diagnosis of rheumatoid arthritis (RA).

Highly sensitive detection method of retinoblastoma genetic predisposition and biomarkers

2021 ◽  
Author(s):  
Jessica Le Gall ◽  
Catherine Dehainault ◽  
Camille Benoist ◽  
Alexandre Matet ◽  
Livia Lumbroso-Le Rouic ◽  
...  
Keyword(s):  
Genetic Predisposition ◽  
Detection Method ◽  
Sensitive Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Efficacy of three ELISA measurements of anti-cyclic citrullinated peptide antibodies in the early diagnosis of rheumatoid arthritis

2005 ◽  
Vol 43 (11) ◽  
Author(s):  
Antonio Fernández-Suárez ◽  
Sonsoles Reneses ◽  
Ingeborg Wichmann ◽  
Rita Criado ◽  
Antonio Núñez
Keyword(s):  
Rheumatoid Arthritis ◽  
Early Diagnosis ◽  
High Specificity ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Cyclic Citrullinated Peptide ◽  
Citrullinated Peptide ◽  
Peptide Antibodies

AbstractThe objective of the present study was to determine the efficacy of anti-cyclic citrullinated peptide (anti-CCP) antibody detection in the early diagnosis of rheumatoid arthritis (RA), as well as to compare three commercially available enzyme-linked immunosorbent assay (ELISA) kits used to detect such antibodies. We analysed the presence of anti-CCP antibodies in the sera of 78 patients who had been newly referred from primary healthcare centres to the Early Polyarthritis Unit. We also included in the study a group of 50 healthy controls. None of the patients had previously received treatment for the disease. After 1-year follow-up, the diagnosis of RA was confirmed in 53 of these patients. The ELISA kits under study were IMMUNOSCAN RA (Euro-Diagnostica AB), QUANTA Lite™ CCP IgG ELISA (INOVA Diagnostic) and DIA-STAT™ Anti-CCP (Axis-Shield Diagnostics); the sensitivity obtained was 52.8%, 58.5% and 52.8%, respectively, with 100% specificity for all three kits. Anti-CCP antibodies detected the presence of RA in 26% of patients without positive rheumatoid factor (RF). The sum of anti-CCP antibodies or the presence of RF gave a sensitivity of up to 67%, with specificity ranging between 94 and 97%. Anti-CCP antibodies show high specificity for the diagnosis of RA. The three ELISAs analysed offer the same degree of diagnostic accuracy.

Highly sensitive detection of the human papillomavirus E6 protein by DNA-protected silver nanoclusters and the intrinsic mechanism

2019 ◽  
Vol 43 (37) ◽  
pp. 14944-14951
Author(s):  
Xiang-Yu Jia ◽  
Ya-Rong Xue ◽  
Chun-Xia Zhang ◽  
Quan Luo ◽  
Yuqing Wu
Keyword(s):  
Human Papillomavirus ◽  
Early Diagnosis ◽  
Silver Nanoclusters ◽  
Sensitive Detection ◽  
Biological Application ◽  
Intrinsic Mechanism ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
E6 Protein

The present study not only supplies a good approach for the early diagnosis of HPV-related cancer but also enriches the biological application of AgNCs–dsDNA.

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