scholarly journals The Linoleic Acid: Dihomo-γ-Linolenic Acid Ratio (LA:DGLA)—An Emerging Biomarker of Zn Status

Nutrients ◽  
2017 ◽  
Vol 9 (8) ◽  
pp. 825 ◽  
Author(s):  
Marija Knez ◽  
James Stangoulis ◽  
Maria Glibetic ◽  
Elad Tako
Keyword(s):  
Linoleic Acid ◽  
Linolenic Acid ◽  
Acid Ratio ◽  
Zn Status

scholarly journals Updates on the Linoleic Acid: Dihomo-γ-Linolenic Acid Ratio (LA: DGLA)- as an Emerging Biomarker of Zinc Status (P24-019-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Elad Tako
Keyword(s):  
Linoleic Acid ◽  
Linolenic Acid ◽  
Serum Zinc ◽  
Significant Negative Correlation ◽  
Mean Value ◽  
World Health ◽  
Zn Deficiency ◽  
Acid Ratio ◽  
Zn Status

Abstract Objectives Zinc (Zn) deficiency is affecting 17% of the world's population. Although whole blood, plasma, and urine Zn decrease in severe Zn deficiency, accurate assessment of Zn status is difficult. Hence, as suggested by the World Health Organization, sensitive and specific biomarkers of Zn status are needed. Previously, we suggested the concept of the essential role of Zn for ∆6-desaturase activity. For the first time, we tested and implemented a previously unexplored biomarker of Zn status related to erythrocyte ∆6-desaturation, the LA: DGLA (linoleic acid: dihomo-γ-linolenic acid) ratio. We evaluated the sensitivity of the erythrocyte LA: DGLA ratio to changes in supplemental Zn intake. A significant negative correlation was found between dietary Zn deficiency and the LA: DGLA ratio. Our objective is to provide further and recent evidence to demonstrate that the LA: DGLA ratio is a useful and effective biomarker to assess Zn status. Methods The efficacy of the LA: DGLA ratio to predict the Zn status of subjects consuming a wheat-based diet, a representative diet of the target Zn-deficient populations, was evaluated in vivo (Gallus gallus). Two groups of birds (n = 15) were fed two different diets, a “high-Zn” diet (46.5 ppm Zn) and a “low-Zn” diet (32.8 ppm Zn), for 6 weeks. Dietary Zn intake, body weight, serum zinc, and the erythrocyte fatty acid profile were assessed. Results Serum and tissues (feather and nail) Zn concentrations were greater in the high-Zn group (P < 0.05). Duodenal expression of Zn transporters demonstrated a higher mean value in the tissues collected from the birds fed a low-Zn diet (n = 15, P < 0.05). Hepatic ∆6-desaturase expression showed a higher mean value in birds fed high-Zn diets (P < 0.05). LA: DGLA ratio was higher (P < 0.05) in the low-Zn group of birds at all-time points. Even though both groups of birds were fed Zn-deficient diets, with only 14 ppm differential in dietary Zn content, still the LA: DGLA ratio differentiated clearly between the groups, which demonstrates the sensitivity of the biomarker to change in accordance with dietary Zn intake. Conclusions Recent evidence demonstrate the potential of the LA: DGLA ratio to be used as an additional biomarker of Zn status in humans. To date, research shows that the LA: DGLA ratio corresponds to dietary Zn manipulations, both in animals and humans. Funding Sources USDA-ARS. Supporting Tables, Images and/or Graphs

scholarly journals Factors affecting the polyunsaturated fatty acid content of the plasma lipids of sheep

1968 ◽  
Vol 22 (4) ◽  
pp. 681-688 ◽  
Author(s):  
J. H. Moore ◽  
R. C. Noble ◽  
W. Steele
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Stearic Acid ◽  
Linolenic Acid ◽  
Plasma Lipids ◽  
Fatty Acid Content ◽  
Cholesteryl Esters ◽  
Blood Samples ◽  
Plasma Triglycerides ◽  
Acid Ratio

1. In Expt 1, four adult wether sheep were given diets of hay or dried grass in an alternating sequence of feeding treatments. Each treatment period lasted for 20 days and blood samples were taken from the sheep on the last day of each period. When the diet of hay (linoleic: linolenic acid ratio 1.40) was replaced by one of dried grass (linoleic: linolenic acid ratio 0.28), there was an increase in the concentration of linolenic acid and a decrease in the concentration of linoleic acid in the plasma cholesteryl esters and phospholipids. There was an increase in the concentration of stearic acid and a decrease in the concentration of palmitic acid in the plasma triglycerides. Dietary change did not affect the composition of the plasma unesterified fatty acids.2. In Expt 2, two adult wether sheep, each with a rumen fistula, were given daily intraruminal infusions of 60 g of ‘linolenic’ or ‘linoleic’ acids (both about 70% pure) over a period of 5 days. The infusion treatments were then reversed. Blood samples were taken at the end of each infusion period. Intraruminal infusions of ‘linolenic’ acid increased the concentration of linolenic acid in the plasma cholesteryl esters and phospholipids and increased the concentration of stearic acid in the plasma triglycerides. The infusions of ‘linoleic’ acid increased the concentration of linoleic acid in the plasma cholesteryl esters and phospholipids. No appreciable changes were observed in the composition of the plasma unesterified fatty acids.

102 EFFECTS OF LINOLEIC ACID/LINOLENIC ACID RATIO ON OOCYTE MATURATION AND FERTILIZATION IN PREPUBERTAL SHEEP

2016 ◽  
Vol 28 (2) ◽  
pp. 181
Author(s):  
V. Ghaffarilaleh ◽  
R. Ghaffarilaleh ◽  
G. Ghaffarilaleh
Keyword(s):  
Linoleic Acid ◽  
Linolenic Acid ◽  
Embryo Development ◽  
Oocyte Maturation ◽  
Fertilization Rate ◽  
Alpha Linolenic Acid ◽  
Cleavage Rate ◽  
Acid Ratio ◽  
Maturation Rate ◽  
Epidermal Growth

The aim of this study was to clarify the effects of linoleic acid (LA)/alpha-linolenic acid (ALA) ratio on oocyte maturation and fertilization. Prepubertal sheep ovaries were sliced, then cumulus-oocyte complexes were matured in a media based on TCM-199 supplemented with FSH, LH, epidermal growth factor, BSA fraction V, cysteamine, glutamine, and sodium pyruvate for 24 h in different ratios of LA/ALA (0, 1 : 1, 2 : 1, 4 : 1, and 10 : 1). After maturation, 169 oocytes stained for maturation rate. In the second experiment, 176 presumptive zygotes from 227 fertilized oocytes were stained for fertilization rate. In the third experiment, 570 oocytes were used for embryo development. The generalized linear model was used for analysis. The results showed that LA/ALA at the ratios of 1 : 1, 2 : 1, and 4 : 1 significantly (P < 0.05) improved the MII percentages and the 10 : 1 ratio was detrimental. Fertilization rate was not significant between treatments, but the two pro-nuclei percentage was significantly increased for the ratios of 1 : 1, 2 : 1, and 4 : 1 (P < 0.05). Cleavage rate for the ratio of 2 : 1 and 4 : 1 was significantly higher (P < 0.05). The ratio of 10 : 1 was detrimental for embryo development, and none of the cleaved embryos reached the blastocyst rate. In conclusion, the ratio of 10 : 1 LA/ALA was detrimental and resulted in degenerated oocytes and subsequently for prepubertal sheep embryos, whereas lower ratios of LA/ALA were beneficial for oocyte competition and fertilisation.

Effects of dietary linolenic acid to linoleic acid ratio on growth performance, proximate composition and fatty acid contents of pacu ( Piaractus mesopotamicus )

2021 ◽  
Author(s):  
Ligia Uribe Gonçalves ◽  
Carlos Andre Amaringo Cortegano ◽  
Rafael Simões Coelho Barone ◽  
Evandro Kleber Lorenz ◽  
José Eurico Possebon Cyrino
Keyword(s):  
Fatty Acid ◽  
Linoleic Acid ◽  
Growth Performance ◽  
Linolenic Acid ◽  
Proximate Composition ◽  
Piaractus Mesopotamicus ◽  
Acid Ratio ◽  
Dietary Linolenic Acid

scholarly journals The Linoleic Acid: Dihomo-γ-Linolenic Acid Ratio (LA: DGLA)- an Emerging Biomarker of Zinc Status

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1842-1842
Author(s):  
Elad Tako
Keyword(s):  
Linolenic Acid ◽  
Serum Zinc ◽  
Mean Value ◽  
Intestinal Microbiome ◽  
Funding Sources ◽  
Acid Ratio ◽  
Zn Status ◽  
And Function ◽  
Related Proteins

Abstract Objectives Assessment of erythrocyte linoleic: dihomo-γ-linolenic acid (LA: DGLA) ratio as a biomarker of Zn status, and structure and function of cecal microbiota following the consumption of a Zn-biofortified wheat diet. Methods The efficacy of the LA: DGLA ratio to predict the Zn status of subjects consuming a wheat-based diet, a representative diet of the target Zn-deficient populations, was evaluated in-vivo (Gallus gallus). Two groups (n = 15) were fed two different diets, a “high-Zn” diet (46.5 ppm Zn) and a “low-Zn” diet (32.8 ppm Zn), for 6 weeks. Dietary Zn-intake, body-weight, serum zinc, intestinal microbiome, expression of zinc related proteins, and the erythrocyte fatty acid profile were assessed (LA: DGLA ratio). Results Serum and tissues Zn concentrations were greater in the high-Zn group (P &lt; 0.05). Duodenal expression of Zn transporters demonstrated a higher mean value in the tissues collected from the birds fed a low-Zn diet (n = 15, P &lt; 0.05). Hepatic ∆6-desaturase expression showed a higher mean value in birds fed high-Zn diets (P &lt; 0.05). LA: DGLA ratio was higher (P &lt; 0.05) in the low-Zn group of birds at all-time points. Even though both groups of birds were fed Zn-deficient diets, with only 14 ppm differential in dietary Zn content, still the LA: DGLA ratio differentiated clearly between the groups, which demonstrates the sensitivity of the biomarker to change in accordance with dietary Zn intake. Conclusions Recent evidence demonstrate the potential of the LA: DGLA ratio to be used as an additional biomarker of Zn status in humans. To date, research shows that the LA: DGLA ratio corresponds to dietary Zn manipulations, both in animal model and humans. Funding Sources N/A.

Effects of the dietary linoleic acid to linolenic acid ratio for Nile tilapia (Oreochromis niloticus) breeding females

Aquaculture ◽  
2020 ◽  
Vol 516 ◽  
pp. 734625
Author(s):  
Tamira Maria Orlando ◽  
Táfanie Valácio Fontes ◽  
Renan Rosa Paulino ◽  
Luis David Solis Murgas ◽  
Jose Fernando López-Olmeda ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Linolenic Acid ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Dietary Linoleic Acid ◽  
Acid Ratio ◽  
Nile Tilapia Oreochromis Niloticus
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