102 EFFECTS OF LINOLEIC ACID/LINOLENIC ACID RATIO ON OOCYTE MATURATION AND FERTILIZATION IN PREPUBERTAL SHEEP

2016 ◽  
Vol 28 (2) ◽  
pp. 181
Author(s):  
V. Ghaffarilaleh ◽  
R. Ghaffarilaleh ◽  
G. Ghaffarilaleh
Keyword(s):  
Linoleic Acid ◽  
Linolenic Acid ◽  
Embryo Development ◽  
Oocyte Maturation ◽  
Fertilization Rate ◽  
Alpha Linolenic Acid ◽  
Cleavage Rate ◽  
Acid Ratio ◽  
Maturation Rate ◽  
Epidermal Growth

The aim of this study was to clarify the effects of linoleic acid (LA)/alpha-linolenic acid (ALA) ratio on oocyte maturation and fertilization. Prepubertal sheep ovaries were sliced, then cumulus-oocyte complexes were matured in a media based on TCM-199 supplemented with FSH, LH, epidermal growth factor, BSA fraction V, cysteamine, glutamine, and sodium pyruvate for 24 h in different ratios of LA/ALA (0, 1 : 1, 2 : 1, 4 : 1, and 10 : 1). After maturation, 169 oocytes stained for maturation rate. In the second experiment, 176 presumptive zygotes from 227 fertilized oocytes were stained for fertilization rate. In the third experiment, 570 oocytes were used for embryo development. The generalized linear model was used for analysis. The results showed that LA/ALA at the ratios of 1 : 1, 2 : 1, and 4 : 1 significantly (P < 0.05) improved the MII percentages and the 10 : 1 ratio was detrimental. Fertilization rate was not significant between treatments, but the two pro-nuclei percentage was significantly increased for the ratios of 1 : 1, 2 : 1, and 4 : 1 (P < 0.05). Cleavage rate for the ratio of 2 : 1 and 4 : 1 was significantly higher (P < 0.05). The ratio of 10 : 1 was detrimental for embryo development, and none of the cleaved embryos reached the blastocyst rate. In conclusion, the ratio of 10 : 1 LA/ALA was detrimental and resulted in degenerated oocytes and subsequently for prepubertal sheep embryos, whereas lower ratios of LA/ALA were beneficial for oocyte competition and fertilisation.

scholarly journals Effects of the Oocyte Maturation Rate on Clinical Outcomes of Conventional IVF and ICSI in Female Patients Aged ≥38 Years

2020 ◽  
Author(s):  
shuang liu ◽  
Hongjun Yu ◽  
Baoshan Li ◽  
Chunyi Li ◽  
Dongkai Cheng
Keyword(s):  
Pregnancy Rate ◽  
Oocyte Maturation ◽  
Birth Rate ◽  
Formation Rate ◽  
Live Birth Rate ◽  
Fertilization Rate ◽  
Blastocyst Formation ◽  
Oocyte Maturity ◽  
Cleavage Rate ◽  
Maturation Rate

Abstract Research Question: The average female reproductive potential peaks at age 25 and then begins to decline. As women are delaying childbearing, the prevalence of infertility has risen, leading to increasing demand for assisted reproductive technology (ART). Oocyte quality remains the most important issue during in vitro fertilization (IVF) cycles. This study investigated the effects of the oocyte maturation rate (OMR) on clinical outcomes of conventional IVF and intracytoplasmic sperm injection (ICSI) in female patients aged ≥38 years. Design: A retrospective analysis of 6562 infertile patients who were treated with IVF/ICSI at the reproductive medicine center of our hospital from January 2011 to December 2017 was performed. According to the oocyte maturity (the ratio of the number of mature oocytes to the number of oocytes) on the day of egg collection, the patients were divided into three groups: group A (oocyte maturity ≤30%, n=422), and group B (oocyte maturity from 30-75%, n=1290), and group C (oocyte maturity ≥75%, n=4850). The patient age, years of infertility years, days of gonadotropin (Gn), Gn dosage, serum luteinizing hormone (LH), estradiol (E2), and progesterone (P) levels on the day of human chorionic Gn (HCG) injection, E2 levels per mature oocyte, E2 levels per oocyte, number of mature oocytes, oocyte recovery rate, fertilization rate, cleavage rate, excellent embryo rate, blastocyst formation rate, pregnancy rate, and live birth rate were compared among the three groups. Results: Factors including age, years of infertility, number of eggs obtained, number of mature eggs, normal fertilization rate, cleavage rate, excellent embryo rate, blastocyst formation rate, pregnancy rate, and live birth rate were all found to be related to oocyte maturity on the day of HCG administration (P<0.05). Conclusions: There is a close relationship between oocyte maturity and embryo quality, and a low OMR may be related to a poor ovarian response or decreased sensitivity to Gn. Therefore, a low OMR may affect the fertilization and embryonic development potential in elderly patients undergoing IVF/ICSI, thus affecting the pregnancy rate.

Cysteamine and b-mercaptoethanol supplementation improves the in vitro embryo development in buffaloes (Bubalus bubalis)

2016 ◽  
Author(s):  
Vijay Singh ◽  
A. K. Misra ◽  
Suresh Kumar ◽  
Champak Barman
Keyword(s):  
Embryo Development ◽  
Oocyte Maturation ◽  
In Vitro Maturation ◽  
Cumulus Cells ◽  
Bubalus Bubalis ◽  
Cleavage Rate ◽  
Maturation Rate ◽  
Morula Stage ◽  
The Media

The objective of the present experiment was to investigate the effect of cysteamine and b-mercaptoethanol supplementation on in -vitro maturation, cleavage of oocytes and development of embryo in buffalo (Bubalus bubalis). Oocytes were aspirated from abattoir ovarian follicles of 3-10 mm diameter followed by maturation in the media in vitro containing cysteamine/b-mercaptoethanol (treatment) and without antioxidant (control). Matured oocytes were co-incubated with sperm (approx.1×106/ml) of Murrah bull in mSOF medium using heparin (10 μg/ml). After 22 h of oocyte-sperm incubation, fertilized oocytes were stripped of cumulus cells and cultured in mSOF medium for 8 days to study embryo development. The oocyte maturation rate improved significantly (P<0.05) following addition of 50 or 100 μM of cysteamine and 10, 50 and 100 μM of b- mercaptoethanol (ME), respectively as compared to control. The cleavage rate was found to be significantly (P<0.05) higher at 50 and 100 μM of cysteamine and at all concentrations of b-mercaptoethanol as compared to control and development of embryos to morula stage was significantly (P<0.05) improved with 50 μM cysteamine/ b-mercaptoethanol.

scholarly journals Effect of Alpha-Linolenic Acid on Oocyte Maturation and Embryo Development in Pigs

2017 ◽  
Vol 21 (2) ◽  
pp. 205-213 ◽  
Author(s):  
Ji-Eun Lee ◽  
Hwangbo Yong ◽  
Hwa-Young Kim ◽  
Won-Hee Lee ◽  
Hee-Tae Cheong ◽  
...  
Keyword(s):  
Linolenic Acid ◽  
Embryo Development ◽  
Oocyte Maturation ◽  
Alpha Linolenic Acid

Higher Oxidative Stability of Alpha-linolenic Acid Than Linoleic Acid in Nanoemulsions: a Comparison Between Bulk Flaxseed Oil and its O/W Nanoemulsions

2021 ◽  
Author(s):  
Masoomeh Zeinalzadegan ◽  
Maryam Nejadmansouri ◽  
Mohammad-Taghi Golmakani ◽  
Gholam Reza Mesbahi ◽  
David Julian McClements ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Oxidative Stability ◽  
Linolenic Acid ◽  
Flaxseed Oil ◽  
Alpha Linolenic Acid

Cleavage Rate of in vitro Matured Oocytes Fertilized with Conventional Semen in Buffaloes

2021 ◽  
Author(s):  
M.H. Pitroda ◽  
K.P. Khillare ◽  
M.B. Amle ◽  
M.D. Meshram ◽  
A.B. Mali ◽  
...  
Keyword(s):  
Fertilization Rate ◽  
Cleavage Rate ◽  
Embryo Production ◽  
Quick Recovery ◽  
Slaughter House ◽  
Maturation Rate ◽  
Aspiration Technique ◽  
Current Scenario ◽  
In Vitro Embryo Production

Background: In vitro embryo production in buffaloes has gained much importance in this current scenario due to ever increasing population and high demand of milk and meat. Slaughter house derived bubaline ovaries are a cheap and abundant source of cumulus oocyte complexes.Methods: Oocytes from the buffalo ovarian follicles were recovered by aspiration technique as it facilitates quick recovery. Total 155 ovaries were used in the present study. Surface follicles were measured using vernier calliper and categorized into three groups viz. less than 3 mm, 3-5 mm and greater than 5 mm based on follicular diameter and oocytes were processed for IVM, IVF and IVC using conventional non sorted semen.Result: Overall percentage of small, medium and large follicles in the ovaries were recorded as 16.29 ± 0.94%, 8.14±0.60%, 5.35 ± 0.76%, respectively. Overall recovery rate of COCs was 38%. The percentage of these oocytes were 16.74% (A), 15.25% (B), 25.26% (C), 18.33% (D) and 29.87% (E) respectively. Maturation rate of oocytes were 81.96 ± 2.70%. Fertilization rate was 74.98 ± 3.87%, Cleavage rate % was 40.84±2.51% and Blastocyst percentage was 21.57±1.75% respectively. Application of in vitro embryo production technique using slaughter house ovaries can salvage the genetic potential of bubaline species.

scholarly journals Combined signature of rumen microbiome and metabolome in dairy cows with different feed intake levels

2020 ◽  
Vol 98 (3) ◽  
Author(s):  
Yeqing Q Li ◽  
Yumeng M Xi ◽  
Zedong D Wang ◽  
Hanfang F Zeng ◽  
Zhaoyu Han
Keyword(s):  
Linoleic Acid ◽  
Dairy Cows ◽  
Linolenic Acid ◽  
Relative Abundance ◽  
Feed Intake ◽  
Metabolic Pathways ◽  
Acid Metabolism ◽  
Ruminal Fermentation ◽  
Alpha Linolenic Acid ◽  
Rumen Microbiome

Abstract Feed intake is a major factor in maintaining the balance between ruminal fermentation and the microbial community of dairy cows. To explore the relationship among feed intake, microbial metabolism, and ruminal fermentation, we examined the combined signatures of the microbiome and metabolome in dairy cows with different feed intake levels. Eighteen dairy cows were allocated to high feed intake (HFI), medium feed intake (MFI), and low feed intake (LFI) groups according to their average daily feed intake. 16S rDNA sequencing results revealed that the relative abundance of Firmicutes in the HFI group was significantly higher than that in the MFI and LFI groups (P &lt; 0.05). The ratio of Bacteroidetes to Firmicutes was significantly lower in the HFI group than in the MFI and LFI groups (P &lt; 0.05). The relative abundance of Lachnospiraceae_unclassified, Veillonellaceae_unclassified, and Saccharofermentants was significantly higher in the HFI group than in the LFI and MFI groups (P &lt; 0.05). The relative abundance of Erysipelotrichaceae_unclassified and Butyrivibrio was significantly higher in the HFI group than in the MFI and LFI groups (P &lt; 0.05). Ultra high performance liquid chromatography-mass spectrometry revealed five key pathways, including the linoleic acid metabolism pathway, alpha-linolenic acid metabolism, arginine and proline metabolism, glutathione metabolism, and valine, leucine, and isoleucine biosynthesis, which are closely related to energy and amino acid metabolism. Linoleic acid, glutamate, alpha-linolenic acid, l-methionine, and l-valine levels were significantly lower in the HFI group than in the MFI and LFI groups (q &lt; 0.05), while the relative content of glutamate was significantly lower in the MFI group than in the LFI group (q &lt; 0.05). Stearic acid content was significantly higher in the HFI group than in the LFI group (q &lt; 0.05). Our findings provide insight into the rumen microbiome of dairy cows with different feed intake and the metabolic pathways closely associated with feed intake in early-lactating cows. The candidates involved in these metabolic pathways may be useful for identifying variations in feed intake. The signatures of the rumen microbiome and metabolome in dairy cows may help make decisions regarding feeding.

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