Optimal dietary alpha-linolenic acid/linoleic acid ratio improved digestive and absorptive capacities and target of rapamycin gene expression of juvenile grass carp (Ctenopharyngodon idellus)

2015 ◽  
Vol 22 (6) ◽  
pp. 1251-1266 ◽  
Author(s):  
Y.-Y. Zeng ◽  
W.-D. Jiang ◽  
Y. Liu ◽  
P. Wu ◽  
J. Zhao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Linoleic Acid ◽  
Linolenic Acid ◽  
Grass Carp ◽  
Target Of Rapamycin ◽  
Ctenopharyngodon Idellus ◽  
Alpha Linolenic Acid ◽  
Acid Ratio ◽  
Absorptive Capacities

102 EFFECTS OF LINOLEIC ACID/LINOLENIC ACID RATIO ON OOCYTE MATURATION AND FERTILIZATION IN PREPUBERTAL SHEEP

2016 ◽  
Vol 28 (2) ◽  
pp. 181
Author(s):  
V. Ghaffarilaleh ◽  
R. Ghaffarilaleh ◽  
G. Ghaffarilaleh
Keyword(s):  
Linoleic Acid ◽  
Linolenic Acid ◽  
Embryo Development ◽  
Oocyte Maturation ◽  
Fertilization Rate ◽  
Alpha Linolenic Acid ◽  
Cleavage Rate ◽  
Acid Ratio ◽  
Maturation Rate ◽  
Epidermal Growth

The aim of this study was to clarify the effects of linoleic acid (LA)/alpha-linolenic acid (ALA) ratio on oocyte maturation and fertilization. Prepubertal sheep ovaries were sliced, then cumulus-oocyte complexes were matured in a media based on TCM-199 supplemented with FSH, LH, epidermal growth factor, BSA fraction V, cysteamine, glutamine, and sodium pyruvate for 24 h in different ratios of LA/ALA (0, 1 : 1, 2 : 1, 4 : 1, and 10 : 1). After maturation, 169 oocytes stained for maturation rate. In the second experiment, 176 presumptive zygotes from 227 fertilized oocytes were stained for fertilization rate. In the third experiment, 570 oocytes were used for embryo development. The generalized linear model was used for analysis. The results showed that LA/ALA at the ratios of 1 : 1, 2 : 1, and 4 : 1 significantly (P < 0.05) improved the MII percentages and the 10 : 1 ratio was detrimental. Fertilization rate was not significant between treatments, but the two pro-nuclei percentage was significantly increased for the ratios of 1 : 1, 2 : 1, and 4 : 1 (P < 0.05). Cleavage rate for the ratio of 2 : 1 and 4 : 1 was significantly higher (P < 0.05). The ratio of 10 : 1 was detrimental for embryo development, and none of the cleaved embryos reached the blastocyst rate. In conclusion, the ratio of 10 : 1 LA/ALA was detrimental and resulted in degenerated oocytes and subsequently for prepubertal sheep embryos, whereas lower ratios of LA/ALA were beneficial for oocyte competition and fertilisation.

scholarly journals Alpha-linolenic acid and linoleic acid differentially regulate the skeletal muscle secretome of obese Zucker rats

2018 ◽  
Vol 50 (8) ◽  
pp. 580-589
Author(s):  
Alex Rajna ◽  
Heather Gibling ◽  
Ousseynou Sarr ◽  
Sarthak Matravadia ◽  
Graham P. Holloway ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Linoleic Acid ◽  
Linolenic Acid ◽  
Glucose Homeostasis ◽  
Secreted Proteins ◽  
Whole Body ◽  
Zucker Rats ◽  
Alpha Linolenic Acid ◽  
Obese Zucker Rats

Evidence shows that proteins secreted from skeletal muscle influence a broad range of metabolic signaling pathways. We previously reported that essential polyunsaturated fatty acids (PUFA) improved whole-body glucose homeostasis in obese Zucker rats; however, the mechanisms underlying these benefits remain enigmatic. While PUFA and obesity influence skeletal muscle function, their effects on the secretome are unknown. The aim of this work was to determine if improvements in whole-body glucose homeostasis in obese Zucker rats fed diets supplemented with either linoleic acid (LA) or alpha-linolenic acid (ALA) for 12 wk are related to changes in the skeletal muscle secretome. Secreted proteins were identified with a predictive bioinformatic analysis of microarray gene expression from red tibialis anterior skeletal muscle. Approximately 130 genes were differentially expressed (false discovery rate = 0.05) in obese rats compared with lean controls. The expression of 15 genes encoding secreted proteins was differentially regulated in obese controls, obese LA-supplemented, and obese ALA-supplemented rats compared with lean controls. Five secreted proteins ( Col3a1, Col15a1, Pdgfd, Lyz2, and Angptl4) were differentially regulated by LA and ALA. Most notably, ALA supplementation reduced Angptl4 gene expression compared with obese control and obese-LA supplemented rats and reduced circulating ANGPTL4 serum concentrations. ALA also influenced Angptl4 gene expression and ANGPTL4 secretion from differentiated rat L6 myotubes. Altogether, the present data indicate that obesity has a greater global impact on skeletal muscle gene expression than either essential PUFA; however, LA and ALA may exert their metabolic benefits in part by regulating the skeletal muscle secretome.

Higher Oxidative Stability of Alpha-linolenic Acid Than Linoleic Acid in Nanoemulsions: a Comparison Between Bulk Flaxseed Oil and its O/W Nanoemulsions

2021 ◽  
Author(s):  
Masoomeh Zeinalzadegan ◽  
Maryam Nejadmansouri ◽  
Mohammad-Taghi Golmakani ◽  
Gholam Reza Mesbahi ◽  
David Julian McClements ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Oxidative Stability ◽  
Linolenic Acid ◽  
Flaxseed Oil ◽  
Alpha Linolenic Acid
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