scholarly journals Gastroprotective Effect of Enteral Nutrition Formula in Mice Injected Subcutaneously with Indomethacin

Nutrients ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 3297
Author(s):  
Yoshiaki Yamagishi ◽  
Rei Saiki ◽  
Takeshi Yoshimi ◽  
Toshiyuki Kudo ◽  
Kiyomi Ito
Keyword(s):  
Gastrointestinal Tract ◽  
Enteral Nutrition ◽  
Oral Administration ◽  
Protective Effect ◽  
Direct Interaction ◽  
Gastric Lesions ◽  
Icr Mice ◽  
Gastroprotective Effect ◽  
Time Courses

We have previously shown that two enteral nutrition formulas suppressed gastric lesions induced by the oral administration of indomethacin (IND) in mice. However, the mechanism of their protective effect is unknown. In this study, the effect of the two enteral nutrition formulas on gastric lesions induced by subcutaneous IND injection was investigated, with the objective of exploring the possibility that they may interact directly with IND in the gastrointestinal tract. Ten-week-old, male, ICR mice were fasted, then orally given either purified water, Mermed® One, or 2-fold diluted Terumeal® 2.0α as enteral nutrition formula (25 mL/kg). IND was injected subcutaneously at 20 mg/kg after 30 min, and the stomach was removed 6 h later and fixed in formalin. The number and area of lesions in the stomachs of mice given enteral nutrition formula was reduced to 56–89% and 34–61%, respectively, compared with the mice given purified water. The time courses of plasma IND concentrations were comparable among the three groups. These results suggested that the effect of these enteral nutrition formulas on gastric lesions did not originate from their direct interaction with IND in the gastrointestinal tract or their effect on the disposition of IND.

scholarly journals Gastroprotective effect of ethylacetate fraction of the leaves of Hannoa klaineana on aspirin and histamine-induced gastric ulcer in rats

2020 ◽  
Vol 8 (1) ◽  
pp. 70
Author(s):  
Ibrahim Abubakar ◽  
Hassan Muhammad Yankuzo ◽  
Yusha'u Shuaibu Baraya ◽  
Mu'azu Abubakar Gusau
Keyword(s):  
Protective Effect ◽  
Ph Value ◽  
Gastrointestinal Diseases ◽  
Gastric Volume ◽  
Total Acidity ◽  
Gastric Lesions ◽  
Ulcer Index ◽  
Ulcer Disease ◽  
Gastroprotective Effect ◽  
Ulcer Model

Background: Peptic ulcer disease remains endemic in our society affecting about four million people every year worldwide. Hannoa klaineana is used traditionally in the treatment of various gastrointestinal diseases including ulcer.Aim: This study aims at evaluating the gastroprotective effect of ethylacetate fraction of the leaves of Hannoa klaineana (Simaroubaceae).Methods: The gastroprotective effect of ethylacetate fraction of the Hannoa klaineana (50, 100 and 200mg/kg b.wt) was evaluated using aspirin and histamine induced ulcer models.Results: In aspirin-induced ulcer model, the ethylacetate fraction of the Hannoa klaineana demonstrated significant (p<0.001) decreased in mean ulcer index with the maximum protective effect (99.84%) at 200 mg/kg against the gastric damages. While histamine-induced ulcer model, the solvent fraction significantly (p<0.001) decreased mean ulcer index with the protective effect up to 99.83% against the gastric lesions. In both models, a significant (p<0.001) increased in pH value coupled with significant (p<0.001) decreased in gastric volume, free and total acidity in rats pre-treated with varying doses of the ethylacetate fraction was found.Conclusion: The mechanism of gastroprotective effects of ethylacetate fraction of the Hannoa klaineana could be attributed to its ability to stimulate prostaglandins secretion or possess prostaglandins like-substances or suppression of histamine-induced vasospastic effect and gastric secretion.   

Protective effect of trillin against ethanol-induced acute gastric lesions in an animal model

RSC Advances ◽  
2016 ◽  
Vol 6 (24) ◽  
pp. 20081-20088 ◽  
Author(s):  
Tong Chen ◽  
Wenjiao Jiang ◽  
Huixin Zhang ◽  
Xintong You ◽  
Manling Liu ◽  
...  
Keyword(s):  
Animal Model ◽  
Protective Effect ◽  
Gastric Lesions ◽  
Gastroprotective Effect ◽  
Mucosal Lesions

The purpose of the present study was to evaluate gastroprotective effect of trillin on mucosal lesions induced by ethanol.

Gastric protective effect of peripheral PYY through PYY preferring receptors in anesthetized rats

2002 ◽  
Vol 283 (5) ◽  
pp. G1035-G1041 ◽  
Author(s):  
Keishi Kawakubo ◽  
Hong Yang ◽  
Yvette Taché
Keyword(s):  
Protective Effect ◽  
Receptor Agonist ◽  
Peptide Yy ◽  
Gastric Injury ◽  
Blood Levels ◽  
Gastric Lesions ◽  
Gastroprotective Effect ◽  
Anesthetized Rats ◽  
Peak Blood

The influence of intravenous peptide YY (PYY) on the gastric injury induced by 45% ethanol was investigated in urethane-anesthetized rats. PYY (25, 75, 125, and 250 pmol · kg−1 · h−1) significantly reduced gastric lesions by 36, 59, 40, and 38%, respectively. Antibody against ratPYY (2 mg/rat) injected intravenously completely prevented the gastroprotective effect of intravenous PYY (75 pmol · kg−1 · h−1), whereas injected intracisternally (460 μg/20 μl), it significantly prevented intracisternal PYY (24 pmol/rat)-induced 58% reduction of ethanol lesions but not that induced by intravenous PYY. Vagotomy did not influence the gastroprotective effect of intravenous PYY. The Y1/“PYY-preferring” receptor agonist [Pro34]PYY (75 pmol · kg−1 ·h−1iv) significantly decreased ethanol-induced gastric lesions by 82%, whereas [Leu31, Pro34]NPY, a Y1/Y3 agonist, and PYY-(3–36), a Y2 agonist, had no effect. These data indicate that PYY-infused intravenously at doses reported to mimic postprandial peak blood levels prevents ethanol-induced gastric injury through vagal independent pathways and PYY-preferring receptors.

scholarly journals Study of the Inhibitory Effects of Enteral Nutrition Formula on Indomethacin-Induced Gastric Lesions in Mice

Nutrients ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 3058
Author(s):  
Takeshi Yoshimi ◽  
Yoshiaki Yamagishi ◽  
Issei Kanegawa ◽  
Megumi Suda ◽  
Rei Saiki ◽  
...  
Keyword(s):  
Enteral Nutrition ◽  
Treated Group ◽  
Gastric Ulcers ◽  
Gastric Lesions ◽  
Inhibitory Effects ◽  
Significant Difference ◽  
Time Courses ◽  
Dose Dependent Decrease ◽  
Dose Dependent ◽  
Comparable Time

We investigated the effects of enteral nutrition formula on non-steroidal anti-inflammatory drug (NSAID)-induced gastric lesions in mice. Male ICR mice aged 7–9 weeks old were fasted, then orally given either purified water, Mermed® One, or 2-fold diluted Terumeal® 2.0α as enteral nutrition (25 or 50 mL/kg each). Indomethacin (IND) was orally administered at 20 mg/kg after 30 min, and the stomach was removed 6 h later and fixed in formalin. The number and area of lesions in the stomachs of the mice given enteral nutrition showed a significant, dose-dependent decrease compared to the purified water-treated group, and no significant difference was seen between the two enteral nutrition-treated groups. Comparable time courses of plasma IND concentrations suggest that enteral nutrition does not inhibit gastrointestinal absorption of IND. Our findings indicate that administering enteral nutrition could inhibit the onset of NSAID-induced gastric ulcers.

Oral administration of MSG increases expression of glutamate receptors and transporters in the gastrointestinal tract of young piglets

Amino Acids ◽  
2013 ◽  
Vol 45 (5) ◽  
pp. 1169-1177 ◽  
Author(s):  
Jun Zhang ◽  
Yulong Yin ◽  
Xu Gang Shu ◽  
Tiejun Li ◽  
Fengna Li ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Oral Administration ◽  
Glutamate Receptors

scholarly journals Actions of crude hydroalcoholic extract of Pfaffia sp on gastrointestinal tract

2003 ◽  
Vol 46 (3) ◽  
pp. 355-360 ◽  
Author(s):  
Cristina Setim Freitas ◽  
Maria Fernanda Rodrigues de Paula ◽  
Lia Rieck ◽  
Maria Consuelo Andrade Marques
Keyword(s):  
Gastrointestinal Tract ◽  
Acid Secretion ◽  
Restraint Stress ◽  
Wistar Rats ◽  
Protective Action ◽  
Folk Medicine ◽  
Gastric Lesions ◽  
Hydroalcoholic Extract ◽  
Female Wistar Rats ◽  
Duodenal Lumen

The plants that compound the Pfaffia genus are used in folk medicine to treat gastric disturbances. This study examined the effects of a crude hydroalcoholic extract of Pfaffia sp on the gastrointestinal tract. Female Wistar rats were pretreated orally (p.o.) with the hydroalcoholic extract of Pfaffia (0.5, 1 and 2 g.kg-1) before the induction of ulcer with hypothermic restraint stress (HRS), ethanol (ET) or indomethacin (IND). Control animals received water (C) or ranitidine (60mg/kg) p.o. The hydroalcoholic extract of Pfaffia (0.5, 1 and 2 mg.kg-1) protected rats against HRS and ET - induced ulcers, but was not able to protect the gastric mucosa against IND - induced ulcers. When injected into the duodenal lumen, the hydroalcoholic extract of Pfaffia inhibited basal and stimulated acid secretion in pylorus-ligated rats. These results indicate that this plant has a protective action against gastric lesions of the mucosa involving the reduction of gastric acid secretion.

scholarly journals Estimation of permeability to bacteriophages of intestinal mucosa of chickens with eimeriosis

2019 ◽  
Vol 49 (2) ◽  
pp. 57-63
Author(s):  
N. V. Davydova ◽  
V. Yu. Koptev ◽  
Yu. N. Kozlova ◽  
L. I. Sulimova ◽  
V. N. Afonyushkin ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Oral Administration ◽  
Protective Effect ◽  
Intestinal Mucosa ◽  
Clinical Picture ◽  
Biological Material ◽  
Probiotic Strain ◽  
Field Isolates ◽  
Different Types ◽  
Inflammatory Processes

In the course of the study permeability of intestinal mucosa of chickens suffering from eimeriosis while treating them with various veterinary drugs, including probiotics, was evaluated. The simulation of a typical clinical picture of eimeriosis was carried out by oral administration of suspension with coccidial oocysts (1.6 × 105/head) using a probe. To create different forms and different intensity of inflammatory processes, chickens that received various anticoccidial preparations and probiotic strain Bacillus subtilis were infected with eimeria. According to the data from an autopsy, it was found that the use of a spore probiotic based on Bacillus subtilis and anticoccidial drugs containing robenidine hydrochloride and salinomycin had a positive protective effect when treating chickens from eimeriosis. A similar picture was observed when assessing permeability of intestinal mucosa as affected by bacteriophage, whereby permeability decreased with the use of probiotics and the above-mentioned active agents. In general, the decrease in productivity was significant in all groups. However, the effect of spore-based probiotics was quite pronounced against the background of eimeria polyresistance. In the situation where anticoccidial drugs are less effective, the use of a spore-based probiotic can have a noticeable protective effect. The effect of all anticoccidial drugs under study on the concentration of oocysts and the state of the mucosa was insignificant, which indicated polyresistance of different types of eimeria isolated from biological material to these drugs. The analysis of the intestinal mucosa integrity, based on the study of mucosa permeability to bacteriophages and a Johnson and Reid scoring procedure showed that a spore probiotic based on B. subtilis and anticoccidial drugs containing robenidine and salinomycin had the best protective effect against eimeriosis caused by field isolates of eimeria. When treating chickens suffering from eimeriosis caused by polyresistant forms of E. acervulina and E. tenella, it is advisable to use probiotics alongside with drugs based on robenidine and salinomycin.

scholarly journals Beneficial effects of mycophenolate mofetil on cardiotoxicity induced by tacrolimus in wistar rats

2016 ◽  
Vol 242 (4) ◽  
pp. 448-455 ◽  
Author(s):  
Hanen Ferjani ◽  
Rim Timoumi ◽  
Ines Amara ◽  
Salwa Abid ◽  
Abedellatif Achour ◽  
...  
Keyword(s):  
Dna Damage ◽  
Mycophenolate Mofetil ◽  
Oral Administration ◽  
Protective Effect ◽  
Cardiac Toxicity ◽  
De Novo ◽  
Antioxidant Activities ◽  
Solid Organ Transplantation ◽  
Protein Carbonyl ◽  
Solid Organ

The immunosuppressive drug tacrolimus (TAC) is used clinically to reduce the rejection rate in transplant patients. TAC has contributed to an increased prevalence of cardiovascular disease in patients receiving solid organ transplantation. Mycophenolate mofetil (MMF), a potent inhibitor of de novo purine synthesis, is known to prevent ongoing rejection in combination with TAC. In the present study, we investigated the antioxidant and antigenotoxic effect of MMF on TAC-induced cardiotoxicity in rats. Oral administration of TAC at 2.4, 24, and 60 mg/kg b.w. corresponding, respectively, to 1, 10, and 25% of LD50 for 24 h caused cardiac toxicity in a dose-dependant manner. TAC increased significantly DNA damage level in hearts of treated rats. Furthermore, it increased malondialdehyde (MDA) and protein carbonyl (PC) levels and decreased catalase (CAT) and superoxide dismutase (SOD) activities. The oral administration of MMF at 50 mg/kg b.w. simultaneously with TAC at 60 mg/kg b.w. proved a significant cardiac protection by decreasing DNA damage, MDA, and PC levels, and by increasing the antioxidant activities of CAT and SOD. Thus, our study showed, for the first time, the protective effect of MMF against cardiac toxicity induced by TAC. This protective effect was mediated via an antioxidant process.

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