scholarly journals Oral Administration of Sargassum horneri Improves the HDM/DNCB-Induced Atopic Dermatitis in NC/Nga Mice

Nutrients ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2482 ◽  
Author(s):  
Eui Jeong Han ◽  
Ilekuttige Priyan Shanura Fernando ◽  
Hyun-Soo Kim ◽  
You-Jin Jeon ◽  
Dissanayaka Mudiyanselage Dinesh Madusanka ◽  
...  
Keyword(s):  
Immune Response ◽  
Atopic Dermatitis ◽  
Oral Administration ◽  
Ethanol Extract ◽  
Transepidermal Water Loss ◽  
Sargassum Horneri ◽  
Protective Effects ◽  
Expression Levels ◽  
Abnormal Immune Response ◽  
Ifn Γ

The present study investigated the protective effects of Sargassum horneri (S. horneri) ethanol extract (SHE) against atopic dermatitis (AD), known as an abnormal immune response in house dust mite (HDM)/2,4-dinitrochlorobenzene (DNCB)-stimulated NC/Nga mice. The oral administration of SHE attenuated the AD symptoms, including the skin dermatitis severity, transepidermal water loss (TEWL), and ear edema in HDM/DNCB-stimulated mice. Moreover, the histological analysis revealed that SHE improved epidermal hyperplasia and hyperkeratosis, and reduced the dermal infiltrations of mast cells and eosinophils. Moreover, SHE downregulated the expression levels of cytokines (interleukin (IL)-6, IL-10, and interferon (IFN)-γ) and chemokines (Regulated on Activation, Normal T Cell Expressed and Secreted (RANTES), Eotaxin, and Thymus and activation-regulated chemokine (TARC)) by decreasing the expression levels of atopic initiators (IL-25 and IL-33) in HDM/DNCB-stimulated skin. The oral administration of SHE decreased the spleen size, reducing expression levels of AD-related cytokines (IL-4, IL-5, IL-6, IL-10, IL-13, IFN-γ, and TARC) by regulating the expressions of Tbx21 (T-bet), GATA Binding Protein 3 (GATA-3), and Signal transducer and activator of transcription 3 (STAT3). Moreover, SHE significantly attenuated the serum immunoglobulin (Ig)G1 and IgG2a levels in HDM/DNCB-stimulated mice. Collectively, these results suggest that S. horneri could be an ingredient of functional food against abnormal immune response.

scholarly journals Gomisin M2 Ameliorates Atopic Dermatitis-like Skin Lesions via Inhibition of STAT1 and NF-κB Activation in 2,4-Dinitrochlorobenzene/Dermatophagoides farinae Extract-Induced BALB/c Mice

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4409
Author(s):  
Jinjoo Kang ◽  
Soyoung Lee ◽  
Namkyung Kim ◽  
Hima Dhakal ◽  
Taeg-Kyu Kwon ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Oral Administration ◽  
Skin Diseases ◽  
Nuclear Translocation ◽  
Skin Lesions ◽  
Biologically Active ◽  
Therapeutic Effects ◽  
Dermatophagoides Farinae ◽  
Tnf Α ◽  
Ifn Γ

The extracts of Schisandra chinensis (Turcz.) Baill. (Schisandraceae) have various therapeutic effects, including inflammation and allergy. In this study, gomisin M2 (GM2) was isolated from S. chinensis and its beneficial effects were assessed against atopic dermatitis (AD). We evaluated the therapeutic effects of GM2 on 2,4-dinitrochlorobenzene (DNCB) and Dermatophagoides farinae extract (DFE)-induced AD-like skin lesions with BALB/c mice ears and within the tumor necrosis factor (TNF)-α and interferon (IFN)-γ-stimulated keratinocytes. The oral administration of GM2 resulted in reduced epidermal and dermal thickness, infiltration of tissue eosinophils, mast cells, and helper T cells in AD-like lesions. GM2 suppressed the expression of IL-1β, IL-4, IL-5, IL-6, IL-12a, and TSLP in ear tissue and the expression of IFN-γ, IL-4, and IL-17A in auricular lymph nodes. GM2 also inhibited STAT1 and NF-κB phosphorylation in DNCB/DFE-induced AD-like lesions. The oral administration of GM2 reduced levels of IgE (DFE-specific and total) and IgG2a in the mice sera, as well as protein levels of IL-4, IL-6, and TSLP in ear tissues. In TNF-α/IFN-γ-stimulated keratinocytes, GM2 significantly inhibited IL-1β, IL-6, CXCL8, and CCL22 through the suppression of STAT1 phosphorylation and the nuclear translocation of NF-κB. Taken together, these results indicate that GM2 is a biologically active compound that exhibits inhibitory effects on skin inflammation and suggests that GM2 might serve as a remedy in inflammatory skin diseases, specifically on AD.

scholarly journals Combretum quadrangulare Extract Attenuates Atopic Dermatitis-Like Skin Lesions through Modulation of MAPK Signaling in BALB/c Mice

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 2003 ◽  
Author(s):  
Ju-Hyoung Park ◽  
Min Hee Hwang ◽  
Young-Rak Cho ◽  
Seong Su Hong ◽  
Jae-Shin Kang ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Immunoglobulin E ◽  
Ethanol Extract ◽  
Skin Lesions ◽  
Transepidermal Water Loss ◽  
Mapk Signaling ◽  
Chronic Inflammatory Disease ◽  
Mitogen Activated Protein Kinase ◽  
Mrna Levels ◽  
Cytokines And Chemokines

Atopic dermatitis (AD) is a chronic inflammatory disease. Combretum quadrangulare (C. quadrangulare) is used as a traditional medicine to improve various pathologies in Southeast Asia. In this study, we investigated the effects of C. quadrangulare ethanol extract (CQ) on 1-chloro-2,4-dinitrobenzene (DNCB)-induced AD like skin lesions in BALB/c mice. After administration with CQ (100, 200, and 400 mg/kg) for 6 weeks, AD symptoms, protein expression, immunoglobulin E (IgE), thymus and activation-regulated chemokine (TARC), and ceramidase level were measured in skin lesions of DNCB-induced BALB/c mice. CQ group improved the dermatitis score, skin pH, transepidermal water loss (TEWL), and skin hydration. Furthermore, histological analysis revealed that CQ attenuated the increased epidermal thickness and infiltration of mast cells caused by DNCB. CQ also increased the expression of filaggrin, and reduced the expression of ceramidase, serum IgE level, and the number of eosinophils. CQ effectively inhibited cytokines and chemokines such as interleukin (IL)-6, IL-13, TARC, and thymic stromal lymphopoietin (TSLP) at the mRNA levels, as well as the activation of mitogen-activated protein kinase (MAPK), including extracellular signal-regulated kinase (ERK), c-jun N-terminal kinase (JNK), and p38 in the skin lesions. Taken together, these findings demonstrate that CQ may be an effective treatment of AD-like skin lesions by inhibiting the expression of inflammatory mediators via the MAPK signaling pathways.

Sargassum horneri ethanol extract ameliorates TNF-α/IFN-γ-induced inflammation in human keratinocytes and TPA-induced ear edema in mice

2020 ◽  
pp. 100831
Author(s):  
Eui Jeong Han ◽  
Hyun-Soo Kim ◽  
Kyungsook Jung ◽  
Kalu Kapuge Asanka Sanjeewa ◽  
Kalahe Hewage Iresha Nadeeka Madushani Herath ◽  
...  
Keyword(s):  
Ethanol Extract ◽  
Human Keratinocytes ◽  
Sargassum Horneri ◽  
Ear Edema ◽  
Tnf Α ◽  
Ifn Γ

Oral administration of Uncariae rhynchophylla inhibits the development of DNFB-induced atopic dermatitis-like skin lesions via IFN-γ down-regulation in NC/Nga mice

2009 ◽  
Vol 122 (3) ◽  
pp. 567-572 ◽  
Author(s):  
Dong-Young Kim ◽  
Jung-A Jung ◽  
Tae-Ho Kim ◽  
Sang-Wan Seo ◽  
Sung-Ki Jung ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Oral Administration ◽  
Skin Lesions ◽  
Down Regulation ◽  
Ifn Γ

scholarly journals Oral administration of Bifidobacterium longum CECT 7347 ameliorates gliadin-induced alterations in liver iron mobilisation

2013 ◽  
Vol 110 (10) ◽  
pp. 1828-1836 ◽  
Author(s):  
José Moisés Laparra ◽  
Marta Olivares ◽  
Yolanda Sanz
Keyword(s):  
Oral Administration ◽  
Intestinal Inflammation ◽  
Bifidobacterium Longum ◽  
Autoimmune Disorder ◽  
Mucosal Damage ◽  
Liver Iron ◽  
Expression Levels ◽  
Tnf Α ◽  
Ifn Γ ◽  
Hb Concentration

Coeliac disease is an autoimmune disorder triggered by gluten intake, causing intestinal inflammation and mucosal damage commonly associated with the malabsorption of nutrients and ferropenic anaemia. The present study evaluates the effects of the oral administration of Bifidobacterium longum CECT 7347 on gliadin-mediated alterations in hepatic Fe deposition and Hb concentration, liver transferrin receptor (TfR)-2, IL-6, TNF-α and hepcidin (Hamp) expression (mRNA), and active hepcidin peptide production by liquid chromatography–MS/MS. Weanling rats, sensitised or not with interferon (IFN)-γ, were fed with gliadins and/or the bifidobacterial strain. Gliadin feeding increased hepatic Fe deposition; however, only gliadin-fed sensitised animals showed lower Hb concentrations than the controls. TfR2 expression decreased after gliadins were fed to both sensitised and non-sensitised animals, and restored by the administration of B. longum. These observations were accompanied by increases in IL-6 expression levels in all the treatment groups; however, TNF-α expression only increased significantly in animals fed gliadins alone or together with B. longum if they had previously been sensitised with IFN-γ. Liver expression levels of Hamp diminished in all cases to the lowest values in animals sensitised with IFN-γ after being fed with gliadins and/or bifidobacteria. In these animals, plasma Hamp active peptide concentrations significantly increased when compared with the controls. Significant correlations were calculated between Hamp expression and liver Fe contents (liver Fe = 1/0·0032+0·032 × Hampexp), and Hb concentrations (Hb = 11·49+10·13 × (Hampexp)1/2). These data indicate that oral administration of B. longum ameliorates gliadin-mediated perturbations in liver Fe deposition and mobilisation.

scholarly journals Mixture of Polyphenols and Anthocyanins fromVaccinium uliginosum L.Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice

2012 ◽  
Vol 2012 ◽  
pp. 1-15 ◽  
Author(s):  
Min Ju Kim ◽  
Se-Young Choung
Keyword(s):  
Atopic Dermatitis ◽  
Oral Administration ◽  
Clinical Signs ◽  
Inflammatory Cells ◽  
Biological Properties ◽  
Dependent Manner ◽  
Protective Effects ◽  
Vaccinium Uliginosum ◽  
Skin Photoaging ◽  
Th1 Cytokine

Vaccinium uliginosum L. (VU) possesses various biological properties, such as antioxidant and protective effects against VU-induced skin photoaging. The purpose of this study is to evaluate the effects of oral administration of a mixture of polyphenols and anthocyanins derived from VU on 2,4-dinitrochlorobenzene- (DNCB-) induced atopic dermatitis (AD) in NC/Nga mice. We assessed anti-AD effects in NC/Nga murine model for a period of 9 weeks. Oral administration of the mixture significantly alleviated the AD-like skin symptoms and clinical signs including ear thickness and scratching behaviors. Orally administrated mixture reduced the level of IgE and IgG1, whereas it increased the level of IgG2a in a dose-dependent manner. The calculated IgG1/IgG2a ratio for each mouse revealed that the mixture derived from VU also significantly reduced the Th2/Th1 ratio, IL-4 and IL-13 (as Th2 cytokines), IFN-γ, and IL-12 (as a Th1 cytokine) in spleens. In addition, it significantly decreased gene expression, such as IL-4, IL-5, CCR3, eotaxin-1, IL- 12, IFN-γ, MCP-1, and IL-17, in AD-like lesions and suppressed Th17. Histological analyses revealed that the epidermis thickness and number of inflammatory cells were significantly reduced. In conclusion, oral administration of the mixture in the DNCB-induced AD is confirmed to improve AD disease in mice.

scholarly journals The extracellular serine protease from Staphylococcus epidermidis elicits a type 2 immune response in atopic dermatitis patients

2021 ◽  
Author(s):  
Goran Abdurrahman ◽  
Rebecca Pospich ◽  
Leif Steil ◽  
Manuela Gesell Salazar ◽  
Juan Izquierdo González ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Immune Response ◽  
T Cells ◽  
Atopic Dermatitis ◽  
Serine Protease ◽  
Staphylococcus Epidermidis ◽  
Type 2 Immune Response ◽  
Ifn Γ ◽  
Extracellular Serine Protease

Background: Atopic dermatitis (AD) is a chronic inflammatory skin disease caused by skin barrier defects and a misdirected type 2 immune response against antigens. The skin microbiome in AD is characterised by a reduction in microbial diversity with a dominance of staphylococci, including Staphylococcus epidermidis (S. epidermidis). To assess whether S. epidermidis antigens play a role in AD, we studied the immune response against the extracellular serine protease (Esp). Methods: We analyzed the binding of human IgG4 to S. epidermidis extracellular proteins using immunoblotting and mass spectrometry. We then measured serum antibodies specific for recombinant Esp by ELISA in healthy and AD individuals. We also stimulated T cells from AD patients and control subjects with Esp and measured the secreted cytokines. Finally, we analyzed the proteolytic activity of Esp against IL-33 and determined the cleavage sites by mass spectrometry. Results: We identified Esp as the dominant IgG4-binding antigen of S. epidermidis. Esp-specific IgE was present in human serum; AD patients had higher concentrations than controls. The T cell response to Esp in healthy adults was characterized by IL-17, IL-22, IFN-γ, and IL-10, whereas the AD patients’ T cells lacked IL-17 production and released only low amounts of IL-22, IFN-γ, and IL-10. In contrast, Th2 cytokine release was higher in T cells from AD patients than from healthy controls. Mature Esp cleaved and activated the alarmin IL-33. Conclusions: Esp elicits type 2-biased response in AD patients. This suggests that S. epidermidis can aggravate AD through the allergenic properties of Esp.

scholarly journals In Ovo and Oral Administration of Probiotic Lactobacilli Modulate Cell- and Antibody-Mediated Immune Responses in Newly Hatched Chicks

2020 ◽  
Author(s):  
Mohammadali Alizadeh ◽  
Jegarubee Bavananthasivam ◽  
Bahram Shojadoost ◽  
Jake Astill ◽  
Khaled Abdelaziz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune Response ◽  
Oral Administration ◽  
Keyhole Limpet Hemocyanin ◽  
Bursa Of Fabricius ◽  
Cytokine Gene Expression ◽  
Composition Analysis ◽  
In Ovo ◽  
Ifn Γ ◽  
Probiotic Lactobacilli

Abstract There is some evidence that lactobacilli can strengthen immune system of chickens. This study evaluated the effects of in ovo and oral administration of a lactobacilli cocktail on cytokine gene expression, antibody-mediated immune response, and spleen cellularity in chickens. Lactobacilli were administered either in ovo at embryonic day 18, orally to hatched chicks at days 1, 7, 14, 21, and 28 post-hatches, or by both treatments combined. On days 5 and 10 post-hatch, spleen and bursa of Fabricius were collected for gene expression and cell composition analysis. On days 14 and 21 post-hatch, birds were injected with sheep red blood cells (SRBC) and keyhole limpet hemocyanin (KLH), and sera were collected on days 7, 14, and 21 post-primary immunization. Birds that received lactobacilli (107 CFU) via in ovo followed by weekly oral administration showed a greater immune response by enhancing antibody response, increasing the percentage of CD4+ and CD4+CD25+ T cells in the spleen, and upregulating the expression of the expression of interferon (IFN)-α, IFN-β, interleukin (IL)-12, and IL-13 in the spleen and expression of IFN-γ, IL-2, IL-6, IL-8, IL-12, and IL-18 in bursa. These findings suggest that pre-and post-hatch administration of lactobacilli modulate immune response in newly hatched chickens.

Effects of Intercellular Communication and Stochasticity in Gene Expression On TH1/TH2 Differentiation.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 2673-2673
Author(s):  
Shlomit Reich-Zeliger ◽  
Yaron Emanuel Antebi ◽  
Jacob Rimer ◽  
Nir Friedmann
Keyword(s):  
Immune Response ◽  
Single Cell ◽  
Intercellular Communication ◽  
Experimental Results ◽  
Regulatory Mechanisms ◽  
Single Cell Level ◽  
Differentiation Process ◽  
Cell Level ◽  
Expression Levels ◽  
Ifn Γ

Abstract Abstract 2673 Poster Board II-649 Cytokines provide a main regulatory mechanism of a developing immune response, which allows for intercellular communication. This sharing of information enables cells to make collective decisions based on the state of the system rather than that of a single cell. In our research we focus on several examples of cytokine mediated regulatory mechanisms that function in helper T-cells (Th), whose role is to direct the immune response in the desired course by affecting responses of other cells. To analyze the relative importance of the different components affecting the behavior of the cells we employ mathematical modeling and numerical simulations of specific experimental settings. Quantitative experiments are performed to evaluate unknown parameters and to verify model predictions. We focus on two examples of cytokine mediated regulatory mechanisms that function in the helper T-cell (Th) differentiation. IL-12 and IL-4 direct differentiation into the main fates of Th cells, Th1 and Th2, respectively. By exposing cells to varied combinations of the two cytokines we experimentally map the “decision space” of the differentiation process. We measured, at the single cell level, eight key parameters relating to the state of the cells, from which we can deduce properties of the network describing the system. Our data indicate that under certain mixed conditions, with cells exposed to both IL-12 and IL-4, a new class of Th cells is observed, which secret both IL-4 and IFN-γ upon restimulation. This is preceded by co-expression of the two lineage specific transcription factors T-bet and GATA3 at earlier time points. We incorporate our experimental results and other known data into a cellular automata based simulation. Each cell is simulated as a node on a dynamic network, whose links describe the propagation of intercellular cytokine signals. The intracellular protein network for each node is realized as an automaton with cytokines levels as inputs and their secretion rates as output. By varying model parameters and comparing to experimental results we can gain insight into the significance of the various components driving the differentiation process. We also analyze the roles of heterogeneity in expression levels of cytokine receptors in the differentiation decision. To that end, naïve mice CD4 cells were sorted into separate groups according to their expression level of the IL-4 receptor. The different groups of cells were cultured separately, under “controlled milieu” conditions, at different IL-4 or IL-12 concentrations. Expression levels of key proteins involved in the differentiation process were measured at the single cell level every 24h. This was compared to expression levels of those proteins in a whole population of cells growing together. Our results reveal differences in the differentiation abilities between the sorted, controlled milieu, groups. Additionally, the weighted average of expression levels of the controlled milieu populations is different than that of the full heterogeneous population of cells growing together. These differences are caused by intercellular interactions, as cells secrete cytokines which are involved in and drive the differentiation process (e.g. IL-4, IFN-γ). Thus, these experiments provide an opportunity to evaluate the importance and roles played by intercellular communication in this process, and to asses what are the effects of stochastic levels of a protein (IL-4R in this case) on the following differentiation process. Disclosures: No relevant conflicts of interest to declare.

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