scholarly journals Mixture of Polyphenols and Anthocyanins fromVaccinium uliginosum L.Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice

2012 ◽  
Vol 2012 ◽  
pp. 1-15 ◽  
Author(s):  
Min Ju Kim ◽  
Se-Young Choung
Keyword(s):  
Atopic Dermatitis ◽  
Oral Administration ◽  
Clinical Signs ◽  
Inflammatory Cells ◽  
Biological Properties ◽  
Dependent Manner ◽  
Protective Effects ◽  
Vaccinium Uliginosum ◽  
Skin Photoaging ◽  
Th1 Cytokine

Vaccinium uliginosum L. (VU) possesses various biological properties, such as antioxidant and protective effects against VU-induced skin photoaging. The purpose of this study is to evaluate the effects of oral administration of a mixture of polyphenols and anthocyanins derived from VU on 2,4-dinitrochlorobenzene- (DNCB-) induced atopic dermatitis (AD) in NC/Nga mice. We assessed anti-AD effects in NC/Nga murine model for a period of 9 weeks. Oral administration of the mixture significantly alleviated the AD-like skin symptoms and clinical signs including ear thickness and scratching behaviors. Orally administrated mixture reduced the level of IgE and IgG1, whereas it increased the level of IgG2a in a dose-dependent manner. The calculated IgG1/IgG2a ratio for each mouse revealed that the mixture derived from VU also significantly reduced the Th2/Th1 ratio, IL-4 and IL-13 (as Th2 cytokines), IFN-γ, and IL-12 (as a Th1 cytokine) in spleens. In addition, it significantly decreased gene expression, such as IL-4, IL-5, CCR3, eotaxin-1, IL- 12, IFN-γ, MCP-1, and IL-17, in AD-like lesions and suppressed Th17. Histological analyses revealed that the epidermis thickness and number of inflammatory cells were significantly reduced. In conclusion, oral administration of the mixture in the DNCB-induced AD is confirmed to improve AD disease in mice.

scholarly journals Anti-Atopic Dermatitis Effect of Seaweed Fulvescens Extract via Inhibiting the STAT1 Pathway

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Tae-Young Gil ◽  
Yun-Mi Kang ◽  
Ye-Jin Eom ◽  
Chul-Hee Hong ◽  
Hyo-Jin An
Keyword(s):  
Atopic Dermatitis ◽  
Proinflammatory Cytokines ◽  
Inflammatory Cells ◽  
Control Group ◽  
Therapeutic Effects ◽  
Dependent Manner ◽  
Hacat Keratinocytes ◽  
Cellular Inflammation ◽  
Dose Dependent ◽  
Necrosis Factor

Seaweed fulvescens (SF) is a green alga rich in chlorophyll with unique flavor and taste. It is also called Maesaengi which has antioxidant and other physiological activities. In the present study, we evaluated the therapeutic effects of SF in a mouse model of Dermatophagoides farinae body-induced atopic dermatitis (AD) and in tumor necrosis factor-α and interferon-γ-stimulated HaCaT keratinocytes. SF treatment (200 mg/mouse) inhibited the development of AD symptoms, compared to that in the control group, as evidenced from the improved dorsal skin lesion, reduced thickness and infiltration of inflammatory cells and smaller lymph nodes, and reduced levels of proinflammatory cytokines. In HaCaT keratinocytes, SF (10, 25, and 50 μg/mL) suppressed the production of proinflammatory cytokines in a dose-dependent manner. In addition, SF reduced the phosphorylation of signal transducer and activator of transcription 1, which is one of the major signaling molecules involved in cellular inflammation. These results suggested that SF could be a potential therapeutic alternative for the treatment of AD.

Potent Inhibitory Effects of Quercetin on Inflammatory Responses of Collagen-Induced Arthritis in Mice

2019 ◽  
Vol 19 (3) ◽  
pp. 308-315 ◽  
Author(s):  
Kiichiro Kawaguchi ◽  
Masahiro Kaneko ◽  
Ryo Miyake ◽  
Hiroaki Takimoto ◽  
Yoshio Kumazawa
Keyword(s):  
Oral Administration ◽  
Inflammatory Responses ◽  
Inflammatory Cells ◽  
Knee Joints ◽  
Therapeutic Effects ◽  
Dependent Manner ◽  
Inhibitory Effects ◽  
Collagen Induced Arthritis ◽  
Tnf Α ◽  
Flavonoid Quercetin

Background: Production of tumor necrosis factor (TNF)-α by inflammatory cells in lesions is the hallmark of the pathogenesis of rheumatoid arthritis (RA). Regulation of inflammatory responses in knee joints of patients with RA is critical for improving severe symptoms. Flavonoids have inhibitory effects on the acute and chronic inflammatory responses caused by TNF-α. The flavonoid quercetin (QUER) is one of the most prominent dietary antioxidants. Objective: The present study investigated the preventive and therapeutic effects of QUER on inflammatory responses in collagen-induced arthritis (CIA) in mice. Methods: Mice with CIA, a mouse model for RA, were treated with QUER orally three times a week either from the second immunization with collagen (day 21) or day 28 when symptoms of CIA had developed midway. Results: In both cases, inflammation-related clinical scores of knee joints were significantly reduced by treatment with QUER. Histological analyses showed that the representative characteristics of RA, such as damage to interchondral joints, infiltration of inflammatory cells, and pannus formation, were significantly reduced by QUER treatment. Oral administration of QUER significantly decreases lipopolysaccharide (LPS)-induced TNF-α production in a dose-dependent manner. Expression of TNF- α mRNA in knee joints was decreased in QUER-treated mice, compared with those of CIA controls. Conclusion: These results suggest that oral administration of QUER might effectively improve symptoms of RA.

scholarly journals Metal Chelating, Inhibitory DNA Damage, and Anti-Inflammatory Activities of Phenolics from Rambutan (Nephelium lappaceum) Peel and the Quantifications of Geraniin and Corilagin

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2263 ◽  
Author(s):  
Yujing Li ◽  
Zhaojie Li ◽  
Hu Hou ◽  
Yongliang Zhuang ◽  
Liping Sun
Keyword(s):  
Dna Damage ◽  
Cell Model ◽  
Biological Properties ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Dependent Manner ◽  
Protective Effects ◽  
Metal Chelating ◽  
Study Results ◽  
Anti Inflammatory

Whereas the preparation and biological properties of rambutan peel phenolics (RPP) were explored in our previous studies, the metal chelating, inhibitory DNA damage, and anti-inflammatory activities of RPP were evaluated and the important phenolics of RPP quantified in this study. Results showed that RPP had high Fe2+ and Cu2+-chelating activities with EC50 of 0.80 mg/mL and 0.13 mg/mL, respectively. RPP effectively decreased the production of hydroxyl radical with IC50 of 62.4 μg/mL. The protective effects of RPP against AAPH-induced DNA damage were also explored. RPP efficiently inhibited peroxyl radical-induced plasmid DNA strand breakage. The anti-inflammatory effects of RPP were determined using a lipopolysaccharide (LPS)-induced RAW 264.7 cell model. RPP significantly inhibited the production of nitric oxide (NO) and controlled the levels of inducible NO synthase mRNA in LPS-induced RAW 264.7 cells. The inhibitory activity increased in a dose-dependent manner. The above bioactivity of RPP was associated with its phenolic content and phenolic profiles. Furthermore, the contents of geraniin and corilagin in RPP were determined by an ultra-high performance liquid chromatography coupled with triple quadruple mass spectrometry (UPLC-QQQ-MS), showing 140.02 and 7.87 mg/g extract dry weight. Thus, RPP has potential applications as a novel nutraceutical and functional food in health promotion.

scholarly journals Oral Administration of Sargassum horneri Improves the HDM/DNCB-Induced Atopic Dermatitis in NC/Nga Mice

Nutrients ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2482 ◽  
Author(s):  
Eui Jeong Han ◽  
Ilekuttige Priyan Shanura Fernando ◽  
Hyun-Soo Kim ◽  
You-Jin Jeon ◽  
Dissanayaka Mudiyanselage Dinesh Madusanka ◽  
...  
Keyword(s):  
Immune Response ◽  
Atopic Dermatitis ◽  
Oral Administration ◽  
Ethanol Extract ◽  
Transepidermal Water Loss ◽  
Sargassum Horneri ◽  
Protective Effects ◽  
Expression Levels ◽  
Abnormal Immune Response ◽  
Ifn Γ

The present study investigated the protective effects of Sargassum horneri (S. horneri) ethanol extract (SHE) against atopic dermatitis (AD), known as an abnormal immune response in house dust mite (HDM)/2,4-dinitrochlorobenzene (DNCB)-stimulated NC/Nga mice. The oral administration of SHE attenuated the AD symptoms, including the skin dermatitis severity, transepidermal water loss (TEWL), and ear edema in HDM/DNCB-stimulated mice. Moreover, the histological analysis revealed that SHE improved epidermal hyperplasia and hyperkeratosis, and reduced the dermal infiltrations of mast cells and eosinophils. Moreover, SHE downregulated the expression levels of cytokines (interleukin (IL)-6, IL-10, and interferon (IFN)-γ) and chemokines (Regulated on Activation, Normal T Cell Expressed and Secreted (RANTES), Eotaxin, and Thymus and activation-regulated chemokine (TARC)) by decreasing the expression levels of atopic initiators (IL-25 and IL-33) in HDM/DNCB-stimulated skin. The oral administration of SHE decreased the spleen size, reducing expression levels of AD-related cytokines (IL-4, IL-5, IL-6, IL-10, IL-13, IFN-γ, and TARC) by regulating the expressions of Tbx21 (T-bet), GATA Binding Protein 3 (GATA-3), and Signal transducer and activator of transcription 3 (STAT3). Moreover, SHE significantly attenuated the serum immunoglobulin (Ig)G1 and IgG2a levels in HDM/DNCB-stimulated mice. Collectively, these results suggest that S. horneri could be an ingredient of functional food against abnormal immune response.

scholarly journals Protective Effects of Kyungohkgo on Atopic Dermatitis in HaCaT Cells

2021 ◽  
Vol 27 (5) ◽  
pp. 1286-1292
Author(s):  
Il-Joo Jo ◽  
Mee-Ok Choi
Keyword(s):  
Atopic Dermatitis ◽  
Mrna Levels ◽  
Dependent Manner ◽  
Hacat Cells ◽  
Protective Effects ◽  
Concentration Dependent Manner ◽  
Tnf Α ◽  
Anti Oxidant ◽  
Stat1 Signaling ◽  
Ifn Γ

Kyungohkgo (KOG) is an oriental herbal medicine that has been used for its various pharmacological effects, which include anti-oxidant, anti-inflammatory and immuno-regulation activities. But its effects and mechanisms of anti-atopic dermatitis (AD) have not been elucidated. HaCaT cells were pre-treated with KOG for 1 h and stimulated with tumor necrosis factor (TNF)-α and interferon (IFN)-γ (10 ng/ml each). After 24 h, cells were harvested to measure the production of reactive oxygen species (ROS) and chemokines such as regulated upon activation, normal T cell expressed and secreted (RANTES/CCL5), Thymus and activation-regulated chemokine (TARC/CCL17) and macrophage-deraived chemokine (MDC/CCL22). To investigate the regulatory mechanisms of KOG, we also assessed the phosphorylation of signal transducer and activator of transcription (STAT1) signaling pathways in HaCaT cells. Treatment of KOG decreased the ROS production and mRNA levels of RANTES, TARC, MDC with a concentration dependent manner. In addition, KOG significantly inhibited TNF-α and IFN-γ induced phosphorylation of STAT1. This could indicate that the KOG shows anti-AD activity mainly through STAT1. Thus, we propose that KOG may be a promising anti-AD skin protector, which could suggest the clinical basis for cosmetics development.

scholarly journals Effectiveness of the Novel Herbal Medicine, KIOM-MA, and Its Bioconversion Product, KIOM-MA128, on the Treatment of Atopic Dermatitis

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Tae Ho Chung ◽  
Tae Jin Kang ◽  
Won-Kyung Cho ◽  
Ga Young Im ◽  
Geum Seon Lee ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Herbal Medicine ◽  
Oral Administration ◽  
Aluminum Hydroxide ◽  
Lactobacillus Acidophilus ◽  
Hplc Analysis ◽  
Clinical Signs ◽  
The Novel ◽  
Fermented Product ◽  
Therapeutic Properties

This study was conducted to determine if oral administration of the novel herbal medicine, KIOM-MA, and itsLactobacillus acidophilus-fermented product, KIOM-MA128, has therapeutic properties for the treatment of atopic dermatitis (AD). Using AD-induced BALB/c mice by Ovalbumin and aluminum hydroxide, the effectiveness of KIOM-MA and KIOM-MA128 on AD was evaluated. Oral administration of KIOM-MA and KIOM-MA128 reduced major clinical signs of AD including erythema/darkening, edema/papulation, excoriations, lichenification/prurigo, and dryness. Interestingly, KIOM-MA128 more significantly improved AD-related symptoms including decrease of IgE level in the plasma as well as reduction of scratching behavior, skin severity in the AD BALB/c model. HPLC analysis showed the significant changes in the constituent patterns between KIOM-MA and KIOM-MA128. Our results suggest that both KIOM-MA and KIOM-MA128 have potential for therapeutic reagent for the treatment of AD, and further, the efficacy is significantly enhanced byL. acidophilusfermentation via increases in its indicator molecule.

scholarly journals Repeated oral administration of low doses of silver in mice: tissue distribution and effects on central nervous system

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Camilla Recordati ◽  
Marcella De Maglie ◽  
Claudia Cella ◽  
Simona Argentiere ◽  
Saverio Paltrinieri ◽  
...  
Keyword(s):  
Oral Administration ◽  
Tissue Distribution ◽  
Clinical Chemistry ◽  
Kidney Tissue ◽  
Clinical Signs ◽  
Oral Exposure ◽  
Dependent Manner ◽  
Low Doses ◽  
Dose Dependent ◽  
The Brain

Abstract Background Widespread use of silver in its different forms raises concerns about potential adverse effects after ingestion, the main exposure route for humans. The aim of this study was to investigate in CD-1 (ICR) male mice the tissue distribution and in vivo effects of 4-week oral exposure to 0.25 and 1 mg Ag/kg bw 10 nm citrate coated silver nanoparticles (AgNPs) and 1 mg Ag/kg bw silver acetate (AgAc) at the end of treatment (EoT) and after 4 weeks of recovery. Results There were no treatment-related clinical signs and mortality, and no significant effects on body and organ weights at the EoT and after recovery. Treatment-related changes in hematology and clinical chemistry were found after recovery, the most relevant being a dose-dependent lymphopenia and increased triglycerides in AgNP-treated mice, and increased levels of urea in all treated groups, associated with decreased albumin only in AgAc-treated mice. At the EoT the highest silver concentration determined by Triple Quadrupole ICP-MS analysis was found in the brain, followed by testis, liver, and spleen; much lower concentrations were present in the small intestine and kidney. Tissue silver concentrations were slightly higher after exposure to AgAc than AgNPs and dose dependent for AgNPs. After recovery silver was still present in the brain and testis, highlighting slow elimination. No histopathological changes and absence of silver staining by autometallography were observed in the organs of treated mice. At the EoT GFAP (astrocytes) immunoreactivity was significantly increased in the hippocampus of AgNP-treated mice in a dose-dependent manner and Iba1 (microglial cells) immunoreactivity was significantly increased in the cortex of 1 mg/kg bw AgNP-treated mice. After recovery, a significant reduction of Iba1 was observed in the cortex of all treated groups. TEM analysis of the hippocampus revealed splitting of basement membrane of the capillaries and swelling of astrocytic perivascular end-feet in 1 mg/kg bw AgNP- and AgAc-treated mice at the EoT. Conclusions Our study revealed accumulation and slow clearance of silver in the brain after oral administration of 10 nm AgNPs and AgAc at low doses in mice, associated with effects on glial cells and ultrastructural alterations of the Blood-Brain Barrier.

Healing effects of curcumin nanoparticles in deep tissue injury mouse model.

2020 ◽  
Vol 18 ◽  
Author(s):  
Zirui Zhang ◽  
Shangcong Han ◽  
Panpan Liu ◽  
Xu Yang ◽  
Jing Han ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mrna Expression ◽  
Tissue Injury ◽  
Inflammatory Cells ◽  
Pcr Analysis ◽  
Protective Effects ◽  
Deep Tissue ◽  
Deep Tissue Injury

Background: Chronic inflammation and lack of angiogenesis are the important pathological mechanisms in deep tissue injury (DTI). Curcumin is a well-known anti-inflammatory and antioxidant agent. However, curcumin is unstable under acidic and alkaline conditions, and can be rapidly metabolized and excreted in the bile, which shortens its bioactivity and efficacy. Objective: This study aimed to prepare curcumin-loaded poly (lactic-co-glycolic acid) nanoparticles (CPNPs) and to elucidate the protective effects and underlying mechanisms of wound healing in DTI models. Methods: CPNPs were evaluated for particle size, biocompatibility, in vitro drug release and their effect on in vivo wound healing. Results : The results of in vivo wound closure analysis revealed that CPNP treatments significantly improved wound contraction rates (p<0.01) at a faster rate than other three treatment groups. H&E staining revealed that CPNP treatments resulted in complete epithelialization and thick granulation tissue formation, whereas control groups resulted in a lack of compact epithelialization and persistence of inflammatory cells within the wound sites. Quantitative real-time PCR analysis showed that treatment with CPNPs suppressed IL-6 and TNF-α mRNA expression, and up-regulated TGF-β, VEGF-A and IL-10 mRNA expression. Western blot analysis showed up-regulated protein expression of TGF-β, VEGF-A and phosphorylatedSTAT3. Conclusion: Our results showed that CPNPs enhanced wound healing in DTI models, through modulation of the JAK2/STAT3 signalling pathway and subsequent upregulation of pro-healing factors.

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