scholarly journals Surface Properties and Adherence of Bradyrhizobium diazoefficiens to Glycine max Roots Are Altered When Grown in Soil Extracted Nutrients

Nitrogen ◽  
2021 ◽  
Vol 2 (4) ◽  
pp. 461-473
Author(s):  
Armaan Kaur Sandhu ◽  
Senthil Subramanian ◽  
Volker S. Brözel
Keyword(s):  
Surface Properties ◽  
Culture Media ◽  
Root Hairs ◽  
Soil Nutrient ◽  
Surface Hydrophobicity ◽  
Soft Agar ◽  
Laboratory Culture ◽  
Soil Environment ◽  
Bacterial Surface ◽  
Multiple Strains

Soybean roots are colonized and nodulated by multiple strains of compatible nitrogen-fixing rhizobia primarily belonging to the Genus Bradyrhizobium. Motility towards the root and attachment to root hairs are key determinants of competitive colonization and subsequent nodulation. Bacterial surface properties and motility are known to vary with chemical composition of the culture medium, and root adhesion and nodulation occur in a soil environment rather than laboratory medium. We asked whether the nodulation-promoting factors motility, surface hydrophobicity and surface adhesion of Bradyrhizobium are affected by growth in a soil nutrient environment. B. diazoefficiens USDA 110, 126, 3384, and B. elkanii USDA 26 were grown in mineral salt medium with peptone, yeast extract and arabinose (PSY), and in a soil extracted soluble organic matter (SESOM) medium. Surface hydrophobicity was determined by partitioning into hydrocarbon, motility by transition through soft agar, and surface-exposed saccharides by lectin profiling, followed by biofilm formation and soybean root adhesion capacity of populations. SESOM-grown populations were generally less motile and more hydrophobic. They bound fewer lectins than PSY-grown populations, indicating a simpler surface saccharide profile. SESOM populations of USDA 110 did not form detectable biofilm, but showed increased binding to soy roots. Our results indicate that growth in a soil environment impacts surface properties, motility, and subsequent soy root adhesion propensity. Hence, evaluation of Bradyrhizobium for nodulation efficiency should be performed using soil from the specific field where the soybeans are to be planted, rather than laboratory culture media.

SubMICs of penicillin and erythromycin enhance biofilm formation and hydrophobicity of Corynebacterium diphtheriae strains

2013 ◽  
Vol 62 (5) ◽  
pp. 754-760 ◽  
Author(s):  
D. L. R. Gomes ◽  
R. S. Peixoto ◽  
E. A. B. Barbosa ◽  
F. Napoleão ◽  
P. S. Sabbadini ◽  
...  
Keyword(s):  
Cell Surface ◽  
Surface Properties ◽  
Biofilm Formation ◽  
Antimicrobial Agents ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Corynebacterium Diphtheriae ◽  
Surface Charges ◽  
Bacterial Surface ◽  
Bacterial Morphology

Subinhibitory concentrations (subMICs) of antibiotics may alter bacterial surface properties and change microbial physiology. This study aimed to investigate the effect of a subMIC (⅛ MIC) of penicillin (PEN) and erythromycin (ERY) on bacterial morphology, haemagglutinating activity, cell-surface hydrophobicity (CSH) and biofilm formation on glass and polystyrene surfaces, as well as the distribution of cell-surface acidic anionic residues of Corynebacterium diphtheriae strains (HC01 tox − strain; CDC-E8392 and 241 tox + strains). All micro-organisms tested were susceptible to PEN and ERY. Growth in the presence of PEN induced bacterial filamentation, whereas subMIC of ERY caused cell-size reduction of strains 241 and CDC-E8392. Adherence to human erythrocytes was reduced after growth in the presence of ERY, while CSH was increased by a subMIC of both antibiotics in bacterial adherence to n-hexadecane assays. Conversely, antibiotic inhibition of biofilm formation was not observed. All strains enhanced biofilm formation on glass after treatment with ERY, while only strain 241 increased glass adherence after cultivation in the presence of PEN. Biofilm production on polystyrene surfaces was improved by ⅛ MIC of ERY. After growth in the presence of both antimicrobial agents, strains 241 and CDC-E8392 exhibited anionic surface charges with focal distribution. In conclusion, subMICs of PEN and ERY modified bacterial surface properties and enhanced not only biofilm formation but also cell-surface hydrophobicity. Antibiotic-induced biofilm formation may contribute to the inconsistent success of antimicrobial therapy for C. diphtheriae infections.

scholarly journals Changes in surface characteristics and adsorption properties of 2,4,6-trichlorophenol following Fenton-like aging of biochar

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Liqiang Cui ◽  
Qinya Fan ◽  
Jianxiong Sun ◽  
Guixiang Quan ◽  
Jinlong Yan ◽  
...  
Keyword(s):  
Adsorption Capacity ◽  
Surface Properties ◽  
Aging Process ◽  
Surface Characteristics ◽  
Natural Soil ◽  
Peanut Shell ◽  
Soil Environment ◽  
Polluted Soils ◽  
Physico Chemical

AbstractFenton-like system formed in a natural soil environment deemed to be significant in the aging process of biochar. Aged biochars have distinct physico-chemical and surface properties compared to non-aged biochar. The aged biochar proved to be useful soil amendment due to its improved elements contents and surface properties. The biochar aging process resulted in increased surface area and pore volume, as well as carbon and oxygen-containing functional groups (such as C=O, –COOH, O–C=O etc.) on its surface, which were also associated with the adsorption behavior of 2,4,6-trichlorophenol (2,4,6-TCP). The biochar aging increased the adsorption capacity of 2,4,6-TCP, which was maximum at pH 3.0. The 2,4,6-TCP adsorption capacity of aged-bush biochar (ABB) and aged-peanut shell biochar (APB) was increased by 1.0–11.0% and 7.4–38.8%, respectively compared with bush biochar (BB) and peanut shell biochar (PB) at the same initial concentration of 2,4,6-TCP. All biochars had similar 2,4,6-TCP desorption rates ranging from 33.2 to 73.3% at different sorption temperatures and times. The desorbed components were mainly 2,4,6-TCP and other degraded components, which were low in concentration with small molecule substance. The results indicated that the aged-biochar could be effective for the long-term remediation of naturally organic polluted soils.

The role of the interplay between polymer architecture and bacterial surface properties on the microbial adhesion to polyoxazoline-based ultrathin films

Biomaterials ◽  
2010 ◽  
Vol 31 (36) ◽  
pp. 9462-9472 ◽  
Author(s):  
Bidhari Pidhatika ◽  
Jens Möller ◽  
Edmondo M. Benetti ◽  
Rupert Konradi ◽  
Ekaterina Rakhmatullina ◽  
...  
Keyword(s):  
Surface Properties ◽  
Ultrathin Films ◽  
Polymer Architecture ◽  
Microbial Adhesion ◽  
Bacterial Surface

Dissociation of zinc phthalocyanine aggregation on bacterial surface is key for photodynamic antimicrobial effect

2018 ◽  
Vol 22 (09n10) ◽  
pp. 925-934 ◽  
Author(s):  
Dafeng Liu ◽  
Linsen Li ◽  
Jincan Chen ◽  
Zhuo Chen ◽  
Longguang Jiang ◽  
...  
Keyword(s):  
Antimicrobial Effect ◽  
Antibacterial Activities ◽  
Surface Hydrophobicity ◽  
The Other ◽  
Zinc Phthalocyanine ◽  
Bacterial Surface ◽  
E Coli ◽  
Bacterial Signaling ◽  
Bacterial Uptake ◽  
Key Parameter

Antimicrobial photodynamic therapy (aPDT) is an effective mean for killing bacteria in this era of increasing multi-antibiotic resistance, and possesses a number of unique advantages. Much effort has been devoted to the development a key component of aPDT photosensitizers (PSs). We synthesized a series of PSs with different positive charges (ZnPc(Lys)[Formula: see text], where [Formula: see text] 3, 5, 7, and studied their antibacterial activities and mechanisms against Escherichia coli (E. coli). Interestingly, the ZnPc(Lys)[Formula: see text] derivative showed stronger antibacterial effect (MIC = 25.3 [Formula: see text]M) than the other two PSs (MICs = 50.6 [Formula: see text]M), even though this PS did not have the highest uptake on bacteria among these PSs. It was ZnPc(Lys)[Formula: see text] that possessed the highest bacterial uptake. ZnPc(Lys)[Formula: see text] was found to have the highest monomeric fractions (62.0%) on bacteria surface than the other two PSs (37.9% for [Formula: see text] 3 and 33.9% [Formula: see text] = 7). These results clearly demonstrate that PS conformation on bacterial surface as a key parameter determining antibacterial efficacy of PSs. Other mechanistic aspects of photodynamic effects, including PS binding kinetics, bacterial surface hydrophobicity, zeta potential of bacteria, membrane permeability and bacterial signaling pathways were also studied.

scholarly journals Rapid sequencing of MRSA direct from clinical plates in a routine microbiology laboratory

2019 ◽  
Vol 74 (8) ◽  
pp. 2153-2156 ◽  
Author(s):  
Beth Blane ◽  
Kathy E Raven ◽  
Danielle Leek ◽  
Nicholas Brown ◽  
Julian Parkhill ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Clinical Laboratory ◽  
Culture Media ◽  
Direct Sequencing ◽  
Laboratory Culture ◽  
Outbreak Detection ◽  
Snp Analysis ◽  
University Hospitals ◽  
Quality Control Metrics ◽  
Clinical Culture

Abstract Background Routine sequencing of MRSA could bring about significant improvements to outbreak detection and investigation. Sequencing is commonly performed using DNA extracted from a pure culture, but overcoming the delay associated with this step could reduce the time to infection control interventions. Objectives To develop and evaluate rapid sequencing of MRSA using primary clinical cultures. Methods Patients with samples submitted to the clinical laboratory at the Cambridge University Hospitals NHS Foundation Trust from which MRSA was isolated were identified, the routine laboratory culture plates obtained and DNA extraction and sequencing performed. Results An evaluation of routine MRSA cultures from 30 patients demonstrated that direct sequencing from bacterial colonies picked from four different culture media was feasible. The 30 clinical MRSA isolates were sequenced on the day of plate retrieval over five runs and passed quality control metrics for sequencing depth and coverage. The maximum contamination detected using Kraken was 1.09% fragments, which were identified as Prevotella dentalis. The most common contaminants were other staphylococcal species (25 isolate sequences) and Burkholderia dolosa (11 isolate sequences). Core genome pairwise SNP analysis to identify clusters based on isolates that were ≤50 SNPs different was used to triage cases for further investigation. This identified three clusters, but more detailed genomic and epidemiological evaluation excluded an acute outbreak. Conclusions Rapid sequencing of MRSA from clinical culture plates is feasible and reduces the delay associated with purity culture prior to DNA extraction.

In situ detection of cell surface hydrophobicity of probe-defined bacteria in activated sludge

2001 ◽  
Vol 43 (6) ◽  
pp. 97-103 ◽  
Author(s):  
J. L. Nielsen ◽  
L. H. Mikkelsen ◽  
P. H. Nielsen
Keyword(s):  
Activated Sludge ◽  
Surface Properties ◽  
Treatment Plant ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Substrate Uptake ◽  
Filamentous Bacteria ◽  
In Situ Conditions ◽  
Bacterial Surfaces

The surface hydrophobicity of different types of bacteria in activated sludge were investigated under in situ conditions by following the adhesion of fluorescent microspheres with defined surface properties to bacterial surfaces (the MAC-method). This technique was combined with identification of the bacteria with fluorescence in situ hybridization with rRNA-targeted oligonucleotides (FISH) and could thus be used for characterization of surface properties of probe-defined bacteria directly in a complex system without prior enrichment or isolation. This MAC-FISH technique could be used for single bacteria as well as filamentous bacteria. In the investigated activated sludge from an industrial wastewater treatment plant, two types of filamentous bacteria dominated. One morphotype consistently attracted only very few hydrophobic microspheres, indicating that the thin sheath of exopolymers around the cells had a hydrophilic surface. Use of a hierarchical set of gene probes revealed that these filaments were sulphide oxidising Thiothrix spp. The other predominating filamentous morphotype had a thick, very hydrophobic exopolymeric sheath. This filamentous bacterium was found to belong to the alpha-Proteobacteria. The relevance of the significant differences in surface hydrophobicity for the two morphotypes in respect to substrate uptake and floc formation is discussed.

Importance of bacterial surface properties to control the stability of emulsions

2006 ◽  
Vol 112 (1) ◽  
pp. 26-34 ◽  
Author(s):  
Mai Huong Ly ◽  
Murielle Naïtali-Bouchez ◽  
Thierry Meylheuc ◽  
Marie-Noëlle Bellon-Fontaine ◽  
Thanh Mai Le ◽  
...  
Keyword(s):  
Surface Properties ◽  
Bacterial Surface ◽  
The Stability

Atomic Force Microscopy Measurement of Heterogeneity in Bacterial Surface Hydrophobicity

Langmuir ◽  
2008 ◽  
Vol 24 (9) ◽  
pp. 4944-4951 ◽  
Author(s):  
Loredana S. Dorobantu ◽  
Subir Bhattacharjee ◽  
Julia M. Foght ◽  
Murray R. Gray
Keyword(s):  
Atomic Force Microscopy ◽  
Surface Hydrophobicity ◽  
Atomic Force Microscopy Measurement ◽  
Bacterial Surface ◽  
Force Microscopy ◽  
Atomic Force ◽  
Microscopy Measurement

scholarly journals Mosaic-CLSM Assessment of Bacterial Spatial Distribution in Cosmetic Matrices According to Matrix Viscosity and Bacterial Hydrophobicity

Cosmetics ◽  
2020 ◽  
Vol 7 (2) ◽  
pp. 32
Author(s):  
Samia Almoughrabie ◽  
Chrisse Ngari ◽  
Romain Briandet ◽  
Valérie Poulet ◽  
Florence Dubois-Brissonnet
Keyword(s):  
Spatial Distribution ◽  
Laser Scanning ◽  
Rapid Assessment ◽  
Challenge Test ◽  
Surface Hydrophobicity ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Bacterial Surface ◽  
Bacterial Hydrophobicity ◽  
The Impact

The reliability of the challenge test depends, among other parameters, on the spatial distribution of microorganisms in the matrix. The present study aims to quickly identify factors that are susceptible to impair a uniform distribution of inoculated bacteria in cosmetic matrices in this context. We used mosaic confocal laser scanning microscopy (M-CLSM) to obtain rapid assessment of the impact of the composition and viscosity of cosmetic matrices on S. aureus spatial distribution. Several models of cosmetic matrices were formulated with different concentrations of two thickeners and were inoculated with three S. aureus strains having different levels of hydrophobicity. The spatial distribution of S. aureus in each matrix was evaluated according to the frequency distribution of the fluorescence values of at least 1350 CLSM images. We showed that, whatever the thickener used, an increasingly concentration of thickener results in increasingly bacterial clustered distribution. Moreover, higher bacterial hydrophobicity also resulted in a more clustered spatial distribution. In conclusion, CLSM-based method allows a rapid characterization of bacterial spatial distribution in complex emulsified systems. Both matrix viscosity and bacterial surface hydrophobicity affect the bacterial spatial distribution which can have an impact on the reliability of bacterial enumeration during challenge test.

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