Reaction of Opalinas to Various Laboratory Culture Media

1928 ◽  
Vol 47 (1) ◽  
pp. 1 ◽  
Author(s):  
Mary E. Larson
Keyword(s):  
Culture Media ◽  
Laboratory Culture

scholarly journals Rapid sequencing of MRSA direct from clinical plates in a routine microbiology laboratory

2019 ◽  
Vol 74 (8) ◽  
pp. 2153-2156 ◽  
Author(s):  
Beth Blane ◽  
Kathy E Raven ◽  
Danielle Leek ◽  
Nicholas Brown ◽  
Julian Parkhill ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Clinical Laboratory ◽  
Culture Media ◽  
Direct Sequencing ◽  
Laboratory Culture ◽  
Outbreak Detection ◽  
Snp Analysis ◽  
University Hospitals ◽  
Quality Control Metrics ◽  
Clinical Culture

Abstract Background Routine sequencing of MRSA could bring about significant improvements to outbreak detection and investigation. Sequencing is commonly performed using DNA extracted from a pure culture, but overcoming the delay associated with this step could reduce the time to infection control interventions. Objectives To develop and evaluate rapid sequencing of MRSA using primary clinical cultures. Methods Patients with samples submitted to the clinical laboratory at the Cambridge University Hospitals NHS Foundation Trust from which MRSA was isolated were identified, the routine laboratory culture plates obtained and DNA extraction and sequencing performed. Results An evaluation of routine MRSA cultures from 30 patients demonstrated that direct sequencing from bacterial colonies picked from four different culture media was feasible. The 30 clinical MRSA isolates were sequenced on the day of plate retrieval over five runs and passed quality control metrics for sequencing depth and coverage. The maximum contamination detected using Kraken was 1.09% fragments, which were identified as Prevotella dentalis. The most common contaminants were other staphylococcal species (25 isolate sequences) and Burkholderia dolosa (11 isolate sequences). Core genome pairwise SNP analysis to identify clusters based on isolates that were ≤50 SNPs different was used to triage cases for further investigation. This identified three clusters, but more detailed genomic and epidemiological evaluation excluded an acute outbreak. Conclusions Rapid sequencing of MRSA from clinical culture plates is feasible and reduces the delay associated with purity culture prior to DNA extraction.

Laboratory culture media and enzyme activity of some entomopathogenic fungi of Zoophthora (Zygomycetes: Entomophthoraceae)

1992 ◽  
Vol 59 (3) ◽  
pp. 250-257 ◽  
Author(s):  
Marek J. Urbańczyk ◽  
Andrzej Zabża ◽  
Stanisław Bałazy ◽  
Wanda Peczyńska-Czoch
Keyword(s):  
Enzyme Activity ◽  
Entomopathogenic Fungi ◽  
Culture Media ◽  
Laboratory Culture

scholarly journals Surface Properties and Adherence of Bradyrhizobium diazoefficiens to Glycine max Roots Are Altered When Grown in Soil Extracted Nutrients

Nitrogen ◽  
2021 ◽  
Vol 2 (4) ◽  
pp. 461-473
Author(s):  
Armaan Kaur Sandhu ◽  
Senthil Subramanian ◽  
Volker S. Brözel
Keyword(s):  
Surface Properties ◽  
Culture Media ◽  
Root Hairs ◽  
Soil Nutrient ◽  
Surface Hydrophobicity ◽  
Soft Agar ◽  
Laboratory Culture ◽  
Soil Environment ◽  
Bacterial Surface ◽  
Multiple Strains

Soybean roots are colonized and nodulated by multiple strains of compatible nitrogen-fixing rhizobia primarily belonging to the Genus Bradyrhizobium. Motility towards the root and attachment to root hairs are key determinants of competitive colonization and subsequent nodulation. Bacterial surface properties and motility are known to vary with chemical composition of the culture medium, and root adhesion and nodulation occur in a soil environment rather than laboratory medium. We asked whether the nodulation-promoting factors motility, surface hydrophobicity and surface adhesion of Bradyrhizobium are affected by growth in a soil nutrient environment. B. diazoefficiens USDA 110, 126, 3384, and B. elkanii USDA 26 were grown in mineral salt medium with peptone, yeast extract and arabinose (PSY), and in a soil extracted soluble organic matter (SESOM) medium. Surface hydrophobicity was determined by partitioning into hydrocarbon, motility by transition through soft agar, and surface-exposed saccharides by lectin profiling, followed by biofilm formation and soybean root adhesion capacity of populations. SESOM-grown populations were generally less motile and more hydrophobic. They bound fewer lectins than PSY-grown populations, indicating a simpler surface saccharide profile. SESOM populations of USDA 110 did not form detectable biofilm, but showed increased binding to soy roots. Our results indicate that growth in a soil environment impacts surface properties, motility, and subsequent soy root adhesion propensity. Hence, evaluation of Bradyrhizobium for nodulation efficiency should be performed using soil from the specific field where the soybeans are to be planted, rather than laboratory culture media.

scholarly journals Two Listeria monocytogenes Pseudo-outbreaks Caused by Contaminated Laboratory Culture Media

2015 ◽  
Vol 54 (3) ◽  
pp. 768-770 ◽  
Author(s):  
Almea Matanock ◽  
Lee S. Katz ◽  
Kelly A. Jackson ◽  
Zuzana Kucerova ◽  
Amanda R. Conrad ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Pregnant Women ◽  
Culture Media ◽  
Laboratory Culture ◽  
Body Fluids ◽  
Elderly Adults ◽  
Content Type ◽  
Products Of Conception ◽  
Foodborne Infection ◽  
Sterile Body Fluids

Listeriosis is a serious foodborne infection that disproportionately affects elderly adults, pregnant women, newborns, and immunocompromised individuals. Diagnosis is made by culturingListeria monocytogenesfrom sterile body fluids or from products of conception. This report describes the investigations of two listeriosis pseudo-outbreaks caused by contaminated laboratory media made from sheep blood.

scholarly journals Susceptibility of Escherichia coli to L-selenaproline and other L-proline analogues in laboratory culture media and normal human urine

2006 ◽  
Vol 43 (4) ◽  
pp. 392-398 ◽  
Author(s):  
C.E. Deutch ◽  
M.E. Arballo ◽  
L.N. Cooks ◽  
J.M. Gomes ◽  
T.M. Williams ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Human Urine ◽  
Culture Media ◽  
Laboratory Culture ◽  
Normal Human

Improved Medium for Red Halophilic Bacteria from Salt Fish

1952 ◽  
Vol 9 (3) ◽  
pp. 157-163 ◽  
Author(s):  
H. P. Dussault ◽  
R. A. Lachance
Keyword(s):  
Incubation Time ◽  
Culture Media ◽  
Agar Medium ◽  
Halophilic Bacteria ◽  
Skim Milk ◽  
Laboratory Culture ◽  
Proteose Peptone ◽  
Slow Development ◽  
Fish Samples ◽  
Stimulation Of

The relatively slow development of red halophilic bacteria on laboratory culture media has long been noticed to cause distinct disadvantages in the cultural studies of the types responsible for the reddening of salt fish. Thus, routine analyses of salt and salt fish samples for the presence of red bacteria and also disinfectant studies leading to the elimination of the resulting contamination are seriously delayed. Lochhead's skim milk-salt agar medium has been modified by the addition of MgSO4∙7H2O, 5 g.; Mg(NO3)2∙6H2O, 1 g.; FeCl3∙7H2O, 0.025 g.; Proteose Peptone No. 3, 5 g.; and glycerol C.P., 10 g. to 1000 ml. of medium. In addition/to technical improvements, definite stimulation of growth has been obtained; the incubation time being cut in half and the volume of growth several times increased.

scholarly journals Phylogenetic Diversity of Bacteria Associated with the Marine Sponge Rhopaloeides odorabile

2001 ◽  
Vol 67 (1) ◽  
pp. 434-444 ◽  
Author(s):  
Nicole S. Webster ◽  
Kate J. Wilson ◽  
Linda L. Blackall ◽  
Russell T. Hill
Keyword(s):  
16S Rrna ◽  
Microbial Diversity ◽  
Marine Sponge ◽  
Culture Media ◽  
Laboratory Culture ◽  
Good Evidence ◽  
Molecular Techniques ◽  
Sponge Tissue ◽  
16S Rrna Analysis ◽  
Phylogenetic Affiliation

ABSTRACT Molecular techniques were employed to document the microbial diversity associated with the marine sponge Rhopaloeides odorabile. The phylogenetic affiliation of sponge-associated bacteria was assessed by 16S rRNA sequencing of cloned DNA fragments. Fluorescence in situ hybridization (FISH) was used to confirm the presence of the predominant groups indicated by 16S rDNA analysis. The community structure was extremely diverse with representatives of theActinobacteria, low-G+C gram-positive bacteria, the β- and γ-subdivisions of the Proteobacteria,Cytophaga/Flavobacterium, green sulfur bacteria, green nonsulfur bacteria, planctomycetes, and other sequence types with no known close relatives. FISH probes revealed the spatial location of these bacteria within the sponge tissue, in some cases suggesting possible symbiotic functions. The high proportion of 16S rRNA sequences derived from novel actinomycetes is good evidence for the presence of an indigenous marine actinomycete assemblage in R. odorabile. High microbial diversity was inferred from low duplication of clones in a library with 70 representatives. Determining the phylogenetic affiliation of sponge-associated microorganisms by 16S rRNA analysis facilitated the rational selection of culture media and isolation conditions to target specific groups of well-represented bacteria for laboratory culture. Novel media incorporating sponge extracts were used to isolate bacteria not previously recovered from this sponge.

scholarly journals Laboratory Culture Media-Dependent Biocontrol Ability of Burkholderia gladioli strain B543

2007 ◽  
Vol 23 (3) ◽  
pp. 161-165 ◽  
Author(s):  
Yeoung-Seuk Bae ◽  
Kyung-Seok Park ◽  
Ok-Hee Choi
Keyword(s):  
Culture Media ◽  
Laboratory Culture ◽  
Burkholderia Gladioli
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