scholarly journals Biological Effects of Tricalcium Silicate Nanoparticle-Containing Cement on Stem Cells from Human Exfoliated Deciduous Teeth

Nanomaterials ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1373
Author(s):  
Yoonsun Jung ◽  
Ji-Young Yoon ◽  
Kapil Dev Patel ◽  
Lan Ma ◽  
Hae-Hyoung Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Tissue Regeneration ◽  
Biological Effects ◽  
Physicochemical Analysis ◽  
Tricalcium Silicate ◽  
Deciduous Teeth ◽  
Dental Tissue ◽  
Odontogenic Differentiation ◽  
Starting Conditions ◽  
Human Exfoliated Deciduous Teeth

Nanomaterials can enhance interactions with stem cells for tissue regeneration. This study aimed to investigate the biological effects of tricalcium silicate nanoparticle-containing cement (Biodentine™) during or after setting on stem cells from human exfoliated deciduous teeth (SHED) to mimic clinically relevant situations in which materials are adapted. Specimens were divided into four groups depending on the start of extraction time (during (3, 6 and 12 min) or after setting (24 h)) and extracted in culture medium for 24 h for further physicochemical and biological analysis. After cell viability in serially diluted extracts was evaluated, odontogenic differentiation on SHED was evaluated by ARS staining using nontoxic conditions. A physicochemical analysis of extracts or specimens indicated different Ca ion content, pH, and surface chemistry among groups, supporting the possibility of different biological functionalities depending on the extraction starting conditions. Compared to the ‘after setting’ group, all ‘during setting’ groups showed cytotoxicity on SHED. The during setting groups induced more odontogenic differentiation at the nontoxic concentrations compared to the control. Thus, under clinically simulated extract conditions at nontoxic concentrations, Biodentine™ seemed to be a promising odontoblast differentiating biomaterial that is helpful for dental tissue regeneration. In addition, to simulate clinical situations when nanoparticle-containing cement is adjusted, biological effects during setting need to be considered.

Biological effects of low-level laser irradiation (LLLI) on stem cells from human exfoliated deciduous teeth (SHED)

2019 ◽  
Vol 24 (1) ◽  
pp. 167-180 ◽  
Author(s):  
M. Paschalidou ◽  
E. Athanasiadou ◽  
K. Arapostathis ◽  
N. Kotsanos ◽  
P. T. Koidis ◽  
...  
Keyword(s):  
Stem Cells ◽  
Laser Irradiation ◽  
Biological Effects ◽  
Deciduous Teeth ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Low Level Laser Irradiation ◽  
Human Exfoliated Deciduous Teeth

scholarly journals Comparative Analysis of Proliferation and Differentiation Potentials of Stem Cells from Inflamed Pulp of Deciduous Teeth and Stem Cells from Exfoliated Deciduous Teeth

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Shi Yu ◽  
Shu Diao ◽  
Jinsong Wang ◽  
Gang Ding ◽  
Dongmei Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Tissue Regeneration ◽  
Deciduous Teeth ◽  
Inflammatory Factors ◽  
Dental Tissue ◽  
Significant Difference ◽  
Proliferation And Differentiation ◽  
Cell Sources ◽  
Irreversible Pulpitis

Stem cells isolated from exfoliated deciduous teeth (SHEDs) are highly capable of proliferation and differentiation, and they represent good cell sources for mesenchymal stem cell- (MSC-) mediated dental tissue regeneration, but the supply of SHEDs is limited. A previous study found that stem cells could be isolated from inflamed tissues, but it is unknown whether primary dental pulp diagnosed with irreversible pulpitis might contain stem cells with appropriate tissue regeneration capacity. In this study, we aimed to isolate stem cells from both inflamed pulps of deciduous teeth (SCIDs) and SHEDs from Chinese children and to compare their proliferation and differentiation potentials. Our results showed that SCIDs were positive for cell surface markers, including CD105, CD90, and CD146, and they had high proliferation ability and osteogenic, adipogenic, and chondrogenic differentiation potentials. There was no significant difference in proliferation and differentiation potentials between SCIDs and SHEDs. The mRNA of inflammatory factors, including IL-1β, IL-6, and TNF-α, was expressed at similar levels in SCIDs and SHEDs, but SCIDs secreted more TNF-αprotein. In conclusion, ourin vitroresults showed that SCIDs have proliferation and differentiation potentials similar to those of SHEDs. Thus, SCIDs represent a new potentially applicable source for MSC mediated tissue regeneration.

Biological effects of silk fibroin 3D scaffolds on stem cells from human exfoliated deciduous teeth (SHEDs)

Odontology ◽  
2017 ◽  
Vol 106 (2) ◽  
pp. 125-134 ◽  
Author(s):  
M. Collado-González ◽  
M. P. Pecci-Lloret ◽  
D. García-Bernal ◽  
S. Aznar-Cervantes ◽  
R. E. Oñate-Sánchez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Silk Fibroin ◽  
Biological Effects ◽  
Deciduous Teeth ◽  
3D Scaffolds ◽  
Human Exfoliated Deciduous Teeth

Comparison of Isolation, Expansion and Cryopreservation Techniques to Produce Stem Cells from Human Exfoliated Deciduous teeth (SHED) with better Regenerative Potential

2020 ◽  
Vol 15 ◽  
Author(s):  
Sau Har Lee ◽  
Chung Yeng Looi ◽  
Pei Pei Chong ◽  
Jhi Biau Foo ◽  
Qi Hao Looi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adult Stem Cells ◽  
Ethical Issues ◽  
Deciduous Teeth ◽  
Dental Tissue ◽  
Stem Cells Therapy ◽  
Multiple Cell ◽  
Cryopreservation Techniques ◽  
Human Exfoliated Deciduous Teeth

: Mesenchymal stem cells (MSCs) are adult stem cells that are gaining worldwide attention for its multi-potential use in tissue engineering-based regenerative medicine. They can be obtained from numerous sources and one of the excellent source is from the dental tissue, such as stem cells that are extracted from the human exfoliated deciduous teeth (SHEDs). SHEDs are considered ideal due to its inherent characteristics, including the capability to proliferate quickly with minimal oncogenesis risk, multipotency capacity and its ability to suppress the immune system. On top of these positive cell traits, SHEDs are easily accessible with patient’s safety assured, posing less ethical issues and could also provide sufficient cells number for prospective clinical uses. This is primarily attributed to its ability to differentiate into multiple cell linages, including osteoblasts, odontoblasts, neuronal cells, adipocytes, as well as endothelial cells. Albeit SHEDs having a bright future, there still remains an obstacle to develop reliable experimental techniques to retain the long term regeneration potential of the stem cells for prospective research and clinical applications. Therefore, this review aims to describe the various isolation, expansion and cryopreservation techniques used by researchers in this stem cell field. Optimization of these techniques is crucial to obtain distinct SHEDs culture with preserved stem cells properties, which enable more reproducible results that will be the key for further stem cells therapy development.

Stem Cells Derived from Human Exfoliated Deciduous teeth [shed] in Neuronal Disorders: A Review

2020 ◽  
Vol 16 ◽  
Author(s):  
Minu Anoop ◽  
Indrani Datta
Keyword(s):  
Stem Cells ◽  
Neurodegenerative Diseases ◽  
Dental Pulp ◽  
Therapeutic Potential ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Proliferative Potential ◽  
Pulp Tissue ◽  
Human Exfoliated Deciduous Teeth

: Most conventional treatments for neurodegenerative diseases fail due to their focus on neuroprotection rather than neurorestoration. Stem cell‐based therapies are becoming a potential treatment option for neurodegenerative diseases as they can home in, engraft, differentiate and produce factors for CNS recovery. Stem cells derived from human dental pulp tissue differ from other sources of mesenchymal stem cells due to their embryonic neural crest origin and neurotrophic property. These include both dental pulp stem cells [DPSCs] from dental pulp tissues of human permanent teeth and stem cells from human exfoliated deciduous teeth [SHED]. SHED offer many advantages over other types of MSCs such as good proliferative potential, minimal invasive procurement, neuronal differentiation and neurotrophic capacity, and negligible ethical concerns. The therapeutic potential of SHED is attributed to the paracrine action of extracellularly released secreted factors, specifically the secretome, of which exosomes is a key component. SHED and its conditioned media can be effective in neurodegeneration through multiple mechanisms, including cell replacement, paracrine effects, angiogenesis, synaptogenesis, immunomodulation, and apoptosis inhibition, and SHED exosomes offer an ideal refined bed-to-bench formulation in neurodegenerative disorders. However, in spite of these advantages, there are still some limitations of SHED exosome therapy, such as the effectiveness of long-term storage of SHED and their exosomes, the development of a robust GMP-grade manufacturing protocol, optimization of the route of administration, and evaluation of the efficacy and safety in humans. In this review, we have addressed the isolation, collection and properties of SHED along with its therapeutic potential on in vitro and in vivo neuronal disorder models as evident from the published literature.

In vitro and in vivo characteristics of stem cells from human exfoliated deciduous teeth obtained by enzymatic disaggregation and outgrowth

2014 ◽  
Vol 59 (10) ◽  
pp. 1013-1023 ◽  
Author(s):  
Mijeong Jeon ◽  
Je Seon Song ◽  
Byung-Jai Choi ◽  
Hyung-Jun Choi ◽  
Dong-Min Shin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Deciduous Teeth ◽  
Human Exfoliated Deciduous Teeth
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