scholarly journals Retaining Skin Barrier Function Properties of the Stratum Corneum with Components of the Natural Moisturizing Factor—A Randomized, Placebo-Controlled Double-Blind In Vivo Study

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1649
Author(s):  
Johannes Schleusener ◽  
Andrew Salazar ◽  
Jörg von Hagen ◽  
Jürgen Lademann ◽  
Maxim E. Darvin
Keyword(s):  
Stratum Corneum ◽  
Barrier Function ◽  
Bound Water ◽  
Skin Barrier ◽  
Skin Barrier Function ◽  
Double Blind Study ◽  
Weakly Bound ◽  
Double Blind

The influence of a topically applied formulation containing components of natural moisturizing factor (NMF) on barrier-related parameters of the stratum corneum (SC) was investigated in vivo using confocal Raman microspectroscopy in a randomized, placebo-controlled double-blind study on 12 volunteers for 14 days. This method allowed for the elucidation of subtle differences between the verum and the placebo even though the components of the verum naturally occur in the SC. This differentiation is not possible non-invasively by conventional methods. In this study, we found that the applied verum and placebo formulations disrupted the equilibrium of water, NMF and lipids in the SC. The adverse effects of the formulation could be mitigated by incorporating it into a simplified supplementation of NMF molecules. As a long-term effect, the amount of strongly bound water increases at 30–40% SC depth (p < 0.05) and the amount of weakly bound water decreases at 30–40% SC depth (p < 0.05) for the verum. This supplement was also unexpectedly able to prevent intercellular lipids (ICL) disorganization in selected depths. In the long term, the verum treatment limited the lateral disorganization of the ICL to the upper 20% SC depth. Further research is required to elucidate the interplay of these factors in the SC, to better understand their contribution to the equilibrium and barrier function of the skin. This understanding of the interaction of these naturally occurring components could help in the future to develop and optimize topical treatments for diseases like psoriasis, atopic dermatitis, ichthyosis where the skin barrier is disrupted.

scholarly journals Clinical Evaluation of Topical Lotion Containing 2-Aza-8-Oxohypoxanthine on Skin Barrier Function against Water Loss

Cosmetics ◽  
2021 ◽  
Vol 8 (3) ◽  
pp. 83
Author(s):  
Hisae Aoshima ◽  
Rinta Ibuki ◽  
Masayuki Ito ◽  
Hirokazu Kawagishi
Keyword(s):  
Moisture Content ◽  
Stratum Corneum ◽  
Water Loss ◽  
Barrier Function ◽  
Statistical Difference ◽  
Skin Barrier ◽  
Comparative Trial ◽  
Skin Barrier Function ◽  
Double Blind ◽  
Split Face

A clinical study was conducted to evaluate the efficacy of 2-aza-8-oxohypoxanthine (AOH) on human skin in a double-blind, placebo-controlled, and split-face comparative trial. To this end, a topical lotion containing 0.1% of AOH and its placebo formulation were applied in 20 Japanese subjects (age: 41–58 years, average: 48.4 ± 4.7 years) to their faces daily for eight weeks. The moisture content in the stratum corneum and the trans-epidermal water loss (TEWL) were measured at the beginning of the study and after eight weeks. The results obtained upon comparing the data of the two measuring points showed that the 0.1% AOH lotion caused a statistically significant larger decrease in TEWL after eight weeks. Moreover, the 0.1% AOH lotion produced a larger increase in the moisture content in the stratum corneum after eight weeks, although there was no statistical difference between the two formulations. These results clearly indicate that AOH is effective as a cosmetic agent with a skin barrier function against water loss.

A depth-dependent profile of the lipid conformation and lateral packing order of the stratum corneum in vivo measured using Raman microscopy

The Analyst ◽  
2016 ◽  
Vol 141 (6) ◽  
pp. 1981-1987 ◽  
Author(s):  
ChunSik Choe ◽  
Jürgen Lademann ◽  
Maxim E. Darvin
Keyword(s):  
Stratum Corneum ◽  
Barrier Function ◽  
Skin Barrier ◽  
Raman Microscopy ◽  
Skin Barrier Function ◽  
Lipid Structure ◽  
Lipid Conformation ◽  
Intercellular Lipid

The intercellular lipid structure of the stratum corneum (SC) plays a key role in skin barrier function.

Effect of orally administered collagen hydrolysate on gene expression profiles in mouse skin: a DNA microarray analysis

2015 ◽  
Vol 47 (8) ◽  
pp. 355-363 ◽  
Author(s):  
Chisato Oba ◽  
Kyoko Ito ◽  
Satomi Ichikawa ◽  
Masashi Morifuji ◽  
Yuji Nakai ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stratum Corneum ◽  
Water Content ◽  
Barrier Function ◽  
Skin Barrier ◽  
Skin Barrier Function ◽  
Skin Elasticity ◽  
Collagen Hydrolysate ◽  
Stratum Corneum Water Content

Dietary collagen hydrolysate has been hypothesized to improve skin barrier function. To investigate the effect of long-term collagen hydrolysate administration on the skin, we evaluated stratum corneum water content and skin elasticity in intrinsically aged mice. Female hairless mice were fed a control diet or a collagen hydrolysate-containing diet for 12 wk. Stratum corneum water content and skin elasticity were gradually decreased in chronologically aged control mice. Intake of collagen hydrolysate significantly suppressed such changes. Moreover, we used DNA microarrays to analyze gene expression in the skin of mice that had been administered collagen hydrolysate. Twelve weeks after the start of collagen intake, no significant differences appeared in the gene expression profile compared with the control group. However, 1 wk after administration, 135 genes were upregulated and 448 genes were downregulated in the collagen group. This suggests that gene changes preceded changes of barrier function and elasticity. We focused on several genes correlated with functional changes in the skin. Gene Ontology terms related to epidermal cell development were significantly enriched in upregulated genes. These skin function-related genes had properties that facilitate epidermal production and differentiation while suppressing dermal degradation. In conclusion, our results suggest that altered gene expression at the early stages after collagen administration affects skin barrier function and mechanical properties. Long-term oral intake of collagen hydrolysate improves skin dysfunction by regulating genes related to production and maintenance of skin tissue.

Topical Formulation Containing Beeswax-Based Nanoparticles Improved In Vivo Skin Barrier Function

2017 ◽  
Vol 18 (7) ◽  
pp. 2505-2516 ◽  
Author(s):  
Carla Souza ◽  
Luis Alexandre Pedro de Freitas ◽  
Patrícia Maria Berardo Gonçalves Maia Campos
Keyword(s):  
Barrier Function ◽  
Skin Barrier ◽  
Skin Barrier Function ◽  
Topical Formulation

scholarly journals Immediate and long-term effects of polysaccharides-based formulations on human skin

2012 ◽  
Vol 48 (3) ◽  
pp. 547-555 ◽  
Author(s):  
Flavio Bueno de Camargo Junior ◽  
Lorena Rigo Gaspar ◽  
Patrícia Maria Berardo Gonçalves Maia Campos
Keyword(s):  
Barrier Function ◽  
Skin Barrier ◽  
Skin Hydration ◽  
Skin Barrier Function ◽  
Cell Renewal ◽  
Myrtus Communis ◽  
Long Term Effects ◽  
Subjective Analysis ◽  
Cosmetic Formulations

A new trend in cosmetic formulations is the use of biotechnological raw materials as the polysaccharides from Klebsiella pneumoniae, which are supposed to enhance cell renewal, improve skin hydration and micro-relief. Botanical extracts of Myrtus communis leaves contain different sugars, which may provide the same benefits. Thus, the objective of this study was to evaluate through objective and subjective analysis the immediate and long-term effects of cosmetic formulations containing polysaccharides biotechnologically-originated and / or the ones contained in Myrtus communis extracts. Three polysaccharide-based and placebo formulations were applied on the forearm skin of 40 volunteers. Skin hydration, transepidermal water loss (TEWL), viscoelasticity and skin micro-relief measurements were made before and 2 hours after a single application and after 15 and 30 day-periods of daily applications. Answers to a questionnaire about perceptions of formulation cosmetic features constituted the subjective analysis. All polysaccharide-based formulations enhanced skin hydration. Formulations with isolated or combined active substances improved skin barrier function as compared to placebo, in the short and long term studies. Formulations containing Myrtus communis extracts had the highest acceptance. Results suggest that daily use of formulations containing these substances is important for protection of the skin barrier function.

scholarly journals Effects of Cast Immobilisation on Skin Barrier Function

2020 ◽  
Vol 49 (6) ◽  
pp. 354-359
Author(s):  
Chin Yee Woo ◽  
Mark JA Koh ◽  
Winnie KY Fung ◽  
Cheri SH Chan ◽  
Chong Bing Chua ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Water Loss ◽  
Barrier Function ◽  
Skin Irritation ◽  
Skin Barrier ◽  
Transepidermal Water Loss ◽  
Skin Barrier Function ◽  
Hair Density ◽  
Cast Immobilisation ◽  
Volar Surface

Introduction: Cast immobilisation remains the mainstay of treatment for various fractures in paediatric patients, yet patients commonly complain of skin irritation and discomfort. This study aimed to perform a qualitative and quantitative evaluation of the effects of cast immobilisation on the skin of children and adolescents. Materials and Methods: Patients aged 6–17 years of age with a fracture treated in a fiberglass short-arm or short-leg cast were recruited. Transepidermal water loss (TEWL), stratum corneum (SC) hydration, hair density and presence of any skin signs were assessed before and after cast. Patients were required to complete a weekly questionnaire to rate itch, malodour, warmth, and dampness of the skin under the cast. Results: A total of 60 subjects completed the study. Thirty-six patients received a short-arm cast; 24 received a short-leg cast. Upon cast removal, TEWL was significantly increased on the volar surface of the arms and legs (P <0.05), and the dorsal surface of the arm (P <0.05). Likewise, SC hydration was significantly increased at most sites (P <0.05), except the volar surface of the leg (P = 0.513). There was no change in hair density. Throughout the duration of casting, there was an increase in itch and malodour scores. Conclusions: Moderate but significant changes in TEWL, SC hydration and subjective symptoms were observed during the duration of cast immobilisation, demonstrating that cast immobilisation for up to 4 weeks exerts moderate adverse impact on patients’ skin. Further studies to explore the use of better materials for cast immobilisation to improve skin barrier function and overall patient satisfaction are warranted. Ann Acad Med Singapore 2020;49:285–93 Ann Acad Med Singapore 2020;49:354–59 Key words: Cast immobilisation, Transepidermal water loss, Stratum corneum hydration

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