scholarly journals Phytic Acid Protects from Oxidative Stress Induced by Iron-Overload and High-Fat Diets in ß2-Microglobulin Knockout Mice

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5331
Author(s):  
Sixtus Aguree ◽  
Ling Guo ◽  
Manju B. Reddy
Keyword(s):  
Oxidative Stress ◽  
Iron Overload ◽  
Phytic Acid ◽  
Iron Concentration ◽  
Basal Diet ◽  
Nonheme Iron ◽  
High Fat ◽  
Free Iron ◽  
High Fat Diets ◽  
Fat Diets

The objective of this study was to examine the protective effect of phytic acid (PA) in reducing oxidative stress in an animal model for human hereditary hemochromatosis (HH) fed high-fat diets. Sixty-four ß2 microglobulin knockout (β2m KO) mice were randomly assigned to three treatments by feeding: control (basal), atherogenic (AT), and polyunsaturated fatty acid (PUFA) diets. One-half of the mice in each treatment group were fed 2% (wt/wt) PA. The ß2m+/+ mice (wild type (WT)) were fed a basal diet. All seven groups were fed for 10 weeks with a 50-ppm iron-containing diet (AIN-93G). Free iron and lipids were measured in serum samples. Nonheme iron, thiobarbituric acid-reactive substances (TBARS), superoxide dismutase (SOD), and catalase concentrations were measured in the liver tissue. Nonheme iron concentration in ß2m KO mice (on the basal diet) was 20× higher (p < 0.0001) than in the WT mice. Compared to the WT mice, ß2m KO mice had a significantly higher concentration of free iron in the serum (p < 0.0001), six-fold higher hepatic TBARs (p < 0.0001), and 18% lower hepatic SOD level. When PA was added to the β2m KO basal diet, a reduction (26 to 50%) of iron concentration was seen in the liver and heart. The addition of PA also significantly reduced TBARs in all three dietary groups of the iron-overloaded group, but most effectively in the control group. An increase in SOD concentration was seen only in the PUFA group, but serum triacylglycerol (TG) concentration was reduced in both dietary fat groups. In conclusion, our results suggest that PA protects against oxidative stress-induced by genetic iron overload alone or when fed high fat.

Resveratrol toxicity: Effects on risk factors for atherosclerosis and hepatic oxidative stress in standard and high-fat diets

2009 ◽  
Vol 47 (6) ◽  
pp. 1362-1367 ◽  
Author(s):  
K.K.R. Rocha ◽  
G.A. Souza ◽  
G.X. Ebaid ◽  
F.R.F. Seiva ◽  
A.C. Cataneo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Risk Factors ◽  
High Fat ◽  
Hepatic Oxidative Stress ◽  
High Fat Diets ◽  
Fat Diets ◽  
Toxicity Effects

The combination of maternal and offspring high-fat diets causes marked oxidative stress and development of metabolic syndrome in mouse offspring

Life Sciences ◽  
2016 ◽  
Vol 151 ◽  
pp. 70-75 ◽  
Author(s):  
Junya Ito ◽  
Kiyotaka Nakagawa ◽  
Shunji Kato ◽  
Taiki Miyazawa ◽  
Fumiko Kimura ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Metabolic Syndrome ◽  
High Fat ◽  
High Fat Diets ◽  
Fat Diets

FURTHER STUDIES ON LIPOTROPISM IN THE DOMESTIC DUCK (White Pekin)

1953 ◽  
Vol 31 (6) ◽  
pp. 474-484
Author(s):  
Denyse D. Duchesne ◽  
Richard Bernard
Keyword(s):  
Soybean Protein ◽  
Basal Diet ◽  
Total Lipids ◽  
High Fat ◽  
Liver Lipids ◽  
High Fat Diets ◽  
Free Choline ◽  
Fatty Livers ◽  
Fat Diets ◽  
Glyceride Fraction

On choline-free diets containing 10, 18, and 28% casein, the total liver lipids of ducklings were found to be 34.8, 25.0, and 13.8% respectively. Supplementation of the 10% casein diet with 0.5% methionine increased the liver lipids. The same amino acid has no effect at the intermediate level of casein while it is lipotropic at the 28% casein level. In similar experiments with a purified soybean protein (low in methionine) fed at levels of 12, 21.7, and 27.9%, the liver lipids were 31.8, 26.6, and 27.6% respectively. These values do not differ significantly from each other. Methionine was found to be lipotropic at any of these three levels of soybean protein, and more so as the level of the soybean protein increased in the diet. From these results, it is concluded that the purified soybean protein used is a nonlipotropic protein, while casein is definitely lipotropic in ducklings. The lipotropic activity of methionine is closely related to the quality and quantity of the protein in the diet. On high fat diets containing 22, 32, and 42% fat, choline had no lipotropic effect. The average liver lipids for all groups ranged from 11 to 15%. High fat diets are less inducive of fatty livers than high carbohydrate diets. Ducklings fed a fat-free, choline-free basal diet presented livers with an average total lipids of 18%. Supplementing the basal diet with 1% cholesterol increased the liver lipids to 22%. Inositol is lipotropic either in the presence or absence of cholesterol, but choline was much more effective under similar conditions. The two lipotropic factors have a synergistic action and the reduction of the liver lipids is primarily on the glyceride fraction. Cholesteryl ester was at its lowest value in the group fed the basal diet supplemented with inositol only.

Changing the paradigm for myoglobin: a novel link between lipids and myoglobin

2014 ◽  
Vol 117 (3) ◽  
pp. 307-315 ◽  
Author(s):  
Amber E. Schlater ◽  
Michael A. De Miranda ◽  
Melinda A. Frye ◽  
Stephen J. Trumble ◽  
Shane B. Kanatous
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Tissue Sample ◽  
Oxidative Stress Marker ◽  
Oxygen Binding ◽  
High Fat ◽  
Western Blots ◽  
High Fat Diets ◽  
Lipid Supplementation ◽  
Fat Diets

Myoglobin (Mb) is an oxygen-binding muscular hemeprotein regulated via Ca2+-signaling pathways involving calcineurin (CN), with Mb increases attributed to hypoxia, exercise, and nitric oxide. Here, we show a link between lipid supplementation and increased Mb in skeletal muscle. C2C12 cells were cultured in normoxia or hypoxia with glucose or 5% lipid. Mb assays revealed that lipid cohorts had higher Mb than control cohorts in both normoxia and hypoxia, whereas Mb Western blots showed lipid cohorts having higher Mb than control cohorts exclusively under hypoxia. Normoxic cells were compared with soleus tissue from normoxic rats fed high-fat diets; whereas tissue sample cohorts showed no difference in CO-binding Mb, fat-fed rats showed increases in total Mb protein (similar to hypoxic cells), suggesting increases in modified Mb. Moreover, Mb increases did not parallel CN increases but did, however, parallel oxidative stress marker augmentation. Addition of antioxidant prevented Mb increases in lipid-supplemented normoxic cells and mitigated Mb increases in lipid-supplemented hypoxic cells, suggesting a pathway for Mb regulation through redox signaling independent of CN.

The interconnection of high-fat diets, oxidative stress, the heart, and carcinogenesis

Cancer ◽  
2021 ◽  
pp. 111-120
Author(s):  
Bianka Bojková ◽  
Natalia Kurhaluk ◽  
Pawel J. Winklewski
Keyword(s):  
Oxidative Stress ◽  
High Fat ◽  
High Fat Diets ◽  
Fat Diets

scholarly journals The Combination of Mulberry Extracts and Silk Amino Acids Alleviated High Fat Diet-Induced Nonalcoholic Hepatic Steatosis by Improving Hepatic Insulin Signaling and Normalizing Gut Microbiome Dysbiosis in Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-17 ◽  
Author(s):  
Sunmin Park ◽  
Ting Zhang ◽  
Jing Yi Qiu ◽  
Xuangao Wu
Keyword(s):  
Oxidative Stress ◽  
Amino Acids ◽  
Disease Control ◽  
Normal Control ◽  
Insulin Signaling ◽  
High Fat ◽  
Hepatic Insulin Signaling ◽  
High Fat Diets ◽  
Fat Diets ◽  
Better Than

Mulberry water extracts (MB) and silk amino acids (SA) are reported to improve oxidative stress and inflammation, respectively. We hypothesized whether the mixture of mulberry water extracts and silk amino acids can alleviate nonalcoholic fatty liver disease (NAFLD) induced by high fat diets. Male Sprague Dawley rats were orally provided with high fat diets containing different ratios of MB and SA (1:3, MS1:3, or 1:5, MS1:5) or cellulose (the disease-control) for 12 weeks. Rats had 200 or 600 mg/kg bw of MS1:3 and MS1:5 (MS1:3-L, MS1:3-H; MS1:5-L, and MS1:5-H). Rats in the normal-control group were fed the 20% fat diet with cellulose. Disease-control rats exhibited much greater triglyceride (TG) deposition in the liver than the normal-control rats along with increased body weight gain, visceral fat mass, serum concentrations of cholesterol, triglyceride and nonesterified fatty acid (NEFA), and insulin resistance. Disease-control rats also had liver damage with increased oxidative stress and inflammation compared to the normal-control rats. MS1:3-H and MS1:5-H were found to have greater hepatic glycogen accumulation and decreased hepatic TG, insulin resistance, and dyslipidemia, with MS1:5-H being similar to the normal-control. MS1:3-H alleviated oxidative stress with lower hepatic lipid peroxide compared to MS1:5-H whereas MS1:5-H ameliorated inflammation and hepatocyte damage better than MS1:3-H. Both MS1:3-H and MS1:5-H potentiated hepatic insulin signaling (pAkt⟶pACC) and reduced the mRNA expression of TG synthesis genes mRNA (FAS and SREBP-1c). In the gut microbiome MS1:3-H elevated the ratio of Bacteroidales to Clostridiales in the cecum better than MS1:5-H but MS1:5-H reduced the proinflammatory Turicibacterales. In conclusion, both MS1:3-H and MS1:5-H prevented liver damage induced by high fat diets, mainly by suppressing oxidative stress and inflammation, respectively. MS1:3 and MS1:5 might be used as therapeutic agent for NAFLD.

scholarly journals Oxidative stress and fatty acid profile in Wistar rats subjected to acute food restriction and refeeding with high-fat diets

2014 ◽  
Vol 29 (3) ◽  
pp. 178-185 ◽  
Author(s):  
Ana Lígia da Silva Nassar ◽  
Luisa Pereira Marot ◽  
Paula Payão Ovidio ◽  
Gabriela Salim Ferreira de Castro ◽  
Alceu Afonso Jordão Júnior
Keyword(s):  
Oxidative Stress ◽  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Food Restriction ◽  
Wistar Rats ◽  
High Fat ◽  
High Fat Diets ◽  
Fat Diets

Effect of Chitosan on Hepatic Drug-Metabolizing Enzymes and Oxidative Stress in Rats Fed Low- and High-Fat Diets

2010 ◽  
Vol 58 (8) ◽  
pp. 5187-5193 ◽  
Author(s):  
Hsien-Tsung Yao ◽  
Chong-Kuei Lii ◽  
Ruey-Hwang Chou ◽  
Jia-Hsuan Lin ◽  
Hui-Ting Yang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Drug Metabolizing Enzymes ◽  
High Fat ◽  
Metabolizing Enzymes ◽  
Hepatic Drug ◽  
High Fat Diets ◽  
Fat Diets ◽  
And Oxidative Stress
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