scholarly journals The Combination of Mulberry Extracts and Silk Amino Acids Alleviated High Fat Diet-Induced Nonalcoholic Hepatic Steatosis by Improving Hepatic Insulin Signaling and Normalizing Gut Microbiome Dysbiosis in Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-17 ◽  
Author(s):  
Sunmin Park ◽  
Ting Zhang ◽  
Jing Yi Qiu ◽  
Xuangao Wu
Keyword(s):  
Oxidative Stress ◽  
Amino Acids ◽  
Disease Control ◽  
Normal Control ◽  
Insulin Signaling ◽  
High Fat ◽  
Hepatic Insulin Signaling ◽  
High Fat Diets ◽  
Fat Diets ◽  
Better Than

Mulberry water extracts (MB) and silk amino acids (SA) are reported to improve oxidative stress and inflammation, respectively. We hypothesized whether the mixture of mulberry water extracts and silk amino acids can alleviate nonalcoholic fatty liver disease (NAFLD) induced by high fat diets. Male Sprague Dawley rats were orally provided with high fat diets containing different ratios of MB and SA (1:3, MS1:3, or 1:5, MS1:5) or cellulose (the disease-control) for 12 weeks. Rats had 200 or 600 mg/kg bw of MS1:3 and MS1:5 (MS1:3-L, MS1:3-H; MS1:5-L, and MS1:5-H). Rats in the normal-control group were fed the 20% fat diet with cellulose. Disease-control rats exhibited much greater triglyceride (TG) deposition in the liver than the normal-control rats along with increased body weight gain, visceral fat mass, serum concentrations of cholesterol, triglyceride and nonesterified fatty acid (NEFA), and insulin resistance. Disease-control rats also had liver damage with increased oxidative stress and inflammation compared to the normal-control rats. MS1:3-H and MS1:5-H were found to have greater hepatic glycogen accumulation and decreased hepatic TG, insulin resistance, and dyslipidemia, with MS1:5-H being similar to the normal-control. MS1:3-H alleviated oxidative stress with lower hepatic lipid peroxide compared to MS1:5-H whereas MS1:5-H ameliorated inflammation and hepatocyte damage better than MS1:3-H. Both MS1:3-H and MS1:5-H potentiated hepatic insulin signaling (pAkt⟶pACC) and reduced the mRNA expression of TG synthesis genes mRNA (FAS and SREBP-1c). In the gut microbiome MS1:3-H elevated the ratio of Bacteroidales to Clostridiales in the cecum better than MS1:5-H but MS1:5-H reduced the proinflammatory Turicibacterales. In conclusion, both MS1:3-H and MS1:5-H prevented liver damage induced by high fat diets, mainly by suppressing oxidative stress and inflammation, respectively. MS1:3 and MS1:5 might be used as therapeutic agent for NAFLD.

scholarly journals Dietary phosphatidylcholine promotes breast cancer in the hyperlipidemic E0771 murine model

2020 ◽  
Author(s):  
Fredrick O Onono ◽  
Lakshman Chelvarajan ◽  
Baoxiang Yan ◽  
Ebubechi Adindu ◽  
Esias Bedingar
Keyword(s):  
Breast Cancer ◽  
Mass Spectrometry ◽  
Amino Acids ◽  
Tumor Growth ◽  
Cancer Development ◽  
Primary Breast Tumor ◽  
High Fat ◽  
Breast Cancer Development ◽  
High Fat Diets ◽  
Fat Diets

Abstract BackgroundCancer cells are characterized by aberrant phosphatidylcholine (PC) metabolism. PC can be synthesized de novo or absorbed from diet, after digestion, by the intestinal enterocytes. Here, we investigated the association of dietary intake of PC and breast cancer development in mice. MethodsWe used tandem mass spectrometry methods to quantitate PC content of various fat sources used to manufacture rodent diets. Rodent diets were then formulated with either casein or amino acids in place of casein. To test the effects of dietary PC on tumor growth we fed low density lipoprotein receptor-null (LDLR–/–) mice high fat diets formulated with casein (high PC) or amino acids in place of casein (low PC). Endogenous PC biosynthesis and levels of total circulated plasma PC was monitored using stable isotope tracer choline and mass spectrometry analysis. Tumors were induced in mice after 12 weeks of high fat diet feeding. Since PC-derived molecules are important transducers of mitogenic signals, we tested the effects of inhibiting production of lysophosphatidic acid (LPA) using a recently described autotaxin (ATX) inhibitor. Finally, plasma inflammatory cytokine levels were analyzed to determine the effects of diets and ATX inhibition on systemic cytokine milieu. ResultsWe found that casein is the main source of PC when present in rodent diets. Replacing casein with amino acids increased the relative proportion of endogenously biosynthesized PC in mouse plasma. Compared to diets containing casein, amino acid-defined diets decreased primary tumor growth in the hyperlipidemic estrogen-receptor positive E0771 breast cancer mouse model. Inhibition of autotaxin with the potent inhibitor PAT-505 did not attenuate breast cancer development in these hyperlipidemic mice. Further, replacing casein with amino acids or treatment with PAT-505 significantly reduced systemic markers of inflammation. ConclusionOur results show that casein is a significant source of PC when present in rodent diets. Diets formulated with amino acids in place of casein have higher proportion of circulating PC from the endogenous biosynthetic pool. Casein-containing high fat diets promote primary breast tumor development in mice through mechanisms that involve systemic inflammation but is independent of LPA production by autotaxin.

Resveratrol toxicity: Effects on risk factors for atherosclerosis and hepatic oxidative stress in standard and high-fat diets

2009 ◽  
Vol 47 (6) ◽  
pp. 1362-1367 ◽  
Author(s):  
K.K.R. Rocha ◽  
G.A. Souza ◽  
G.X. Ebaid ◽  
F.R.F. Seiva ◽  
A.C. Cataneo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Risk Factors ◽  
High Fat ◽  
Hepatic Oxidative Stress ◽  
High Fat Diets ◽  
Fat Diets ◽  
Toxicity Effects

The combination of maternal and offspring high-fat diets causes marked oxidative stress and development of metabolic syndrome in mouse offspring

Life Sciences ◽  
2016 ◽  
Vol 151 ◽  
pp. 70-75 ◽  
Author(s):  
Junya Ito ◽  
Kiyotaka Nakagawa ◽  
Shunji Kato ◽  
Taiki Miyazawa ◽  
Fumiko Kimura ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Metabolic Syndrome ◽  
High Fat ◽  
High Fat Diets ◽  
Fat Diets

Changing the paradigm for myoglobin: a novel link between lipids and myoglobin

2014 ◽  
Vol 117 (3) ◽  
pp. 307-315 ◽  
Author(s):  
Amber E. Schlater ◽  
Michael A. De Miranda ◽  
Melinda A. Frye ◽  
Stephen J. Trumble ◽  
Shane B. Kanatous
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Tissue Sample ◽  
Oxidative Stress Marker ◽  
Oxygen Binding ◽  
High Fat ◽  
Western Blots ◽  
High Fat Diets ◽  
Lipid Supplementation ◽  
Fat Diets

Myoglobin (Mb) is an oxygen-binding muscular hemeprotein regulated via Ca2+-signaling pathways involving calcineurin (CN), with Mb increases attributed to hypoxia, exercise, and nitric oxide. Here, we show a link between lipid supplementation and increased Mb in skeletal muscle. C2C12 cells were cultured in normoxia or hypoxia with glucose or 5% lipid. Mb assays revealed that lipid cohorts had higher Mb than control cohorts in both normoxia and hypoxia, whereas Mb Western blots showed lipid cohorts having higher Mb than control cohorts exclusively under hypoxia. Normoxic cells were compared with soleus tissue from normoxic rats fed high-fat diets; whereas tissue sample cohorts showed no difference in CO-binding Mb, fat-fed rats showed increases in total Mb protein (similar to hypoxic cells), suggesting increases in modified Mb. Moreover, Mb increases did not parallel CN increases but did, however, parallel oxidative stress marker augmentation. Addition of antioxidant prevented Mb increases in lipid-supplemented normoxic cells and mitigated Mb increases in lipid-supplemented hypoxic cells, suggesting a pathway for Mb regulation through redox signaling independent of CN.

The interconnection of high-fat diets, oxidative stress, the heart, and carcinogenesis

Cancer ◽  
2021 ◽  
pp. 111-120
Author(s):  
Bianka Bojková ◽  
Natalia Kurhaluk ◽  
Pawel J. Winklewski
Keyword(s):  
Oxidative Stress ◽  
High Fat ◽  
High Fat Diets ◽  
Fat Diets

scholarly journals Oxidative stress and fatty acid profile in Wistar rats subjected to acute food restriction and refeeding with high-fat diets

2014 ◽  
Vol 29 (3) ◽  
pp. 178-185 ◽  
Author(s):  
Ana Lígia da Silva Nassar ◽  
Luisa Pereira Marot ◽  
Paula Payão Ovidio ◽  
Gabriela Salim Ferreira de Castro ◽  
Alceu Afonso Jordão Júnior
Keyword(s):  
Oxidative Stress ◽  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Food Restriction ◽  
Wistar Rats ◽  
High Fat ◽  
High Fat Diets ◽  
Fat Diets

Effect of Chitosan on Hepatic Drug-Metabolizing Enzymes and Oxidative Stress in Rats Fed Low- and High-Fat Diets

2010 ◽  
Vol 58 (8) ◽  
pp. 5187-5193 ◽  
Author(s):  
Hsien-Tsung Yao ◽  
Chong-Kuei Lii ◽  
Ruey-Hwang Chou ◽  
Jia-Hsuan Lin ◽  
Hui-Ting Yang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Drug Metabolizing Enzymes ◽  
High Fat ◽  
Metabolizing Enzymes ◽  
Hepatic Drug ◽  
High Fat Diets ◽  
Fat Diets ◽  
And Oxidative Stress
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