scholarly journals Tooth-Supporting Hard Tissue Regeneration Using Biopolymeric Material Fabrication Strategies

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4802
Author(s):  
Min Guk Kim ◽  
Chan Ho Park
Keyword(s):  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Alveolar Bone ◽  
Critical Role ◽  
Periodontal Regeneration ◽  
Tissue Formation ◽  
Hard Tissue ◽  
Periodontal Tissues ◽  
Mineralized Tissues ◽  
Biomechanical Forces

The mineralized tissues (alveolar bone and cementum) are the major components of periodontal tissues and play a critical role to anchor periodontal ligament (PDL) to tooth-root surfaces. The integrated multiple tissues could generate biological or physiological responses to transmitted biomechanical forces by mastication or occlusion. However, due to periodontitis or traumatic injuries, affect destruction or progressive damage of periodontal hard tissues including PDL could be affected and consequently lead to tooth loss. Conventional tissue engineering approaches have been developed to regenerate or repair periodontium but, engineered periodontal tissue formation is still challenging because there are still limitations to control spatial compartmentalization for individual tissues and provide optimal 3D constructs for tooth-supporting tissue regeneration and maturation. Here, we present the recently developed strategies to induce osteogenesis and cementogenesis by the fabrication of 3D architectures or the chemical modifications of biopolymeric materials. These techniques in tooth-supporting hard tissue engineering are highly promising to promote the periodontal regeneration and advance the interfacial tissue formation for tissue integrations of PDL fibrous connective tissue bundles (alveolar bone-to-PDL or PDL-to-cementum) for functioning restorations of the periodontal complex.

Regenereation in periodontics

2018 ◽  
Author(s):  
Murtaza Kaderi ◽  
Mohsin Ali ◽  
Alfiya Ali ◽  
Tasneem Kaderi
Keyword(s):  
Tissue Engineering ◽  
Clinical Practice ◽  
Periodontal Disease ◽  
Disease Progression ◽  
Tissue Regeneration ◽  
Surgical Procedures ◽  
Periodontal Regeneration ◽  
Guided Tissue Regeneration ◽  
Periodontal Tissues ◽  
Extensive Documentation

The goals of periodontal therapy are to arrest of periodontal disease progression and to attain the regeneration of the periodontal apparatus. Osseous grafting and Guided tissue regeneration (GTR) are the two techniques with the most extensive documentation of periodontal regeneration. However, these techniques offer limited potential towards regenerating the periodontal tissues. Recent surgical procedures and application of newer materials aim at greater and more predictable regeneration with the concept of tissue engineering for enhanced periodontal regeneration and functional attachment have been developed, analyzed, and employed in clinical practice

scholarly journals Biomaterial-Based Approaches for Regeneration of Periodontal Ligament and Cementum Using 3D Platforms

2019 ◽  
Vol 20 (18) ◽  
pp. 4364 ◽  
Author(s):  
Chan Ho Park
Keyword(s):  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Periodontal Ligament ◽  
State Of The Art ◽  
Tooth Root ◽  
Tissue Formation ◽  
Periodontal Tissue ◽  
Periodontal Tissues ◽  
Root Surfaces ◽  
Periodontal Ligaments

Currently, various tissue engineering strategies have been developed for multiple tissue regeneration and integrative structure formations as well as single tissue formation in musculoskeletal complexes. In particular, the regeneration of periodontal tissues or tooth-supportive structures is still challenging to spatiotemporally compartmentalize PCL (poly-ε-caprolactone)-cementum constructs with micron-scaled interfaces, integrative tissue (or cementum) formations with optimal dimensions along the tooth-root surfaces, and specific orientations of engineered periodontal ligaments (PDLs). Here, we discuss current advanced approaches to spatiotemporally control PDL orientations with specific angulations and to regenerate cementum layers on the tooth-root surfaces with Sharpey’s fiber anchorages for state-of-the-art periodontal tissue engineering.

scholarly journals Application of a BMP2-binding heparan sulphate to promote periodontal regeneration

2021 ◽  
Vol 42 ◽  
pp. 139-153
Author(s):  
BQ Le ◽  
◽  
JH Too ◽  
TC Tan ◽  
RAA Smith ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Tissue Regeneration ◽  
Alveolar Bone ◽  
Heparan Sulphate ◽  
Periodontal Regeneration ◽  
Bone Morphogenetic Protein 2 ◽  
Bone Tissue Regeneration ◽  
Tissue Loss ◽  
Periodontal Tissues ◽  
Combination Device

Periodontitis is the most common inflammatory disease that leads to periodontal defects and tooth loss. Regeneration of alveolar bone and soft tissue in periodontal defects is highly desirable but remains challenging. A heparan sulphate variant (HS3) with enhanced affinity for bone morphogenetic protein-2 (BMP2) that, when combined with collagen or ceramic biomaterials, enhances bone tissue regeneration in the axial and cranial skeleton in several animal models was reported previously. In the current study, establishing the efficacy of a collagen/HS3 device for the regeneration of alveolar bone and the adjacent periodontal apparatus and related structures was sought. Collagen sponges loaded with phosphate-buffered saline, HS3, BMP2, or HS3 + BMP2 were implanted into surgically-created intra-bony periodontal defects in rat maxillae. At the 6 week end- point the maxillae were decalcified, and the extent of tissue regeneration determined by histomorphometrical analysis. The combination of collagen/HS3, collagen/BMP2 or collagen/HS3 + BMP2 resulted in a three to four-fold increase in bone regeneration and up to a 1.5 × improvement in functional ligament restoration compared to collagen alone. Moreover, the combination of collagen/HS3 + BMP2 improved the alveolar bone height and reduced the amount of epithelial growth in the apical direction. The implantation of a collagen/ HS3 combination device enhanced the regeneration of alveolar bone and associated periodontal tissues at amounts comparable to collagen in combination with the osteogenic factor BMP2. This study highlights the efficacy of a collagen/HS3 combination device for periodontal regeneration that warrants further development as a point-of-care treatment for periodontitis-related bone and soft tissue loss.

Tissue engineering in periodontology: Biological mediators for periodontal regeneration

2019 ◽  
Vol 42 (5) ◽  
pp. 241-257 ◽  
Author(s):  
Daniela Carmagnola ◽  
Gaia Pellegrini ◽  
Claudia Dellavia ◽  
Lia Rimondini ◽  
Elena Varoni
Keyword(s):  
Tissue Engineering ◽  
Periodontal Ligament ◽  
Alveolar Bone ◽  
State Of The Art ◽  
Periodontal Regeneration ◽  
Functional System ◽  
Bioactive Molecules ◽  
Comprehensive Review ◽  
Periodontal Tissues ◽  
Temporal And Spatial

Teeth and the periodontal tissues represent a highly specialized functional system. When periodontal disease occurs, the periodontal complex, composed by alveolar bone, root cementum, periodontal ligament, and gingiva, can be lost. Periodontal regenerative medicine aims at recovering damaged periodontal tissues and their functions by different means, including the interaction of bioactive molecules, cells, and scaffolds. The application of growth factors, in particular, into periodontal defects has shown encouraging effects, driving the wound healing toward the full, multi-tissue periodontal regeneration, in a precise temporal and spatial order. The aim of the present comprehensive review is to update the state of the art concerning tissue engineering in periodontology, focusing on biological mediators and gene therapy.

Regeneration of Periodontal Tissues in Non-human Primates with rhGDF-5 and Beta-Tricalcium Phosphate

2011 ◽  
Vol 90 (12) ◽  
pp. 1416-1421 ◽  
Author(s):  
K.B. Emerton ◽  
S.J. Drapeau ◽  
H. Prasad ◽  
M. Rohrer ◽  
P. Roffe ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Tricalcium Phosphate ◽  
Alveolar Bone ◽  
Periodontal Regeneration ◽  
Human Growth ◽  
Graft Material ◽  
Periodontal Tissue ◽  
Periodontal Tissues ◽  
Beta Tricalcium Phosphate ◽  
Periodontal Tissue Regeneration

The application of growth factors has been advocated in support of periodontal regeneration. Recombinant human growth and differentiation factor-5 (rhGDF-5), a member of the bone morphogenetic protein family, has been used to encourage periodontal tissue regeneration. This study evaluated the dose response of rhGDF-5 lyophilized onto beta-tricalcium phosphate (bTCP) granules for periodontal tissue regeneration in a baboon model. Periodontal defects were created bilaterally in 12 baboons by a split-mouth design. Plaque was allowed to accumulate around wire ligatures to create chronic disease. After 2 mos, the ligatures were removed, and a notch was placed at the base of the defect. Two teeth on each side of the mouth were randomly treated with bTCP only, 0.5, 1.0, or 2.0 mg rhGDF-5/g bTCP. Animals were sacrificed 5 mos post-treatment, with micro-CT and histomorphometric analysis performed. After 5 mos, analysis showed alveolar bone, cementum, and periodontal ligament formation in all treatment groups, with a dose-dependent increase in rhGDF-5-treated groups. Height of periodontal tissues also increased with the addition of rhGDF-5, and the amount of residual graft material decreased with rhGDF-5 treatment. Therefore, rhGDF-5 delivered on bTCP demonstrated effective regeneration of all 3 tissues critical for periodontal repair.

Tissue engineering in periodontics- A demystifing review

2021 ◽  
pp. 1-5
Author(s):  
Shivani Sachdeva ◽  
Harish Saluja ◽  
Amit Mani ◽  
M.B. Phadnaik
Keyword(s):  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Cell Biology ◽  
Alveolar Bone ◽  
Complete Recovery ◽  
Medical Sciences ◽  
Alternative Approach ◽  
Periodontal Tissue Regeneration ◽  
Novel Concept ◽  
And Function

INTRODUCTION: Novel concept known as tissue engineering is for the betterment of human. The use of much advanced molecular science and cell biology in processing the tissues to regenerate even after the loss of inborn tendency of pluripotent cells to multiply is possible by this new therapy. CONTENT: Periodontal tissue regeneration in both height and function is attributed to a complete recovery of the periodontal structures, that is, the formation of alveolar bone, a new connective attachment through collagen fibers as well as functionally oriented on the newly formed cementum is regeneration. Cell based therapies including tissue regeneration is an alternative approach for the regeneration of tissues damaged by disease or trauma. SUMMARY: Though tissue engineering requires the fundamentals of all the three keys namely genomics, proteomics and biometrics to give the solutions to biological problems appearing in dentistry as well as medical sciences.

scholarly journals Platelet-Rich Fibrin Promotes Periodontal Regeneration and Enhances Alveolar Bone Augmentation

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Qi Li ◽  
Shuang Pan ◽  
Smit J. Dangaria ◽  
Gokul Gopinathan ◽  
Antonia Kolokythas ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Alveolar Bone ◽  
Periodontal Regeneration ◽  
Principal Component ◽  
Nodule Formation ◽  
Bone Augmentation ◽  
Pilot Studies ◽  
Platelet Rich Fibrin ◽  
Lineage Differentiation ◽  
Osteogenic Lineage

In the present study we have determined the suitability of platelet-rich fibrin (PRF) as a complex scaffold for periodontal tissue regeneration. Replacing PRF with its major component fibrin increased mineralization in alveolar bone progenitors when compared to periodontal progenitors, suggesting that fibrin played a substantial role in PRF-induced osteogenic lineage differentiation. Moreover, there was a 3.6-fold increase in the early osteoblast transcription factor RUNX2 and a 3.1-fold reduction of the mineralization inhibitor MGP as a result of PRF application in alveolar bone progenitors, a trend not observed in periodontal progenitors. Subcutaneous implantation studies revealed that PRF readily integrated with surrounding tissues and was partially replaced with collagen fibers 2 weeks after implantation. Finally, clinical pilot studies in human patients documented an approximately 5 mm elevation of alveolar bone height in tandem with oral mucosal wound healing. Together, these studies suggest that PRF enhances osteogenic lineage differentiation of alveolar bone progenitors more than of periodontal progenitors by augmenting osteoblast differentiation, RUNX2 expression, and mineralized nodule formation via its principal component fibrin. They also document that PRF functions as a complex regenerative scaffold promoting both tissue-specific alveolar bone augmentation and surrounding periodontal soft tissue regeneration via progenitor-specific mechanisms.

scholarly journals Periodontal Bone-Ligament-Cementum Regeneration via Scaffolds and Stem Cells

Cells ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 537 ◽  
Author(s):  
Jin Liu ◽  
Jianping Ruan ◽  
Michael D. Weir ◽  
Ke Ren ◽  
Abraham Schneider ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Therapy ◽  
Tooth Loss ◽  
Soft Tissues ◽  
Alveolar Bone ◽  
Periodontal Regeneration ◽  
Periodontal Tissues ◽  
Oral And Maxillofacial Region ◽  
And Function ◽  
Bone Remains

Periodontitis is a prevalent infectious disease worldwide, causing the damage of periodontal support tissues, which can eventually lead to tooth loss. The goal of periodontal treatment is to control the infections and reconstruct the structure and function of periodontal tissues including cementum, periodontal ligament (PDL) fibers, and bone. The regeneration of these three types of tissues, including the re-formation of the oriented PDL fibers to be attached firmly to the new cementum and alveolar bone, remains a major challenge. This article represents the first systematic review on the cutting-edge researches on the regeneration of all three types of periodontal tissues and the simultaneous regeneration of the entire bone-PDL-cementum complex, via stem cells, bio-printing, gene therapy, and layered bio-mimetic technologies. This article primarily includes bone regeneration; PDL regeneration; cementum regeneration; endogenous cell-homing and host-mobilized stem cells; 3D bio-printing and generation of the oriented PDL fibers; gene therapy-based approaches for periodontal regeneration; regenerating the bone-PDL-cementum complex via layered materials and cells. These novel developments in stem cell technology and bioactive and bio-mimetic scaffolds are highly promising to substantially enhance the periodontal regeneration including both hard and soft tissues, with applicability to other therapies in the oral and maxillofacial region.

scholarly journals Electrospinning of Nanofibers for Tissue Engineering Applications

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Haifeng Liu ◽  
Xili Ding ◽  
Gang Zhou ◽  
Ping Li ◽  
Xing Wei ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Biomedical Applications ◽  
Fiber Morphology ◽  
Hard Tissue ◽  
Nanoscale Material ◽  
Considerable Potential ◽  
Recent Interest ◽  
Material Fabrication

Electrospinning is a method in which materials in solution are formed into nano- and micro-sized continuous fibers. Recent interest in this technique stems from both the topical nature of nanoscale material fabrication and the considerable potential for use of these nanoscale fibres in a range of applications including, amongst others, a range of biomedical applications processes such as drug delivery and the use of scaffolds to provide a framework for tissue regeneration in both soft and hard tissue applications systems. The objectives of this review are to describe the theory behind the technique, examine the effect of changing the process parameters on fiber morphology, and discuss the application and impact of electrospinning on the fields of vascular, neural, bone, cartilage, and tendon/ligament tissue engineering.

Sign in / Sign up

Export Citation Format

Share Document