scholarly journals Determination of Polyhexamethylene Biguanide Hydrochloride Using a Lactone-Rhodamine B-Based Fluorescence Optode

Molecules ◽  
2020 ◽  
Vol 25 (2) ◽  
pp. 262
Author(s):  
Akane Funaki ◽  
Yuta Horikoshi ◽  
Teruyuki Kobayashi ◽  
Takashi Masadome
Keyword(s):  
Fluorescence Intensity ◽  
Sodium Salt ◽  
Contact Lens ◽  
Rhodamine B ◽  
Determination Method ◽  
Polyhexamethylene Biguanide ◽  
Poly Vinyl Chloride ◽  
Nitrophenyl Octyl Ether ◽  
Optode Membrane

A new determination method for polyhexamethylene biguanide hydrochloride (PHMB) using a lactone-rhodamine B (L-RB) based fluorescence optode has been developed. The optode membrane consists of 2-nitrophenyl octyl ether as a plasticizer, L-RB, and poly (vinyl chloride). The optode responds to tetrakis (4-fluorophenyl) borate, sodium salt (NaTPBF) in the μM range. The fluorescence intensity of the L-RB film for PHMB solution containing 20 μM NaTPBF decreased linearly as the concentration of the PHMB solution increased in the concentration range from 0 to 8.0 μM, which shows that PHMB with a concentration range of 0 to 8.0 μM is determined by the L-RB film optode. The concentration of PHMB in the contact lens detergents by the proposed method was in accord with its nominal concentration.

scholarly journals Flow Cytometry for Determination of the Efficacy of Contact Lens Disinfecting Solutions againstAcanthamoeba spp

2000 ◽  
Vol 66 (3) ◽  
pp. 1057-1061 ◽  
Author(s):  
Roya N. Borazjani ◽  
Lauren L. May ◽  
Judith A. Noble ◽  
Simon V. Avery ◽  
Donald G. Ahearn
Keyword(s):  
Methylene Blue ◽  
Propidium Iodide ◽  
Contact Lens ◽  
Acanthamoeba Castellanii ◽  
Live Cells ◽  
Similar Data ◽  
Flow Cytometric ◽  
Polyhexamethylene Biguanide ◽  
Disinfectant Solution

ABSTRACT Flow cytometric analyses of cellular staining with fluorescent viability dyes and direct microscopic observations of methylene blue exclusion were compared for evaluation of the effects of a chlorhexidine gluconate-based contact lens disinfectant solution and a polyhexamethylene biguanide solution against cysts and trophozoites ofAcanthamoeba castellanii and Acanthamoeba polyphaga. The flow cytometric procedure with propidium iodide (used to stain dead cells) indicated that more than 90% of trophozoites of both species (inocula of 105 to 106/ml) at 22°C lost their viability after 4 h of exposure to chlorhexidine. When propidium iodide was used in combination with fluorescein diacetate (for live cells), the apparent number of propidium iodide-stained cells was reduced, but the relative efficacies of the two biguanide solutions appeared unchanged from those evident with the single dyes; the chlorhexidine solution was more effective than the polyhexamethylene biguanide solution. Similar data were obtained with the more cumbersome methylene blue exclusion procedure. Flow cytometric analyses provided a statistically reproducible and rapid procedure for determining the relative antiamoebal efficacies of the disinfecting solutions.

scholarly journals PAN-Immobilized PVC-NPOE Membrane for Environmentally Friendly Sensing of Cd(II) Ions

2017 ◽  
Vol 17 (1) ◽  
pp. 1 ◽  
Author(s):  
Moersilah Moersilah ◽  
Dwi Siswanta ◽  
Roto Roto ◽  
Mudasir Mudasir
Keyword(s):  
Water Samples ◽  
Limit Of Detection ◽  
Internal Standard ◽  
Environmentally Friendly ◽  
Aqueous System ◽  
Sensor Membrane ◽  
Poly Vinyl Chloride ◽  
Nitrophenyl Octyl Ether ◽  
Membrane Changes

A simple, cheap and environmentally friendly analytical method of Cd(II) in the aqueous system has been developed by immobilization of 1-(2-pyridilazo)-2-naphtol (PAN) in poly vinyl chloride (PVC) matrix and nitrophenyl octyl ether (NPOE) as a plasticizer. Upon contact with Cd(II) in solution, the color of sensor membrane changes from dark yellow to dark red, which is due to the formation of Cd(II)–PAN complex. The best sensing results were obtained at pH 8.0 and λmax 558 nm. The dimension of the proposed sensor membrane was 0.8 cm x 2 cm with a thickness of 0.05 mm, the volume of sample was 2 mL with the Cd(II) concentration range of  0 – 1.2 ppm. The limit of detection of the method was found to be 0.432 + 0.104 ppm, which was reversible. The proposed methods have been applied in the determination of Cd(II) in water samples after addition of internal standard.

scholarly journals Analytical Eco-Scale for Assessing the Greenness of a Developed Potentiometric Method for Lomefloxacin Hydrochloride Determination in its Different Dosage Forms, Plasma, and Dissolution Medium

2019 ◽  
Vol 102 (3) ◽  
pp. 794-800 ◽  
Author(s):  
Shereen A Boltia ◽  
Aya T Soudi ◽  
Eman S Elzanfaly ◽  
Hala E Zaazaa
Keyword(s):  
Dynamic Range ◽  
Low Cost ◽  
Sample Pretreatment ◽  
Dosage Forms ◽  
Hplc Method ◽  
Potentiometric Method ◽  
Poly Vinyl Chloride ◽  
Nitrophenyl Octyl Ether ◽  
Time Analyzer

Abstract Background: Traditional methods for Lomefloxacin hydrochloride (LOM) determination involve pretreatment steps, which extend analysis time and use hazardous chemicals. Objective: The ability to provide a rapid route without sample pretreatment for quantitative determination of compounds via a low-cost instrument is a challenging task. In this work, a simple potentiometric method was developed to determine the antibacterial LOM via in-house fabricated ion selective electrodes. Methods: Different sensors were fabricated using a poly vinyl chloride-based membrane, potassium tetrakis(4-chlorophenyl) borate as a cation exchanger, and 2-Nitrophenyl octyl ether as a plasticizer (sensor 1). To increase the selectivity of sensor 1, a selective molecular recognition component 2-hydroxypropyl-β-cyclodextrin was used as ionophore (sensor 2). Results: The proposed method was validated according to International Union of Pure and Applied Chemistry recommendations, in which the proposed sensors show a linear dynamic range from 1 × 10−5 to 1 × 10−2 mol/L, with Nernstian slopes of 55.829 and 58.229 mV/decade for sensors 1 and 2, respectively. It was applied to determine LOM in bulk powder, in different dosage forms, and in plasma with no sample pretreatment. Also, the suggested method can be used as a green, in-line bench top real-time analyzer for in-process monitoring of LOM release from its tablets, under U.S. Food and Drug Administration dissolution regulations, with clear discrimination from common excipients. Results obtained by the proposed potentiometric method were compared with those obtained by a reported HPLC method. Conclusions: The proposed method is considered as a perfect alternative to traditional reported methods for LOM determination.

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