scholarly journals Analysis of Primary Metabolites in Cabbage (Brassica oleracea var. capitata) Varieties Correlated with Antioxidant Activity and Taste Attributes by Metabolic Profiling

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4282
Author(s):  
Ryota Mabuchi ◽  
Mao Tanaka ◽  
Chihori Nakanishi ◽  
Nanako Takatani ◽  
Shota Tanimoto
Keyword(s):  
Antioxidant Activity ◽  
Metabolic Profiling ◽  
Metabolic Profile ◽  
Health Benefits ◽  
Electronic Tongue ◽  
Growth Conditions ◽  
Orthogonal Projections ◽  
Primary Metabolites ◽  
Latent Structures ◽  
Dpph Scavenging

Brassica vegetables, such as cabbage, have many health benefits arising from their antioxidant and anticancer properties. These properties are endowed by the metabolite composition of the plant, and it is therefore important to elucidate the metabolic profile and associated activities in this genus. This study objectively evaluated the characteristics of cabbage varieties using metabolic profiling to identify the primary metabolic components that correlate with antioxidant activity and taste attributes. GC-MS analysis was used to identify the primary metabolites. Antioxidant activity was measured by oxygen radical absorbance capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging assays, and an electronic tongue was used to quantitate nine taste attributes. Orthogonal projections to latent structures (OPLS) using SIMCA 14 correlated the metabolite components with the taste and antioxidant characteristics. We identified 4-aminobutyric acid, fructose 1-phosphate, adipic acid, 5-oxoproline, N-acetylglycine, O-phosphoethanolamine, and homovanillic acid as important determinants of DPPH scavenging activity and umami, sourness, acidic bitterness, irritant and saltiness, bitterness, astringency, and richness, respectively. These metabolites represent markers indicating breed differences and contribute to differential cabbage functionality. These studies could be extended to measure additional metabolites, as well as to understand the role of growth conditions on the metabolic profile and health benefits of plants.

scholarly journals Taste Evaluation of Yellowtail (Seriola Quinqueradiata) Ordinary and Dark Muscle by Metabolic Profiling

Molecules ◽  
2019 ◽  
Vol 24 (14) ◽  
pp. 2574 ◽  
Author(s):  
Mabuchi ◽  
Ishimaru ◽  
Adachi ◽  
Zhao ◽  
Kikutani ◽  
...  
Keyword(s):  
Metabolic Profiling ◽  
Evaluation Method ◽  
Pantothenic Acid ◽  
Electronic Tongue ◽  
Gas Chromatography Mass Spectrometry ◽  
Orthogonal Projections ◽  
Ms Analysis ◽  
Seriola Quinqueradiata ◽  
Taste Evaluation ◽  
Latent Structures

We performed metabolic profiling on yellowtail (Seriola quinqueradiata) muscle to develop an objective taste evaluation method for fish meat. Dark (DM) and ordinary (OM) muscle samples before and after storage were subjected to gas chromatography-mass spectrometry (GC-MS) analysis and taste measurements using an electronic tongue. The metabolites identified by the GC-MS analysis were treated as x variables, and the taste values obtained by the electronic tongue were treated as y variables. The relationships between the metabolites and taste attributes were evaluated by two-way orthogonal projections to latent structures (O2PLS) analysis. The O2PLS analyses were normalized in two ways, unit variance (UV) and pareto (Par) scaling. The O2PLS (UV) analysis produced 3+1+0 models in Autofit and this model was statistically significant with R2Y (0.73) and Q2 (0.52) metrics. In particular, significant correlations were found between DM or OM and metabolite intensity and taste attributes, and strong associations were found between “sourness” and lysine, “irritant” and alanine and phenylalanine, “saltiness” and pantothenic acid, and “umami” and creatinine and histidine. The O2PLS (Par) analysis of DM generated significant predictive models for “acidic bitterness,” “irritant,” “saltiness,” “bitterness,” “astringency,” and “richness.” Among these, only “irritant” was affected by storage. This method was thus effective in evaluating the taste of yellowtail muscle.

scholarly journals The Influence of Drying Temperatures on the Metabolic Profiles and Antioxidant Activity of Manilkara zapota Leaves

Metabolites ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 217 ◽  
Author(s):  
Hernández-Bolio ◽  
Dzul-Romero ◽  
Velázquez ◽  
Cresencio ◽  
Hernández-Núñez ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
1H Nmr ◽  
Metabolic Profile ◽  
Total Phenol Content ◽  
Orthogonal Projections ◽  
Southern Mexico ◽  
Manilkara Zapota ◽  
Latent Structures ◽  
Drying Efficiency ◽  
The Impact

In the present study, the leaves of Manilkara zapota (L.) P. Royen (Sapotaceae), an evergreen tree recognized for its medicinal properties in Southern Mexico, were used as a model to study the effect of different drying temperatures on its metabolic profile and therefore, its antioxidant potential. For this purpose, a methanol extraction of leaves dried at room temperature (25 °C) or by heat convection (50, 75 and 100 °C) were compared in terms of drying efficiency, yield of extraction, total phenol content, 1H-NMR metabolic profile, and DPPH antioxidant activity. The drying curves enabled the fact to be uncovered that drying efficiency improves with increase of temperature, as does the level of total phenols and antioxidant activity. A metabolomics approach using principal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA) of the corresponding 1H-NMR profiles allowed the impact of the drying temperature on their metabolic profile to be documented and also, caffeic acid and epicatechin as main secondary metabolites contributing to the antioxidant activity of M. zapota to be identified.

scholarly journals Significant Changes in Metabolic Profiles after Intervention with Selenium and Coenzyme Q10 in an Elderly Population

Biomolecules ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 553
Author(s):  
Alehagen ◽  
Johansson ◽  
Aaseth ◽  
Alexander ◽  
Surowiec ◽  
...  
Keyword(s):  
Cardiovascular Mortality ◽  
Coenzyme Q10 ◽  
Metabolic Profiling ◽  
Demographic Data ◽  
Principal Component ◽  
Elderly Persons ◽  
Double Blind ◽  
Orthogonal Projections ◽  
Healthy Elderly ◽  
Latent Structures

Selenium and coenzyme Q10 (SeQ10) are important for normal cellular function. Low selenium intake leads to increased cardiovascular mortality. Intervention with these substances with healthy elderly persons over a period of four years in a double-blind, randomised placebo-controlled prospective study showed reduced cardiovascular mortality, increased cardiac function, and a lower level of NT-proBNP. Therefore, we wanted to evaluate changes in biochemical pathways as a result of the intervention with SeQ10 using metabolic profiling. From a population of 443 healthy elderly individuals that were given 200 µg selenium and 200 mg coenzyme Q10, or placebo daily for four years, we selected nine males on active intervention and nine males on placebo for metabolic profiling in the main study. To confirm the results, two validation studies (study 1 n = 60 males, study 2 n = 37 males) were conducted. Principal component analyses were used on clinical and demographic data to select representative sets of samples for analysis and to divide the samples into batches for analysis. Gas chromatography time-of-flight mass spectrometry-based metabolomics was applied. The metabolite data were evaluated using univariate and multivariate approaches, mainly T-tests and orthogonal projections to latent structures (OPLS) analyses. Out of 95 identified metabolites, 19 were significantly decreased due to the intervention after 18 months of intervention. Significant changes could be seen in the pentose phosphate, the mevalonate, the beta-oxidation and the xanthine oxidase pathways. The intervention also resulted in changes in the urea cycle, and increases in the levels of the precursors to neurotransmitters of the brain. This adds information to previous published results reporting decreased oxidative stress and inflammation. This is the first-time metabolic profiling has been applied to elucidate the mechanisms behind an intervention with SeQ10. The study is small and should be regarded as hypothesis-generating; however, the results are interesting and, therefore, further research in the area is needed. This study was registered at Clinicaltrials.gov, with the identifier NCT01443780

NMR Metabolomics Investigates the Influence of Flavonoid-Enriched Rations on Chicken Plasma

2017 ◽  
Vol 100 (2) ◽  
pp. 315-322 ◽  
Author(s):  
Charalambos Fotakis ◽  
Dimitra Z Lantzouraki ◽  
Michael Goliomytis ◽  
Panagiotis E Simitzis ◽  
Maria Charismiadou ◽  
...  
Keyword(s):  
Vitamin E ◽  
Metabolic Profile ◽  
Serum Samples ◽  
Orthogonal Projections ◽  
Nmr Metabolomics ◽  
Projections To Latent Structures ◽  
Latent Structures ◽  
Chicken Blood ◽  
Control Samples ◽  
Acetic Acids

Abstract The use of flavonoids as dietary supplements is well established, mainly due to their intense antioxidant and anti-inflammatory properties. In the present study, hesperidin, naringin, and vitamin E were used as additives at different concentrations in poultry rations in order to achieve meat of improved quality. NMR metabolomics was applied to chicken blood serum samples to discern whether and how the enriched rations affected the animals' metabolic profile. Variations in the metabolic patterns according to sustenance consumption were tracedby orthogonal projections to latent structures discriminant analysis (OPLS-DA) models and were attributed to specific metabolites by using S-line plots. In particular, serum samples from chickens fed with vitamin E displayed higher concentrations of glycine and succinic acid compared to control samples, which were mainly characterized by betaine, formic acid, and lipoproteins. Samples from chickens fed with hesperidin were characterizedby increased levels of lactic acid, citric acid, creatine, carnosine, creatinine, phosphocreatine, anserine, glucose, and alanine compared to control samples. Lastly, naringin samples exhibited increased levels of citric and acetic acids. Results verify the scalability of NMR metabolomics to highlight metabolite variations among chicken serum samples in relation to food rations.

scholarly journals Application of Metabolomics in Traditional Chinese Medicine Differentiation of Deficiency and Excess Syndromes in Patients with Diabetes Mellitus

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Tao Wu ◽  
Ming Yang ◽  
Hua-Feng Wei ◽  
Song-Hua He ◽  
Shun-Chun Wang ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Metabolic Profiling ◽  
Gas Chromatography Mass Spectrometry ◽  
Multivariate Statistical ◽  
Orthogonal Projections ◽  
Patients With Diabetes ◽  
Projections To Latent Structures ◽  
Latent Structures

Metabolic profiling is widely used as a probe in diagnosing diseases. In this study, the metabolic profiling of urinary carbohydrates was investigated using gas chromatography/mass spectrometry (GC/MS) and multivariate statistical analysis. The kernel-based orthogonal projections to latent structures (K-OPLS) model were established and validated to distinguish between subjects with and without diabetes mellitus (DM). The model was combined with subwindow permutation analysis (SPA) in order to extract novel biomarker information. Furthermore, the K-OPLS model visually represented the alterations in urinary carbohydrate profiles of excess and deficiency syndromes in patients with diabetes. The combination of GC/MS and K-OPLS/SPA analysis allowed the urinary carbohydrate metabolic characterization of DM patients with different traditional Chinese medicine (TCM) syndromes, including biomarkers different from non-DM patients. The method presented in this study might be a complement or an alternative to TCM syndrome research.

Metabolic profiling and antioxidant activity of Sideritis species growing in southeast europe

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
G Papaefstathiou ◽  
N Aligiannis ◽  
N Fokialakis ◽  
L Skaltsounis
Keyword(s):  
Antioxidant Activity ◽  
Metabolic Profiling ◽  
Southeast Europe

scholarly journals Bioactive Compounds, Antioxidant Activity and Growth Behavior in Lettuce Cultivars Grown under Field and Greenhouse Conditions

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 52
Author(s):  
Melisa Lanza Volpe ◽  
Verónica C. Soto Vargas ◽  
Anabel Morón ◽  
Roxana E. González
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Growth Conditions ◽  
Growth Behavior ◽  
Total Phenolic ◽  
Spectrophotometric Methods ◽  
Leafy Greens ◽  
Growing Conditions ◽  
Field Production

Lettuce (Lactuca sativa L.) is one of the most important leafy greens worldwide. The nutritional value of its edible leaf depends on different factors including type and growing conditions. The aim was to determine the bioactive compounds content, antioxidant activity and growth behavior of twenty-two lettuce genotypes, cultivated under field and greenhouse conditions. Total phenolic compound, chlorophylls, carotenoids, anthocyanin contents and antioxidant activities were analyzed by spectrophotometric methods. Data were analyzed by analysis of variance (ANOVA). Significant differences between bioactive compounds, antioxidant activity and growth behavior were found among cultivars and morphological types, for both growth conditions. Carotenoid and chlorophyll content was higher in greenhouse conditions for all genotypes. In field production, butterhead and iceberg lettuces showed lower content of these bioactive compounds. The red-pigmented Falbala cultivar from field production showed the highest level of polyphenols and anthocyanin. Meanwhile, in greenhouse conditions, the oak leaf cultivar Grenadine displayed the highest concentration of these phenolic compounds. The iceberg type lettuce showed the lowest percentages of antioxidant activity in both environments. The results showed the effect of growing conditions and the high variability in lettuce bioactive compounds content and antioxidant activity among the different types.

scholarly journals Optimization of Ultrasonic Cellulase-Assisted Extraction and Antioxidant Activity of Natural Polyphenols from Passion Fruit

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2494
Author(s):  
Wei Wang ◽  
Yu-Ting Gao ◽  
Ji-Wen Wei ◽  
Yin-Feng Chen ◽  
Qing-Lei Liu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Extraction Method ◽  
Ph Value ◽  
Soxhlet Extraction ◽  
Passion Fruit ◽  
Total Phenol Content ◽  
Extraction Temperature ◽  
Scavenging Activity ◽  
Solid Ratio ◽  
Dpph Scavenging

In this paper, ultrasonic cellulase extraction (UCE) was applied to extract polyphenols from passion fruit. The extraction conditions for total phenol content (TPC) and antioxidant activity were optimized using response surface methodology (RSM) coupled with a Box-Behnken design (BBD). The results showed that the liquid-to-solid ratio (X2) was the most significant single factor and had a positive effect on all responses. The ANOVA analysis indicated quadratic models fitted well as TPC with R2 = 0.903, DPPH scavenging activity with R2 = 0.979, and ABTS scavenging activity with R2 = 0.981. The optimal extraction parameters of passion fruit were as follows: pH value of 5 at 30 °C for extraction temperature, 50:1 (w/v) liquid-to-solid ratio with extraction time for 47 min, the experimental values were found matched with those predicted. Infrared spectroscopy suggested that the extract contained the structure of polyphenols. Furthermore, three main polyphenols were identified and quantified by HPLC. The results showed the content of phenolic compounds and antioxidant activity of the optimized UCE were 1.5~2 times higher than that determined by the single extraction method and the Soxhlet extraction method, which indicates UCE is a competitive and effective extraction technique for natural passion fruit polyphenols.

scholarly journals Influence of the metabolic profile on the in vivo antioxidant activity of quercetin under a low dosage oral regimen in rats

2008 ◽  
Vol 153 (8) ◽  
pp. 1750-1761 ◽  
Author(s):  
M R Santos ◽  
M J Rodríguez-Gómez ◽  
G C Justino ◽  
N Charro ◽  
M H Florencio ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Metabolic Profile ◽  
Oral Regimen ◽  
Low Dosage
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