scholarly journals Identification and Extraction Optimization of Active Constituents in Citrus junos Seib ex TANAKA Peel and Its Biological Evaluation

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 680 ◽  
Author(s):  
Jung-hyun Shim ◽  
Jung-il Chae ◽  
Seung-sik Cho
Keyword(s):  
Biological Activities ◽  
Biological Effects ◽  
Ethanolic Extract ◽  
Biological Evaluation ◽  
Water Soluble ◽  
Hot Water ◽  
Aging Effects ◽  
Active Constituents ◽  
Citrus Junos ◽  
Ethanolic Extracts

Citrus junos Seib ex TANAKA possesses various biological effects. It has been used in oriental remedies for blood circulation and the common cold. Recently, biological effects of C. junos peel have been reported. However, optimization of the biological properties of C. junos peel preparations has yet to be reported on. We developed a high-performance liquid chromatography (HPLC) method for quantification of the active constituents in C. junos peel. Hot water and ethanolic extracts of C. junos peel were prepared and their chemical profiles and biological activities were evaluated. The 80% ethanolic extract demonstrated the greatest antioxidant activity and phenolic content, while the 100% ethanolic extract had the greatest xanthine oxidase inhibitory activity. Elastase inhibition activity was superior in aqueous and 20% ethanolic extracts. The contents of two flavonoids were highest in the 100% ethanolic extract. We postulated that the antioxidant and anti-aging effects of C. junos peel extract could be attributed to phenolics such as flavonoids. Our results suggest that the flavonoid-rich extract of C. junos may be utilized for the treatment and prevention of metabolic disease and hyperuricemia while the water-soluble extract of C. junos could be used as a source for its anti-aging properties.

scholarly journals Metabolic Profiling of Water-Soluble Compounds from the Extracts of Dark Septate Endophytic Fungi (DSE) Isolated from Scots Pine (Pinus sylvestris L.) Seedlings Using UPLC–Orbitrap–MS

Molecules ◽  
2019 ◽  
Vol 24 (12) ◽  
pp. 2330 ◽  
Author(s):  
Jenni Tienaho ◽  
Maarit Karonen ◽  
Riina Muilu–Mäkelä ◽  
Kristiina Wähälä ◽  
Eduardo Leon Denegri ◽  
...  
Keyword(s):  
Amino Acids ◽  
Host Plant ◽  
Scots Pine ◽  
Biological Activities ◽  
Water Soluble ◽  
Hot Water ◽  
Metabolic Profiles ◽  
Ionization Source ◽  
Pine Seedlings ◽  
Ultrahigh Performance Liquid Chromatography

Endophytes are microorganisms living inside plant hosts and are known to be beneficial for the host plant vitality. In this study, we isolated three endophytic fungus species from the roots of Scots pine seedlings growing on Finnish drained peatland setting. The isolated fungi belonged to dark septate endophytes (DSE). The metabolic profiles of the hot water extracts of the fungi were investigated using Ultrahigh Performance Liquid Chromatography with Diode Array Detection and Electron Spray Ionization source Mass Spectrometry with Orbitrap analyzer (UPLC–DAD–ESI–MS–Orbitrap). Out of 318 metabolites, we were able to identify 220, of which a majority was amino acids and peptides. Additionally, opine amino acids, amino acid quinones, Amadori compounds, cholines, nucleobases, nucleosides, nucleotides, siderophores, sugars, sugar alcohols and disaccharides were found, as well as other previously reported metabolites from plants or endophytes. Some differences of the metabolic profiles, regarding the amount and identity of the found metabolites, were observed even though the fungi were isolated from the same host. Many of the discovered metabolites have been described possessing biological activities and properties, which may make a favorable contribution to the host plant nutrient availability or abiotic and biotic stress tolerance.

scholarly journals Beneficial Health Potential of Algerian Polysaccharides Extracted from Plantago ciliata Desf. (Septentrional Sahara) Leaves and Seeds

2021 ◽  
Vol 11 (9) ◽  
pp. 4299
Author(s):  
Noura Addoun ◽  
Zakaria Boual ◽  
Cédric Delattre ◽  
Toufik Chouana ◽  
Christine Gardarin ◽  
...  
Keyword(s):  
Innate Immune System ◽  
Biological Activities ◽  
Structural Features ◽  
Traditional Healers ◽  
Innate Immune ◽  
Water Soluble ◽  
Hot Water ◽  
Indigenous Plants ◽  
Hot Water Extraction ◽  
Anti Inflammatory

Today, an ethnobotanical approach makes sense for identifying new active bioactive chemicals from uses of indigenous plants. Two water-soluble enriched polysaccharide fractions (L-PSPN and S-PSPN) were extracted by hot water extraction from the leaves and seeds of Plantago ciliata Desf. (1798), a Mzab indigenous herb currently used in Algeria by traditional healers. Primary investigation was performed for describing the main structural features of these polysaccharides (pectin- and heteroxylan-like compositions) by using colorimetric assays, FTIR spectroscopy, HPAEC/PAD and GC/MS-EI analyses. Some biological activities were also monitored, such as anticomplement, anti-inflammatory (phagocytic ability, NOX2 and MPO inhibitions) and anti-diabetic (α-amylase and α-glucosidase inhibitions). L-PSPC seems able to moderately modulate innate immune system (IC50 around 100 µg/mL) and contribute to wound-healing processes (IC50 close to 217 vs. 443 µg/mL for sodium heparin). S-PSPC shows some potential as an anti-hyperglycemic (IC50 around 4.7 mg/mL) and anti-inflammatory (IC50 ranging from 111 to 203 µg/mL) agent, as well as other (fiber) psyllium-like polysaccharides extracted from Plantago species.

Biochemical Activities of Propolis Extracts. I. Standardization and Antioxidative Properties of Ethanolic and Aqueous Derivatives

1993 ◽  
Vol 48 (11-12) ◽  
pp. 851-857 ◽  
Author(s):  
Regina Volpert ◽  
Erich F Elstner
Keyword(s):  
Indole Acetic Acid ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Water Soluble ◽  
Biochemical Activity ◽  
Ethanolic Extracts ◽  
Ancient Times ◽  
Radical Scavenging Properties ◽  
Catalyzed Oxidation

Abstract Ethanolic extracts of Propolis are used as antiinflammatory and wound healing drugs since ancient times. In order to facilitate a comparison of different extracts, the standardization on the basis of quantitative determination of prominent components of these extracts has been substituted for simple biochemical “activity” tests. One of these activity tests bases on the in­ hibition of peroxidase-catalyzed oxidation of indole acetic acid indicating the presence of a defined mixture of monophenolic and diphenolic compounds. Other tests (diaphorase-catalyzed reductions and xanthine oxidase-catalyzed oxidations) demonstrate significant radical scavenging properties. Water-soluble extracts of propolis exhibit higher antioxidative and inhibitory activities as compared to the ethanolic extract.

Structure and Bioactivities of Porphyrans and Oligoporphyrans

2019 ◽  
Vol 25 (11) ◽  
pp. 1163-1171 ◽  
Author(s):  
Lihua Geng ◽  
Jing Wang ◽  
Zhongshan Zhang ◽  
Yang Yue ◽  
Quanbin Zhang
Keyword(s):  
Drug Delivery ◽  
Therapeutic Potential ◽  
Biological Activities ◽  
Great Influence ◽  
Physical Method ◽  
Extraction Process ◽  
Extraction Methods ◽  
Sulfated Polysaccharide ◽  
Water Soluble ◽  
Hot Water

Background: Pyropia (Porphyra), commonly known as nori or laver, is an important food source in many parts of the world. Edible dried Pyropia contains numerous nutrients and biofunctional components, including proteins, vitamins, eicosapentaenoic acid, minerals, carotenoids, mycosporine-like amino acids, and carbohydrate, and one of the compounds which we are interested in is porphyran, a sulfated polysaccharide comprising the hot-water-soluble portion of Pyropia cell walls. Researchers have performed a large number of in-depth studies on the biological activity and potential therapeutic applications of porphyrans and oligoporphyrans. Methods: This mini review aims to provide comprehensive and update overview on the source, extraction, structure, biological activities and structure-activity relationships of porphyrans and oligoporphyrans based on the studies in the past 30 years which were included in Web of Science. Results: The structure of porphyran has been basically determined given that its straight chain is relatively simple, and the skeleton structure has been described. The extraction methods were simplified continuously, but different extraction methods and post- processing methods still had great influence on the structure and composition of porphyran, so there was no standardized extraction process which can achieve quality control until now. In order to obtain oligoporphyrans, there are a variety of degradation methods, including chemical method, physical method and enzymatic method, but it is worth mentioning that specific degradation enzyme is still unavailable. Studies on the biological and pharmacology properties include antioxidant, anti-tumor, anti-inflammatory, immunomodulation, anti-cardiovascular and cerebrovascular diseases and drug delivery. Conclusion: Owing to the therapeutic potential and drug delivery applications, porphyran and oligoporphyrans are expected to be further developed as a medicine against human diseases, as well as a supplement in cosmetics and health products.

scholarly journals HPLC Analysis and Antioxidant Evaluation of Acteoside-Rich Osmanthus fragrans Extracts

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Sung-Ho Lee ◽  
Seong-Wook Seo ◽  
Seung-Yub Song ◽  
Deuk-Sil Oh ◽  
Jung-Hyun Shim ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Hot Water ◽  
Total Phenolic ◽  
Osmanthus Fragrans

A method for the separation and quantification of acteoside by reverse-phase high-performance liquid chromatography (HPLC) was developed and validated. Hot water and ethanolic extracts of Osmanthus fragrans leaves and flowers were analyzed for acteoside content. Excellent linearity was obtained, with an r2 higher than 0.999. The precision, specificity, and accuracy of our method were excellent, suggesting that it can be conveniently used for the quantification of acteoside in the crude extract of O. fragrans. The hot water and ethanol extracts were analyzed, and their biological activities were tested. The extraction yields, marker (acteoside) contents, and antioxidant activities of the leaf and flower extracts were analyzed. The antioxidant activity was confirmed by measuring the 2,2-diphenyl-2-picrylhydrazyl radical scavenging activity, reducing power, and total phenolic content. The acteoside content tended to be higher in the 100% ethanol extract of O. fragrans compared to those with the other extraction conditions tested. Overall, almost all extracts prepared with ethanolic solvents tended to produce better antioxidant activity than those prepared with hot water. These results suggest that the ethanolic extract of O. fragrans could serve as a potential antioxidant and anti-inflammatory pharmaceutical source, and our validated method would be useful for the quality control of O. fragrans extracts.

scholarly journals Phytochemical and Biological Evaluation of Justica adhatoda

2017 ◽  
Vol 9 (1) ◽  
pp. 10
Author(s):  
Shahid Aziz ◽  
Hidayat Hussain ◽  
Anisa Younis ◽  
Habib Ur-Rehman ◽  
Muhammad Shahid ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Cardiac Glycosides ◽  
Reducing Sugars ◽  
Ethanolic Extract ◽  
Biological Evaluation ◽  
Root Bark ◽  
Antibacterial And Antifungal Activities ◽  
Ethanolic Extracts ◽  
Antibacterial And Antifungal

<p>Phytochemical and biological investigations were undertaken on  ethanolic extracts of the leaves and root bark of <em>Justica adhatoda </em>L<em>.</em>  Alkaloids, cardiac glycosides, tannins, steroids and saponins were detected in  extracts from both parts of the title plant. Reducing sugars  were not detected in  either extract whereas flavonoids were  detected in ethanolic extract of <em>J. adhatota </em>leaves only. Significant antibacterial and antifungal activities against <em>Escherichia coli</em>, <em>Staphylococcus aureus</em> and Aspergillus <em>niger</em>  were demonstrated by both the  leaf and root bark extracts. Additionally, both extracts  displayed good antioxidant and heomolytic potential.  Interestingly, eight metal ions were detected in both extracts  in different concentrations.</p>

scholarly journals In-vitro Testing of Antioxidant, Anti-Parasite Activities, Cytotoxicity, and Chemical Evaluation of Abutilon Pannosum and Cassia Occidentalis Ethanolic Extracts

2021 ◽  
pp. 112-121
Author(s):  
Ahmed A. Elshikh ◽  
Mawahib E. M. ElNour ◽  
Hatil H. Elkamali ◽  
Ahmed S. Kabbashi
Keyword(s):  
Cell Line ◽  
Brine Shrimp ◽  
Normal Cell ◽  
Biological Activities ◽  
Ethanolic Extract ◽  
Toxicity Tests ◽  
Cassia Occidentalis ◽  
Normal Cell Line ◽  
Ethanolic Extracts

Aims: The aim of this study to detect Anti-giardia, antioxidant activities, cytotoxicity and evaluated the chemical constituent of ethanolic extracts of Abutilon pannosum and Cassia occidentalis. Study Design:  Various standard methods were used to detect of bioactivity for ethanolic extracts of plants used in this study. Place and Duration of Study: This study was conducted in the laboratories of microbiology and parasitology and chemistry, the International University of Africa, Khartoum, Sudan, during May 2019. Methodology: The ethanolic extract of Abutilon pannosum and Cassia occidentalis was used as an anti-giardia and anti-oxidant in-vitro, and toxicity tests were performed using brine shrimp and MTT assay. Also, the compounds of the plants used were detected by the GCMS apparatus. Results: The ethanolic extracts of Abutilon pannosum showed high Anti-giardia activity (79%) in concentration (500 ppm) after 72 hours, whereas the activity of Cassia occidentalis extract showed (61%). The highest antioxidant activity of ethanolic extract of Cassia occidentalis was (68.7%), while it was weak in Abutilon pannosum ethanolic extract (45%) by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The results of cytotoxicity revealed that the ethanolic extracts are highly toxic to brine shrimp, but are not toxic to normal cell line (MTT). Chromatographic analysis using gas chromatography-mass spectroscopy (GCMS) showed good separation of compounds. GCMS detected 22 and14 important compounds in Abutilon pannosum and Cassia occidentalis extracts respectively. The common compound in both plant extracts is n-Hexadecanoic acid. This acid was reported as an antioxidant. Conclusion: This study revealed that the biological activities of Abutilon pannosum extracts showed high activities of Anti-giardia and antioxidants. Non-cytotoxic in the normal cell line was shown. Cassia occidentalis showed high activity of Anti-giardia and weak activity antioxidant.

scholarly journals Biological activities of marine invertebrates extracts from the northeast brazilian coast

2020 ◽  
Vol 80 (2) ◽  
pp. 393-404 ◽  
Author(s):  
C. Lhullier ◽  
M. I. G. Moritz ◽  
E. O. Tabalipa ◽  
F. N. Sardá ◽  
N. F. Z. Schneider ◽  
...  
Keyword(s):  
Biological Activities ◽  
Ethanolic Extract ◽  
Marine Sponges ◽  
Diffusion Method ◽  
Brazilian Coast ◽  
Isolation And Identification ◽  
Disk Diffusion Method ◽  
Antiproliferative Effects ◽  
Ethanolic Extracts ◽  
Hsv 1

Abstract This paper reports the in vitro antiproliferative effects, antiprotozoal, anti-herpes and antimicrobial activities of 32 organic extracts of 14 marine sponges and 14 corals collected in northeast Brazilian coast. The ethanolic extracts of the sponges Amphimedon compressa and Tedania ignis, and the acetone extract of Dysidea sp. showed relevant results concerning the antiproliferative effects against A549, HCT-8, and PC-3 cell lines by sulforhodamine B assay, but also low specificity. Concerning the antiprotozoal screening, the ethanolic extract of Amphimedon compressa and the acetone and ethanolic extracts of Dysidea sp. were the most active against Leishmania amazonensis and Trypanosoma cruzi expressing β-galactosidase in THP-1 cells. In the preliminary anti-HSV-1 (KOS strain) screening, the ethanolic extracts of the sponges Amphimedon compressa, Haliclona sp. and Chondrosia collectrix inhibited viral replication by more than 50%. The most promising anti-herpes results were observed for the ethanolic extract of Haliclona sp. showing high selective indices against HSV-1, KOS and 29R strains (SI> 50 and >79, respectively), and HSV-2, 333 strain (IS>108). The results of the antibacterial screening indicated that only the ethanolic extract of Amphimedon compressa exhibited a weak activity against Enterococcus faecalis, Pseudomonas aeruginosa and Escherichia coli by the disk diffusion method. In view of these results, the extracts of Amphimedon compressa, Tedania ignis and Dysidea sp. were selected for further studies aiming the isolation and identification of the bioactive compounds with antiproliferative and/or antiprotozoal activities. The relevant anti-herpes activity of the ethanolic extract of Haliclona sp. also deserves special attention, and will be further investigated.

scholarly journals Studying of analgesic and anti-inflammatory activity of 2,4-dioxobutanoic acid hydrazine derivatives

2020 ◽  
Vol 37 (4) ◽  
pp. 115-119
Author(s):  
Natalia A. Pulina ◽  
Aleksander S. Kuznecov ◽  
Svetlana V. Chashchina
Keyword(s):  
Biological Activities ◽  
Biological Effects ◽  
Water Soluble ◽  
Inflammatory Activity ◽  
Synthesis Methods ◽  
Hot Plate Test ◽  
Hydrazine Derivatives ◽  
Anti Inflammatory ◽  
The Relationship ◽  
Derivatives Of

Objective. To study the analgesic and anti-inflammatory activity of water-soluble hydrazine derivatives of 2,4-dioxobutanoic acids, as well as to determine the relationship between the structure of substances and their biological effects. Materials and methods. The laboratory synthesis methods were applied to obtain 2-hydrazine derivatives of 2,4-dioxobutanoic acids. The compounds were tested for biological activity using a hot plate test in mice and an acute inflammatory reaction caused by carrageenan-induced paw edema in rats. Results. The analgesic activity of four compounds is comparable to that of nimesulide. One of the substances obtained is found to exhibit higher anti-inflammatory activity than nimesulide during the 1st and 3rd hours of the experiment. Two compounds with a combination of high analgesic and anti-inflammatory activity were identified. The effect of certain radicals in the structure of the substances on the activities studied was discovered. Conclusions. The revealed relationship between the structure of original compounds and their biological activities can be used in the further synthesis and search for new domestic pharmaceutical substances with investigated effects.

scholarly journals Antimicrobial and antioxidant activity of essential oil and different plant extracts of Psidium cattleianum Sabine

2016 ◽  
Vol 76 (1) ◽  
pp. 101-108 ◽  
Author(s):  
M. C. Scur ◽  
F. G. S. Pinto ◽  
J. A. Pandini ◽  
W. F. Costa ◽  
C. W. Leite ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Plant Extracts ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Ethanolic Extract ◽  
Phytochemical Screening ◽  
Psidium Cattleianum ◽  
Ethanolic Extracts

Abstract The goals of the study were to determinethe antimicrobial and antioxidant activities of essential oil and plant extracts aqueous and ethanolic of Psidium cattleianum Sabine; the chemical composition of the essential oil of P. cattleianum; and the phytochemical screening of aqueous and ethanolic extracts of the same plant. Regarding the antimicrobial activity, the ethanolic extract exhibited moderate antimicrobial activity with respect to bacteria K. pneumoniae and S. epidermidis, whereas, regarding other microorganisms, it showed activity considered weak. The aqueous extract and the essential oil showed activity considered weak, although they inhibited the growth of microorganisms. About the antioxidant potential, the ethanolic and aqueous extracts exhibited a scavenging index exceeding 90%, while the essential oil didn´t show significant antioxidant activity. Regarding the phytochemical composition, the largest class of volatile compounds identified in the essential oil of P. cattleianum included the following terpenic hydrocarbons: α-copaene (22%); eucalyptol (15%), δ-cadinene (9.63%) and α-selinene (6.5%). The phytochemical screening of extracts showed the presence of tannins, flavonoids, and triterpenoids for aqueous and ethanolic extracts. The extracts and essential oils inhibit the growth of microrganisms and plant extracts showed significant antioxidant activity. Also, the phytochemical characterization of the essential oil showed the presence of compounds interest commercial, as well as extracts showed the presence of important classes and compounds with biological activities.

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