scholarly journals Chemical Constituents from the Stems of Tinospora sinensis and Their Bioactivity

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2541 ◽  
Author(s):  
Sio-Hong Lam ◽  
Po-Hsun Chen ◽  
Hsin-Yi Hung ◽  
Tsong-Long Hwang ◽  
Chih-Chao Chiang ◽  
...  
Keyword(s):  
Analytical Methods ◽  
Superoxide Anion ◽  
Cytochalasin B ◽  
Chemical Constituents ◽  
Superoxide Anion Generation ◽  
Anti Inflammatory ◽  
New Compounds

Fifty-seven compounds were purified from the stems of Tinospora sinensis, including three new compounds characterized as a lignan (1), a pyrrole alkaloid (11), and a benzenoid (17), respectively. Their structures were elucidated and established by various spectroscopic and spectrometric analytical methods. Among the isolates, fifteen compounds were examined for their anti-inflammatory potential in vitro. The results showed that several compounds displayed moderate inhibition of N-formyl-methionyl-leucyl-phenylalanine/cytochalasin B (fMLP/CB)-induced superoxide anion generation and elastase release.

scholarly journals An Anti-Inflammatory 2,4-Cyclized-3,4-Secospongian Diterpenoid and Furanoterpene-Related Metabolites of a Marine Sponge Spongia sp. from the Red Sea

Marine Drugs ◽  
2021 ◽  
Vol 19 (1) ◽  
pp. 38
Author(s):  
Chi-Jen Tai ◽  
Chiung-Yao Huang ◽  
Atallah F. Ahmed ◽  
Raha S. Orfali ◽  
Walied M. Alarif ◽  
...  
Keyword(s):  
Red Sea ◽  
Superoxide Anion ◽  
Dermal Fibroblast ◽  
Fibroblast Cell ◽  
Inhibitory Effect ◽  
Human Dermal Fibroblast ◽  
Superoxide Anion Generation ◽  
Potent Inhibitory Effect ◽  
Anti Inflammatory ◽  
New Compounds

Chemical investigation of a Red Sea Spongia sp. led to the isolation of four new compounds, i.e., 17-dehydroxysponalactone (1), a carboxylic acid, spongiafuranic acid A (2), one hydroxamic acid, spongiafuranohydroxamic acid A (3), and a furanyl trinorsesterpenoid 16-epi-irciformonin G (4), along with three known metabolites (−)-sponalisolide B (5), 18-nor- 3,17-dihydroxy-spongia-3,13(16),14-trien-2-one (6), and cholesta-7-ene-3β,5α-diol-6-one (7). The biosynthetic pathway for the molecular skeleton of 1 and related compounds was postulated for the first time. Anti-inflammatory activity of these metabolites to inhibit superoxide anion generation and elastase release in N-formyl-methionyl-leucyl phenylalanine/cytochalasin B (fMLF/CB)-induced human neutrophil cells and cytotoxicity of these compounds toward three cancer cell lines and one human dermal fibroblast cell line were assayed. Compound 1 was found to significantly reduce the superoxide anion generation and elastase release at a concentration of 10 μM, and compound 5 was also found to display strong inhibitory activity against superoxide anion generation at the same concentration. Due to the noncytotoxic activity and the potent inhibitory effect toward the superoxide anion generation and elastase release, 1 and 5 can be considered to be promising anti-inflammatory agents.

scholarly journals Phytochemical Investigation and Anti-Inflammatory Activity of the Leaves of Machilus japonica var. kusanoi

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4149
Author(s):  
Shiou-Ling Li ◽  
Ho-Cheng Wu ◽  
Tsong-Long Hwang ◽  
Chu-Hung Lin ◽  
Shuen-Shin Yang ◽  
...  
Keyword(s):  
Superoxide Anion ◽  
2D Nmr ◽  
Human Neutrophils ◽  
Superoxide Anion Generation ◽  
Phytochemical Investigation ◽  
Potent Inhibition ◽  
Bioassay Guided Fractionation ◽  
Ic50 Values ◽  
Anti Inflammatory ◽  
New Compounds

In a series of anti-inflammatory screenings of lauraceous plants, the methanolic extract of the leaves of Machilus japonica var. kusanoi (Hayata) J.C. Liao showed potent inhibition on both superoxide anion generation and elastase release in human neutrophils. Bioassay-guided fractionation of the leaves of M. japonica var. kusanoi led to the isolation of twenty compounds, including six new butanolides, machinolides A–F (1–6), and fourteen known compounds (7–20). Their structures were characterized by 1D and 2D NMR, UV, IR, CD, and MS data. The absolute configuration of the new compounds were unambiguously confirmed by single-crystal X-ray diffraction analyses (1, 2, and 3) and Mosher’s method (4, 5, and 6). In addition, lignans, (+)-eudesmin (11), (+)-methylpiperitol (12), (+)-pinoresinol (13), and (+)-galbelgin (16) exhibited inhibitory effects on N-formyl-methionyl-leucyl-phenylalanine/cytochalasin B (fMLP/CB)-induced superoxide anion generation in human neutrophils with IC50 values of 8.71 ± 0.74 μM, 2.23 ± 0.92 μM, 6.81 ± 1.07 μM, and 7.15 ± 2.26 μM, respectively. The results revealed the anti-inflammatory potentials of Formosan Machilus japonica var. kusanoi.

scholarly journals Anti-Inflammatory Cembranoids from a Formosa Soft Coral Sarcophyton cherbonnieri

Marine Drugs ◽  
2020 ◽  
Vol 18 (11) ◽  
pp. 573
Author(s):  
Chia-Chi Peng ◽  
Chiung-Yao Huang ◽  
Atallah F. Ahmed ◽  
Tsong-Long Hwang ◽  
Jyh-Horng Sheu
Keyword(s):  
Superoxide Anion ◽  
Soft Coral ◽  
Chemical Reduction ◽  
Chemical Constituents ◽  
2D Nmr ◽  
Human Neutrophils ◽  
Superoxide Anion Generation ◽  
Chemical Structures ◽  
Proinflammatory Responses ◽  
Anti Inflammatory

The present investigation on chemical constituents of the soft coral Sarcophyton cherbonnieri resulted in the isolation of seven new cembranoids, cherbonolides F–L (1–7). The chemical structures of 1–7 were determined by spectroscopic methods, including infrared, one- and two-dimensional (1D and 2D) NMR (COSY, HSQC, HMBC, and NOESY), MS experiments, and a chemical reduction of hydroperoxide by triphenylphosphine. The anti-inflammatory activities of 1–7 against neutrophil proinflammatory responses were evaluated by measuring their inhibitory ability toward N-formyl-methionyl-leucyl-phenylalanine/cytochalasin B (fMLF/CB)-induced superoxide anion generation and elastase release in primary human neutrophils. The results showed that all isolates exhibited moderate activities, while cherbonolide G (2) and cherbonolide H (3) displayed a more active effect than others on the inhibition of elastase release (48.2% ± 6.2%) and superoxide anion generation (44.5% ± 4.6%) at 30 µM, respectively.

Capping of silver nanoparticles by anti-inflammatory ligands: Antibacterial activity and superoxide anion generation

2019 ◽  
Vol 193 ◽  
pp. 100-108 ◽  
Author(s):  
Manuel I. Azócar ◽  
Romina Alarcón ◽  
Antonio Castillo ◽  
Jenny M. Blamey ◽  
Mariana Walter ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Superoxide Anion ◽  
Superoxide Anion Generation ◽  
Anti Inflammatory

New chemical constituents from the fruits of Zanthoxylum armatum and its in vitro anti-inflammatory profile

2017 ◽  
Vol 33 (5) ◽  
pp. 665-672 ◽  
Author(s):  
Zulfa Nooreen ◽  
Anant Kumar ◽  
Dnyaneshwar Umrao Bawankule ◽  
Sudeep Tandon ◽  
Mohd Ali ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Zanthoxylum Armatum ◽  
Anti Inflammatory ◽  
Inflammatory Profile

scholarly journals Metabolite Profiling of Aquilaria malaccensis Leaf Extract Using Liquid Chromatography-Q-TOF-Mass Spectrometry and Investigation of Its Potential Antilipoxygenase Activity In-Vitro

Processes ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 202 ◽  
Author(s):  
Manar A. Eissa ◽  
Yumi Z. H-Y. Hashim ◽  
Dina M. El-Kersh ◽  
Saripah S. S. Abd-Azziz ◽  
Hamzah Mohd. Salleh ◽  
...  
Keyword(s):  
Biological Activities ◽  
Chemical Constituents ◽  
Oral Epithelial Cells ◽  
Aquilaria Malaccensis ◽  
Ic50 Value ◽  
Mucosal Cells ◽  
Anti Inflammatory ◽  
Agarwood Resin ◽  
Inhibitory Potential

The Aquilaria malaccensis species of the genus Aquilaria is an abundant source of agarwood resin and many bioactive phytochemicals. Recent data regarding the chemical constituents and biological activities of Aquilaria leaves led us to attempt to qualitatively profile the metabolites of Aquilaria malaccensis leaves from a healthy, noninoculated tree through phytochemical screening, GC-MS, and LC/Q-TOF-MS. The present work is also the first to report the antilipoxygenase activity of A. malaccensis leaves from healthy noninoculated tree and investigate its toxicity on oral mucosal cells. A total of 53 compounds were tentatively identified in the extract, some of which have been described in literature as exhibiting anti-inflammatory activity. A number of compounds were identified for the first time in the extract of A. malaccensis leaf, including quercetin, quercetin-O-hexoside, kaempferol-O-dirhamnoside, isorhamnetin-O-hexoside, syringetin-O-hexoside, myricetin, tetrahydroxyflavanone, hesperetin, sissotrin, and lupeol. The antilipoxygenase assay was used to determine the lipoxygenase (LOX) inhibitory potential of the extract, while a WST-1 assay was conducted to investigate the effect of the extract on oral epithelial cells (OEC). The extract implied moderate anti-LOX activity with IC50 value of 71.6 µg/mL. Meanwhile, the cell viability of OEC ranged between 92.55% (10 µg/mL)–76.06% ± (100 µg/mL) upon treatment, indicating some potential toxicity risks. The results attained encourage future studies of the isolation of bioactive compounds from Aquilaria malaccensis leaves, as well as further investigation on the anti-inflammatory mechanisms and toxicity associated with their use.

scholarly journals Chemical Constituents from Scindapsus officinalis (Roxb.) Schott. and Their Anti–Inflammatory Activities

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2577 ◽  
Author(s):  
Hongjing Dong ◽  
Yanling Geng ◽  
Xueyong Wang ◽  
Xiangyun Song ◽  
Xiao Wang ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
2D Nmr ◽  
No Production ◽  
Inhibitory Effects ◽  
Nmr Data ◽  
Extensive Analysis ◽  
Ic50 Values ◽  
Monoterpene Glycoside ◽  
Anti Inflammatory

One new monoterpene glycoside (1), one new phenyl glycoside (2), one new caffeoyl derivative (3), were isolated from Scindapsus officinalis (Roxb.) Schott., along with four known compounds (4–7). Structures of the isolated compounds were elucidated by extensive analysis of spectroscopic data, especially 2D NMR data and comparison with literatures. All isolates were evaluated for anti-inflammatory activity against nitric oxide (NO) production in vitro. Compounds 3 and 7 exhibited moderate inhibitory effects on NO production with IC50 values of 12.2 ± 0.8 and 18.9 ± 0.3 μM, respectively.

scholarly journals IN VITRO PHARMACOLOGICAL SCREENING METHODS FOR ANTI-INFLAMMATORY AGENTS

2018 ◽  
Vol 11 ◽  
Author(s):  
Jayakumari S ◽  
Jijith Us
Keyword(s):  
Leukocyte Adhesion ◽  
Induced Response ◽  
Screening Methods ◽  
Adhesion Assay ◽  
Polymorphonuclear Cells ◽  
In Vitro Methods ◽  
Anti Inflammatory ◽  
Pharmacological Screening ◽  
New Compounds

Context: The development of in vitro methods is important in drug discovery process due to the restriction of animal use in the pharmacological screening process.Objective: The objective of the review was to understand the in vitro pharmacological screening methods for anti-inflammatory agents.Methods: The present review details various in vitro methods available for the screening of activity of therapeutic agents against inflammation.Results: Inhibition and measurement of nitric oxide production, mast cell degranulation, leukocyte adhesion assay, platelet-neutrophil adhesion assay, lipopolysaccharide-induced response assay, N-formyl-methionyl-leucyl-phenylalanine-induced O2 generation by polymorphonuclear cells, and cyclooxygenase assays are discussed in this review.Conclusions: These methods help to understand the real mechanism of inflammation and mediators involved in this process and help toidentify new compounds possessing the anti-inflammatory activity.

scholarly journals Chemical Constituents of Vigna luteola and Their Anti-inflammatory Bioactivity

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1371 ◽  
Author(s):  
Sio-Hong Lam ◽  
Yue-Chiun Li ◽  
Ping-Chung Kuo ◽  
Tsong-Long Hwang ◽  
Mei-Lin Yang ◽  
...  
Keyword(s):  
Superoxide Anion ◽  
Methanol Extract ◽  
Chemical Constituents ◽  
Mass Spectrometric ◽  
Inhibitory Effects ◽  
Potential Evaluation ◽  
Anti Inflammatory ◽  
Vigna Luteola

Seventy-three compounds were identified from the methanol extract of V. luteola, and among these, three new (1–3) were characterized by spectroscopic and mass spectrometric analyses. The isolated constituents were assessed for anti-inflammatory potential evaluation, and several purified principles exhibited significant superoxide anion and elastase inhibitory effects.

scholarly journals Chemical Constituents of the Leaves of Peltophorum pterocarpum and Their Bioactivity

Molecules ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 240 ◽  
Author(s):  
Yue-Chiun Li ◽  
Ping-Chung Kuo ◽  
Mei-Lin Yang ◽  
Tzu-Yu Chen ◽  
Tsong-Long Hwang ◽  
...  
Keyword(s):  
Superoxide Anion ◽  
Methanol Extract ◽  
Chemical Constituents ◽  
Mass Spectrometric ◽  
Inhibitory Effects ◽  
Superoxide Anion Generation ◽  
Chemical Structures

Two new sesquiterpenoids peltopterins A and B (compounds 1 and 2) and fifty-two known compounds were isolated from the methanol extract of P. pterocarpum and their chemical structures were established through spectroscopic and mass spectrometric analyses. The isolates 40, 43, 44, 47, 48, 51 and 52 exhibited potential inhibitory effects of superoxide anion generation or elastase release.

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