scholarly journals Anti-Quorum Sensing Activity of 12 Essential Oils on chromobacterium violaceum and Specific Action of cis-cis-p-Menthenolide from Corsican Mentha suaveolens ssp. Insularis

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2125 ◽  
Author(s):  
Jean-Pierre Poli ◽  
Elodie Guinoiseau ◽  
Dominique de Rocca Serra ◽  
Sylvain Sutour ◽  
Mathieu Paoli ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Essential Oils ◽  
Biofilm Formation ◽  
Inhibitory Concentration ◽  
Chromobacterium Violaceum ◽  
Sea Islands ◽  
Bioactive Substances ◽  
Bacterial Communication ◽  
Enhanced Resistance ◽  
Violacein Production

Quorum sensing (QS) is a bacterial communication mechanism used to express various survival or virulence traits leading to enhanced resistance. Chromobacterium violaceum is a commonly used strain that highlights anti-QS action of bioactive substances. Here, we wanted to see if 12 selected essential oils (EO) could exert anti-QS activity. We measured the sublethal minimal QS inhibitory concentration (MQSIC) by assessing violacein production of C. violaceum along with bacterial growth. To confirm the QS disruption, we also proceed to surface bacterial observations using scanning electron microscopy (SEM). We showed that cis-cis-p-menthenolide extracted and isolated from a plant endemic to occidental Mediterranean Sea islands, Mentha suaveolens ssp. insularis, acts as an inhibitor of violacein production and biofilm formation. Measured MQSIC was much lower than the minimal inhibitory concentration (MIC): 0.10 mg·mL−1 vs. 3.00 mg·mL−1. Moreover, disturbance of QS-related traits was confirmed by the degradation of C. violaceum biofilm matrix. There is a clear structure–activity relationship between cis-cis-p-menthenolide and anti-QS activity. Indeed, its isomer molecule (mintlactone) exerts a poor anti-QS action. These results indicate that inhibition of violacein production and biofilm formation by cis-cis-p-menthenolide might be related to a disruption in the QS mechanism.

scholarly journals Chromobacterium violaceum and Pseudomonas aeruginosa PAO1: Models for Evaluating Anti-Quorum Sensing Activity of Melaleuca alternifolia Essential Oil and Its Main Component Terpinen-4-ol

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2672 ◽  
Author(s):  
Emira Noumi ◽  
Abderrahmen Merghni ◽  
Mousa M. Alreshidi ◽  
Ons Haddad ◽  
Gültekin Akmadar ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Essential Oil ◽  
Biofilm Formation ◽  
Chromobacterium Violaceum ◽  
Melaleuca Alternifolia ◽  
Swarming Motility ◽  
Pseudomonas Aeruginosa Pao1 ◽  
Tea Tree ◽  
Violacein Production

The problem of antibiotic resistance among pathogens encourages searching for novel active molecules. The aim of the research was to assay the anti-quorum sensing (anti-QS) and antibiofilm potential of Melaleuca alternifolia essential oil and its main constituent, terpinen-4-ol, to prevent the infections due to methicillin-resistant Staphylococcus aureus strains as an alternate to antibiotics. The tea tree oil (TTO) was evaluated for its potential in inhibiting QS-dependent phenomena such as violacein production in Chromobacterium violaceum, swarming motility of Pseudomonas aeruginosa PAO1, and biofilm formation in MRSA strains on glass. The results showed that terpinen-4-ol was able to inhibit MRSA strain biofilm formation on the glass strips by 73.70%. TTO inhibited the violacein production at a mean inhibitory concentration (MIC) value of 0.048 mg/mL by 69.3%. At 100 µg/mL TTO and terpinen-4-ol exhibited inhibition in swarming motility of PAO1 by 33.33% and 25%, respectively. TTO revealed anti-QS and anti-biofilm activities at very low concentrations, but it could be further investigated for new molecules useful for the treatment of MRSA infections.

scholarly journals Evaluation of Enzyme Inhibition and Anti-Quorum Sensing Potentials of Melaleuca alternifolia and Citrus sinensis Essential Oils

2021 ◽  
Vol 16 (9) ◽  
pp. 1934578X2110445
Author(s):  
Tamfu Alfred Ngenge ◽  
Selcuk Kucukaydin ◽  
Ozgur Ceylan ◽  
Mehmet Emin Duru
Keyword(s):  
Quorum Sensing ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Chemical Compositions ◽  
Tea Tree Oil ◽  
Melaleuca Alternifolia ◽  
Tea Tree ◽  
Inhibition Concentration ◽  
Violacein Production ◽  
Urease Inhibitory

In this study, Melaleuca alternifolia (Maiden and Betche) Cheel (tea tree) oil and C. sinensis (L.) Osbeck (orange) oil was procured, and their chemical compositions were characterized by gas chromatography coupled with mass spectrometry, with co-injection using authentic samples. The oils were rich in monoterpene hydrocarbons making up 84.5% of C. sinensis and 48.9% of M. alternifolia oils. High amounts of oxygenated monoterpenoids were also identified in C. sinensis (9.6%) and M. alternifolia (49.3%) oils. The three most abundant compounds were limonene (71.2%), linalool (5.5%), and β-myrcene (5.1%) in C. sinensis, and terpinen-4-ol (45.6%), γ-terpinene (19.4%) and α-terpinene (9.3%) in M. alternifolia. Enzyme inhibitions (anticholinesterase, antiurease, antityrosinase) of both essential oils were evaluated. In acetylcholinesterase assay, M. alternifolia and C. sinensis had inhibition concentration (IC50) values of 153.7 ± 1.25 and 96.4 ± 1.0 µg/mL, respectively as compared to 5.42 ± 0.11 µg/mL for galantamine, while in butyrylcholinesterase assay, M. alternifolia (IC50 = 85.6 ± 0.7 µg/mL) and C. sinensis (IC50 = 127.8 ± 0.6 µg/mL) exhibited moderate activities compared to galantamine (IC50 = 45.8 ± 0.8 µg/mL). In the urease inhibitory activity, essential oils of C. sinensis and M. alternifolia showed 48.4% ± 0.9% and 30.6% ± 0.7% inhibitions at 200 µg/mL concentration, respectively. Quorum sensing (QS) mediated violacein production in Chromobacterium violaceum CV12472 was inhibited by 100% at minimum inhibitory concentration (MIC) values for both oils while showing QS inhibition diameter zones in C. violaceum of 22.5 ± 0.4 mm and 14.3 ± 0.5 mm for C. sinensis and M. alternifolia oils, respectively at MIC concentration. The good quorum-sensing potential indicates that these oils can suppress microbial resistance and severity of infections.

Citral and its derivatives inhibit quorum sensing and biofilm formation in Chromobacterium violaceum

2021 ◽  
Author(s):  
Nikayla Batohi ◽  
Shabir Ahmad Lone ◽  
Musa Marimani ◽  
Mohmmad Younus Wani ◽  
Abdullah Saad Al-Bogami ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Chromobacterium Violaceum

scholarly journals Anti-biofilm and Virulence Factor-Reducing Activities of Essential Oils and Oil Components as a Possible Option for Bacterial Infection Control

Planta Medica ◽  
2020 ◽  
Vol 86 (08) ◽  
pp. 520-537 ◽  
Author(s):  
Jürgen Reichling
Keyword(s):  
Quorum Sensing ◽  
Essential Oil ◽  
Essential Oils ◽  
Virulence Factor ◽  
Model Organisms ◽  
Regulation System ◽  
Expression Of Genes ◽  
Reporter Strains ◽  
Violacein Production ◽  
Essential Oil Compounds

AbstractPathogenic biofilm-associated bacteria that adhere to biological or nonbiological surfaces are a big challenge to the healthcare and food industries. Antibiotics or disinfectants often fail in an attempt to eliminate biofilms from those surfaces. Based on selected experimental research, this review deals with the potential biofilm-inhibiting, virulence factor-reducing, and biofilm-eradicating activities of essential oils and single essential oil compounds using Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Chromobacterium violaceum as model organisms. In addition, for the bacteria reviewed in this overview, different essential oils and essential oil compounds were reported to be able to modulate the expression of genes that are involved in the formation of autoinducer molecules, biofilms, and virulence factors. The anti-quorum sensing activity of some essential oils and single essential oil compounds was demonstrated using the gram-negative bacterium C. violaceum. Reporter strains of this bacterium produce the violet-colored compound violacein whose synthesis is regulated by quorum sensing autoinducer molecules called acylhomeserinlactones. Of great interest was the discovery that enantiomeric monoterpenes affected the quorum sensing regulation system in different ways. While the (+)-enantiomers of carvone, limonene, and borneol increased violacein formation, their (−)-analogues inhibited violacein production.For the successful eradication of biofilms and the bacteria living inside them, it is absolutely necessary that the lipophilic volatile substances can penetrate into the aqueous channels of biofilms. As shown in recent work, hydrophilic nano-delivery systems encapsulating essential oils/essential oil compounds with antibacterial effects may contribute to overcome this problem.

scholarly journals Antimicrobial and antibiofilm properties of essential oils from Piper marginatum Jacq.

2021 ◽  
Vol 10 (11) ◽  
pp. e514101119967
Author(s):  
Ana Lúcia Mendes dos Santos ◽  
Filipe Augusto Matos Araújo ◽  
Érika da Silva Matisui ◽  
Luiz Antonio Mendonça Alves da Costa ◽  
Alexandre José Macêdo ◽  
...  
Keyword(s):  
Essential Oils ◽  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Folk Medicine ◽  
Gram Negative Bacteria ◽  
Biofilm Inhibition ◽  
Future Developments ◽  
Phytochemical Constituents ◽  
Amazonian Region

A low shrub growing in the Amazonian region, Piper marginatum Jacq. has been related to the treatment of a disease variety in folk medicine, however, still lacking scientific support. This study aimed to describe the composition of essential oils obtained from leaves (EOL) and branches (EOB) of P. marginatum and their antimicrobial effects on six relevant pathogenic bacteria. A combination of GC-FID and GC-MS was used to identify the phytochemical constituents. As antimicrobial assays, the oils were screened at the minimum inhibitory concentration (MIC) of 3 µg/ml for planktonic and biofilm inhibition. EOL revealed the presence of trans–nerolidol, o–cymene, spathulenol, elemicin, and α–copaene, while EOB composition was mainly of myristicin, trans-caryophyllene, trans-nerolidol, caryophyllene oxide, α–copaene, γ–muurolene and spathulenol. The strongest inhibition of planktonic growth was achieved against Pseudomonas aeruginosa (EOB) and Escherichia coli (EOB). Overall, Gram negative bacteria were more sensitive to both EOB/EOL showing less ability of growth and biofilm formation. The Gram-positive strains seemed to react to the essential oils by massive adhesion. Our results corroborate the relevance of Piperaceae and indicate the possible use of P. marginatum in future developments of antimicrobials.

scholarly journals IgY Targeting Bacterial Quorum-Sensing Molecules in Implant-Associated Infections

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 4027
Author(s):  
Ulrike Dapunt ◽  
Birgit Prior ◽  
Christopher Oelkrug ◽  
Jan Philippe Kretzer
Keyword(s):  
Quorum Sensing ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Major Complication ◽  
Underlying Mechanism ◽  
Bacterial Communication ◽  
Bacterial Quorum Sensing ◽  
Polysaccharide Intercellular Adhesion ◽  
Bacterial Quorum ◽  
Treatment Alternatives

Background: Implant-associated infections are still a major complication in the field of orthopedics. Bacteria can form biofilms on implant surfaces, making them more difficult to detect and treat. Since standard antibiotic therapy is often impaired in biofilm infections, particular interest is directed towards finding treatment alternatives. Biofilm-formation is a well-organized process during which bacteria communicate via quorum-sensing molecules (QSM). The aim of this study was to inhibit bacterial communication by directing avian IgY against specific QSM. Methods: Chicken were immunized against the following QSM: (1) AtlE, a member of the autolysin family which mediates attachment to a surface in Staphylococcus epidermidis; (2) GroEL, the bacterial heat shock protein; (3) PIA (polysaccharide intercellular adhesion), which is essential for cell–cell adhesion in biofilms. Staphylococcus epidermidis biofilms were grown and inhibition of biofilm-formation by IgYs was evaluated. Additionally, human osteoblasts were cultivated and biocompatibility of IgYs was tested. Results: We were able to demonstrate that all IgYs reduced biofilm-formation, also without prior immunization. Therefore, the response was probably not specific with regard to the QSM. Osteoblasts were activated by all IgYs which was demonstrated by microscopy and an increased release of IL-8. Conclusions: In conclusion, avian IgY inhibits biofilm-formation, though the underlying mechanism is not yet clear. However, adverse effects on local tissue cells (osteoblasts) were also observed.

scholarly journals Highly branched poly(N-isopropyl acrylamide) functionalized with an inducer molecule suppresses quorum sensing in Chromobacterium violaceum

2019 ◽  
Vol 55 (66) ◽  
pp. 9765-9768 ◽  
Author(s):  
Joanna Shepherd ◽  
Thomas Swift ◽  
Chien-Yi Chang ◽  
James R. Boyne ◽  
Stephen Rimmer ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Mode Of Action ◽  
Biofilm Formation ◽  
Chromobacterium Violaceum ◽  
Isopropyl Acrylamide ◽  
Bacterial Quorum Sensing ◽  
Bacterial Quorum

Bacterial quorum sensing has been implicated in a number of pathogenic bacterial processes, such as biofilm formation, making it a crucial target for developing materials with a novel antibiotic mode of action.

scholarly journals Selenium and tellurium-based nanoparticles as interfering factors in quorum sensing-regulated processes: violacein production and bacterial biofilm formation

Metallomics ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 1104-1114 ◽  
Author(s):  
Beatriz Gómez-Gómez ◽  
Lucia Arregui ◽  
Susana Serrano ◽  
Antonio Santos ◽  
Teresa Pérez-Corona ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Bacterial Biofilm ◽  
Persistent Infections ◽  
Violacein Production ◽  
New Strategies

The effect of SeNPs and TeNPs on different processes regulated by QS such as violacein production and biofilm formation is presented. The data open new strategies for controlling persistent infections.

Tannin-Rich Fraction from Pomegranate Rind Inhibits Quorum Sensing in Chromobacterium violaceum and Biofilm Formation in Escherichia coli

2016 ◽  
Vol 13 (1) ◽  
pp. 28-35 ◽  
Author(s):  
Qinnan Yang ◽  
Lingfang Wang ◽  
Jianxue Gao ◽  
Xiaobo Liu ◽  
Yuqing Feng ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Quorum Sensing ◽  
Biofilm Formation ◽  
Chromobacterium Violaceum

scholarly journals Effects of Natural Products on Bacterial Communication and Network-Quorum Sensing

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Min Yang ◽  
Fanying Meng ◽  
Wen Gu ◽  
Fengjiao Li ◽  
Yating Tao ◽  
...  
Keyword(s):  
Natural Products ◽  
Quorum Sensing ◽  
Virulence Factor ◽  
Biofilm Formation ◽  
Bacterial Flora ◽  
External Environment ◽  
Cell Communication ◽  
Beneficial Bacteria ◽  
Bacterial Communication ◽  
Virulence Factor Production

Quorum sensing (QS) has emerged as a research hotspot in microbiology and medicine. QS is a regulatory cell communication system used by bacterial flora to signal to the external environment. QS influences bacterial growth, proliferation, biofilm formation, virulence factor production, antibiotic synthesis, and environmental adaptation. Through the QS system, natural products can regulate the growth of harmful bacteria and enhance the growth of beneficial bacteria, thereby improving human health. Herein, we review advances in the discovery of natural products that regulate bacterial QS systems.

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