Tannin-Rich Fraction from Pomegranate Rind Inhibits Quorum Sensing in Chromobacterium violaceum and Biofilm Formation in Escherichia coli

2016 ◽  
Vol 13 (1) ◽  
pp. 28-35 ◽  
Author(s):  
Qinnan Yang ◽  
Lingfang Wang ◽  
Jianxue Gao ◽  
Xiaobo Liu ◽  
Yuqing Feng ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Quorum Sensing ◽  
Biofilm Formation ◽  
Chromobacterium Violaceum

Citral and its derivatives inhibit quorum sensing and biofilm formation in Chromobacterium violaceum

2021 ◽  
Author(s):  
Nikayla Batohi ◽  
Shabir Ahmad Lone ◽  
Musa Marimani ◽  
Mohmmad Younus Wani ◽  
Abdullah Saad Al-Bogami ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Chromobacterium Violaceum

scholarly journals Highly branched poly(N-isopropyl acrylamide) functionalized with an inducer molecule suppresses quorum sensing in Chromobacterium violaceum

2019 ◽  
Vol 55 (66) ◽  
pp. 9765-9768 ◽  
Author(s):  
Joanna Shepherd ◽  
Thomas Swift ◽  
Chien-Yi Chang ◽  
James R. Boyne ◽  
Stephen Rimmer ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Mode Of Action ◽  
Biofilm Formation ◽  
Chromobacterium Violaceum ◽  
Isopropyl Acrylamide ◽  
Bacterial Quorum Sensing ◽  
Bacterial Quorum

Bacterial quorum sensing has been implicated in a number of pathogenic bacterial processes, such as biofilm formation, making it a crucial target for developing materials with a novel antibiotic mode of action.

Quorum sensing is crucial to Escherichia coli O157:H7 biofilm formation under static or very slow laminar flow conditions

2016 ◽  
Vol 10 (3) ◽  
pp. 241-249 ◽  
Author(s):  
Jeesun Lim ◽  
Kang-Mu Lee ◽  
Chan Yong Park ◽  
Han Vit Kim ◽  
Younghoon Kim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Quorum Sensing ◽  
Laminar Flow ◽  
Biofilm Formation ◽  
Escherichia Coli O157 ◽  
Flow Conditions ◽  
Laminar Flow Conditions

scholarly journals Role of Quorum Sensing and Antimicrobial Component Production by Serratia plymuthica in Formation of Biofilms, Including Mixed Biofilms with Escherichia coli

2006 ◽  
Vol 72 (11) ◽  
pp. 7294-7300 ◽  
Author(s):  
Pieter Moons ◽  
Rob Van Houdt ◽  
Abram Aertsen ◽  
Kristof Vanoirbeek ◽  
Yves Engelborghs ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Quorum Sensing ◽  
Biofilm Formation ◽  
Homoserine Lactone ◽  
Broth Culture ◽  
Confocal Laser ◽  
Wild Type ◽  
Serratia Plymuthica ◽  
E Coli

ABSTRACT We have previously characterized the N-acyl-l-homoserine lactone-based quorum-sensing system of the biofilm isolate Serratia plymuthica RVH1. Here we investigated the role of quorum sensing and of quorum-sensing-dependent production of an antimicrobial compound (AC) on biofilm formation by RVH1 and on the cocultivation of RVH1 and Escherichia coli in planktonic cultures or in biofilms. Biofilm formation of S. plymuthica was not affected by the knockout of splI or splR, the S. plymuthica homologs of the luxI or luxR quorum-sensing gene, respectively, or by the knockout of AC production. E. coli grew well in mixed broth culture with RVH1 until the latter reached 8.5 to 9.5 log CFU/ml, after which the E. coli colony counts steeply declined. In comparison, only a very small decline occurred in cocultures with the S. plymuthica AC-deficient and splI mutants. Complementation with exogenous N-hexanoyl-l-homoserine lactone rescued the wild-type phenotype of the splI mutant. The splR knockout mutant also induced a steep decline of E. coli, consistent with its proposed function as a repressor of quorum-sensing-regulated genes. The numbers of E. coli in 3-day-old mixed biofilms followed a similar pattern, being higher with S. plymuthica deficient in SplI or AC production than with wild-type S. plymuthica, the splR mutant, or the splI mutant in the presence of N-hexanoyl-l-homoserine lactone. Confocal laser scanning microscopic analysis of mixed biofilms established with strains producing different fluorescent proteins showed that E. coli microcolonies were less developed in the presence of RVH1 than in the presence of the AC-deficient mutant.

Flagellin and F4 fimbriae have opposite effects on biofilm formation and quorum sensing in F4ac+ enterotoxigenic Escherichia coli

2014 ◽  
Vol 168 (1) ◽  
pp. 148-153 ◽  
Author(s):  
Mingxu Zhou ◽  
Zhiyan Guo ◽  
Yang Yang ◽  
Qiangde Duan ◽  
Qi Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Quorum Sensing ◽  
Biofilm Formation ◽  
Enterotoxigenic Escherichia Coli

scholarly journals Anti-Quorum Sensing Activity of 12 Essential Oils on chromobacterium violaceum and Specific Action of cis-cis-p-Menthenolide from Corsican Mentha suaveolens ssp. Insularis

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2125 ◽  
Author(s):  
Jean-Pierre Poli ◽  
Elodie Guinoiseau ◽  
Dominique de Rocca Serra ◽  
Sylvain Sutour ◽  
Mathieu Paoli ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Essential Oils ◽  
Biofilm Formation ◽  
Inhibitory Concentration ◽  
Chromobacterium Violaceum ◽  
Sea Islands ◽  
Bioactive Substances ◽  
Bacterial Communication ◽  
Enhanced Resistance ◽  
Violacein Production

Quorum sensing (QS) is a bacterial communication mechanism used to express various survival or virulence traits leading to enhanced resistance. Chromobacterium violaceum is a commonly used strain that highlights anti-QS action of bioactive substances. Here, we wanted to see if 12 selected essential oils (EO) could exert anti-QS activity. We measured the sublethal minimal QS inhibitory concentration (MQSIC) by assessing violacein production of C. violaceum along with bacterial growth. To confirm the QS disruption, we also proceed to surface bacterial observations using scanning electron microscopy (SEM). We showed that cis-cis-p-menthenolide extracted and isolated from a plant endemic to occidental Mediterranean Sea islands, Mentha suaveolens ssp. insularis, acts as an inhibitor of violacein production and biofilm formation. Measured MQSIC was much lower than the minimal inhibitory concentration (MIC): 0.10 mg·mL−1 vs. 3.00 mg·mL−1. Moreover, disturbance of QS-related traits was confirmed by the degradation of C. violaceum biofilm matrix. There is a clear structure–activity relationship between cis-cis-p-menthenolide and anti-QS activity. Indeed, its isomer molecule (mintlactone) exerts a poor anti-QS action. These results indicate that inhibition of violacein production and biofilm formation by cis-cis-p-menthenolide might be related to a disruption in the QS mechanism.

scholarly journals Effect of Bromination on the Quorum Sensing-Inhibiting Properties of Indole-3-Carboxaldehydes in Chromobacterium violaceum AHL System

2021 ◽  
Vol 12 (2) ◽  
pp. 376-382
Author(s):  
Chesley A. Kemp ◽  
Donna K. McCullough ◽  
Dobrusia Bialonska ◽  
Paul J. T. Johnson
Keyword(s):  
Escherichia Coli ◽  
Quorum Sensing ◽  
Virulence Factors ◽  
Indole Derivative ◽  
Chromobacterium Violaceum ◽  
Quorum Sensing Inhibition ◽  
Signaling Molecule ◽  
Bacterial Communication ◽  
Ic50 Value ◽  
Ic50 Values

Quorum sensing (QS) is a form of bacterial communication involved in the production of virulence factors in many species. As a result, inhibition of quorum sensing may be of use in mitigating pathogenesis. The signaling molecule indole is currently being investigated as a target for quorum sensing inhibition (QSI) and the indole derivative indole-3-carboxaldehyde (ICA) has been shown to inhibit quorum sensing-mediated behaviors in Escherichia coli. In this study, we investigate bromination as a method of increasing the QSI capabilities of indole carboxaldehydes. The IC50 values of three monobrominated indole carboxaldehydes (5-bromoindole-3-carboxaldehyde, 6-bromoindole-3-carboxaldehyde, and 7-bromoindole-3-carboxaldehyde) were determined and compared to the IC50 value of ICA. The bromination of these indole carboxaldehydes reduced the IC50 values between 2- and 13-fold, indicating that bromination significantly increases the potency of these indole carboxaldehydes.

scholarly journals Reconfiguring the Quorum-Sensing Regulator SdiA of Escherichia coli To Control Biofilm Formation via Indole and N-Acylhomoserine Lactones

2009 ◽  
Vol 75 (6) ◽  
pp. 1703-1716 ◽  
Author(s):  
Jintae Lee ◽  
Toshinari Maeda ◽  
Seok Hoon Hong ◽  
Thomas K. Wood
Keyword(s):  
Escherichia Coli ◽  
Quorum Sensing ◽  
Biofilm Formation ◽  
Transcriptome Profiling ◽  
Homoserine Lactone ◽  
Wild Type ◽  
Acylhomoserine Lactone ◽  
Induced Genes ◽  
Whole Transcriptome ◽  
Indole Synthesis

ABSTRACT SdiA is a homolog of quorum-sensing regulators that detects N-acylhomoserine lactone (AHL) signals from other bacteria. Escherichia coli uses SdiA to reduce its biofilm formation in the presence of both AHLs and its own signal indole. Here we reconfigured SdiA (240 amino acids) to control biofilm formation using protein engineering. Four SdiA variants were obtained with altered biofilm formation, including truncation variants SdiA1E11 (F7L, F59L, Y70C, M94K, and K153X) and SdiA14C3 (W9R, P49T, N87T, frameshift at N96, and L123X), which reduced biofilm formation by 5- to 20-fold compared to wild-type SdiA in the presence of endogenous indole. Whole-transcriptome profiling revealed that wild-type SdiA reduced biofilm formation by repressing genes related to indole synthesis and curli synthesis compared to when no SdiA was expressed, while variant SdiA1E11 induced genes related to indole synthesis in comparison to wild-type SdiA. These results suggested altered indole metabolism, and corroborating the DNA microarray results in regard to indole synthesis, variant SdiA1E11 produced ninefold more indole, which led to reduced swimming motility and cell density. Also, wild-type SdiA decreased curli production and tnaA transcription, while SdiA1E11 increased tnaA transcription (tnaA encodes tryptophanase, which forms indole) compared to wild-type SdiA. Hence, wild-type SdiA decreased biofilm formation by reducing curli production and motility, and SdiA1E11 reduced biofilm formation via indole. Furthermore, an AHL-sensitive variant (SdiA2D10, having four mutations at E31G, Y42F, R116H, and L165Q) increased biofilm formation sevenfold in the presence of N-octanoyl-dl-homoserine lactone and N-(3-oxododecatanoyl)-l-homoserine lactone. Therefore, SdiA can be evolved to increase or decrease biofilm formation, and biofilm formation may be controlled by altering sensors rather than signals.

Rutin inhibits quorum sensing, biofilm formation and virulence genes in avian pathogenic Escherichia coli

2018 ◽  
Vol 119 ◽  
pp. 54-59 ◽  
Author(s):  
Lu-Yuan Peng ◽  
Meng Yuan ◽  
Zhen-Qiang Cui ◽  
Zong-Mei Wu ◽  
Zhen-Jiang Yu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Quorum Sensing ◽  
Biofilm Formation ◽  
Virulence Genes ◽  
Avian Pathogenic Escherichia Coli ◽  
Pathogenic Escherichia Coli
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